• Title/Summary/Keyword: Strain test

Search Result 4,707, Processing Time 0.03 seconds

Distribution of Toxin Genes and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Seafood in Gwangju (광주지역 유통·판매 수산물에서 분리된 장염비브리오의 독소유전자 분포 및 항생제 내성 조사)

  • Jeong, Hye Jin;Lee, Min Gyou;Lee, Hyang Hee;Seo, Si Eun;Jeong, So Hyang;Cho, Bae Sik;Seo, Jung Mi
    • Journal of Food Hygiene and Safety
    • /
    • v.37 no.2
    • /
    • pp.63-68
    • /
    • 2022
  • The purpose of this study is to investigate the distribution of toxin genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in Gwangju. A total of 335 seafood, including 163 shellfish, 97 fish, and 36 mollusk, were tested in this study. As a result, V. parahaemolyticus was detected in 123 (36.7%) of 335 seafood. The tdh gene was not detected in all strains, while the trh gene was detected in 3 strains (2.4%). According to antimicrobial susceptibility test, 116 strains (94.3%) represent resistance to ampicillin, and 1 strain (0.8%) represents resistance to trimethoprim/sulfametoxazole. However, all strains were sensitive to 9 antimicrobial agents, including amikacin, chloramphenicol, tetracycline, and more. Therefore, the risk of V. parahaemolyticus isolated from seafood in Gwangju is considered low, but continuous monitoring of V. parahaemolyticus in seafood is required.

Current Status of X-ray CT Based Non Destructive Characterization of Bentonite as an Engineered Barrier Material (공학적방벽재로서 벤토나이트 거동의 X선 단층촬영 기반 비파괴 특성화 현황)

  • Diaz, Melvin B.;Kim, Joo Yeon;Kim, Kwang Yeom;Lee, Changsoo;Kim, Jin-Seop
    • Tunnel and Underground Space
    • /
    • v.31 no.6
    • /
    • pp.400-414
    • /
    • 2021
  • Under high-level radioactive waste repository conditions, bentonite as an engineered barrier material undergoes thermal, hydrological, mechanical, and chemical processes. We report the applications of X-ray Computed Tomography (CT) imaging technique on the characterization and analysis of bentonite over the past decade to provide a reference of the utilization of this technique and the recent research trends. This overview of the X-ray CT technique applications includes the characterization of the bentonite either in pellets or powder form. X-ray imaging has provided a means to extract grain information at the microscale and identify crack networks responsible for the pellets' heterogeneity. Regarding samples of pellets-powder mixtures under hydration, X-ray CT allowed the identification and monitoring of heterogeneous zones throughout the test. Some results showed how zones with pellets only swell faster compared to others composed of pellets and powder. Moreover, the behavior of fissures between grains and bentonite matrix was observed to change under drying and hydrating conditions, tending to close during the former and open during the latter. The development of specializing software has allowed obtaining strain fields from a sequence of images. In more recent works, X-ray CT technique has served to estimate the dry density, water content, and particle displacement at different testing times. Also, when temperature was added to the hydration process of a sample, CT technology offered a way to observe localized and global density changes over time.

Structural Performance Evaluation of a Multi-span Greenhouse with Venlo-type Roof According to Bracing Installation (가새 설치에 따른 벤로형 지붕 연동온실의 구조성능 평가)

  • Shin, Hyun Ho;Choi, Man Kwon;Cho, Myeong Whan;Kim, Jin Hyun;Seo, Tae Cheol;Lee, Choung Kuen;Kim, Seung Yu
    • Journal of Bio-Environment Control
    • /
    • v.31 no.4
    • /
    • pp.438-443
    • /
    • 2022
  • In this study, the lateral loading test was performed to analyze structural performance of multi-span plastic greenhouse through full-scale experiment and numerical analysis. In order to analyze the lateral stiffness and stress, we installed 9 displacement sensors and 19 strain gauge sensors on the specimen, respectively, and load of l mm per minute was applied until the specimen failure. In the comparison between the full-scale experiment and the structural analysis results of a multi-span greenhouse with venlo-type roof according to bracing installation, there was a large difference in the lateral stiffness of the structure. By installing a brace system, the lateral stiffness measured near the side elevation of the specimen increased by up 44%. As the bracing joint used in the field did not secure sufficient rigidity, the external force could not be transmitted to the entire structure properly. Therefore, it is necessary to establish a bracing construction method and design standards in order for a greenhouse to which bracing applied to have sufficient performance.

