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Bioactivity Guided Phytochemical Study of Clematis hirsuta Growing in Saudi Arabia  

Abdel-Kader, Maged S. (Department of Pharmacognosy, College of Pharmacy, King Saud University)
Al-Taweel, Areej M. (Department of Pharmacognosy, College of Pharmacy, King Saud University)
El-Deeb, Kadriya S. (Department of Pharmacognosy, College of Pharmacy, King Saud University)
Publication Information
Natural Product Sciences / v.14, no.1, 2008 , pp. 56-61 More about this Journal
Abstract
Bioactivity guided phytochemical study of the petroleum ether and butanol extracts of Clematis hirsuta resulted in the isolation of 12 compounds. Rat paw edema as a model of acute inflammation was used to evaluate the anti-inflammatory activity of the extracts and the chromatographic fractions. Five known sterols and triterpenes namely: ${\beta}-amyrin$ (1), lupeol (2), ${\beta}-sitosterol$ (3), oleanolic acid (4) and stigmasterol glycoside (5) were isolated from the petroleum ether extract. The n-butanol extract afforded two compounds reported for the first time from natural source: (S)-(+)-dihydro-5-(hydroxymethyl)-2(3H)-furanone (7) and (s)-(-)-5-hydroxymethyl-2(5H)-furanone (8). In addition, anemonin (6), dihydro-4-hydroxy-5-(hydroxymethyl)-2(3H)-furanone (2-deoxy-D-ribono-1,4-lactone) (9), biophenol (cimidahurin) (10), glucose (11) and sucrose (12) were also identified. The structures were determined from spectroscopic data including 1D- and 2D-NMR experiments.
Keywords
Clematis hirsuta; triterpenes; sterols; anemonin; anti-inflammatory;
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