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Effect of Ultraviolet Light on Survival and Pathogenicity of Entomopathogenic Nematodes against an Alternative Host Silk Worm, Bombyx mori  

Lee, Dong-Woon (School of Ecological Environment and Tourism, Kyungpook National Univ.)
Kim, Young-Sub (School of Ecological Environment and Tourism, Kyungpook National Univ.)
Kim, Pan-Gi (School of Ecology and Environmental System, Kyungpook National Univ.)
Choo, Ho-Yul (Dept. of Applied Biology, Gyeongsang National Univ.(Insti. of Agric. & Life Sci.))
Publication Information
Journal of agriculture & life science / v.45, no.6, 2011 , pp. 115-124 More about this Journal
Abstract
This study was conducted to find a useful alternative herbivore system with which to study the effects of ultraviolet exposure on the pathogenicity and survival of Korean isolated entomopathogenic nematodes (Heterorhabditis sp. 202 strain, Heterorhabditis sp. Gyeongsan strain, Steinernema sp. 223 strain, S. carpocapsae Pocheon strain, S. glaseri Dongrae strain and S. longicaudum Nonsan strain). Pathogenicity of entomopathogenic nematodes against silkworm, Bombyx mori, differed depending on the nematode species and strain and instar of silkworm challenged. Steinernematidae were relatively more pathogenic to the 5th instar silkworms, whereas Heterorhabditidae were more pathogenic to the 1st instars. UV-C exposure was harmful to entomopathogenic nematodes. All nematodes were killed within 60 minutes of exposure of UV-C, and after 10 min of exposure they caused only 6.7% corrected mortality of silkworms. Silkworms were not infected when they were fed S. carpocapsae Pocheon strain treated mulberry leaves which were exposed to UV intensity of about $2.3mW/cm^2$ for 4 hours in the field. Pathogenicity of S. carpocapsae was not significantly reduced after 1 hour of exposure to $4.0mW/cm^2$ UV intensity on the mulberry leaves against silkworms.
Keywords
Entomopathogenic nematode; Silkworm; Pathogenicity; Survival; Ultraviolet light;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Abbas, M. S. T., M. M. E. Saleh, and A. M. Akil. 2001. Laboratory and field evaluation of the pathogenicity of entomopathogenic nematodes to the red palm weevil, Rhynchophorus ferrugineus (Oliv.) (Col.:Curculionidae). J. Pest Science 74: 167-168.
2 Akhurst, R. J. and N. E. Boemare. 1990. Biology and taxonomy of Xenorabdus. In : Gaugler, R. and Kaya, H. K. (Eds.), Entomopathogenic nematodes in biological control. CRC Press, Boca Raton.
3 Bedding, R. A., and R. J. Akhurst. 1975. A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21: 109-110.   DOI
4 Begley, J. W. 1990. Efficacy against insects in habitats other than soil. In : Gaugler, R. and Kaya, H. K. (Eds.), Entomopathogenic nematodes in biological control. CRC Press, Boca Raton. 215-231.
5 Choo, H. Y., Kaya, H. K. and S. P. Stock. 1995. Isolation of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Korea. Jap. J. Nematol. 25: 44-51.
6 Choo, H. Y., Lee, D. W., Yun, H. S., Lee, S. M., and D. T. Hang. 2002. Effect of temperature and nematode concentration on pathogenicity and reproduction of entomopathogenic nematode, Steinernema carpocapsae Pocheon strain (Nematoda: Steinernematidae). Korean J. Appl. Entomol. 41: 269-277.
7 Dowds, B. C. A., and A. Peters. 2002. Virulence mechanisms. In : Gaugler, R. (Ed.), Entomopathogenic nematology. CABI Publishing. New York. 79-98.
8 Dutky, S. R., J. V. Thompson, and G. E. Cantwell. 1964. A technique for the mass propagation of the DD-136 nematode. J. Insect Pathol. 6: 417-422.
9 Ebssa, L., C. Borgemeister and H. -M. Poehling. 2003. Effectiveness of different species/strains of entomopathogenic nematodes for control of western flower thrips (Franklini ellaoccidentalis) at various concentrations, host densities, and temperatures. Biological Control 29: 145-154.
10 Fujiie, A., and T. Yokoyama. 1998. Effects of ultraviolet light on the entomopathogenic nematode, Steinernema kushidai and its symbiotic bacterium, Xenorhabdus japonicus. Appl. Entomol. Zool. 33: 263-269.
11 Gaugler, R., A. Bednarek and J. F. Campbell. 1992. Ultraviolet inactivation of heterorhabditid and steinernematid nematodes. J. Invertebr. Pathol. 59: 155-160.   DOI
12 Gaugler, R., and G. M. Boush. 1978. Effects of ultraviolet radiation and sunlight on the entomogenous nematode, Neoaplectana carpocapsae. J. Invertebr. Pathol. 32: 291-296.   DOI
13 Gaugler, R., and G. M. Boush. 1979. Laboratory tests on ultraviolet protectants of an entomopathogenic nematode. Environ. Entomol. 8: 810-813.
14 Georgis, R. and Manweiler, S. A. 1994. Entomopathogenic nematodes: a developing biological control technology. In : Evans, K. (Ed.), Agricultural Zoology Reviews. Andover, Intercept, 63-94.
