• Journal of Life Science
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• v.13 no.4
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• pp.421-427
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• 2003

A natural habit at bacterium, Enterobacter intermedious KH410 was isolated from freshwater plant root and identified. Adsorption of heavy metals such as lead, cadmium, and copper by this strain was examined. The minimal inhibitory concentrations(MIC) for each metal were 1.78 mM for lead, 0.17 mM for cadmium and 1.39 mM for lopper, respectively. Maximum production of dried cell was 2.56 g/$\ell$ in LB medium containing 0.5% NaCl, 1% yeast extract and 1% of lactose. Optimal conditions for adsorption were 0.6 dry g-biomass, at pH 4.0 and the temperature of $20^{\circ}C$. Adsorption equilibrium reached maximum after 30 min in 400 mg/$\ell$ metal solution. The adsorption capacity (K) of copper was 1.5 times higher than that of cadmium and lead was 1.1 times higher than that of cadmium. from the results obtained in this study, Freundlich adsorption model was applicable for all metals. Adsorption strength (1/n) of heavy metal ions were in the order of cadmium>copper>lead. The adsorption of dried cell for lead, cadmium, and copper was 56.2, 58.0, 55.8 mg/g-biomass, respectively. Pretreatment to increase ion strength was the most effective with 0.1 M NaOH whereas slight difference was found both KOH and $CaCl_2$ upon same concentration. Effective desorption was induced by 0.1 M EDTA for lead and 0.1 M $HNO_2$ for cadmium and copper.

• The Korean Journal of Food And Nutrition
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• v.27 no.1
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• pp.43-49
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• 2014

Panax ginseng C.A. Meyer has been used as a traditional medicinal ingredient and the ginseng flower-buds also proved to have good medicinal properties. In this study, in order to enhance immune activities of ginseng flower-bud, the ginseng flower-bud extract was being fermented by Bacillus subtilis KCTC 1022 (BS), Lactobacillus plantarum KCTC3 and Saccharomyces cerevisiae strain CHY1011 (SC). Mice were orally administered daily for two weeks at two different concentrations (100 and 200 mg/kg B.W.). Treatment samples were water extracts of ginseng flower-buds (FD), water extracts of fermented ginseng flower-buds (FM) and controls for saline solution. Cytokine production (IL-6, TNF-${\alpha}$) either stimulated with LPS or not stimulated with LPS was detected by the ELISA assay when using the cytokine kit. Cytokine was statistically increased at supplemented groups with LPS in both the 100 and the 200 mg/kg B.W. and treatment with FM significantly decreased the LPS-induced TNF-${\alpha}$ and IL-6 production more than the treatment with FD. The results of this study may suggest that supplementation with FM increases the immune function by regulating cytokine production capacity for activated macrophages.

• International Journal of High-Rise Buildings
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• v.10 no.4
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• pp.335-344
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• 2021

In structural engineering practice, understanding the performance of composite columns under extreme loading conditions such as high-rise bulding, long span and heavy loads is essential to accuratly predicting of material responses under severe loads such as fires or earthquakes. Hitherto, the combined effect of partial axial loads and subsequent elevated temperatures on the performance of hollow steel column filled fly ash concrete have not been widely investigated. Comprehensive test was carried out to investigate the effect of elevated temperatures on partial axially loaded square hollow steel column filled fly ash concrete as reported in this paper. Four batches of hollow steel column filled fly ash concrete ( 30 percent replacement of fly ash), (HySC) and normal concrete (CFHS) were subjected to four different load levels, n_f of 20%, 30%, 40% and 50% based on ultimate column strength. Subsequently, all batches of the partially damage composite columns were exposed to transient elevated temperature up to 250℃, 450℃ and 650℃ for one hour. The overall stress - strain relationship for both types of composited columns with different concrete fillers were presented for each different partial load levels and elevated temperature exposure. Results show that CFHS column has better performance than HySC at ambient temperature with 1.03 relative difference. However, the residual ultimate compressive strength of HySC subjected to partial axial load and elevated temperature exposure present an improvement compared to CFHS column with percentage difference in range 1.9% to 18.3%. Most of HySC and CFHS column specimens failed due to local buckling at the top and middle section of the column caused by concrete crushing. The columns failed due to global buckling after prolong compression load. After the compression load was lengthened, the columns were found to fail due to global buckling except for HySC02.

