• 한국균학회지
• /
• 제8권2호
• /
• pp.101-106
• /
• 1980

To investigate constituents of Lampteromyces japonicus (Kawam.) Singer which grows wildly in Korea, the carpophores of the fungus were collected in Gyeong Gi. Province and analyzed for sterols and amino acids by gas chromatography and an amino acid autoanalyzer. The results showed that it contained stigmasterol and ergosterol and that it contained eleven free amino acids. These free amino acids were also determined quantitatively. Furthermore, sixteen amino acids were identified in the total amino acid fraction of the fruiting body.

• Natural Product Sciences
• /
• 제14권1호
• /
• pp.56-61
• /
• 2008

Bioactivity guided phytochemical study of the petroleum ether and butanol extracts of Clematis hirsuta resulted in the isolation of 12 compounds. Rat paw edema as a model of acute inflammation was used to evaluate the anti-inflammatory activity of the extracts and the chromatographic fractions. Five known sterols and triterpenes namely: ${\beta}-amyrin$ (1), lupeol (2), ${\beta}-sitosterol$ (3), oleanolic acid (4) and stigmasterol glycoside (5) were isolated from the petroleum ether extract. The n-butanol extract afforded two compounds reported for the first time from natural source: (S)-(+)-dihydro-5-(hydroxymethyl)-2(3H)-furanone (7) and (s)-(-)-5-hydroxymethyl-2(5H)-furanone (8). In addition, anemonin (6), dihydro-4-hydroxy-5-(hydroxymethyl)-2(3H)-furanone (2-deoxy-D-ribono-1,4-lactone) (9), biophenol (cimidahurin) (10), glucose (11) and sucrose (12) were also identified. The structures were determined from spectroscopic data including 1D- and 2D-NMR experiments.

• 약학회지
• /
• 제22권2호
• /
• pp.87-90
• /
• 1978

"Bija"(Torreya nut), a tree belonging to the Torreyaceae family, is cultivated in Jae Joo Do and Jun Ra Do, Korea. The seed of Torreya nucifera, which has been widely used as folkmedicine in the treatment of tapeworm infestation, was examined on the sterol composition determined by gas liquid chromatography and thin layer chromatography on the preparative plates. Sterols were obtained from the nonsaponifiable matters of ether extract of the seed. It was noted that .betha.-sitosterol was the major sterol in the nut. The results showed that contents of sterols were campesterol 3.15-3.75%, stigmasterol 5.38-5.67%, $\beta$-sitosterol 85.61-86.28% and ${\delta}^{7}$-sterol 4.91-5.19% by gas liquid chromatography.

• Journal of Nutrition and Health
• /
• 제9권3호
• /
• pp.26-30
• /
• 1976

The contents of sterols in bean sprouts have been determined by mass spectrical analysis. From non-saponifiable fraction of n-Hexane extracts of the under ground parts sterol-like substance $(m.P.\;142-145^{\circ}C)$ was isolated. It was shown to be a sterol mixture on the basis of three peaks in gas liquid chromatogram. By mass spectrometrical analysis of the mixture, it was composed of 4.52mg% of Campesterol $(C_{28},\;{\Delta}^{5})$, 10.35mg% of ${\beta}-sitosterol\;(C_{29},\;{\Delta}^{5})$ and 7.75mg% of stigmasterol $(C_{29},\;{\Delta}^{5,\;22})$.

• 약학회지
• /
• 제20권1호
• /
• pp.37-40
• /
• 1976

${\beta}$-Sitosterol, stigmasterol, campesterol, cholestanol and a triterpenoid, ursolic acid were identified from the root of the Rubus crataegifolius B$_{UNUGE}$(Rosaceae).

• 약학회지
• /
• 제13권4호
• /
• pp.144-146
• /
• 1969

Sterols from several kinds of plants were investigated by means of gas chromatography, using 4mm * 1.8 mm glass column, packed with 1.5% SE 30 on Chromosorb W.

• 한국식품과학회지
• /
• 제12권1호
• /
• pp.6-12
• /
• 1980

한국산(韓國産) 목이(木耳) 석의중(石衣中) 지방산(脂肪酸) 조성(組成)과 스테롤 성분(成分)의 구명(究明)을 G.L.C.와 I.R. 분석(分析)에 의하여 검토(檢討)한 결과(結果) 다음과 같은 결과(結果)를 얻었다. G.L.C에 의(依)한 지방산(脂肪酸의 조성(組成)은 목이(木耳)의 경우 linoleic acid 33.73, palmitic acid 15.52, stearic acid 5.03, oleic acid 16.03, linolenic acid 17.80 및 미지산(未知酸) 11.89%를 나타낸 반면(反面), 석의(石衣)는 linoleix acid 46.35, palmitic acid 31.71, oleic acid 16.82, 미지산(未知酸) 5.12% 및 소량(小量)의 stearic acid와 linolenic acid를 보여주었다. 스테롤 성분(成分)을 T.L.C.에 의(依)하여 분리(分離)한 후(後) I.R. 분석(分析)으로 구명(究明)한 결과(結果) 목이(木耳)와 석의(石衣) 모두 sitosterol과 ergosterol을 함유(含有) 하였다.

