• 한국CDE학회논문집
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• 제14권6호
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• pp.374-381
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• 2009

To meet the soaring demand recently, South Korea big shipbuilders are examining two things. One is new investment in plant and equipment. The other is replacement of production resources. Considering plant & equipment investment and replacement of production resources, even if actual production ability would be enough, the real output could be affected by limitation of logistics with lack of analysis. As we set up big shipyard in virtual space, we could perform actual production by using confirm production plan in virtual space. We've analyzed the load of block stock, load of road and load of transporter for logistics effects are followed by production increase. This research is to determine the possible problems of those analyzed results and to present the resolution using the current layout. And then modified yard layout, we reanalyzed previous three logistics effects. This simulation model could help administrator to make rational decision for changing yard layout.

• 한국환경복원기술학회지
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• 제5권2호
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• pp.1-8
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• 2002

The aim of this study was to generate systemic data for the aquatic plant distribution according to water depth and crossing slope in the shoreline. The results of this study were as follows; 1. Anxiety to 0 near area Bidens frondosa, Scirpus tabernaemontani, Carex dispalata etc. total class 21 observe, and Phragmites communis, Iris pseudoacorus etc. class 6 of anxiety 0-70cm extent examined. Class 21 of anxiety observed all such as Bidens frondosa, Scirpus tabernaemontani, Carex dispalata in near area to 0, and Phragmites communis, Iris pseudoacorus etc. class 6 of anxiety 0-70cm extent examined. Anxiety 70-100cm extent Nymphoides indica, Ttapa japonica etc.. class 2 appeared to line Zizania latifolia, Typha angustata back 2 papers, 130cm and examined that Nelumbo nucifera was limit anxiety state 230cm. 2. Aquatic plants of Phragmites communis, Zizania latifolia, Typha angustata etc. range mainly to gentle gradient of slant 10 degree low and distribution pattern was ranging by Zizania latifolia, Typha angustata, Phragmites communis period of ten days from deepwater place. Nelumbo nucifera was forming become independent stock keeping away invasion of plant that ability to breed was different because was prosperous. Slant 10 bores was growing near sleep in been strange steep slope earth and distribution of emerged plant appeared punily and emerged plant and swampy land plant were ranged extensively in gentle gradient of 10 degree low. 3. On lake surrounding plant when wish to do distribution of natural conditions reference need to. That is, gentle gradient and distribution form of steep slope earth are different, and same pitch must consider enough this because appear as distribution, distribution according to that some plant species were growing was different.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2003년도 추계학술발표논문집
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• pp.230-248
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• 2003

본 연구에서는 제지공정에서의 wet-end 와 dry section 부분을 통합한 모델을 구하고 이를 바탕으로 하여 지종교체 공정의 모델예측제어 방법을 제안하였다. 폐회로 공정 인식기법을 이용하여 state-space 모델을 구한 후 지종교체 제어를 모사한 결과와 실제 제지공장의 지종교체 운전데이터를 비교 분석하였다. 입력 변수로서 이전까지는 간과되어 왔던 4가지 변수(thick stock, filler flow, speed, steam pressure), 그리고 출력변수로서 3가지 변수(basis weight, ash content, moisture content)를 고려하였으며, output trajectory는 1차 전달함수 형식으로 하여 적용하였다. 모델예측제어 모사결과를 지종교체 운전데이터와 비교하여 본 결과 지종교체 시간이 짧아지고 보다 안정적으로 정상상태에 이르는 것을 확인할 수 있었다. 아울러 모델예측제어로 인하여 지종교체 이후 입력 변수들이 큰 진동이 없이 보다 신속하게 정상상태에 도달함을 확인하였다.

• 식물조직배양학회지
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• 제28권1호
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• pp.33-36
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• 2001

고밀도 저수고형의 성공적인 왜화재배를 위한 사과 왜성대목인 M.9 T-337의 대량증식체계를 확립하기 위하여 실험한 결과를 요약하면 다음과 같다. 초기 생장점 배양은 채취한 경정조직을 ascorbic acid와 citric acid를 각각 100 mgL$^{-1}$와 150mgL$^{-1}$로 혼합한 용액에 30분간 전처리한 다음, IBA 0.1 mgL$^{-1}$, GA 0.5 mgL$^{-1}$와 sucrose 30 gL$^{-1}$가 첨가된 MS 액체배지에서 50 rpm으로 2주간 진탕배양한 후, 한천 8 gL$^{-1}$가 첨가된 동일조성의 MS 배지에서 배양하는 것이 신초형성에 가장 좋았다. 발근은 증식된 신초를 1주일간 Hormex 1,000배 액이 첨가된 1/2 MS 기본배지에 IBA 0.5 mgL$^{-1}$, sucrose 30 gL$^{-1}$ 등이 함유된 배지에서 50 rpm으로 진탕배양한 다음 Hormex를 제외한 동일 조성의 한천배지에 옮겨 배양하였을 때 배양된 모든 유묘로부터 정상적인 발근을 유도할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1511-1516
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• 2018

