• Title/Summary/Keyword: Stationary Phase Method

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Enhanced Phase Angle Detect Method Using High-pass Filter (고주파 필터를 이용한 개선된 위상각 검출 방법)

  • Heo, Min-Ho;Song, Sung-Gun;Kim, Gwang-Heon;Nam, Hae-Gon;Park, Sung-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.58 no.12
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    • pp.2370-2378
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    • 2009
  • The enhanced phase angle estimation algorithm is essential to supply the power stably under synchronizing with grid source. In this paper, we are proposed the novel phase angle estimation algorithm and verified the validity of proposed method as simulation with PSIM and experiments. We sort the harmonics element using high-pass filter(HPF) that have the cut-off frequency below basic element and make reverse d-q transformation. So, it can be restored the harmonics element at stationary axis, and we can get the fundamental voltage element of AC grid. Proposed PLL method have a rapid responsibility and a large margin at controller design than conventional method because it have a small phase delay and a sufficient controller gain margin. And, it can reduce the error of voltage rms value and axis transformation according to robust PLL algorithm against the harmonic and phase unbalance.

Evaluation of interaction between organic solutes and a membrane polymer by an inverse HPLC method

  • Kiso, Yoshiaki;Hosogi, Katsuya;Kamimoto, Yuki;Jung, Yong-Jun
    • Membrane and Water Treatment
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    • v.5 no.3
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    • pp.171-182
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    • 2014
  • Organic compounds are adsorbed on RO/NF membranes, and the adsorption may influence the rejection of organic compounds by the membranes. Because almost RO/NF membranes are composite membranes, the results obtained by adsorption experiment with using membrane pieces are unable to avoid the influence by the support membrane. In this work, the interaction between membrane polymer and organic solutes was examined by an inverse HPLC methodology. Poly (m-phenylenetrimesoylate), the constituent of skin layer of RO/NF membranes, was coated on silica gel particles and used as a stationary phase for HPLC. When water was used as a mobile phase, almost hydrophilic aliphatic compounds were not effectively adsorbed on the stationary phase, although hydrophobic compounds were slightly adsorbed. The results indicated that the hydrophilic aliphatic compounds are useful probe solutes to examine the molecular sieving effect of a membrane. When water was used as a mobile phase, the aromatic compounds were strongly retained, and therefore $CH_3CN/H_2O$ (30/70) was used as a mobile phase. It was revealed that the adsorption of aromatic compounds was controlled by stacking between solute and polymer and was hindered by non-planar structure and substituents.

Identification and Partial Characterization of Lacticin SA72, a Bacteriocin Produced by Lactococcus lactis SA72 Isolated from Jeot-gal

  • Koo, Kyoung-Mo;Lee, Na-Kyoung;Hwang, Young-Il;Paik, Hyun-Dong
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.488-495
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    • 2000
  • Strain SA72 was isolated from Jeot-gal and identified as producer of a bacteriocin, which showed some bactericidal activity against Lactobacillus delbrueckii ATCC 4797. Strain SA72 was tentatively identified as Lactococcus lactis according to the AOI test. Lactococcus lactis SA72 showed a broad spectrum of microorganisms, tested by the modified deferred method. The activity of lacticion SA72, named tentatively as a bacteriocin produced by Lactococcus lactis SA72, was detected during the mid-lon growth phase, reached a maximum during the early stationary phase, and then declined after the late stationary phase. Lacticin SA72 also showed a relatively broad spectrum of activity against non-pathogenic and pathogenic microorganisms when assessed by the spot-on-lawn method. Its anitimicrobial activity on sensitive indicator cells disappeared completely by protease XIV treatment. The inhibitory activity of lacticin SA72 remained after treatment for 15 min at $121^{\circ}C$, 문 was stable in a pH range of 2.0 to 9.0 and all organic solvents examined. It demonstrated a typical bactericidal mode of inhibition against Lactobacillus delbrueckii ATCC 4797. The apparent molecular mass of lacticin SA72 was in the region of 3-3.5 kDa, determined by SDS-PAGE.