• 한국환경과학회지
• /
• 제14권5호
• /
• pp.481-490
• /
• 2005

Surface sediments and seawater were sampled at Masan and Haengam Bays of Korea, to evaluate contamination by sewage-derived organic matter using fecal sterols, Six stream-water samples into Masan and Haengam Bays were also sampled. Total concentrations of eight sterols (coprostanol, epichloestanol, epicoprostanol, cholesterol, cholestanol, brassicasterol, stigmasterol and $\beta-sitosterol$) were in the range of $1,274\~4,768{\mu}g/g$ dry weight in suspended particulate from the stream-water, $292\~2,244{\mu}g/g$ dry weight in suspended particulate from the seawater and $4.5\~27.2{\mu}g/g$ dry weight in the sediments. Although sterol compositions in sediments, seawater and stream-water were different, cholesterol was the predominant sterol in all samples. The proportion and concentration of coprostanol, a sewage tracer, in stream-water was much higher than those in sediment and seawater. The sterol levels including coprostanol in the sediments and seawater were higher at inner basin than at the outer bay. Some molecular indices and multivariate statistical analysis were used to assess the origin of these sterols and sewage contamination in the study area. The sterol composition patterns in stream-water were mainly associated with contamination by sewage-derived organic matters and those of seawater were associated with the activities of marine-originated organisms. Sterol levels in the sediments were both from the sewage input through stream-water and the marine- originated organisms. This survey suggests that the main source/route of sewage-derived organic matters in Masan Bay is the input of stream-water into the bay.

• 생약학회지
• /
• 제39권3호
• /
• pp.186-193
• /
• 2008

Astragali Radix, known as Huangqi, is the most important tonic in the traditional oriental medicine. It reinforces 'qi' (vital energy), strengthens the superficial resistance and promotes the discharge of pus and the growth of new tissue. It has long been used as an anti-perspirant, anti-diuretic or a tonic. Eleven compounds were isolated from the hexane and EtOAc fractions from the roots of Astragalus membranaceus (Leguminosae) and their structures were identified as four triterpenoids [lupenone (1), friedelin (2), lupeol (3), soyasapogenol E (9)] and seven sterols [${\beta}-sitosterol$ (4), stigmastane-3,6-dione (5), $7{\alpha}-hydroxysitosterol$ (6), $5{\alpha},6{\beta}-dihydroxysitosterol$ (7), $7-oxo-{\beta}-sitosterol$ (8), ${\beta}-sitosterol$ glucoside 6'-O-palmitate (10), ${\beta}-sitosterol$ glucoside (11)]. The chemical structures of these compounds were identified on the basis of spectroscopic methods and comparison with literature values. Among these compounds, lupenone (1), friedelin (2), lupeol (3), stigmastane-3,6-dione (5), $7{\alpha}-hydroxysitosterol$ (6), $5{\alpha},6{\beta}-dihydroxysitosterol$ (7), $7-oxo-{\beta}-sitosterol$ (8), soyasapogenol E (9), and ${\beta}-sitosterol$ glucoside 6'-O-palmitate (10) were isolated from this plant for the first time.

• 한국식품과학회지
• /
• 제24권1호
• /
• pp.82-85
• /
• 1992

가스크로마토그라프법으로 마요네즈 중의 스테롤을 정량하는데 있어서 충전컬럼과 모세관 컬럼을 사용하여 비교 연구하였다. 두 컬럼의 경우 4종의 스테롤(cholesterol, campesterol, stigmasterol, ${\beta}-sitosterol$)이 모두 뚜렷히 분리되었으나 모세관 컬럼이 신속성, 재현성, 분리능 면에서 우수하였다. 한편, 제품에 적용하여 분석한 결과, 측정된 cholesterol 함량으로부터 추정한 시판 마요네즈 난황사용량은 충전컬럼의 경우 $2.69{\sim}7.11%$, 모세관 컬럼의 경우 $2.42{\sim}6.08%$ 범위이었으며, 스테롤 조성으로부터 마요네즈 제조에 사용된 식용유는 대두유 및 면실유임을 알 수 있었다.