• Journal of the Korean Geotechnical Society
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• v.35 no.7
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• pp.15-27
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• 2019

The purpose of this study is to investigate the mechanism about damages occurring at quay wall and backfill in Youngilman Port during Pohang earthquake (M5.4) on November 15, 2017. In the field investigation, the horizontal displacement of the caisson occurred between 5 cm and 15 cm, and the settlement at backfill occurred higher than 10 cm. 2D-effective Stress Analysis was performed to clarify the mechanism for the damage. The input earthquake motion used acceleration ($3.25m/s^2$) measured at bedrock of Pohang habor. Based on a numerical analysis, it was found that the effective stress decreased due to the increase of excess pore pressure in the backfill ground and the horizontal displacement of the caisson occurred by about 14 cm, and the settlement occurred by about 3 cm. In backfill, the settlements occurred between 6 cm and 9 cm. This is similar to field investigation results. Also, it was found that the backfill soil was close to the Mohr-Coulomb failure line due to the cyclic loading from the effective stress path and the stress-strain behavior. It may be related to decreasing of bearing capacity induced by the reduction of effective stress caused by the increase of the excess pore water pressure.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.116-122
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• 2021

This study quantitatively evaluated the performance improvement of a circular reinforced concrete pier under dynamic load with strengthening using a steel plate. Various three-dimensional elements were applied using the finite element program ABAQUS. The analytical parameters included the ratios of the steel cover length to the pier's total height and the ratios of the steel cover thickness to the pier diameter for inelastic-nonlinear analysis. The lower part of the pier had fixed boundary conditions, and lateral repetitive loads were applied at the top of the pier. The pier was investigated to evaluate the dynamic performance based on the load-displacement curve, stress-strain curve, ductility, energy absorption capability, and energy ratio. The yield and ultimate loads of piers with steel covers increased by 3.76 times, and the energy absorption capability increased by 4 times due to the confinement effects caused by the steel plate. A plastic hinge part of the column with a steel plate improved the ductility, and the thicker the steel plate was, the greater the energy absorption capacity. This study shows that the reinforced pier should be improved in terms of the seismic performance.

• Korean Journal of Poultry Science
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• v.48 no.4
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• pp.217-225
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• 2021

This study compares the physicochemical characteristics and fatty acid composition of three Korean native chickens and broilers. Ten whole raw broiler chickens and ten each from the three Korean native chickens (KNCs), Hanhyup 3 (HH3), Woormatdak 1 (WRMD1), and Woormatdak 2 (WRMD2), were purchased from the meat market. Their breast and thigh meat were used as samples. The proximate composition, pH, color, water-holding capacity (WHC), shear force, collagen content, and fatty acid composition were determined. In breast meat, the moisture content of HH3 (74.94%) and WRMD1 (74.74%) was lower than that of the broilers (77.1%, P<0.05). No significant difference was found in crude protein, lipids, and ash contents. The crude fat from thigh meat from HH3 and WRMD2 was lower than that of broilers (P<0.05). The redness of WRMD1 was the highest in both breast and thigh meat (P<0.05). The WHC of the breast meat of WRMD1 was lower than that of HH3 and WRMD2. In thigh meat, the WHC of the broilers was significantly higher than that of the KNCs. In breast meat, the shear force of WRMD2 was significantly lower than that of the broilers, HH3, and WRMD1, while no significant difference was found in thigh meat. The collagen content and arachidonic acid levels of the KNCs were significantly higher than those of the broilers for breast and thigh meats. No significant differences were observed among the KNCs. This result can be used to improve the quality of KNC but further studies on the bioactive compounds, taste, and volatile compounds of KNCs are required.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.6
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• pp.50-59
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• 2021

Currently, the response correction factor is calculated by comparing the response measured by the load test on a bridge with the response analyzed in the initial analytical model. Then the load rating and the load carrying capacity are evaluated. However, the response correction factor gives a value that fluctuates depending on the measurement location and load condition. In particular, when the initial analytical model is not suitable for representing the behavior of a bridge, the range of variation is large and the analysis response by the calibrated model may give a result that is different from the measured response. In this study, a pseudo-static load test was applied to obtain static response with dynamic components removed under various load conditions of a vehicle moving at a low speed. Static response was measured on two similar PSC-I girder bridges, and the response correction factors for displacement and strain were calculated for each of the two bridges. When the initial analysis model was not properly set up, it is verified that the response of the analytical model corrected by the average response correction factor does not fall within the margin of error with the measured response.

