• 한국식품영양과학회지
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• 제14권4호
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• pp.365-369
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• 1985

참기름중(中) 부감화물은 RWE가 3.1%이고 RTE는 2.6%이며 전(全) sterol은 RTP가 0.68%이고 RTE가 0.48%이며 전(全) sterol중(中) free sterol이 37.9 $(RTP){\sim}52.7%(RTE)$로서 sterylglycoside 및 sterylester보다 그 함량(含量)이 많다. 참기름중(中) total sterol의 구성 sterol조성(組成)은 $39.3{\sim}42.9%$의 sitosterol, $13.0{\sim}17.2%$의 ${\Delta}^5-avenasterol$, $9.1{\sim}11.0%$의 campesterol 및 $7.4{\sim}11.5%$의 stigmasterol이며 $23.5{\sim}24.6%$의 미지(未知)sterol (RRT:1.35)도 함유(含有)되어 있었고 sterylglycoside는 total sterol의 sterol조성(組成)과 거의 같은 경향(傾向)이었으나 free sterol과 sterylester는 ${\Delta}^5-avenasterol$이 $8.1{\sim}11.4%$로서 campesterol 및 stigmasterol보다 함유비(含有比)가 낮았다. 한편 채유방법별(採油方法別) sterol조성(組成)은 차이(差異)가 거의 없었다.

• 한국식품과학회지
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• 제9권1호
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• pp.41-46
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• 1977

1. 무환자(無患子)나무종자중(種字中)의 유지함량(油脂含量)은 45.7%로서 콩, 참깨, 땅콩에 비해서 많은 편에 속한다. 2. 지방산(脂肪酸)은 Oleic acid(61.6%)와 Gadoleic acid(20.4%)로서 주요성분(主要成分)을 이루고 있고 monoene산(酸)이 전체지방산(全體脂肪酸)의 82%를 차지하고 있는것이 특징적(特徵的)이다. 3. Amino산조성(酸組成)은 Glutamic acid의 함량(含量)이 6.673%로서 가장높고 대체적으로 필수(必須) amino산(酸)인 Isoleucine(1.255%), Leucine(2.145%), Lysine(2.191%), Phenylalanine(1.536%), Threonine(1.171%), Valine(1.772%)등이 함량(含量)이 높았다. 4. Sterol조성(組成)은 4-desmethyl sterol, 4-monomethyl sterol fraction에서 다 같이 일반고등식물중(一般高等植物中) 성분(成分)과 대차(大差)가 없었으며 단지 4-monomethyl sterol fraction에 있는 RRT 1.27(31.5%)의 미동정물질(未同定物質)이 있다는 것이 특이(特異)하였다.

• BMB Reports
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• 제31권6호
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• pp.565-571
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• 1998

The expression of the endogenous human apolipoprotein(apo)E gene was significantly induced when HepG2 cells were treated with exogenous 25-hydroxy-cholesterol. This sterol-mediated apoE gene upregulation appears to require the participation of a positive element for the apoE gene transcription (PET) ( -169/ -140), a core sequence of upstream regulatory element (URE)1 enhancer of the human apoE gene. This PET was renamed as sterol regulatory element (SRE)4 based on its new role as a sensor for the level of intracellular sterol. Furthermore, a gel mobility shift analysis showed that binding activity of the SRE4 binding protein (BP) obtained from HepG2 cells was induced by sterol treatment, while that from either MCF7 or BT20 cells remained unchanged. Binding activity of SRE4BP was also induced in mouse macrophage cells, J774A.1, by sterol treatment, but it was drastically reduced when cells were subjected to treatment of AY-9944, a potent inhibitor for sterol synthesis. However, binding activity of Spl, which is a co-binding protein to the SRE4 region, remained the same in either condition, suggesting that SRE4BP (formally known as PETBP) may be mainly responsible for the sterol-mediated regulation of the apoE gene expression. Deletion analysis of the core binding site of SRE4BP by gel mobility shift assays showed that the minimal sequence of the SRE4BP binding appears to reside between -157 and -140, confirming the identity of SRE4 with the previously determined core sequence of URE1.

• 한국약용작물학회지
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• 제5권2호
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• pp.113-118
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• 1997

미강에 함유된 약리성분인 ${\gamma}-oryzanol$ 함량을 검정하여 용도 다양화 연구의 기초 자료를 제공코자 시험을 수행한 바 다음과 같은 결과를 얻었다. Soxhlet 추출장치에서 얻은 미강유에서 유리 sterol과 ferulic acid ester가 분리되었다. 유리 sterol은 GC-MS에 의하여 campesterol, ${\beta}-sitosterol$ 및 stigmasterol로 구성되어 있음을 알 수 있었다. ferulic acid ester는 알칼리 가수분해에 의하여 얻어지는 검화분해물을 분리용 TLC로 분리하여 triterpene과 sterol을 얻었다. sterol은 GC-MS 측정으로 유리 sterol과 같은 조성임을 알았으며 triterpene은 cycloartanol, cycloartenol, 24-methylenecycloartanol 및 미지의 triterpene로 구성되었다. 미강유나 pitch 중 ferulic acid ester의 흡광도를 기준으로 ${\gamma}-oryzanol$의 함량을 평가한다면 큰 오차는 없으리라 생각된다.

