• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.75-80
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• 2000

An improved method of refolding recombinant human proinsulin from E. coli was presented. It was based on a two-stage stirred tank reactor in which denatured proinsulin-s-sulfonate was mixed instantaneously with a reaction buffer in the first stage reactor, and then fed to the second stage reactor. The mixture was stirred further for a total of 30h in the second stage reactor. In this system, unfavorable effects present due to the increase in reaction volume and protein concentration for protein refolding, which becomes significant in a large-scale operation, were avoided. Refolding yields of over 80% was obtained for achieving reaction volume of upto 50 l at protein concentration of 1 mg/ml. The optimum urea concentration was 1M. Refolding yield at the 1-1 reaction volume and protein concentration of 0.5mg/ml was increased about 2.5-fold, compared to that in a batch reactor. By increasing protein concentration in a two-stage refolding reaction, the cost for insulin production could be reduced, therefore, making this process economical.

• 비파괴검사학회지
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• 제26권1호
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• pp.1-6
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• 2006

폐수처리시설의 응집조를 대상으로 0.392MeV의 감마선을 방출하는 In-113m을 추적자로 이용하여 체류시간분포(RTD; residence time distribution)를 측정하였다. 계측 결과는 CFSTR (constant flow stirred tank reactor) 모델을 바탕으로 구축한 K-RTD 프로그램을 이용하여 수학적으로 분석하였으며, 이를 바탕으로 혼합 특성을 규명하고자 하였다 모델에 의한 시뮬레이션 결과를 계측결과와 맞추는 과정에서 유체 거동의 특성을 표현하는 인자들을 계산하였으며, 이들 인자에 의한 시뮬레이션 결과는 실험결과에 매우 성공적으로 부합하였다. 또한 향후 본 실험에서 관찰된 bypass flow의 성분을 이론적 모델에 포함시키고 이를 분석하며 혼합조의 효율에 영향을 미치는 요소들에 대한 추가 연구의 필요성을 확인하였다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2005년도 추계 학술대회논문집
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• pp.31-34
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• 2005

This article has investigated the spatial distribution of the solid particles in polymerization reactors using CFD analysis (FLUENT v. 6.2.1). The suspension of the solids in stirred reactors is affected by a number of parameters including particle diameter, vessel shape, impeller size, impeller speed, and rotating direction of stirrer. The degree of solids suspension in the vessel was quantified with a statistical average value, ${\sigma}^2$. The best stirring conditions were determined based on ${\sigma}^2$, which was found to depend on the vessel bottom shape.

• 대한기계학회논문집B
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• 제27권4호
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• pp.504-510
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• 2003

Simultaneous measurements of turbulent velocity and concentration field in a stirred mixer tank are carried out by using PIV/LIF technique. Instantaneous velocity fields are measured with a 1K$\times$1K CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.236-239
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• 2008

In the present study, a transient glass particle distribution in a stirred solid/liquid mixer was investigated using computational fluid dynamics(CFD). The flow patterns and solid concentaration distriburion in a solid/liquid mixer formed by pitched paddle and baffles were predicted. The numerical results were compared to experimental data from the available literature. Eulerian multi-phase model was used to investigate the influence of the density of solid particle on the same impeller speed. A good agreement was obtained between the experimental data and simulation results.

• KSBB Journal
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• 제27권1호
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• pp.28-32
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• 2012

In this study, the treatment ability of the wastewater containing perchlorate by non-salt tolerant perchlorate reducing bacterial consortium (N-PRBC) was evaluated in a continuous stirred tank bioreactor (CSTR). To obtain the optimal operating condition the bioreactor was operated with the different wastewater empty bed retention time (EBRT). The treatment performance in the bioreactor could be maintained at 100 $mg-ClO_4{^-}L^{-1}$ up to a EBRT of 3 h, and the removal capacity in the CSTR was about 3.3 times higher than that in a batch operation. With a decrease from 9 h to 2 h in a EBRT, the volumetric perchlorate reduction rate was increased from 11.1 $mg-ClO_4{^-}L^{-1}h^{-1}$ to 50.0 $mg-ClO_4{^-}L^{-1}h^{-1}$, and the specific perchlorate reduction rates were increased from 3.01 $mg-ClO_4{^-}g-DCW^{-1}h^{-1}$. In conclusion, the treatment capacities in a CSTR were much better than those obtained in a batch operation.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2002년도 추계학술대회 논문집
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• pp.101-104
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• 2002

Simultaneous measurement of turbulent velocity and concentration field in a stirred mixer tank is carried out by using PIV/LIF technique. Instantaneous velocity fields are measured by a $1K\times1K$ CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

• 항공우주시스템공학회지
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• 제13권1호
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• pp.1-10
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• 2019

교반기에서의 유동 특성은 광범위한 산업 분야에서 매우 유용하다. 일반적으로 교반되는 용기에서의 유동 패턴, 전력 소비 및 혼합 시간은 임펠러의 설계뿐만 아니라 용기 형상 및 내부 구조에 달려 있다. 본 연구에서는 베플 형상과 임펠러의 상호 작용에 의해 생성되는 불안정하고 비정상상태의 복잡한 유동 특성 분석을 ANSYS FLUENT LES 난류 모델을 사용하여 수행하였다. Axial Flow 와 Radial Flow 두 가지 타입의 회전 임펠러와 3가지 베플의 형상 사이의 상호 작용과 영향을 전산유체역학(CFD)으로 예측 비교함으로써 교반 시 임펠러와 베플 주변에서의 유동 특성과 혼합 유동장에서 상대적으로 효율적인 경향을 보이는 설계 모델을 검증할 수 있었다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.520.3-521
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• 1986

L-Lysine 생성균인 Corynebacterium glutamicum의 동종간 융합주 RS 99를 담체인 Alginate와 여기에 TiCl$_4$로부터 제조된 Titanium hydroxide를 혼합하여 각각 고정하고 이들의 Gel strength, 활성도 및 회분식 발효조건을 비교하였다. 그 결과 Alginate-Titanium hydroxide를 담체로 선정하여 고정화 C, glutamicum 융합주의 재사용성 및 안정성을 검토하였으며, Continuous-Flow Stirred-Tank Reactor를 구성하여 L-Lysine 의 연속발효를 시도하였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 한국자동제어학술회의논문집(국내학술편); 포항공과대학교, 포항; 24-26 Oct. 1996
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• pp.788-791
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• 1996

A mathematical model is developed for thermal solution copolymerization of styrene and acrylonitrile in a continuous stirred tank reactor(CSTR). Computational studies are carried out with the continuous copolymerization system model developed in this work to give the monomer conversion, copolymer composition and the average molecular weights of the copolymer. By performing the dynamic analysis of the reaction system, the polymer properties against the changes in the operating conditions are determined quantitatively. The cascade PID and fuzzy controller show satisfactory performances for both set point tracking and disturbance rejection. Especially, the fuzzy controller is superior to the PID controller.