15 Glazer, I. 1992. Invation rate as a measure of infectivity of steinernematid and heterorhabditid nematodes to insects. J. Invertebr. Pathol. 59: 90-94.   DOI
16 Han, S. M., and M. S. Han. 1999. Isolating entomopahogenic nematode in South Korea. Korean J. Ecol. 22: 255-263.
17 Hang, D. T., Choo, H. Y., Lee, D. W., Lee, S. M., Kaya, H. K., and C. G. Park. 2007. Temperature effects on Korean entomopathogenic nematodes, Steinernema glaseri and S. longicaudum and their symbioticbacteria. Journal of Microbiology and Biotechnology 17: 420-427.
18 Kaya, H. K. and R. Gaugler. 1993. Entomopathogenic nematodes. Annu. Rev. Entomol. 38: 181-206.   DOI   ScienceOn
19 Kaya, H.K. and S. P. Stock. 1997. Techniques in insect nematology. In : Lacey, L. A. (Ed.), Manual of techniques in insect pathology. Academic Press, London, UK, 281-324.
20 Kim, H. H., H. Y. Jeon, S. R. Cho, D. W. Lee and H. Y. Choo. 2006a. Persistence of entomopathogenic nematode, Steinernema carpovapsae GSN1 strain on vegetable leaf in greenhouses. Kor. J. Hort. Sci. Technol. 24: 198-204.
21 Kim, H. H., S. R. Cho, D. W. Lee, H. Y. Jeon, C. G. Park and H. Y. Choo. 2006b. Biological control of diamondback moth, Plutella xylostella with Korean isolates of entomopathogenic nematodes (Steinernematid and Heterorhabditid) in greenhouse. Korean J. Appl. Entomol. 45: 201-209.
22 Kim, H. H., S. R. Cho, D. W. Lee, S. M. Lee and H. Y. Choo. 2006c. Biological control of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae) with entomopathogenic nematodes (Steinernematid and Heterorhabditid) in greenhouse. The Korean Journal of Pesticide Science 10: 335-343.
23 Kim, H. H., S. R. Cho, H. Y. Choo, S. M. Lee, H. Y. Jeon and D. W. Lee. 2008. Biological control of tabacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae) by Steinernematid and Heterorhabditid entomopathogenic nematodes. Korean J. Appl. Entomol. 47: 447-456.   DOI
24 Klein, M. G. 1990. Efficacy against soil-inhabiting insect pests. In : Gaugler, R. and Kaya, H. K. (Eds.), Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, 195-214.
25 Lee, D. W., J. Y. Yang, G. Y. Han, H. Y. Choo, S. M. Lee, H. H. Kim, C. G. Park and Y. M. Choo. 2006. Entomopathogenic nematodes for biological control of Pryeria sinica Moore (Lepidoptera: Zygaenidae) and persistence on Euonymus japonica Thunberg foliage. J Asia-Pacific Entomol. 9: 165-172.   DOI   ScienceOn
26 Lee, H. W., and K. W. Woo. 1980. Mulberry tree protection. Hakmunsa, Seoul, 325pp.
27 Lee, S. W., D. W. Lee and H. Y. Choo. 2007. Development of economical artificial diets for greater wax moth, Galleria mellonella (L.). Korean J. Appl. Entomol. 46: 385-392.   DOI
28 Mannion, C. M., and R. K. Jansson. 1993. Infectivity of five entomopathogenic nematodes to the sweetpotato weevil, Cylas formicarius (F.),(Coleoptera: Apionidae) in three experimental arenas. J. Invertebr. Pathol. 62: 29-36.   DOI   ScienceOn
29 Molles, M. C. Jr. 2008. Ecology: concepts and applications. The McGraw-Hill Companies, Inc. 574pp.
30 Nam, J. H. and Y. I. Ma. 2000. Insect resources and utilization in various country. Seoul National University Press, Seoul, 206pp.
31 Speiser, B., J. G. Zaller and A. Neudecker. 2001. Size-specific susceptibility of the pest slugs Derocera sreticulatum and Arion lusitanicus to the nematode biocontrol agent Phasmarhabditis hermaphrodita. BioContro 146: 311-320.
32 Nickle, W. R., and M. Shapiro. 1994. Effects of eight brighteners as solar radiation protectants for Steinernema carpocapsae, Allstrain. Supplement to Journal of Nematology 26: 782-784.
33 Poinar, G. O., Jr. 1979. Nematodes for biological control of insects. CRC Press, Boca Raton, Florida, USA.
34 SAS Institute. 1996. "SAS 6.11 for Windows" SAS Institute, Cary, NC.
35 Stock, P. S., Choo, H. Y., and Kaya, H. K. 1997. First record of Steinernema glaseri Steiner,1929 (Nematoda: Steinernematidae) in Asia, with notes on intraspecific variation. Nematologica 43: 377-381.   DOI   ScienceOn
36 Tanada, Y., and H. K. Kaya. 1993. Insect pathology. Academic Press, San Diego.
37 Thomson, W. T. 1992. A worldwide guide to beneficial animals used for pest control purposes. Thomson publications, Fresno, 91pp.
38 Woodring, J. L., Kaya, H. K. 1988. Steinernematid and heterorhabditid nematode: a handbook of techniques. South. Coop. Ser. Bull. Arkans. Agric. Exp. STN. Fayetteville, 331: 1-30.