In this study, we investigated Indole-3-acetic acid (IAA) production by a rice phylloplane bacteria, Enterobacter sp. DMKU-RP206, using sweet whey as a feed stock instead of lactose. We succeeded in using sweet whey for Enterobacter sp. DMKU-RP206 to produce 3,963.0 mg IAA/l with the optimal medium containing 1.48% sweet whey, 1.42% yeast extract and 0.88% $\text\tiny{L}$-tryptophan. The medium pH was adjusted to 6 and the culture conditions were shaking at 200 rpm on an orbital shaker at $30^{\circ}C$ for 3 days. We also evaluated the effect of IAA in culture filtrates of Enterobacter sp. DMKU-RP206 on the promotion of jasmine rice growth in a pot experiment. Compared with the negative control (without IAA), the result showed that biosynthetic IAA produced by Enterobacter sp. DMKU-RP206 significantly increased the growth of jasmine rice (Oryza sativa L. cv. KDML105) in terms of length and dry weight of shoot. This work thus reveals the impact of IAA produced by Enterobacter sp. on the promotion of jasmine rice growth.

• 한국식품조리과학회지
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• 제18권1호
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• pp.13-19
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• 2002

신선, 열풍건조, 동결건조 천궁(Cnidium officinale)을 대상으로 향신료로의 이용가능성을 검토하기 위한 기초 실험으로 건조방법을 달리한 천궁 자체에 대한 관능평가 및 이들이 육류의 누린내 및 생선의 비린내에 미치는 영향을 관능검사 하였다. 관능검사 결과 천궁자체에 대한 관능검사에서는 신선, 동결건조, 열풍건조 시료 순으로 전체적인 선호도가 좋게 나타났으며, 천궁의 산뜻한 향과 노르스름한 색에 대한 선호도가 높았다. 신선, 열풍건조 및 동결건조 천궁 0.1% 첨가는 돼지고기의 누린내 및 고등어의 비린내를 제거하였으며 전체적인 선호도도 무첨가군보다 유의적으로 높았다. 쇠고기 및 닭고기 stock에서도 건조 천궁을 첨가함으로써 stock의 누린내를 제거하면서 색이나 맛에 나쁜 영향을 미치지 않았다. 따라서 천궁은 강한 향을 선호하는 소비자에게 알맞은 향신료로 사용될 수 있다고 판단된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.198-204
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• 2005

Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

• Animal cells and systems
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• 제16권5호
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• pp.400-407
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• 2012

Plant growth regulators are the chemicals that are found in plants and produced synthetically. In agricultural applications, plant hormones are used in minor quantities for fixing the problems. In our research, we studied the effects of 2,4 diclorophenoxyacetic acid (2,4D), which is an auxin used in agricultural applications. Auxins are the group that are used most popularly in plant growth regulators. In our study, different doses of 2,4 diclorophenoxyacetic acid are given to zebrafish, and ovarium tissues are observed histomorphologically. We generated one control and three experiment groups from the stock solution. The experiment was carried out in 20 liter capacity complete glass aquarium at $24{\pm}1^{\circ}C$ water temperature. After five days of application, fishes were dissected. Histomorphological changes of the ovarium were investigated under a light microscope. A decrease in the number of oocytes in zebrafish ovarium was observed when compared with the control group. Many deformed and underdeveloped oocytes were detected. An increase in the number of atretic oocytes was observed. It was deduced that acute doses of 2,4 diclorophenoxyacetic acid decelerates oogenesis in fishes.

• 식물병연구
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• 제19권1호
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• pp.25-30
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• 2013

국내에서 시판되고 있는 토마토 36품종을 이용하여 국내 시설재배지에서 가장 많이 분포하고 있는 두 종의 뿌리혹선충, Meloidogyne arenaria와 M. incognita에 대한 저항성을 검정하였다. 방울토마토 중에서는 '텐텐', '캐딜락', '큐티', '스위트', '뽀또', '리코핀-9' 등 6품종, 완숙토마토 중에서 '러브리240', '도테랑다이아', '큐피랑', '도테랑마스터', '슈퍼도태랑', '도태랑시즌', '마이로꾸', '호용' 등 8품종, 대목은 '스페셜', '파이팅', '마그네트' 등 3품종이 두 종류의 뿌리혹선충에 모두 강한 저항성이었다.

• The Plant Pathology Journal
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• 제35권2호
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• pp.164-171
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• 2019

An assay for detecting Apple scar skin viroid (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with realtime detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular beacon for detecting the viroid were designed based on highly conserved regions of several ASSVd sequences including Korean isolate. The assay had a detection range of $1{\times}10^4$ to $1{\times}10^{12}$ ASSVd RNA $copies/{\mu}l$ with reproducibility and precision. Following the construction of standard curves based on time to positive (TTP) value for the serial dilutions ranging from $1{\times}10^7$ to $1{\times}10^{12}$ copies of the recombinant plasmid, a standard regression line was constructed by plotting the TTP values versus the logarithm of the starting ASSVd RNA copy number of 10-fold dilutions each. Compared to the established RT-PCR methods, our method was more sensitive for detecting ASSVd. The real-time quantitative NASBA method will be fast, sensitive, and reliable for routine diagnosis and selection of viroid-free stock materials. Furthermore, real-time quantitative NASBA may be especially useful for detecting low levels in apple trees with early viroid-infection stage and for monitoring the influence on tree growth.