• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1628-1634
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• 2009

Small heat shock proteins (sHSPs) function as molecular chaperones that protect cells against environmental stresses. In the present study, the genes of hsp17.6 and hsp17.7, cytosolic class I sHSPs, were cloned from a tropical plant, Ageratina adenophorum. Their C-terminal domains were highly conserved with those of sHSPs from other plants, indicating the importance of the C-terminal domains for the structure and activity of sHSPs. The recombinant HSP17.6 and HSP17.7 were applied to determine their chaperone function. In vitro, HSP17.6 and HSP17.7 actively participated in the refolding of the model substrate citrate synthase (CS) and effectively prevented the thermal aggregation of CS at $45^{\circ}C$ and the irreversible inactivation of CS at $38^{\circ}C$ at stoichiometric levels. The prior presence of HSP17.7 was assumed to suppress the thermal aggregation of the model substrate CS. Therefore, this report confirms the chaperone activity of HSP17.6 and HSP17.7 and their potential as a protectant for active proteins.

• Journal of the Korean Institute of Intelligent Systems
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• v.12 no.2
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• pp.164-170
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• 2002

Sliding mode control, as a typical method of variable structure control, has the robust characteristics for the uncertainty and the disturbance of the nonlinear system. Because, however, sliding mode control input includes a sign function that Is discontinuous on the predefined switching surface, its applications are primarily limited by the need of alleviation or reduction of chattering. In this paper, we propose a chattering alleviation strategy based on a special nonlinear function and a fuzzy system. By using the proposed control scheme, we can reduce the steady state error. Its tracking performance is as fast as that of conventional method using the fixed boundary layer. Especially, in the proposed method, we can adjust the trade-off between the steady state error and the degree of chattering by regulating the proper range of the output variable of the fuzzy system. To verify the validity of the proposed algorithm, the analysis of the control method using the fixed boundary layer and the computer simulations are shown to compare with them.

• Archives of Pharmacal Research
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• v.27 no.11
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• pp.1177-1182
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• 2004

The effect of poly[2-methacryloyloxyethyl phosphorylcholine] (pMPC) on the skin permeation property was investigated by performing in vitro skin permeation study of a model drug, nicotinic acid (NA). Effect of pMPC polymer in donor solution on skin permeation rates was evaluated using side-by-side diffusion cells. Also, the structural alterations in the stratum corneum (SC), inter-lamellar bilayer (ILB) and dermis layers in pMPC-treated and -untreated skin sections were investigated with transmission electron microscopy (TEM). The permeation profile of NA without pMPC in donor solution showed biphasic mode: initial $1^{st} phase and 2^{nd}$ hydration phase. The sudden, more than 10-fold increase in flux from the initial steady state (43.5 $\mu g/cm^2$/hr) to the $2^{nd}$ hydration phase (457.3 $\mu g/cm^2$/hr) suggests the disruption of skin barrier function due to extensive hydration. The permeation profile of NA with 3% pMPC in the donor solution showed monophasic pattern: the steady state flux (10.9 $\mu g/cm^2$/hr) without abrupt increase of the flux. The degree of NA permeation rate decreased in a concentration-dependent manner of pMPC. TEM of skin equilibrated with water or 2% pMPC for 12 h showed that corneocytes are still cohesive and epidermis is tightly bound to dermis in 2% pMPC-treated skin, while wider separation between corneocytes and focal dilations in inter-cellular spaces were observed in water-treated skin. This result suggests that pMPC could protect the barrier property of the stratum corneum by preventing the disruption of ILB structure caused by extensive skin hydration during skin permeation study.

• The Journal of Engineering Geology
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• v.33 no.1
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• pp.137-149
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• 2023

In order to intuitively grasp the earth pressure direction acting on the structure and displacement state, displacement data in the horizontal and vertical directions were processed using the sigmoid function. A displacement coordinate system was set up for each axis. The system can intuitively check the current displacement and assess the management stage of each point. A displacement path can be compiled from continuously recorded points, allowing trends in the displacement's history and stress direction to be known. Analysis of data measured for excavated ground, found that displacement occurred in the direction of destressing at all points, and that the points' management state steady. Similar behavior trends were found among measurement points with high spatial correlation, whereas differing behavior trends occurred among measurement points with low spatial correlation. If the correlation analysis of the precursor and behavior area is performed using the continuously distributed surface settlement data and displacement coordinate system, it will be possible to predict the failure time and area.