Rapid Cell Death Phenotype of Streptococcus mutans under Prolonged Growth Conditions (장시간 생장 조건에서 Streptococcus mutans의 급격한 세포사 표현형 분석)

  • Kim, Jeong Nam
    • Journal of Life Science
    • /
    • v.31 no.12
    • /
    • pp.1072-1078
    • /
    • 2021
  • The oral pathogen Streptococcus mutans is considered a major causative agent of dental caries in humans. The use of dental hygiene products, including toothpaste and mouthwash, is used for caries control. However, food intake can lead to the recurrence of oral microorganisms. This study aimed to explore why this bacterium dies so quickly during prolonged incubation and to assess whether this growth characteristic is closely associated with the secretion of metabolic products. Notably, the number of live S. mutans cells rapidly declined after 24 hr during the entire period tested, whereas the number of Escherichia coli cells, an indicator strain, remained steady over the same period. To test whether the S. mutans supernatants contained possible signals that accelerated the death of neighbor cells, we obtained the individual supernatants at the above time points. Following pH neutralization, the cells in which the supernatant was supplemented with glucose grew well. However, pH adjustment alone could not fully recover cell growth in conditions in which the supernatant was supplemented, with or without glucose. These phenotypes of S. mutans may be associated with signaling, not only resulting from nutrient depletion. The findings on the survival phenotype of S. mutans provide new insights into cell-cell communication in the biology of this bacterium.

Strength Characteristics of 3D Printed Composite Materials According to Lamination Patterns (적층 패턴에 따른 3D 프린팅 복합재료의 강도특성)

  • Seo, Eun-A;Lee, Ho-Jae;Yang, Keun-Hyeok
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.25 no.6
    • /
    • pp.193-198
    • /
    • 2021
  • In this study, the rheological characteristics and of 3D printing composite materials and the compressive strength characteristics according to the lamination patterns were evaluated. As a result of rheology test, rapid material change was observed after 60 minutes of extrusion, yielding stress 1.4 times higher than immediately after mixing, and plastic viscosity was 14.94-25.62% lower. The compressive strength of the specimens manufactured in the mold and the laminated specimens were compared, and the lamination pattern of the laminated specimens were 0°, 45°, and 90° as variables. The compressive strength of the mold casting specimen and the laminated specimen from 1 to 28 days of age showed similar performance regardless of the lamination pattern. In particular, at the age of 28 days, the modulus of elasticity, maximum compressive strength, and strain at maximum stress of all specimens were almost the same. In order to analyze the interface of the laminated specimens, X-ray CT analysis of the specimen whose compressive strength were measured was performed. Through CT analysis, it was confirmed that cracks did not occur at the lamination interface, which can be judged that the interface in the laminated specimen behaved in an integrated manner.

Field Test for Estimation of Acting Force on the Drum Cutter Attachment (드럼커터 어태치먼트의 작용력에 대한 현장시험)

  • Soon-Wook, Choi;Chulho, Lee;Tae-Ho, Kang;Soo-Ho, Chang
    • Tunnel and Underground Space
    • /
    • v.32 no.6
    • /
    • pp.373-385
    • /
    • 2022
  • The drum cutter, which is used in the form of an attachment of a excavator, is very useful in that it can be used in connection with a excavator that can perform various tasks in the field. This study estimated the load and torque acting on the drum cutter attachment by measuring the hydraulic pressure and strain that appear during excavation on the exposed rock slope using the drum cutter installed in the excavator. Working conditions such as the operation angle between the boom and arm of the excavator were divided into eight working modes. And as a result of analyzing the variations in hydraulic pressure and action force according to the working mode, it was confirmed that the hydraulic pressure and flow rate can be driven without any problems within the range considered in the manufacturing specifications of the drum cutter. The average load and torque acting on the drum cutter were within the range of the manufacturing specifications, but the maximum load was up to four times the specification. Because sumping was not properly performed due to the high ground strength and the ground included discontinuous surfaces in some locations, no trend of load and torque was found depending on the angle between the boom and arm of the excavator. However, it is believed that this result can be used to determine the range of loads and torques that appear on the drum cutter when excavating a high-intensity rock.

Comparison of Conventional Culture Method, Enzyme Immune Method, and PCR for the Rapid Detection of Salmonella spp. in Pet Food (반려동물 사료의 Salmonella spp. 신속검출을 위한 증균배양법, 면역학적 검출법 및 종 특이 프라이머를 이용한 PCR 방법 비교)

  • Yun, Hyejeong;Cha, Sun Ho;Lee, Seung-Hwa;Jeong, Min-Hee;Na, Tae-Woong;Kim, Haejin;Cho, Hyunjeong;Hong, Seong-Hee
    • Journal of Food Hygiene and Safety
    • /
    • v.37 no.1
    • /
    • pp.15-20
    • /
    • 2022
  • The purpose of this study was to compare the conventional culture method, enzyme immune method and the PCR method using species-specific primer in the analysis on the Salmonella spp. found in domestically distributed pet foods. For the study, Salmonella spp. were detected from 175 samples. From the conventional culture method and the PCR method, two samples (jerky and corn gluten) were determined as positive. Also, from the enzyme immune method, one sample (corn gluten) was test-positive. The study revealed that application of the PCR method with species-specific primer allows better distinguishment between the species of the strain collected from the samples than the conventional culture method and/or the enzyme immune method.

