• Korean journal of applied entomology
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• v.41 no.3
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• pp.217-223
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• 2002

The chestnut brown chafer, Adoretus tenuimaculatus Waterhouse, is serious insect pests in golf courses. Adults feed on the leaves of latifoliate trees but larvae feed on roots of turfgrases such as bentgrass, Agrostis spp. Damage of A. tenuimaculatus larvae was observed at the Jinju golf club which showed damage symptom on perennial ryegrass, Lolium perenne in tees and fairways in July, 2000. Damaged turf by A. tenuimaculatus larvae became yellowish and wilted. Symptom of laval damage of A. tenuimaculatus was similar to summer depression in warm season turfgrasses but not recovered by irrigation when Korean isolates of entomopathogenic nematodes were evaluated for the control of A. tenuimaculatus larvae in laboratory and field as a possible biological control agent. The nematodes used were Heterorhabditis bacteriophora Jeju strain, Hererorhabditis sp. Gyeongsan strain, Steinernema carpocapsae Pocheon strain, S.glaseri Dongrea strain, and S.longicaudum Nonsan strain. In the laboratory test H.bacreriophora Jeju strain and Heterorhabditis sp. Gyeongsan strain were highly effective for 3rd instars with 95% mortality. In the field test reduction rates of A.tenuimaculatus larvae were higher by ranging from 28 to 57% by H. bacteriophora Jeju strain, Heterorhabditis sp. Gyeongsan strain, and S.carpocapsae Pocheon strain compared to 7% by natural cause.

• The Korean Journal of Pesticide Science
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• v.20 no.4
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• pp.361-368
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• 2016

Japanese pine sawyer (JPS), Monochamus alternatus is a vector insect of pine wood nematode, Bersaphlenchus xylophilus in Korea and Japanese pine forest. This study was to evaluate the possibility of biological control of JPS with Korean entomopathogenic nematodes (EPNs). Korean EPNs (Heterorhabditis sp. Gyeongsan, Steinernema carpocapsae GSN1, S. glaseri Dongrae and S. longicaudum Nonsan strain) were infested bate insect, Galleria mellonella in log of dead pine tree. Result showed that, S. carpocapsae GSN1 strain has the highest pathogenicity. Heterorhabditis sp. Gyeongsan and S. carpocapsae GSN1 had infested bate insect, which located in 7.5 cm depth of pine log. EPN has pathogenicity against larva and adult of JPS. Spray application of EPNs against adult of JPS, Heterorhabditis sp. Gyeongsan strain showed higher pathogenicity than S. carpocapsae GSN1 strain. EPN infested larva of JPS was detected on bark spray treatment with S. carpocapsae GSN1 strain in dead pine log that naturally infested larva of JPS however, no EPN infested JPS was detected on soaking of pine log with suspension of S. carpocapsae GSN1 strain. Though EPN had found with low efficacy against JPS but still possible to apply to control the boring insect pest as they can control log dwelling insect.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.312-317
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• 2013

The armyworm, Pseudaletia separata was occurred suddenly in a golf club in Namhae, Gyeongnam province in 2013. Thus, pathogenicity of seven species of entomopathogenic nematodes (Steinernema carpocapsae GSN1 strain, S. glaseri Dongrae strain, S. longicaudum Nonsan strain, S. monticolum Jiri strain and S. siamkayai, Heterorhabditis sp. Gyeongsan strain, and H. bacteriophora Hamyang strain) was evaluated against armyworm on tall fescue in pot and golf course to control this pest environmentally friendly. The pathogenicity against P. separata larvae was significantly different depending on nematode species. The corrected mortality of fifth instar of P. separata was 100% in the treatment with S. carpocapsae GSN1 in 7 days in tall fescue pot. However, there was not different in the mortality of fifth instar of P. separata (80 to 100%) at the rate of 385 to 6,160 infective juveniles (Ijs) ($=2.5{\times}10^2$ to $4{\times}10^5Ijs/m^2$) of S. carpocapsae GSN1 strain in each pot. Corrected mortality of P. separata was 65 and 60% at the rate of $10^5Ijs/m^2$ of S. carpocapsae GSN1 and S. longicaudum Nonsan strain, respectively in the tall fescue of golf course.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.171-179
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• 2000

