• Korean Journal of Microbiology
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• v.28 no.2
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• pp.91-97
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• 1990

Complementary DNA (cDNA) coding for human cytoplasmic superoxide dismutase (SOD1) (superoxide: superoxide oxidoreductase E.C.1.15.1.1) was isolated from human liver cDNA library of $\lambda$gt11 by in situ plaque hybridization. The insery cDNA gas the 5' untranslational region (UTR) and 3'UTR of SOD1 gene. Polymerase Chain Reaction (PCR) method was used fro subcloning of SOD1 structural gene. Using synthetic sense strand primer (24mer) containing a start codon and antisense strand primer (24mer), SOD1 structural gene was selectively amplified. Amplified DNA was directly cloned into the HincII site of pUC19 plasmid. Insery cDNA was subcloned into M13 mp19 and sequenced by dideowy chain termination method with Sequenase. The nucleotide sequence of insert cDNA had an open reading frame (ORF) coding for 153 amino acid residues. The structural gene of cytoplasmic SOD was placed under the control of bacteriophage $\lambda P_{L}$ regulatory sequences, generating a highly efficient expression plasmid. The production of human SOD1 in E. coli cells was about 7% of total cellular proteins and recombinant human SOD1 possessed its own enzymatic acitivity.

• Journal of Korean Tunnelling and Underground Space Association
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• v.24 no.6
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• pp.699-714
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• 2022

The objective of this study is to suggest a safe and economical pillar reinforcement method when very near-twin tunnels with a minimum interval of 1 m passes through a soft zone such as weathered soil or weathered rock. A standard cross-sectional view of a two-lane road tunnel was applied to suggest a pillar reinforcement method for the very near-twin tunnels. The thickness of the pillar was 1 m. The ground condition around the tunnel was weathered soil or weathered rock. There were four reinforcement methods for pillar stability evaluation. These were rock bolt reinforcement, pre-stressed steel strand reinforcement, horizontal steel pipe grouting reinforcement, horizontal steel pipe grouting + prestressed steel strand reinforcement. When the ground condition was weathered soil, only the pillar reinforced the horizontal steel pipe grouting + prestressed steel strand did not failed. When the ground condition was weathered rock, there were no failure of the pillar reinforced the horizontal steel pipe grouting or the horizontal steel pipe grouting + prestressed steel strand. It is considered that the horizontal steel pipe grouting reinforcement played a role in increasing the stability of the upper part of the pillar by supporting the upper load applied to the upper part of the pillar.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.1
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• pp.47-59
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• 2013

Ultimate load factors of PPWS(Prefabricated Parallel Wire Strand) sockets in main cables of suspension bridges are studied with respect to the tapered angles of the inner surface of sockets. After briefly reviewing the current design method, 15 numbers of finite element models of sockets are prepared by varying the number of wires in a strand and the tapered angles. The finite element models are updated by comparing experimental and numerical results, so that the models can reflect the real behavior of sockets. The stress distributions at the first yielding and ultimate states are analyzed by performing the incremental load analysis using ABAQUS. It is concluded that the optimized tapered angle of sockets should be determined at the specific angle between the results of verification equations of the required bonding length and stress resistance length.

• Journal of the Korean Wood Science and Technology
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• v.46 no.5
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• pp.425-436
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• 2018

This study was conducted to evaluate the planar (rolling) shear strength of OSB (oriented strand board) panels and domestic plywood through 5 point bending test method in ASTM D2718 standard. The test specimens were prepared in parallel and perpendicular direction to major axis (along the length of panels) and tested up to failure, and failure modes were also examined. From the test results, rolling shear strength were found to be $1.32{\sim}1.94N/mm^2$ in parallel to major axis, and $1.46{\sim}1.99N/mm^2$ in perpendicular to major axis respectively. Little difference was found between parallel and perpendicular direction of rolling shear strength. There were no statistically significant differences in rolling shear strength between Canadian OSB and domestic plywood in the parallel direction, and between Chilean OSB and domestic plywood in the perpendicular direction. The shear failure was observed in all tested OSB panels, whereas shear failure, glue line delamination, and bending combined with shear failure were observed in the domestic plywood.

