• Journal of Life Science
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• v.31 no.1
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• pp.28-36
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• 2021

Edwardsiella piscicida is a significant cause of hemorrhagic septicemia in fish and gastrointestinal infections in humans. Survival bacteria require specialized mechanisms to adapt to environmental fluctuations. Hence, to understand the mechanism through which E. piscicida senses and responds to environmental osmolarity changes, we determined the protein expression profile and physiological properties under various salinity conditions in this study. The OmpR protein is a part of the Env-ZOmpR two-component system that has been implicated in sensing salt stress in bacteria. However, the physiological role played by this protein in E. piscicida remains to be elucidated. Therefore, in this work, the function of the OmpR protein in response to salt stress was investigated. Phenotypic analysis revealed that, in the mutant, three of the biochemical phenotypes were different from the wild type, including, citrate utilization, hydrogen sulfide, and indole production. Introduction of the plasmid containing the entire ompR gene to the mutant strain returned it to its parental phenotype. The retarded growth rate also partially recovered. Furthermore, in our studies, OmpR was not found to be related to cell motility. Taken together, our results from the mutational analysis, the growth assay, MALDI-TOF MS, qRT-PCR, and the phenotype studies suggest that the OmpR of E. piscicida is implicated in osmoregulation, growth, expression of porins (ETAE_1826), virulence-related genes (EseC, EseD and EvpC), and certain genes of unknown function (ETAE_1540 and ETAE_2706).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.51-57
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• 2021

The seawater cooling system of naval ships is installed to remove the toxic substances generated by CBR (Chemical, Biological, and Radiological) warfare and reduce the infrared signature of naval ships from outside the hull. The dispersion range of the nozzle is determined according to the injection pressure of seawater and the nozzle type. Therefore, it is necessary to select the appropriate injection pressure and design the optimal nozzles to increase the seawater dispersion area and maximize the efficiency of the cooling system. In this study, the applying feasibility of 3D printing technology to produce an injection nozzle for the seawater cooling system was examined. To this end, the extruded plastic specimens were fabricated by 3D printing, and the physical properties of the specimens were estimated through tensile testing. After this, the strain and stress of the nozzle as a function of the pressure were simulated by applying the estimated results to the finite element analysis. The finite element analysis results showed that the nozzle remained within the elastic range at the optimal pressure. The nozzle was estimated to be structurally stable, and the possibility of this study was confirmed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.2
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• pp.414-421
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• 2022

Subsea oil and gas exploration is increasingly moving into deeper water depths, and typically, subsea pipelines operate under high pressure and temperature conditions. Owing to the difference in these components, the axial force in the pipe is accumulated. When a pipeline is operated at a high internal pressure and temperature, it will attempt to expand and contract for differential temperature changes. Typically, the line is not free to move because of the plane strain constraints in the longitudinal direction and soil friction effects. For a positive differential temperature, it will be subjected to an axial compressive load, and when this load reaches a certain critical value, the pipe may experience vertical (upheaval buckling) or lateral (snaking buckling) movements that can jeopardize the structural integrity of the pipeline. In these circumstances, the pipeline behavior should be evaluated to ensure the pipeline structural integrity during operation in those demanding loading conditions. Performing this analysis, the correct mitigation measures for thermal buckling can be considered either by accepting bar buckling but preventing the development of excessive bending moment or by preventing any occurrence of bending.

• Journal of the Korean Geotechnical Society
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• v.37 no.11
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• pp.83-92
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• 2021

In this study, effective porosity measurement, electron microscope (SEM) observation, X-ray diffraction analysis (XRD), slaking, swelling, and unconfined compression strength according to water immersion were analyzed to evaluate the properties of mudstone with high porosity in Pohang. As a result of the test for 16 square samples (5 cm), the effective porosity was 14.67% on average, higher than porosity of general mudstone, and electron microscope observation confirmed that the porosity was actually high. As a result of X-ray diffraction analysis, the swelling clay mineral content was 2.3~4.1%, which was lower than the results of previous studies in Pohang. The slake durability index was 37.73~87.73%, showing low to medium durability, which was lower than the results of previous studies. It was confirmed that the swelling property rapidly expanded to 1.79~1.82% of maximum swelling strain in the major axis direction for 30 minutes. As the properties of decreasing the unconfined compression strength according to water immersion, the samples rapidly weathered after 10 minutes of water immersion, and the strength decreased. It was confirmed that the results of previous studies related to mudstone in Pohang were different. This is judged to be due to the high porosity of mudstone in study.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.845-855
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• 2021

In this study, Camellia sinensis Leaf cultivated in Boseong was extracted by vacuum distillation and hydrothermal extraction methods, and we performed in vitro experiments such as antioxidant, anti-wrinkle, whitening, and antibacterial activity test and HPLC analysis. Wrinkle improvement (elastase inhibition assay) and whitening effect (mushroom tyrosinase inhibition assay) of Boseong vacuum distilled extract have excellent results and IC50 values of vacuum distillation extract was measured at a lower concentration than that of the hydrothermal extract. The antioxidant activity of the Boseong hydrothermal extract was excellently measured in DPPH radical and ABTS+ radical scavenging activity, SOD-like activity, and total polyphenol content. In the antibacterial experiment, the Boseong hydrothermal extract formed a clear zone for the Staphylococcus aureus and Cutibacterium acnes strain. As a result of HPLC analysis, EGCG and caffeine in the hydrothermal extract were 40.29 mg/g, 9.94 mg/g respectively, and caffeine in the vacuum distilled extract was 3.40 mg/g. Therefore, we examined that Boseong Camellia sinensis vacuum distilled extract has excellent anti-wrinkle and whitening effect, and its hydrothermal extract has good antioxidant and antibacterial efficacy.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.438-443
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• 2022