- Turfgrass insect pests and natura.l enemies for biological control were investigated to develop pest management effectively in golf courses at several golf clubs. Twenty eight insect pest species of 10 families in 6 orders were collected from golf courses. The zoysiagrass mite, Eriophyes zoysiae and root-knot nematode, Meloidogyne incognita were also collected from zoysiagrass. White grubs of several scarab beetles and cutworms (Agrotis spp.) damaged seriously at most surveyed golf clubs. In addition, bluegrass webworm (Crambus sp.), Japanese lawngrass cutworm (Spodoptera depravata), scale insects, Tipula sp., and ants (Camponitus japonicus, Formica japonica, and Lasins japonicus) damaged turfgrasses directly or indirectly in golf courses. The entomopathogenic nematodes, Heterorhabditis spp., Steinernema glaseri, and S. longicaudum, entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, and milky disease, Paenibacil/us popil/iae were isolated from white grubs or turfgrass soil as microbial control agents. Besides, dipteran predators, Cophinopoda chinensis, Philonicus albiceps, and Promachus yesonicus and hymenopteran parasitoid, Tiphia sp. were also collected. The P. yesonicus was the most active in golf courses. The root-knot nematode, M. incognita was found from Zoysia japonica, Z. matrella. and Cynodon dactylon.

• Korean journal of applied entomology
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• v.44 no.2
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• pp.145-150
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• 2005

Three Korean isolates of entomopathogenic nematodes, Steinernema carpocapsae KCTC 0981BP (ScK), S. glaseri Dongrae (SgD), and Heterorhabditis sp. KCTC 0991BP (HsK), were evaluated for the control of a turfgrass pest, Popillia quadriguttata. Three days after treatment, all nematodes showed high pathogenicity to adult P. quadriguttata with $97.5\%$ mortality by ScK, $90.8\%$ by HsK, and $80\%$ by SgD at the concentration of 900 infective juveniles per adult. Nematode attachment and infection rate to adult P. quadriguttata were various depending on nematode species and inoculation density. The rate of nematode attachment was $90.8\%$ in HsK, $90.6\%$ in SgD, and $35\%$ in ScK, resfectively at the concentration of 900 infective juveniles per adult. The infection rate that represents the rate of detected nematode from inside insect of body was $97.5\%$ in ScK, $ 80\%$ in both HsK and SgD at the 900 concentration of infected juveniles. The infection rate, however, was decreased to $27.5\%$ in ScK, $72.5\%$ in SgD, but no nematodes was detected in HsK at the concentration of 90 infective juveniles.

• Journal of agriculture & life science
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• v.45 no.6
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• pp.115-124
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• 2011

This study was conducted to find a useful alternative herbivore system with which to study the effects of ultraviolet exposure on the pathogenicity and survival of Korean isolated entomopathogenic nematodes (Heterorhabditis sp. 202 strain, Heterorhabditis sp. Gyeongsan strain, Steinernema sp. 223 strain, S. carpocapsae Pocheon strain, S. glaseri Dongrae strain and S. longicaudum Nonsan strain). Pathogenicity of entomopathogenic nematodes against silkworm, Bombyx mori, differed depending on the nematode species and strain and instar of silkworm challenged. Steinernematidae were relatively more pathogenic to the 5th instar silkworms, whereas Heterorhabditidae were more pathogenic to the 1st instars. UV-C exposure was harmful to entomopathogenic nematodes. All nematodes were killed within 60 minutes of exposure of UV-C, and after 10 min of exposure they caused only 6.7% corrected mortality of silkworms. Silkworms were not infected when they were fed S. carpocapsae Pocheon strain treated mulberry leaves which were exposed to UV intensity of about $2.3mW/cm^2$ for 4 hours in the field. Pathogenicity of S. carpocapsae was not significantly reduced after 1 hour of exposure to $4.0mW/cm^2$ UV intensity on the mulberry leaves against silkworms.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.245-252
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• 2001