• Analytical Science and Technology
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• v.17 no.1
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• pp.53-59
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• 2004

Up to now, methods for the detection of genetic alterations as single strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE) have been used. It is too labor-intensive and expensive to serve for routine analysis. Moreover, lower in its sensitivity and specificity being also strongly dependent on the experience of the investigater. To improve these problems, we analysed mutation of ${\beta}_2$-adrenergic receptor gene that controls bronchial asthma by denaturing high performance liquid chromatography (DHPLC) according to ion-pair reversed phase chromatography (IP-RPC). We extracted genomic DNA from 80 asthma patients and then amplified DNA using PCR and analysed PCR product by SSCP and DHPLC. As a result, we analysed mutation frequency is 19 (23.75%) on SSCP and 25 (31.25%) on DHPLC in ${\beta}_2$-adrenergic receptor gene. We conclude that DHPLC is a fast and simple screening method rather than SSCP analysis.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.4
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• pp.395-401
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• 2022

In this study, the transfer length of strand was measured for three types of HCS member(H200, H320, and H400) manufactured by the pretension method. Strain gauges were attached in longitudinal direction at regular intervals on the sides of the HCS members, and the strain was measured during the cutting process of HCS. The stain at the cutting point was zero, and gradually increases in the central direction of the member, converging to a constant value after passing the transfer length. In the case of H200 members in which the strands were arranged one by one, the transfer lengths were formed within the range of the design equation (up to 762 mm). The transfer length of the H320 member and the H400 member, in which three strands were arranged, was higher than the design range (850 mm or more).

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.5A
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• pp.747-756
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• 2008

The purpose of this paper is to investigate the effect of the important parameters on the transfer length during the dynamic flame cutting of tendon experimentally. The considered parameters were strand diameter, concrete cover thickness, stirrup, debonding strand and release method. Ten pretensioned concete beam specimens were cast and tested. Time history curves for the axial strain of tendon were measured by electrical resistance strain gauges mounted on the strands. Experimental results indicated that large dynamic shock effects occurred near cut-end during the sudden release. The prestressing forces are dependent on the parameters above considered. The ratio of residual prestressing forces of 12.7 mm strands is greater than 15.2 mm strands. Using debonding strand and gradual release are more efficient for applying prestressing forces.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.10
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• pp.118-127
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• 2014

DNA data storage refers to any technique for storing massive digital data in base sequence of DNA and has been recognized as the future storage medium recently. This paper presents an information hiding method for DNA data storage that the massive data is hidden in non-coding strand based on DNA steganography. Our method maps the encrypted data to the data base sequence using the numerical mapping table and then hides it in the non-coding strand using the key that consists of the seed and sector length. Therefore, our method can preserve the protein, extract the hidden data without the knowledge of host DNA sequence, and detect the position of mutation error. Experimental results verify that our method has more high data capacity than conventional methods and also detects the positions of mutation errors by the parity bases.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.214-221
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• 2001

The Great Hall of Incheon International Airport Transportation Center has irregular curved roof structure. The structure consists of 13 main steel trusses. (the longest span of 162m, 480ton) The total weight of the truss is 6,300ton and the whole truss is made of 9,600 pieces which has the joint connected to maximum 13 different parts at one point and Roof Truss is supported by 12 Fabric Foundation. Considering the economical efficiency and the schedule, the Sliding Construction Method was used other than conventional erection method. The roof truss structure was divided into two blocks, 3,550ton and 2,700ton, each block was pre-erected on the Giant Sleigh off the site and was pulled 181m by using Tandem Pulling Jack and Strand to be set in place.

• Structural Engineering and Mechanics
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• v.18 no.4
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• pp.475-491
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• 2004

Anchorage devices play an important role in post-tensioned bridge structures since they must sustain heavy loads in order to permit the transfer of the prestressing force to the structure. In external prestressing, the situation is even more critical since the anchorage mechanisms, with the deviators, are the only links between the structure and the tendons throughout the service life of the structure. The behaviour of anchorage devise may be studied by using the finite element method. To do so, each component of the anchorage must be adequately represented in order to approximate the anchor mechanism as accurately as possible. In particular, the modelling of the jaw/tendon device may be carried out using the real geometry of these two components with an appropriate constitutive contact law or by replacing these components by a single equivalent. This paper presents the numerical study of a mono-strand anchorage device. The results of a comparison between two different representations of the jaw/tendon device, either as two distinct components or as a single equivalent, will be examined. In the double-component setup, the influence of the wedge configuration composing the jaw, and the influence of lubrication of the anchor, will be assessed.