Damping Performance Evaluation of Hysteretic Strip Damper with Curvature (곡률이 있는 이력형 스트립 댐퍼의 감쇠 성능 평가)

  • Jae Won Lee;Dong Baek Kim;Yong Gon Kim;Jeong Ho Choi;Jong Hoon Kim
    • Journal of the Society of Disaster Information
    • /
    • v.19 no.3
    • /
    • pp.572-581
    • /
    • 2023
  • Purpose: The purpose of this study is to improve the irregularity of the stress-strain curve and to ensure accuracy when calculating the damping effect by preventing members from moving in the off-plane direction due to eccentricity when loads are applied. Method: The specifications of the steel strips used in this study are the same, but the curvature of the strips to constitute each damper is different. Each steel strip with different curvature was arranged in an triangle, three dampers with different curvature were made, and repeated load tests were conducted, and the amount of energy dissipation was calculated to measure the performance of the damper. Result: The amount of energy dissipation significantly decreases compared to the case where there is no initial curvature, and the change in the test energy dissipation amount according to the size of the curvature is not large, and the presence or absence of the hyperbolic rate is considered an important variable. Conclusion: The period is about 78.7% longer from T=0.3 to T=0.536sec, and the response spectrum acceleration is reduced from Sa=0.54g to Sa=0.229g, so the damping effect of the damper is sufficient.

Effect of Water on the Lightweight Air-Mixed Soil Containing Silt Used for Road Embankment (도로성토체로 사용된 실트질 계열의 경랑기포혼합토에 대한 물의 영향)

  • Hwang, Joong-Ho;Ahn, Young-Kyun;Kim, Tae-Hyung
    • Journal of the Korean Geotechnical Society
    • /
    • v.26 no.2
    • /
    • pp.23-32
    • /
    • 2010
  • This study was especially conducted to find out the characteristics of the lightweight air-mixed soil (slurry density 10 kN/$m^3$) containing silt related to water. Compression strength, permeability, and capillary height of the lightweight air-mixed soil were studied, and also to support these studies, the structure of that soil was analyzed in detail. Air bubbles of various sizes are inside the lightweight air-mixed soil, and its distribution in a location is almost constant. A numerous tiny pores are inside the air bubbles so that the lightweight air-mixed soil can be saturated with water. Porosity is also estimated through the image analysis. Peak strength of the lightweight air-mixed soil is not dependent on water, but behavior of stress-strain is affected by the water. Permeability is about $4.857{\times}10^{-6}cm/sec$, which is a little bit higher than the clay's permeability. Capillary rise occurs rapidly at the beginning of the test until the lapse of 100 minutes and then its increase rate becomes slow. The capillary rise causes the increase of the density of the lightweight air-mixed soil, and thus it is required to pay attention to this phenomenon during structure design and maintenance of the lightweight air-mixed soil.

Development of Composite Geo-Material for Recycling Dredged Soil and Bottom Ash (준설토와 Bottom Ash 재활용을 위한 복합지반재료 개발)

  • Kim, Yun-Tae;Han, Woo-Jong;Jung, Du-Hwoe
    • Journal of the Korean Geotechnical Society
    • /
    • v.23 no.11
    • /
    • pp.77-85
    • /
    • 2007
  • This paper investigates the mechanical characteristics of composite geo-material which was developed to reuse both dredged soils and bottom ash. The composite geo-material used in this experiment consists of dredged soil taken from the construction site of Busan New Port, cement, air foam and bottom ash. Bottom ash is a by-product generated at the Samcheonpo thermal power plant. Several series of laboratory tests were performed to investigate behavior characteristics of composite gee-material, in particular the reinforcing effect by mixing bottom ash. The experimental results of composite geo-material indicated that the stress-strain relationship and the unconfined compressive strength are strongly influenced by mixing conditions. Especially it was observed that the compressive strength of composite geo-material increased with an increase in bottom ash content due to reinforcing effect by the bottom ash. Compressive strength of composite geo-material increased with the increase in curing time. The 28-day strength of composite geo-material is $1.7{\sim}1.8$ times higher than the 7-day strength. The moist unit weight strongly depended on air-foam content as well as bottom ash content added to the composite goo-material. In composite geo-material, secant modulus ($E_{50}$) also increased as its compressive strength increased due to the inclusion of bottom ash.