Entomopathogenic nematodes were effective in the control of the cotton caterpillar, Palpita indica Saunder. P. indica mortality was significantly different depending on nematode species, treatment concentration, and instar. S. carpocapsae Pocheon strain was more effective against P. indica than S. glaseri Dongrae strain, S. longicaudum Nonsan strain, Heterorhabditis sp. Gyeongsan isolate, and H. bacteriophora Hamyang strain. When S. carpocapsae was treated with the rate of > 20 infective juveniles (ijs)/larva, mortality was ca. 100% at the 1st-4th instars of P. indica in 72h. $LC_{50}$ of entomopathogenic nematodes were significantly different depending on nematode species. The lowest $LC_{ 50}$ value was obtained by S. carpocapsae with 4.9-8.2 ijs in the 1st-prepupa while the highest $LC_{50}$ by Heterorhabditis sp. with 5.5-21.9 ijs, the shortest LT$_{50}$ value of P. indica was induced by S. carpocapsae when 20 ijs/larva were inoculated. The $LT_{50}$ s were 3.4-9.2h against the 1st-prepupa. The mortality of P. indica in fields was significantly different depending on field condition, nematode concentration, and leaf location. Mortality of P. indica by S. carpocapsae was higher at greenhouse,$ 3$\times$10^{9}$ ijs/ha and upper leaves than at field, 1$\times$10$^{9}$ ijs/ha and lower and middle leaves, respectively.

• Korean journal of applied entomology
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• v.43 no.1
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• pp.61-70
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• 2004

Environmentally sound control of Japanese lawngrass cutworm, Spodoptera depravata with Korean entomopathogenic nematodes (Heterorhabditis sp. Gyeongsan, Steinernema carpocapsae GSNI , S. glaseri Dongrae, S. longicaudum Nonsan, and S. monticolum Jiri) was evaluated in the laboratory and field. In addition, effect of turf height on the pathogenicity of entomopathogenic nematodes and density of ant (Formica japonica) and spine-tailed earwigs (Forficula scudderi) were investigated in the field. The corrected mortality of 3rd instar of S. depravata larvae was 53.3% to 66.7% 5 days later after treatment of entomopathogenic nematodes. $LC_{50}$ value of S. carpocapsae GSN1 and S. monticolum Jiri against 3rd instar of S. depravata was 6.9 and 3.9, respectively. Pathogenicity of S. carpocapsae GSN1 was different depending on larval stages, i.e., mortality of 2nd instar was the highest representing 73.3% but decreased from 3rd instar. Turf height affected pathogenicity of entomopathogenic nematodes. Mortality of 4th instar of S. depravata was 40.0% by S. carpocapsae GSN1 and 33.3% by S. monticolum Jiri, and 83.3% by fenitrothion, respectively, in the turf height of 14mm, but those were lower in 45mm. The corrected mortality of S. depravata larvae was lower at the entomopathgenic nematode plots than fenitrothion plot in the distribution field of Formica japonica. However, reduction rate of F japonica was higher in fenitrothion by 56.7% compared with 0% in S. carpocapsae GSN1 and 6.7% in S. monticolum Jiri. Turf height influenced control of S. depravate in S. carpocapsae GSN1 and fenitrothion. Control value of S. carpocapsae GSN1 was 62.2% in the turf height of 3-4cm, but not effective at all in 6-8cm and 12-14cm. However, there was not significantly different in fenitrothion plots. Forficula Scudderi was also reduced only in fenitrothion plots. Reduction rate was 100% in 3-4cm, 41.7% in 6-8cm, and 16.7% in 12-14cm, respectively.