• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.315-320
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• 1996

The role of glyoxylate bypass in a lysine-producing Corynebacterium lactofermentum strain was analyzed. Unlike the wild type, the strain expressed enzymes of glyoxylate bypass during growth in the fermentation broth containing glucose as the carbon source. To evaluate the importance of glyoxylate bypass in the strain, we disrupted chromosomal aceA by using a cloned fragment of the gene. Site-specific disruption of aceA which codes for the isocitrate lyase, the first enzyme of the bypass, was confirmed by Southern blot analysis. The aceA mutant strain completely lost isocitrate lyase activity and ability to grow in a minimal medium containing acetate as the sole carbon source. The mutant strain was similar to its parental strain in growth characteristics and produced comparable amounts of lysine in shake flasks containing glucose as the carbon source. The amount of oxaloacetate accumulated in the fermentation medium was similar for both strains, suggesting that expression of glyoxylate bypass does not necessarily lead to the increase in intracellular oxaloacetate. These data clearly demonstrate that glyoxylate bypass does not function as one of the routes of carbon supply for lysine production in the strain. It appears that the leakiness of the glyoxylate bypass in the strain might be the result of a secondary mutation which arose during previous strain development by random mutagenesis.

• Transactions of Materials Processing
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• v.4 no.2
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• pp.169-179
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• 1995

Static restoration mechanism during hot interrupted deformation of Cu-Zn alloy was studied in the temperature range from $550^{\circ}C$ to $750^{\circ}C$ and at a constant strain rate of 0.1/sec. At a given temperature, the hot interrupted deformations were performed with variation of interrupted time $t_i$ form 1 to 50 sec and of interrupted strain ${\varepsilon}_i$ from 0.15 to 0.90. From the analysis of the values of the critical strain of ${\varepsilon}_c$ for tje initiation of dynamic recrystallization and the peak strain of${\varepsilon}_p$, the relationship ${\varepsilon}_c{\fallingdotseq}0.7{\varepsilon}_p$ was obtained. It was clarified that the softening of the interrupted deformation was mainly the static recrystallization and the fractional softening(FS) which was over 30% mostly confirmed this result. The fractional softening of the interrupted time $t_i$ especially and pre-strain. The FS increased with increasing strain rate, interrupted time and pre-strain. The change of microstructures after hot deformation could be predicted by the FS. when the FS was 30~100%, static recrystallization was happened and grain growth was observed at the condition which was $750^{\circ}C$ deformation temperature, 0.45 prestrain and this condition's FS value was over 100%.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2000.10a
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• pp.33-36
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• 2000

The dynamic recrystallization (DRX) of medium carbon steels (SCM 440 and POSMA45) was studied with torsion test in the temperature range of $900-1100^{\circ}C$ and the strain rate range of $5.0x10^{-2}\;-\;5.0x10^0/sec$. To establish the quantitative equations for DRX, the evolution of flow stress curve with strain was analyzed. The critical strain (${\varepsilon}_c$) and strain for maximum softening rate ( ${\varepsilon}^{*}$) could be confirmed by the analysis of work hardening rate ($d{\sigma}/d{\varepsilon}\;=\; \theta$). The volume fraction of dynamic recrystallization ($X_{DRX}$) as a function of processing variables, such as strain rate ( $\dot{\varepsilon}$ ), temperature (T), and strain ( $\varepsilon$ ) were established using the ${\varepsilon}_c$ and ${\varepsilon}^{*}$. For the exact prediction, the ${\varepsilon}_c$, ${\varepsilon}^{*}$ and Avrami' exponent (m') were quantitatively expressed by dimensionless parameter, Z/A respectively. The transformation-effective strain-temperature curve for DRX could be composed. It was found that the calculated results were agreed with the experimental data for the steels at any deformation conditions.

