• Title/Summary/Keyword: Strain-control

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Isolation and Identification of Burkholderia pyrrocinia CH-67 to Control Tomato Leaf Mold and Damping-off on Crisphead Lettuce and Tomato

  • Lee, Kwang-Youll;Kong, Hyun-Gi;Choi, Ki-Hyuck;Lee, Seon-Woo;Moon, Byung-Ju
    • The Plant Pathology Journal
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    • v.27 no.1
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    • pp.59-67
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    • 2011
  • A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

Isolation, Identification and Biological Control Activity of SKU-78 Strain against Ralstonia solanacearum (풋마름병균, Ralstonia solanacearum의 길항세균 SKU-78 균주의 분리 동정 및 특성)

  • Sung, Pil-Je;Shin, Jeong-Kun;Cho, Hong-Bum;Kim, Shin-Duk
    • Applied Biological Chemistry
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    • v.48 no.1
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    • pp.48-52
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    • 2005
  • Six stains of plant growth promoting rhizobacteria were selected through germinating seed assay and root colonization assay. Among them, SKU-78 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 60% reduction of bacterial wilt disease compared with the control. It was suggested that SKU-78 strain activated the host defense systems in plants, based on lack of direct antibiosis against pathogen. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, SKU-78 stain was identified as Bacillus sp. SKU-78.

A Study on Residual stress at Cutting work (절삭가공시 잔류응력에 관한 연구)

  • 주호윤
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.6 no.3
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    • pp.111-117
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    • 1997
  • The sudden-stop apparatus is made to measure the residual stress of the infinitesimal area at the turning work surface by using the X-ray stress apparatus. This study is trued to make the cutting work the instantaneous stopping state in the normal working state. The behaviour of work material near the tool is estimated. The estimation method is that the distribution of residual stress can be also measured. The object is to clarify and control the mechanism to leave the adequate stress of the finishing surface. It's beginning is due to observe the occurrence state of the residual stress at the cutting work. The result obtained by this study is as follows. The chips are not separated from the work materials at all the cutting experiments of built-up edges or the shearing areas etc. which can be precisely observed by using the sudden-stop apparatus. The strain of movable system which can be seen at the part of working layer means the size of strain. This experiment proves that the working strain should be lessened to make the size of strain control the residual stress happened at the cutting surface.

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Effect of Sports Taping on Impact Forces and Muscle Tuning during Drop Landing (드롭 착지 시 스포츠 테이핑이 하지의 충격력과 근육 조율에 미치는 영향)

  • Kang, Nyeon-Ju;Chae, Woen-Sik
    • Korean Journal of Applied Biomechanics
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    • v.20 no.2
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    • pp.175-182
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    • 2010
  • The purpose of this study was to evaluate the biomechanical effect of sports taping on the lower limb during drop landing. Twelve male university students who have no musculoskeletal disorder were recruited as the subjects. Principal strain, median frequency, vertical GRF, loading rate, angular velocity and resultant joint moment were determined for each trial. For each dependent variable, paired t-test was performed to test if significant difference existed between taped and untaped conditions(p<.05). The results showed that principal strain of the thigh and the shank in taping group were significantly less than those found in control group. These indicated that sports taping may prevent excessive mechanical strain caused by impact force during the deceleration phase. Flexion(-)-extension(+) and varus(-)-valgus(+) resultant joint moment of the knee joint in taping group were greater than corresponding value for control group. It seems that extensor muscle of the knee joint were not only supported by sports taping during knee flexion but also sports taping is effective for minimizing the possibility of injury.

A Biomimetic Artificial Neuron Matrix System Based on Carbon Nanotubes for Tactile Sensing of e-Skin (인공촉각과 피부를 위한 탄소나노튜브 기반 생체 모방형 신경 개발)

  • Kim, Jong-Min;Kim, Jin-Ho;Cha, Ju-Young;Kim, Sung-Yong;Kang, In-Pil
    • Journal of Institute of Control, Robotics and Systems
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    • v.18 no.3
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    • pp.188-192
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    • 2012
  • In this study, a carbon nanotube (CNT) flexible strain sensor was fabricated with CNT based epoxy and rubber composites for tactile sensing. The flexible strain sensor can be fabricated as a long fibrous sensor and it also may be able to measure large deformation and contact information on a structure. The long and flexible sensor can be considered to be a continuous sensor like a dendrite of a neuron in the human body and we named the sensor as a biomimetic artificial neuron. For the application of the neuron in biomimetic engineering, an ANMS (Artificial Neuron Matrix System) was developed by means of the array of the neurons with a signal processing system. Moreover, a strain positioning algorithm was also developed to find localized tactile information of the ANMS with Labview for the application of an artificial e-skin.

Biological Control Effect of Treating Avirulent Bacteriocin-Producing Strain of Pseudomonas sozanacearum Adapted to Low Temperature on Tobacco Bacterial Wilt (비병원성 Bacteriocin 생성 Pseudomonu solonueomm의 저온성 균주를 이용한 담배 세균성마름병 방제효과)

  • 이영근;손준수
    • Journal of the Korean Society of Tobacco Science
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    • v.15 no.1
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    • pp.26-33
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    • 1993
  • Effect of an avirulent bacteriocin-producing strain(ABPS) of Pseudomonas solanacearum adapted to low temperature on the control of tobacco bacterial wilt was examined under the natural field conditions. The ABPS of p. solanacearum were succeeding-cultured at gradually low temperature, 3$0^{\circ}C$ to 13$^{\circ}C$. The isolates adapted to low temperature grew faster than the wild type either in artificial media or on the tobacco rhizoplane. The control effect of one of the isolates on bacterial wilt was higher than that of the wild type when the bacterial suspension had been poured onto the tobacco rhizosphere soil on 1 day before and 15 days after transplanting to the field. It was suggested that ABPS of p. solanaceamm adapted to the low temperature, might be more effective biological control agent than the wild type.

