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HPLC Study for Egg White Analysis  

Jeon, Young-Ju (Department of Chemical engineering, Chungnam National University)
Lee, Eun (Department of Chemical engineering, Chungnam National University)
Kim, In-Ho (Department of Chemical engineering, Chungnam National University)
Publication Information
KSBB Journal / v.22, no.2, 2007 , pp. 119-122 More about this Journal
Abstract
Since egg white contains various protein, it is important to research the protein distribution of egg white. Specially, lysozyme and ovalbumin important proteins, are used in medicine and food industry. Reversed phase high performance liquid chromatography has been used for separation of egg white, and column of RP-HPLC is available in variety. We have used C4, C8 and C18 columns to obtain chromatograms by varying carbon chain length of stationary phase. Long carbon chain length of stationary phase has good separation of egg white. Also, we have changed the composition of mobile phase (acetonitrile, water, and trifluoroacetic acid) to find optimum chromatograms. Acetonitrile and water composition of 50 : 50 show many peaks from egg white. Isocartic and gradient elution in RP-HPLC were used to compare the chromatography of egg white.
Keywords
RP-HPLC; gradient elution system; egg white;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Kim, W. K. and B. H. Chung (1999), Optimization of chromatographic separation of lysozyme from homogenate of hen egg white by comparison of breakthough behavior, Korean J. Biotechnol. Bioeng. 14(3), 279-283   과학기술학회마을
2 Kim, H. W. and I. H. Kim (2003), Comparison lysozyme purification from egg white between ion exchange chromatography and precipitation, HWAK KONGHAK 41(3), 332-336
3 Huntingston, J. A. and P. Stein (2001), Structure and properties of ovalbumin, J. Chromatoar. B 756, 189-198   DOI   ScienceOn
4 Guiochon, G., Golshan-Shirazi, A .M, S. Katti (1994), Fundamentals of preparative and nonlinear chromatography, P393-397, Academic Press, Boston
5 Lu, J., Y. Wan, and Z. Cui (2006), Strategy to separate lysozyme and ovalbumin from CEW using UF, Desalination, 200, 477-479   DOI   ScienceOn
6 Nau, F., A. Mallard, J. Pages, and G. Brule (1999), Reversed-phase liquid chromatography of egg white proteins. Optimization of ovalbumin elution, J. Liq. Chrom.&ReI. Technol. 22(8), 1129-1147   DOI   ScienceOn
7 Awade, A. C. and T. Efstathiou (1999), Comparison of three liquid chromatographic methods for egg-white protein analysis, J. Chromatoar. B 723, 69-74   DOI   ScienceOn
8 Itoh, H., N. Noriyuki, T. Kinosita, N. Nagae, and N. Mitsugu (1991), Fast protein separation by reversed-phase high-performance liquid chromatography on octadecylsilyl-bonded nonporous silica gel, Anal. Biochem. 199, 7-10   DOI   ScienceOn