• Structural Engineering and Mechanics
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• v.54 no.2
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• pp.199-219
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• 2015

This paper presents the non-destructive evaluation of a high-speed railway bridge using train-induced strain responses. Based on the train-track-bridge interaction analysis, the strain responses of a high-speed railway bridge under moving trains with different operation status could be calculated. The train induced strain responses could be divided into two parts: the force vibration stage and the free vibration stage. The strain-displacement relationship is analysed and used for deriving critical displacements from theoretical stain measurements at a forced vibration stage. The derived displacements would be suitable for the condition assessment of the bridge through design specifications defined indexes and would show certain limits to the practical application. Thus, the damage identification of high-speed railways, such as the stiffness degradation location, needs to be done by comparing the measured strain response under moving trains in different states because the vehicle types of high-speed railway are relatively clear and definite. The monitored strain responses at the free vibration stage, after trains pass through the bridge, would be used for identifying the strain modes. The relationship between and the degradation degree and the strain mode shapes shows certain rules for the widely used simply supported beam bridges. The numerical simulation proves simple and effective for the proposed method to locate and quantify the stiffness degradation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.6
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• pp.2284-2291
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• 2010

In this paper, we investigate the vibration analysis of plates, using an 8-node shell element that accounts for the transverse shear strains and rotary inertia. The forced vibration analysis of plates subjected to arbitrary loading is investigated. In order to overcome membrane and shear locking phenomena, the assumed natural strain method is used. To improve an 8-node shell element for forced vibration analysis, the new combination of sampling points for assumed natural strain method was applied. The refined first-order shear deformation theory based on Reissner-Mindlin theory which allows the shear deformation without shear correction factor and rotary inertia effect to be considered is adopted for development of 8-node assumed strain shell element. In order to validate the finite element numerical solutions, the reference solutions of plates are presented. Results of the present theory show good agreement with the reference solution. In addition the effect of damping is investigated on the forced vibration analysis of plates.

• 한국공간정보시스템학회:학술대회논문집
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• 2004.05a
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• pp.230-237
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• 2004

In this study, equation of finite element is formulated to analyze relations of large deformation-small deformation considering geometrical nonlinear for membrane structure. Total Lagrangian Formulation(TLF) is introduced to formulate theory and equation of motion considering Triangular Constant Strain(TCS) element in finite, element analysis is formulated. Finite element program is made by equation of motion considering TLF. This study analyzed a variety of examples, so compared with the past results.

• Applied Microscopy
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• v.45 no.2
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• pp.101-105
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• 2015

Numerous digital image analysis techniques have been developed with regard to transmission electron microscopy (TEM) with the help of programming. DigitalMicrograph (DM, Gatan Inc., USA), which is installed on most TEMs as operational software, includes a script language to develop customized software for image analysis. Based on the DM script language, this work provides a script source listing for quantitative strain measurements based on a geometric phase analysis.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2002.10a
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• pp.69-74
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• 2002

This study is object to structural analysis of high pressure injection nozzle. The finite element model was developed to compute the stress, strain for high pressure injection nozzle. For structural analysis using result from FEM code. This structural analysis results, many variables such as internal pressure, boundary condition, constraint condition and load condition are considered.

• Transactions of Materials Processing
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• v.5 no.2
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• pp.156-164
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• 1996

To investigate the behavior of plastic deformation in axisymmetric extrusion through square dies, experimental works with the plasticine as a model material are carried out at the room temperature. Some mechanical properties of the model material are determined by compression and ring compression tests. Visioplasticity method using expermental grid distortion in extrusion is introduced to analyze the plastic flow strain rate and strain distribution. In spite of severe deformation during the extrusion through square die the visioplasticity method shows good results for the distribution of effective strain rate and effective strain.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.12 no.3
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• pp.457-464
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• 1999

The object of this study is form finding, stress-strain analysis and cutting pattern analysis of membrane structures under the following assumptions : (1) material is linearly elastic (2) stress state is plane stress. The cable and membrane structures undergo large deformation because of its highly flexibility, therefore, we must take account of its geometric nonlinearity. The analysis procedure is consisted of three steps considering geometric nonlinearity unlike any other structures. First step is the form finding analysis to determine the initial equilibrium shape. Second step is the stress-strain analysis to investigate the behaviors of structures under various external loads. Once a stationary shape has been fount a cutting pattern based on the form finding analysis may be generated for manufacturing procedure. In this paper, form finding, stress-strain analysis and cutting pattern analysis is carried out for applying to Seoguipo worldcup soccer stadium roof structures and optimal cutting pattern analysis technique is proposed.