• Journal of Microbiology and Biotechnology
• /
• 제8권1호
• /
• pp.28-33
• /
• 1998

A gene coding for ${\beta}$-xylosidase from thermophilic xylanolytic Bacillus sp. KK-1 was cloned into Escherichia coli using plasmid pBR322. Recombinant plasmid DNAs were isloated from E. coli clones which were capable of hydrolyzing 4-methylumbelliferyl-${\beta}$-D xylopyranoside. Restriction analysis showed the DNAs to share a common insert DNA. Xylo-oligosaccharides, including xylotriose, xylotetraose, xylopentaose, and xylobiose were hydrolyzed to form xylose as an end product by cell-free extracts of the E. coli clones, confirming that the cloned gene from strain KK-1 is ${\beta}$-xylosidase gene. The ${\beta}$-xylosidase gene of strain KK-1 designated as xylB was completely sequenced. The xylB gene consisted of an open reading frame of 1,602 nucleotides encoding a polypeptide of 533 amino acid residues, and a TGA stop codon. The 3' flanking region contained one stem-loop structure which may be involved in transcriptional termination. The deduced amino acid sequence of the KK-1 ${\beta}$-xylosidase was highly homologous to the ${\beta}$-xylosidases of Bacillus subtilis and Bacillus pumilus, but it showed no similarity to a thermostable ${\beta}$-xylosidase from Bacillus stearothermophilus.

• 대한조선학회논문집
• /
• 제36권1호
• /
• pp.61-69
• /
• 1999

수치 계산을 이용하여 선형의 유체동력학적인 특성을 신속히 파악하기 위해서는 선체표면 격자계를 생성하는 과정이 쉽고 자동화되어 있어야 한다. 이를 위하여 기본 스테이션 오프셋과 선수미 윤곽선만을 이용하여 선형을 정의하고, 이를 이용하여 퍼텐셜 유동이나 점성유동의 계산에 필요한 선체 표면 격자계를 손쉽게 생성할 수 있는 방법을 개발하였다. 선수와 선미 벌브를 가진 일반 상선의 선형을 표현하기 위해 수선의 양 끝단을 타일, 포물선, 쌍곡선, 3차 곡선 등의 조합으로 표시할 수 있게 하였다. 그리고 선수와 선미의 윤곽선을 격자계의 경계선으로 하였기 때문에 비선형 자유수면 조건을 이용한 조파저항 계산에서의 격자 변형이나 점성유동 해석을 위한 난류 경계층에서의 벽좌표의 정의가 종단면을 기준으로 한 종래의 격자에 비해 매우 편리하다고 할 수 있다.

• 대한조선학회논문집
• /
• 제36권1호
• /
• pp.70-81
• /
• 1999

선체주위의 점성유동을 계산하기 위해서는 수치계산을 위한 3차원 공간 격자계가 필요하다. 본 논문에서는 타원형 미분 방정식인 Poisson 방정식의 해를 이용하여 3차원 공간 격자계를 구성하는 방법을 개발하였다. 2차원에서 사용되던 Sorenson방법을 3차원으로 확장하여 격자계의 분포 및 교차 각도를 지정하는 함수를 정의하게 하였다. 미분방정식의 해는 weighting function scheme과 modified strongly implicit procedure를 사용하여 구하였고, 3차원 내부 격자계와 경계면과의 매끄러운 연결을 위해 trans-finite interpolation을 이용하였다. 적용예로서 컨테이너 운반선과 대형 유조선 주위의 난류유동 계산을 위한 공간 격자계를 보였다.

• 한국해양공학회지
• /
• 제22권4호
• /
• pp.84-89
• /
• 2008

It is hard to find experimental data for a model test of small high-speed planning boats. It is difficult to verify the performance seen in a model test for a high-speed boat because the ship-model scale-ratio is very small and the flow velocity of the circulating water channel and the X-carriage speed of the towing tank are restricted. Therefore most hull-form designs for high-speed small boats depend on the sea-trial test result for similar boats or evaluation through numerical calculations. This study investigated the anti-rolling effect of the stern sub-body in a 50-knot doss planning boat. To carry out this work, new model test procedures were set up in the actual sea. Using this method, the anti-rolling effect of the stern sub-body was investigated. A stern sub-body attached to a planning boat was proved to be effective in reducing the roll and pitch angle.

• 물과 미래
• /
• 제18권1호
• /
• pp.75-83
• /
• 1985

초기연구단계에 있는 ARMA(1, 1) 다계절모형에 의해 계절유량을 발생시키기 위한 모형의 변수결정방법과 유량발생 및 발생유량계열의 통계학적분석을 실시하였으며 타모형과의 비교를 위해 Thomas-Fiering 모형, Matalas AR(1) 다계절모형도 사용하였다. 다계절모형에 의해 발생시킨 계절유량을 연도별로 합산하여 얻은 연유량계열의 통계학적 특성치를 년모의발생모형에 의해 발생시킨 년유량계열의 통계특성치와 비교함으로써 ARMA(1, 1) 다계절모형에 의해 계절 및 년유량자료계열을 한꺼번에 모의발생시킬 수 있는 가능성을 평가하였다.