• Structural Engineering and Mechanics
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• v.90 no.6
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• pp.611-633
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• 2024

This study intends to investigate the response of multi-cell (MC) beams to flexural loads in which the primary reinforcement is composed of both metallic and non-metallic materials. "Multi-cell" describes beam sections with multiple longitudinal voids separated by thin webs. Seven reinforced concrete MC beams measuring 300×200×1800 mm were tested under flexural loadings until failure. Two series of beams are formed, depending on the type of main reinforcement that is being used. A control RC beam with no openings and six MC beams are found in these two series. Series one and two are reinforced with metallic and non-metallic main reinforcement, respectively, in order to maintain a constant reinforcement ratio. The first crack, ultimate load, deflection, ductility index, energy absorption, strain characteristics, crack pattern, and failure mode were among the structural parameters of the beams under investigation that were documented. The primary variables that vary are the kind of reinforcing materials that are utilized, as well as the kind and quantity of mesh layers. The outcomes of this study that looked at the experimental and numerical performance of ferrocement reinforced concrete MC beams are presented in this article. Nonlinear finite element analysis (NLFEA) was performed with ANSYS-16.0 software to demonstrate the behavior of composite MC beams with holes. A parametric study is also carried out to investigate the factors, such as opening size, that can most strongly affect the mechanical behavior of the suggested model. The experimental and numerical results obtained demonstrate that the FE simulations generated an acceptable degree of experimental value estimation. It's also important to demonstrate that, when compared to the control beam, the MC beam reinforced with geogrid mesh (MCGB) decreases its strength capacity by a maximum of 73.33%. In contrast, the minimum strength reduction value of 16.71% is observed in the MC beams reinforced with carbon reinforcing bars (MCCR). The findings of the experiments on MC beams with openings demonstrate that the presence of openings has a significant impact on the behavior of the beams, as there is a decrease in both the ultimate load and maximum deflection.

• Food Science of Animal Resources
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• v.44 no.5
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• pp.1080-1095
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• 2024

In contemporary society, the increasing number of pet-owning households has significantly heightened interest in companion animal health, expanding the probiotics market aimed at enhancing pet well-being. Consequently, research into the gut microbiota of companion animals has gained momentum, however, ethical and societal challenges associated with experiments on intelligent and pain-sensitive animals necessitate alternative research methodologies to reduce reliance on live animal testing. To address this need, the Fermenter for Intestinal Microbiota Model (FIMM) is being investigated as an in vitro tool designed to replicate gastrointestinal conditions of living animals, offering a means to study gut microbiota while minimizing animal experimentation. The FIMM system explored interactions between intestinal microbiota and probiotics within a simulated gut environment. Two strains of commercial probiotic bacteria, Enterococcus faecium IDCC 2102 and Bifidobacterium lactis IDCC 4301, along with a newly isolated strain from domestic dogs, Lactobacillus acidophilus SLAM AK001, were introduced into the FIMM system with gut microbiota from a beagle model. Findings highlight the system's capacity to mirror and modulate the gut environment, evidenced by an increase in beneficial bacteria like Lactobacillus and Faecalibacterium and a decrease in the pathogen Clostridium. The study also verified the system's ability to facilitate accurate interactions between probiotics and commensal bacteria, demonstrated by the production of short-chain fatty acids and bacterial metabolites, including amino acids and gamma-aminobutyric acid precursors. Thus, the results advocate for FIMM as an in vitro system that authentically simulates the intestinal environment, presenting a viable alternative for examining gut microbiota and metabolites in companion animals.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.12
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• pp.1126-1133
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• 2010

A two-stage biofilter was constructed and utilized to determine the removal efficiency when treating dynamic loading of a mixture of odorous compounds including benzene, toluene, p-xylene, ammonia and hydrogen sulfide. A yeast strain, Candida tropicalis, and a sulfur oxidizing bacterial (SOB) strain, Acidithiobacillus caldus sp., were immobilized in polyurethane media and packed in the two-stage biofilter. The experiment of dynamic loading variation was composed of (1) stepwise loading variation of all the odorous compounds (total EC test), (2) stepwise loading variation of each odorous compound, and (3) intermittent loading variation with 2-day-off and 3-day-on. The total EC test showed that the maximum elimination capacity was $61\;g/m^3/hr$ for total VOCs, and 5.2 and $9.1\;g/m^3/hr$ for ammonia and hydrogen, respectively. In addition, the inhibition between VOCs was observed when the loading of each individual VOC was varied. Especially the stepwise increase in toluene loading resulted in decreases of benzene and p-xylene removal efficiencies about 30% and 25%, respectively. However, the inhibition between organic and inorganic compounds was not observed. The intermittent loading variation with 2-day-off and 3-day-on showed that greater than 95% of the overall removal efficiency was restored in two days after the loading resumed. Consequently, the two-stage biofilter packed with immobilized microorganisms showed advantages over conventional biofilters for the simultaneous treatment of the mixture of organic and inorganic odorous compounds.