In this study, the lateral loading test was performed to analyze structural performance of multi-span plastic greenhouse through full-scale experiment and numerical analysis. In order to analyze the lateral stiffness and stress, we installed 9 displacement sensors and 19 strain gauge sensors on the specimen, respectively, and load of l mm per minute was applied until the specimen failure. In the comparison between the full-scale experiment and the structural analysis results of a multi-span greenhouse with venlo-type roof according to bracing installation, there was a large difference in the lateral stiffness of the structure. By installing a brace system, the lateral stiffness measured near the side elevation of the specimen increased by up 44%. As the bracing joint used in the field did not secure sufficient rigidity, the external force could not be transmitted to the entire structure properly. Therefore, it is necessary to establish a bracing construction method and design standards in order for a greenhouse to which bracing applied to have sufficient performance.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.6
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• pp.193-198
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• 2021

In this study, the rheological characteristics and of 3D printing composite materials and the compressive strength characteristics according to the lamination patterns were evaluated. As a result of rheology test, rapid material change was observed after 60 minutes of extrusion, yielding stress 1.4 times higher than immediately after mixing, and plastic viscosity was 14.94-25.62% lower. The compressive strength of the specimens manufactured in the mold and the laminated specimens were compared, and the lamination pattern of the laminated specimens were 0°, 45°, and 90° as variables. The compressive strength of the mold casting specimen and the laminated specimen from 1 to 28 days of age showed similar performance regardless of the lamination pattern. In particular, at the age of 28 days, the modulus of elasticity, maximum compressive strength, and strain at maximum stress of all specimens were almost the same. In order to analyze the interface of the laminated specimens, X-ray CT analysis of the specimen whose compressive strength were measured was performed. Through CT analysis, it was confirmed that cracks did not occur at the lamination interface, which can be judged that the interface in the laminated specimen behaved in an integrated manner.

• Journal of the Korean Institute of Gas
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• v.26 no.4
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• pp.58-63
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• 2022

With the emergence of environmental problems caused by fine dust worldwide, LNG, which is cheaper and less pollution than diesel, is attracting attention as the next generation energy of automobiles and is expanding its supply. However, it is difficult to operate smoothly due to the lack of infrastructure for LNG charging stations in Korea and the limited size of containers that can be installed according to regulations. In Korea, research and development on the contents of containers for the smooth operation of natural gas vehicles are underway, but there is a problem that the container directly receives the impact of the vehicle collision and explodes, causing a major disaster. Therefore, in this study, the safety of the container was verified by deriving the strain and stress values through ANSYS Explicit Dynamics analysis. As a result, a maximum stress of 565.37MPa occurred in the container, and it is expected that plastic deformation will occur as it exceeds the yield stress of STS304 used as a material for the container, which is beyond 505MPa. When an impact caused by a collision between a vehicle and a container is applied, it is considered necessary to design a support or reinforcement because the container may be damaged or defective.

• The Journal of Engineering Geology
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• v.32 no.4
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• pp.597-610
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• 2022

Knowledge of the failure behavior of friction materials considering their intermediate principal stress is related to an understanding of situations where these materials might be used: for example, the stability of deep-seated boreholes and fault slip analysis. This study designed equipment for physically implementing true triaxial compression and used it to assess specimens of plaster, a friction material. The material's mechanical behaviors are discussed based on the results. The applicability of the 3D failure criteria are also reviewed. The tested specimens were molded cuboids of width, length, and height 52, 52, and 104 mm, respectively. A total of 24 true triaxial compression tests were performed under various combinations of 𝜎₃ and 𝜎₂ conditions. Conventional uniaxial and triaxial compression tests were employed to estimate the mechanical properties of the plaster for use as parameters for 3D failure criteria. Examining the stress-strain relations of the plaster materials showed that a large difference between the intermediate principal stress and the minimum principal stress indicated strong brittle behavior. The mechanical behavior of the plaster used here reflects the change of intermediate principal stress. Nonlinear multiple regression analysis on the test data in the principal space showed that the modified Wiebols-Cook failure criterion and the modified Lade failure criterion were the most suitable 3D failure criteria for the tested plaster.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.685-691
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• 2022

This study was based on the steel composite concrete box structure (Transfer girder) which was installed to support the skyscrapers directly above the subway line. In this study, it was analytically proved that the cause of cracks on the steel composite concrete box structure were the shrinkage cracks by comparing the results of crack investigation and numerical analysis. As the results, it was found that the internal temperature difference between concrete and steel members occurred according to the shape of the steel frame embedded in concrete, the location of vertical stiffener, and the closed section area. The narrower spacing of vertical stiffener was occurred the internal temperature concentration of the structure and the temperature difference increased. And the location of higher thermal strain and temperature were similar to the location of actual cracks by the visual inspection. Therefore, the internal temperature concentration parts were formed according to the presence and spacing of the vertical stiffeners and the inspection passage in the central part of the structure. The shrinkage cracks were occurred by the restrained of temperature expansion and contraction of the concrete. As the results of this study, it was important to separate and manage the non-structural cracks caused by shrinkage and the structural cracks in the maintenance of serviced steel-composite concrete structures.