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A New Control Strategy for a Three-Phase PWM Current-Source Rectifier in the Stationary Frame

  • Guo, Qiang;Liu, Heping;Zhang, Yi
    • Journal of Power Electronics
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    • v.15 no.4
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    • pp.994-1005
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    • 2015
  • This paper presents a novel power control strategy for PWM current-source rectifiers (CSRs) in the stationary frame based on the instantaneous power theory. In the proposed control strategy, a virtual resistance based on the capacitor voltage feedback is used to realize the active damping. In addition, the proportional resonant (PR) controller under the two-phase stationary coordinate is designed to track the ac reference current and to avoid the strong coupling brought about by the coordinate transformation. The limitations on improving steady-state performance of the PR controller is investigated and mitigated using a cascaded lead-lag compensator. In the z-domain, a straightforward procedure is developed to analyze and design the control-loop with the help of MATLAB/SISO software tools. In addition, robustness against parameter variations is analyzed. Finally, simulation and experimental results verify the proposed control scheme and design method.

PWM-VSI controller of Three-phase UPS Using Stationary Reference Frame (정지좌표계를 이용한 3상 UPS용 PWM-VSI 제어)

  • Kim M.K.;Kim J.S.;Bang S.S.;Choi J.H.
    • Proceedings of the KIPE Conference
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    • 2003.07b
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    • pp.965-968
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    • 2003
  • This paper describes the PWM-VSI controller of three-phase UPS system using stationary reference frame. This controller meets the specification the UPS inverter output voltage even under the unbalanced or nonlinear load. This controller is also constructed with duble control loop of the outer voltage control loop and the inner current control loop. For the fast response of the output voltage control, yhr inner current control loop of the capacitor current os used. To get the good property against overshoot, the If controller us used. The outer voltage controller is designed with P controller and the high gain transfer function is used for the zero steady state error. All control gains of both controller is designed base on the CDM method.

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Improving Mycoplasma ovipneumoniae culture medium by a comparative transcriptome method

  • Wang, Xiaohui;Zhang, Wenguang;Hao, Yongqing
    • Journal of Veterinary Science
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    • v.21 no.2
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    • pp.30.1-30.11
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    • 2020
  • Mycoplasma ovipneumoniae (Mo) is difficult to culture, resulting in many difficulties in related research and application. Since nucleotide metabolism is a basic metabolism affects growth, this study conducted a "point-to-point" comparison of the corresponding growth phases between the Mo NM151 strain and the Mycoplasma mycoides subsp. capri (Mmc) PG3 strain. The results showed that the largest difference in nucleotide metabolism was found in the stationary phase. Nucleotide synthesis in PG3 was mostly de novo, while nucleotide synthesis in NM151 was primarily based on salvage synthesis. Compared with PG3, the missing reactions of NM151 referred to the synthesis of deoxythymine monophosphate. We proposed and validated a culture medium with added serine to fill this gap and prolong the stationary phase of NM151. This solved the problem of the fast death of Mo, which is significant for related research and application.

Control Method for Fault-Tolerant Active Power Filters

  • Zhang, Chenyu;Zheng, Jianyong;Mei, Jun;Deng, Kai;Zhou, Fuju
    • Journal of Power Electronics
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    • v.15 no.3
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    • pp.796-805
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    • 2015
  • New direct and indirect current control methods for a fault-tolerant active power filter topology are presented in this paper. Since a three-phase four-switch topology has a phase bridge current which cannot be directly controlled, a hysteresis control method in the α-β plane which controls the three-phase current in the two-phase stationary coordinate system is proposed. The improved SVPWM algorithm is able to eliminate the operation of the trigonometric functions in the traditional algorithm by rotating the α-β coordinates and alternating the sequence of the output vectors, which in turn simplifies the algorithm and reduces the switching frequency. The selection of the DC-side reference voltage and DC-side capacitor equalization strategy are also discussed. Simulation and experiments demonstrate that the proposed control method is correct and feasible.