• Natural Product Sciences
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• 제9권4호
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• pp.299-303
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• 2003

For the screening of inhibitors of sterol biosynthesis from natural products, a simple and rapid assay method was developed using recombinant yeast carrying human lanosterol synthase, main target of this assay method. Sterol biosynthesis inhibition activity was monitored only by the inhibition of growth of the recombinant yeast. By changing the substrate, this assay method can figure out which step is inhibited in the sterol biosynthesis by the test material. With this assay method total 102 plant samples were screened for their inhibitory activity of sterol biosynthesis. Among plant water extracts screened, 11 plant samples showed inhibitory activity on sterol biosynthesis in ergosterol (-) medium. For selection of the specific inhibitory materials, 11 plant samples were reassayed in ergosterol (+) medium. After all 5 plant samples, Abutilon avicennae Gaertn. (stem), Alnus japonica Steud. (stem), Amaranthus mangostanus L. (aerial part), Philadelphus schrenckii Pupr. (leaf) and Pimpinelia brachycarpa Nakai (aerial part), showed specific inhibitory activity.

• Applied Biological Chemistry
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• 제27권2호
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• pp.119-128
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• 1984

미강유(米糠油)의 부(不)검화물(化物)을 분석(分析)하여 sterol조성(組成)을 살펴본 결과(結果)는 다음과 같았다. 4-Desmethylsterol로서는 10개가 동정(同定)되었으며 이 중(中) sitosterol이 주성분(主成分)을 이루고 있었다. 2개의 소량성분(少量成分)인 ${\Delta}^7$-stigmastenol(3%)과 ${\Delta}^7$-avenasterol(1%)를 제외(除外)하면 모두가 ${\Delta}^5$-sterol였으며 소위(所謂) ${\Delta}^5$형(型) sterol분포(分布)를 보이고 있었다. 4-monomethylsterol fraction에서는 9개가 확인(確認)되었으나 cycloeucalenol, citrostadienol, gramisterol가 주성분(主成分)이었다. 환계(環系)의 구조가 다양(多樣)하여 sterol의 생합성경로(生合成經路)의 중간단계(中間段階)임을 보여주고 있다. 4,4-Dimethylsterol(triterpene alcohol)로서는 4개가 동정(同定)되었으나 이들은 모두 9,19-cyclopropanering을 가지고 있다. 이들중(中) sterol생합성(生合成)의 초기단계(初期段階)의 물질(物質)인 cycloartenol와 24-methylenecyclo-artenol가 이 fraction의 96%를 점(占)하고 있는 것으로 봐서 미(米)강의 4,4-dimethylsterol는 짧은 경로(經路)를 거쳐 쉽게 4-monomethylsterol로 전환(轉換)되는 것 같다. Side chain에서 E-, Z-관계(關係)인 이성체(異性體)가 4-desmethylsterol로서는 fucosterol와 ${\Delta}^5$-avenasterol가 그리고 4-monomethylsterol로서는 28-isocitrostadienol와 citrostadienol로서 각각 1쌍식이 검출(檢出)된 것으로 미루어 볼때 sterol의 생합성과정(生合成過程)에서 side chain에서 보다 환계(環系)에서 먼저 변환(變換)이 일어나는 것으로 보아진다.

• 한국환경과학회지
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• 제14권5호
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• pp.481-490
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• 2005

Surface sediments and seawater were sampled at Masan and Haengam Bays of Korea, to evaluate contamination by sewage-derived organic matter using fecal sterols, Six stream-water samples into Masan and Haengam Bays were also sampled. Total concentrations of eight sterols (coprostanol, epichloestanol, epicoprostanol, cholesterol, cholestanol, brassicasterol, stigmasterol and $\beta-sitosterol$) were in the range of $1,274\~4,768{\mu}g/g$ dry weight in suspended particulate from the stream-water, $292\~2,244{\mu}g/g$ dry weight in suspended particulate from the seawater and $4.5\~27.2{\mu}g/g$ dry weight in the sediments. Although sterol compositions in sediments, seawater and stream-water were different, cholesterol was the predominant sterol in all samples. The proportion and concentration of coprostanol, a sewage tracer, in stream-water was much higher than those in sediment and seawater. The sterol levels including coprostanol in the sediments and seawater were higher at inner basin than at the outer bay. Some molecular indices and multivariate statistical analysis were used to assess the origin of these sterols and sewage contamination in the study area. The sterol composition patterns in stream-water were mainly associated with contamination by sewage-derived organic matters and those of seawater were associated with the activities of marine-originated organisms. Sterol levels in the sediments were both from the sewage input through stream-water and the marine- originated organisms. This survey suggests that the main source/route of sewage-derived organic matters in Masan Bay is the input of stream-water into the bay.

• 한국균학회지
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• 제11권2호
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• pp.97-98
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• 1983

이 실험의 결과에 의하여 한국산 말뚝버섯 Phallus impudicus (Fr.) $Qu\acute{e}l$.의 sterol성분으로서 ergosterol을 확인할 수 있었다.

• 한국자기공명학회논문지
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• 제11권2호
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• pp.122-128
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• 2007

Three sterol sulfates were isolated from AMPK activity-guided fraction of a marine sponge Acanthodoryx fibrosa. Their structures were determined by an extensive NMR analysis, MS data, and two compounds were confirmed as unusual phosphorylated sterol sulfates by comparing with NMR data of the known compounds. Compound 3 was given to be a new dephosphated sterol sulfate derivative. Compound 1 moderately showed AMPK activation effect on L6 myoblast cell through Western blot analysis.

• 한국식품영양과학회지
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• 제28권5호
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• pp.980-983
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• 1999

The contents of sterols and stanols in vegetable oils and mist oil were analyzed by gas chromatography using a capillary column. The total sterol contents showed high values of 0.67~0.89g/100g in corn oil, rice bran oil, red pepper seed oil and sesame oil. Mist oil, a byproduct of soybean oil manufacture, was a suitable raw material for the production of stanol since it showed high sterol content (10.2g/100g). In the hydrogenation of sterol contained in mist oil using Pd catalyst, the effects saponification of oil were examined. The conversion of sterol to stanol was improved by a factor of 4~5 through saponification of oil, compared to the reaction without saponification.