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Analysis of stamping for the Lower control arm using Explicit code (Explicit code를 이용한 Lower control arm의 스탬핑 해석)

  • 하원필;임세영
    • Transactions of the Korean Society of Automotive Engineers
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    • v.2 no.4
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    • pp.50-58
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    • 1994
  • To examine the residual stress field resulting from stamping process for the lower control arm of a car, the explicit finite element analysis is performed for the stamping process by way of the ABAQUS Explicit. The residual stress is obtained in terms of the Von Mises stress and other parameters such as equivalent plastic strain, the change of blank thickness, the final configuration of the blank and the spring back effect are also considered. Moreover, discussed is the convergence of the explicit FEM versus the punch sped and the element discretization

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Ratcheting behavior of 90° elbow piping under seismic loading

  • Chen, Xiaohui;Huang, Kaicheng;Ye, Sheng;Fan, Yuchen;Li, Zifeng
    • Earthquakes and Structures
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    • v.17 no.5
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    • pp.489-499
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    • 2019
  • Elastic-plastic behavior of nuclear power plant elbow piping under seismic loads has been conducted in this study. Finite element analyses are performed using classical Bilinear kinematic hardening model (BKIN) and Multilinear kinematic hardening model (MKIN) as well as a nonlinear kinematic hardening model (Chaboche model). The influence of internal pressure and seismic loading on ratcheting strain of elbow pipe is studied by means of the three models. The results found that the predicted results of Chaboche model is maximum, closely followed by the predicted results of MKIN model, and the minimum is the predicted results of BKIN model. Moreover, comparisons of analysis results for each plasticity model against predicted results for a equivalent cyclic loading elbow component and for a simplified piping system seismic test are presented in the paper.

Control of Powdery Mildew of Pepper Using Culture Solutions of Chitinolytic Bacteria, Chromobacterium sp. and Lysobacter enzymogenes (키틴분해세균 Chrobacterium sp.와 Lysobacter enzymogenes의 배양액을 이용한 고추 흰가루병의 방제)

  • Seo, Chong-Chan;Jung, Hyun-Chae;Park, Seur-Kee
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.40-44
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    • 2007
  • Powdery mildew of pepper is one of the most devastating diseases which is occurring all the year under greenhouse condition. In this study, control efficacy against powdery mildew was evaluated by mixed culture solutions of two chitinolytic bacteria, Lysobacter enzymogenenes strain C-3 and Chrornobacterium sp. strain C-61, cultivated in the chitin-supplemented medium. In all experiments, white powder on the reverse side of pepper leaves perfectly disappeared 3 days after application of mixed culture solutions. However, periods required for formation of new white powder on the same sites after application (control-lasting period) were largely differed according to environmental conditions. In particular, the control-lasting period was much longer when sprayed on 6 PM than 9 AM and especially, on rainy days than sunny days. This indicates that control efficacy of culture solution may be largely affected by environmental conditions after application. The undiluted culture solution resulted in a perfect control with control value more than 95% by application of 5-day-intervals under severely diseased field and 7-day-intervals under disease-started field. A ten-fold diluted product also showed control value more than 81% by application of the same method. These results suggest that this culture solution can be practically used to control powdery mildew disease in pepper plants.

Development of a New LCF Life Prediction Model of 316L Stainless Steel at Elevated Temperature (316L 스테인리스 강의 고온 저주기 피로 수명식 개발)

  • Hong, Seong-Gu;Lee, Soon-Bok
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.3
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    • pp.521-527
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    • 2002
  • In this paper, tensile behavior and low cycle fatigue behavior of 316L stainless steel which is currently favored structural material for several high temperature components such as the liquid metal cooled fast breeder reactor (LMFBR) were investigated. Research was performed at 55$0^{\circ}C$, $600^{\circ}C$ and $650^{\circ}C$ since working temperature of 316L stainless steel in a real field is from 40$0^{\circ}C$ to $650^{\circ}C$. From tensile tests performed by strain controls with $1{\times}10^{-3}/s,\; l{\times}10^{ -4}/s \;and\; 1{\times}10/^{ -5}/ s $ strain rates at each temperature, negative strain rate response (that is, strain hardening decreases as strain rate increases) and negative temperature response were observed. Strain rate effect was relatively small compared with temperature effect. LCF tests with a constant total strain amplitude were performed by strain control with a high temperature extensometer at R.T, 55$0^{\circ}C$, $600^{\circ}C$, $650^{\circ}C$ and total strain amplitudes of 0.3%~0.8% were used and test strain rates were $1{times}10^{-2} /s,\; 1{times}10^{-3} /s\; and\; 1{times}10^{-4} /s$. A new energy based LCF life prediction model which can explain the effects of temperature, strain amplitude and strain rate on fatigue life was proposed and its excellency was verified by comparing with currently used models.