Enantiomer Separation of Chiral Amino Alcohols as 9-anthraldimine Derivatives on Coated and Covalently Bonded Chiral Stationary Phases Based on Polysaccharide Derivatives by High Performance Liquid Chromatography (고성능 액체 크로마토그래피에 의한 다당 유도체를 기초로 한 흡착되거나 공유결합된 키랄 고정상에서 키랄 아미노 알코올의 안트르알디민 유도체의 광학분리)

  • Xu, Wen Jun;Jin, Jing Yu;Lee, Won-Jae
    • KSBB Journal
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    • v.26 no.4
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    • pp.323-327
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    • 2011
  • The convenient derivatization method of chiral amino alcohols as 9-anthraldimine Schiff base derivatives for chiral resolution was developed and the liquid chromatographic enantiomer separation of chiral amino alcohols as 9-anthraldimine derivatives was investigated on several coated and covalently bonded polysaccharide-derived chiral stationary phases (CSPs). In general, the performance of Chiralcel OD-H (or Chiralcel OD) (${\alpha}$ = 1.24-2.89), the coated CSP derived from cellulose derivative was superior to the other CSPs for resolution of 9-anthraldimine derivatives of several amino alcohols. The results of enantioseparation depending on the structure of 9-anthraldimine analytes like the steric bulky group and the polar moiety etc were discussed. The analytical method was applied to measure the enantiomeric purity of commercially available chiral amino alcohols. It is expected that the convenient analytical method will be very efficient for determination of enantiomeric purity of amino alcohols as 9-anthraldimine Schiff base derivatives with strong UV absorption.

Liquid chromatographic enantioseparation of several amino acids as nitrobenzoxadiazole derivatives on polysaccharide trisphenylcarbamate derived chiral stationary phases

  • Suraj Adhikari;Alisha Bhandari;Wonjae Lee
    • Analytical Science and Technology
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    • v.36 no.4
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    • pp.143-151
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    • 2023
  • Considering the greater role of α-amino acids in our daily lives, the enantiomer resolution of seven α-amino acids derivatized with fluorogenic reagent (4-fluoro-7-nitro-2,1,3-benzoxadiazole, NBD-F) by chiral HPLC on amylose or cellulose trisphenylcarbamate derived chiral stationary phases (CSPs) under simultaneous ultraviolet (UV) and fluorescence (FL) detection was performed. The degree of enantioseparation and resolution was affected by nature and selector backbones of the CSPs as well as the kind of amino acids. Baseline enantiomer separation and resolutions were observed for the enantiomers of all analytes as NBD derivatives especially on coated type amylose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak AD-H and Lux Amylose-1). The other CSPs also showed good enantioselectivity except for the CSPs (Chiralpak IB, Chiralcel OD-H and Lux Cellulose-1) having cellulose tris(3,5-dimethylphenylcarbamate) as chiral selectors. The developed analytical chiral method was applied to determine the enantiomeric purity of seven commercially available L-α-amino acids and the impurities as D-forms were found to be in the range 0.08-0.87 %, respectively. The intra- and interday accuracy and precision assays showed high accuracy and precision of the developed analytical method. This chiral HPLC method for the enantiomer resolution of amino acids using fluorescent derivatization could be useful for the determination of enantiomeric purity of pharmaceuticals and biological study for amino acid type compounds among chiral drugs.

Image Authentication Using Only Partial Phase Information from a Double-Random-Phase-Encrypted Image in the Fresnel Domain

  • Zheng, Jiecai;Li, Xueqing
    • Journal of the Optical Society of Korea
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    • v.19 no.3
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    • pp.241-247
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    • 2015
  • The double-random phase encryption (DRPE) algorithm is a robust technique for image encryption, due to its high speed and encoding a primary image to stationary white noise. Recently it was reported that DRPE in the Fresnel domain can achieve a better avalanche effect than that in Fourier domain, which means DRPE in the Fresnel domain is much safer, to some extent. Consequently, a method based on DRPE in the Fresnel domain would be a good choice. In this paper we present an image-authentication method which uses only partial phase information from a double-random-phase-encrypted image in the Fresnel domain. In this method, only part of the phase information of an image encrypted with DRPE in the Fresnel domain needs to be kept, while other information like amplitude values can be eliminated. Then, with the correct phase keys (we do not consider wavelength and distance as keys here) and a nonlinear correlation algorithm, the encrypted image can be authenticated. Experimental results demonstrate that the encrypted images can be successfully authenticated with this partial phase plus nonlinear correlation technique.