• Bulletin of the Korean Chemical Society
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• v.19 no.3
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• pp.289-290
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• 1998

• Bulletin of the Korean Chemical Society
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• v.10 no.3
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• pp.328-329
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• 1989

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.518-521
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• 2004

Ginseng saponin was isolated from panax ginseng root using high-speed countercurrent chromatography (HSCCC). Preliminary studies were performed to optimize physical properties of two-phase solvent system and operating parameters including rotation speed of column, elution mode of mobile phase, and flow rate. Two-phase solvent system for isolation of ginseng saponins was composed of chloroform, water, and methanol as blending solvent. Chloroform-aqueous methanol (4:6) systems with various concentration of methanol in water were evaluated for retention of stationary phase in column. Retention of stationary phase decreased with increasing flow rate in tail-to-head elution mode using upper phase as mobile phase and head-to-tail elution mode using lower phase as mobile phase. Latter mode produced high retention at flow rate of 5 mL/min. Optimum conditions for isolation of saponin were chloroform/methanol/water (40/39/21) solvent system; mobile phase, of lower organic layer, flow rate, of 5 mL/min, head to tail elution mode, rotation speed, of 800 rpm, and sample injection, of $200{\mu}L$, Recovery yield of ginseng saponin from panax ginseng root extract by HSCCC was 63.6%, and the purity of HSCCC fractions was verified by TLC.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.5
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• pp.1310-1321
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• 1994

Turbulent coherent structures in the intermediate wake of a stationary and rotating cylinder, spin rate S=0.7, situated in a uniform were experimentally investigated using a conditionalphase average technique. Measurements were carried out at a section of 8.5 diameters downstream form the center of cylinder and a Reynolds number of $Re=6.5{\times}10^{3}.$/TEX> The phase averaged velocity and velocity vector fields, contours of vorticity, turbulent intermittency function and velocity fluctuation energy are presented and discussed in relation to the large scale coherent structures by Karman vortices that shed periodically from the cylinder. Coherent wake structures of the rotating cylinder is almost identical with stationary cylinder, but the lateral displacement and shrinkage of turbulent wake region is occured by rotation. Rotation of the cylinder result in that the deflection of wake center to deceleration region(Y/D${\simeq}-0.3)$ and the decrease of mean velocity defect(10%), vorticity strength of large scale structures(19%), total velocity fluctuation energy(12%).

• Journal of Power Electronics
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• v.15 no.4
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• pp.994-1005
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• 2015

This paper presents a novel power control strategy for PWM current-source rectifiers (CSRs) in the stationary frame based on the instantaneous power theory. In the proposed control strategy, a virtual resistance based on the capacitor voltage feedback is used to realize the active damping. In addition, the proportional resonant (PR) controller under the two-phase stationary coordinate is designed to track the ac reference current and to avoid the strong coupling brought about by the coordinate transformation. The limitations on improving steady-state performance of the PR controller is investigated and mitigated using a cascaded lead-lag compensator. In the z-domain, a straightforward procedure is developed to analyze and design the control-loop with the help of MATLAB/SISO software tools. In addition, robustness against parameter variations is analyzed. Finally, simulation and experimental results verify the proposed control scheme and design method.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1529-1536
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• 2008

Acetylation of lysine residues in proteins is a reversible and highly regulated posttranslational modification. However, it has not been systematically studied in prokaryotes. By affinity immunoseparation using an anti-acetyllysine antibody together with nano-HPLC/MS/MS, we identified 125 lysine-acetylated sites in 85 proteins among proteins derived from Escherichia coli. The lysine-acetylated proteins identified are involved in diverse cellular functions including protein synthesis, carbohydrate metabolism, the TCA cycle, nucleotide and amino acid metabolism, chaperones, and transcription. Interestingly, we found a higher level of acetylation during the stationary phase than in the exponential phase; proteins acetylated during the stationary phase were immediately deacetylated when the cells were transferred to fresh LB culture medium. These results demonstrate that lysine acetylation is abundant in E. coli and might be involved in modifying or regulating the activities of various enzymes involved in critical metabolic processes and the synthesis of building blocks in response to environmental changes.

• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.939-945
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• 2006

The physiological characteristics and function of the exopolysaccharide (EPS) produced by Lactobacillus rhamnosus ATCC 9595 were determined. The total quantity of EPS was rapidly increased to 496$\pm$20 mg/l during the exponential phase, and then maintained steadily during the stationary phase. During the exponential phase (18 h), the total EPS consisted of 61% cell-bound EPS (cb-EPS) and 39% released EPS (r-EPS), whereas the relative proportion of EPS during the stationary phase (48 h) was convered to 23% cb-EPS and 77% r-EPS. On gel permeation chromatography, cb-EPS was fractionated as a single peak of 8.6$\times10^6$ Da, whereas r-EPS was fractionated as two peaks with average molecular weights of 4.3$\times$10$^4$ and 8.6$\times10^6$ Da. Interestingly, both EPS species exhibited anticancer properties and cholera toxin-binding activities. Our results suggest that the EPS generated by L. rhamnosus ATCC 9595 might be suitable for use as a functional food or food supplement.

• KSBB Journal
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• v.22 no.2
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• pp.119-122
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• 2007

Since egg white contains various protein, it is important to research the protein distribution of egg white. Specially, lysozyme and ovalbumin important proteins, are used in medicine and food industry. Reversed phase high performance liquid chromatography has been used for separation of egg white, and column of RP-HPLC is available in variety. We have used C4, C8 and C18 columns to obtain chromatograms by varying carbon chain length of stationary phase. Long carbon chain length of stationary phase has good separation of egg white. Also, we have changed the composition of mobile phase (acetonitrile, water, and trifluoroacetic acid) to find optimum chromatograms. Acetonitrile and water composition of 50 : 50 show many peaks from egg white. Isocartic and gradient elution in RP-HPLC were used to compare the chromatography of egg white.

• Bulletin of the Korean Chemical Society
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• v.26 no.5
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• pp.823-825
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• 2005

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3497-3500
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• 2012