Abstract
In order to examine the effect of oil extraction methods on the characteristics of sesame oil, the unsaponifiable matters, fractionation sterol pattern and sterol compositions of the each fraction of the oil were compared in the oil extracted by the three different extraction methods, that is, pressure extraction of roasted seed (RTP), acetone extraction of roasted seed(RTE) and acetone extraction of raw seed(RWE). The amount of unsaponifiable in RWE oil was silghly higher as 31.8mg per 1mg drying oil than that in RTP oil of 26.1mg. Sesame oils from three different extraction methods were found to contain $0.26{\sim}0.32%$ free, $0.23{\sim}0.42%$ bound, and $0.49{\sim}0.64%$ total sterol. The content of free sterol in RWE oil was higher as 0.32% than that in RTE and RTP oil of 0.26%, and that of sterylglycoside in RTE oil was lower as 0.12% than that in RTP and RWE oil of 0.23%, but that of sterylester was a little difference. The unsaponifiable matter from fractionation sterol in sesame oil by three different extraction methods was fractionated into less polor compounds, 4,4-dimethyl-, 4-monomethyl-, 4-desmethylsterol fraction by thinlayer chromatography, and sterol composition of 4-desmethylsterol fraction was analyzed by gas liquid chromatography. The major sterols were campe-, stigma-, sito-, and ${\Delta}^5-avenasterol$, but, specially, unknown sterol(RRT:1.35) was found as $23.5{\sim}26.4%$ in total sterols, The content of sitosterol, ${\Delta}^5-avenasterol$, campesterol and stigmasterol were $59.9{\sim}60.3%,\;8.1{\sim}11%,\;16.1{\sim}18.4%,\;11.6{\sim}12.8%$ of the total sterol in free sterol fraction, $37.3{\sim}46.9,\;11.6{\sim}14.2,\;6.6{\sim}9.0$, and $6.1{\sim}8.0%$ of the total sterol in sterylglycoside fraction, $55.9{\sim}59.9,\;9.2{\sim}11.4,\;17.1{\sim}18.9$, and $11.8{\sim}13.7%$ of the total sterol in sterylester fraction, and $39.3{\sim}42.9,\;13.0{\sim}17.2,\;9.1{\sim}11.0$ and $7.4{\sim}11.5%$ of the total sterol in total sterol fraction. But the effect of oil extraction methods on sterol composition in sesame oil were hardly found.
참기름중(中) 부감화물은 RWE가 3.1%이고 RTE는 2.6%이며 전(全) sterol은 RTP가 0.68%이고 RTE가 0.48%이며 전(全) sterol중(中) free sterol이 37.9 $(RTP){\sim}52.7%(RTE)$로서 sterylglycoside 및 sterylester보다 그 함량(含量)이 많다. 참기름중(中) total sterol의 구성 sterol조성(組成)은 $39.3{\sim}42.9%$의 sitosterol, $13.0{\sim}17.2%$의 ${\Delta}^5-avenasterol$, $9.1{\sim}11.0%$의 campesterol 및 $7.4{\sim}11.5%$의 stigmasterol이며 $23.5{\sim}24.6%$의 미지(未知)sterol (RRT:1.35)도 함유(含有)되어 있었고 sterylglycoside는 total sterol의 sterol조성(組成)과 거의 같은 경향(傾向)이었으나 free sterol과 sterylester는 ${\Delta}^5-avenasterol$이 $8.1{\sim}11.4%$로서 campesterol 및 stigmasterol보다 함유비(含有比)가 낮았다. 한편 채유방법별(採油方法別) sterol조성(組成)은 차이(差異)가 거의 없었다.