• 한국자동차공학회논문집
• /
• 제5권2호
• /
• pp.136-148
• /
• 1997

The present study has been carried out to develop a computational procedure for the analysis of the off-design performance in centrifugal compressors with vaneless diffusers by integrating empirical loss models and analytical equations. Losses in centrifugal compressors stem from a number of sources and their exact calculation is not yet possible. This study investigates several modeling schemes and shows that a fairly good prediction can be achieved by a proper selection of the most important flow parameters resulting form a meanline one-dimensional analysis. The performance maps for compressors are calculated and compared with measured performance maps. The off-design performance characteristics in terms of the pressure ratio vs. mass flow produced have generally correct forms. However, no universal means have been found to predict accurately the onset of surge. The prediction method developed through this study can serve as a tool to ensure good matching between parts and it can assist the understanding of the operational characteristics of general purpose centrifugal compressors.

• 디자인학연구
• /
• 제16권1호
• /
• pp.199-208
• /
• 2003

The Israel-born Ron Arad is one of Britain's "superstar" designers, an it has been said that he is Britain's answer to Philippe Starck. Arad is a highly individualistic designer whose ideas stem from a mental process that has more in common with that of the fine artist than of the jobbing designer for industry. His imagination expresses itself in the form of things. By the early 1990s he had become internationally recognized for his "ready-made" work. His furniture and lighting designs required extremely costly labour-intensive techniques to produce. As his work was highly evocative, suggesting a post-industrial world in which the urban landscape was characterized by materials in a state of decay. More recently, through collaborations with Italian and German manufactures, his work has reached a new level of sophistication and finish, which differentiates it from the earlier designs. As Arad reputation as a designer of workshop-based furniture came to an end and the era of "democratic" Arad furniture came into being. By middle of the decade he had become one of the most-discussed designers of the day. The spirituality that emanates from all his work is a product of his particular vision of the creative process, far removed from the typical, stereotyped trends in the sphere of modern industrialization. He goes one step further in his work by attempting to restore aesthetic dignity to the objects of daily life, in a search for beauty within the immediate environment.hin the immediate environment.

• 한국식품영양학회지
• /
• 제19권3호
• /
• pp.267-270
• /
• 2006

한국에서 옻나무(Rhus verniciflua)는 전통적으로 약용식물로 오랫동안 사용되어 왔다. 민간요법으로 위장병 치료를 목적으로 옻나무껍질을 닭과 함께 넣어 끊여서 백숙형태로 옻닭으로 식용하였다. 그러나 일부 사람에게서 피부발진이 발생하는 극심한 알러지 반응을 일으키는 나무로 인식되어 왔다. 알러지 발생을 유발시키는 우루시올은 옻나무의 수지상 옻칠 액의 주성분이다. 이 성분의 화학적 구조는 카테친의 기본구조에 알킬기인 불포화지방산(C$_{15}_{\sim}_{17}$)이 곁사슬로 붙어 있다. 곁사슬의 불포화지방산 성분은 칠기의 고분자화 및 경화과정에 중요한 역할을 하며, 옻칠공예품의 색상 및 품질에 커다란 영향을 미친다. 한국에서 생산되는 원주산 옻칠액의 우루시올성분은 역상 컬럼을 사용하여 고속 액체 크로마토그라피 (HPLC)법으로 정제하여 ESI(-), FAB(+) 질량분석기(MS)로 분석한 결과 분자량 340인 Hetadecatetetraenyl catechol 이었다.

• 대한두경부종양학회지
• /
• 제28권1호
• /
• pp.34-36
• /
• 2012

In case of lateral cervical cystic lesions, the differential diagnoses include branchial cleft cyst(BCC), teratoma, dermoid, hemangioma and lymphangioma etc. But sometimes metastatic cystic lymph nodes may exist in lateral neck. In such circumstance, the primary lesions are known to stem from oropharynx, nasopharynx, salivary and thyroid gland etc. A-66-year-old-male came to our clinic, due to the lateral cervical mass for 5 years. We performed the neck CT, sonography and sono-guided FNAC. He was initially diagnosed with the benign cyst such as BCC. We performed the excisional biopsy on left level II, but the pathologic report was revealed as metastatic papillary thyroid carcinoma(PTC). And then he received the total thyroidectomy with neck dissection. The final diagnosis was cystic metastasis from PTC. We learn a valuable lesson form this case in the following. Even if the simple cervical cyst is presumed with radiology and clinical pattern, more careful considerations on the basis of history and radiologic findings are mandatory.

• Reproductive and Developmental Biology
• /
• 제29권4호
• /
• pp.259-267
• /
• 2005

The present study was conducted to investigate gelatinolytic activities in HAM and to determine whether there are any changes in gelatinolytic activity profiles when the cells are cultured in hepatogenic medium. Placenta was obtained during caesarean section of the volunteers, with informed consent. HAM were isolated from amniotic membrane using collagenase type A HAM were cultured in hepatogenic medium for 3 weeks and the conditioned media were obtained at day 7, 14 and 21. The zymographic pattern of gelatinolytic activity of the HAM did not undergo a change during passages. When the HAM were cultured in a fibronectin-coated dishes in a hepatogenic medium, there was no significant difference of the gelatinase pattern between before and after culture. However, when bFGF was added to the culture, a dramatic increase of 62kDa and 59kDa gelatinases was observed. Interestingly, when ITS instead of FN was present, HAM-conditioned medium also showed a similar increase of both gelatinases. Immunoblotting analysis demonstrated that both 62kDa and 59kDa gelatinases were the active form of MMP-2 resulting from the turnover of MMP-2 proform. Futher study will be necessary to determine the relationship between bFGF and active MMP-2 during hepatogenesis of HAM.