• 중국학논총
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• no.53
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• pp.1-16
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• 2017

The grammatical meaning of "了", a marker of aspects in Mandarin has been described differently among researchers, three of which are to express the end, a certain phase of event and the start of an event or a state. This paper consists of four parts. Set out below is a summary of main points of each part. The first chapter elaborates on the results of previous related studies, points out the purpose and meaning of the current study. Chapter two introduces marked "了" including concepts of the meaning and "Done", "complete whole", "realization" Corresponds to the case study. And explains syntactic and semantic features of the "了", highlights the look "of the了" problems of syntax and semantics, "了2" made three domains Limit 3 Including of the knowledge. This chapter holds that its grammartical meaning can be described as follows an event has taken place or a state has formed prior to a certain reference time, and that the grammartical meanings mentioned above can be considered sub - grammartical meanings of "了",which result from different prominence of the phases of an event. Chapter three explains tag component of the "了1", "了2". It discusses limited constraints, first place for "了" syntactic meaning, "The End" and "extension" of the opposition for "了" is looming around the root system, "了" and "着" sentence structure and semantic and syntactic forms. Chapter four discusses bounded features of the 了. It mainly focuses on "了"of bounded / unbounded function. It analyses the functions of components and explains a bounded function of "了1" and a bounded function of "了2". Chapter five is the conclusion. It consolidates and summarises the previous contents of the paper and points out the conclusion.

• Progress in Superconductivity
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• v.12 no.1
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• pp.51-56
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• 2010

$^{13}C$ NMR (nuclear magnetic resonance) measurements have been performed to investigate the local electronic structure of a superconducting graphite intercalation compound $CaC_6$ ($T_c$ = 11.4 K). A large number of single crystals were stacked and sealed in a quartz tube for naturally abundant $^{13}C$ NMR. The spectrum, Knight shift, linewidth, and spin-lattice relaxation time $T_1$ were measured in the normal state as a function of temperature down to 80 K at 8.0 T perpendicular to the c-axis. The $^{13}C$ NMR spectrum shows a single narrow peak with a very small Knight shift. The Knight shift and the linewidth of the $^{13}C$ NMR are temperature-independent around, respectively, +0.012% and 1.2 kHz. The spin-lattice relaxation rate, $1/T_1$, is proportional to temperature confirming a Korringa behavior as for non-magnetic metals. The Korringa product is measured to be $T_1T\;=\;210\;s{\cdot}K$. From this value, the Korringa ratio is deduced to be $\xi$ = 0.73, close to unity, which suggests that the independent-electron description works well for $CaC_6$, without complications arising from correlation and many-body effects.

• BMB Reports
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• v.40 no.4
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• pp.467-474
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• 2007

Autosomal recessive polycystic kidney disease (ARPKD) is one of the important genetic disorders in pediatric practice. Mutation of the polycystic kidney and hepatic disease gene 1 (PKHD1) was identified as the cause of ARPKD. The gene encodes a 67-exon transcript for a large protein of 4074 amino acids termed fibrocystin, but its function remains unknown. The neoplastic-like in cystic epithelial proliferation and the epidermal growth factor/epidermal growth factor receptor (EGF/EGFR) axis overactivity are known as the most important characteristics of ARPKD. Since the misregulation of $Ca^{2+}$ signaling may lead to aberrant structure and function of the collecting ducts in kidney of rat with ARPKD, present study aimed to investigate the further mechanisms of abnormal proliferation of cystic cells by inhibition of PKHD1 expression. For this, a stable PKHD1-silenced HEK-293T cell line was established. Then cell proliferation rates, intracellular $Ca^{2+}$ concentration and extracellular signal-regulated kinase 1/2 (ERK1/2) activity were assessed after treatment with EGF, a calcium channel blocker and agonist, verapamil and Bay K8644. It was found that PKHD1-silenced HEK-293T cell lines were hyperproliferative to EGF stimulation. Also PKHD1-silencing lowered the intracellular $Ca^{2+}$ and caused EGF-induced ERK1/2 overactivation in the cells. An increase of intracellular $Ca^{2+}$ in PKHD1-silenced cells repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation. Thus, inhibition of PKHD1 can cause EGF-induced excessive proliferation through decreasing intracellular $Ca^{2+}$ resulting in EGF-induced ERK1/2 activation. Our results suggest that the loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD by modulation of intracellular $Ca^{2+}$.

• Earthquakes and Structures
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• v.10 no.2
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• pp.367-388
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• 2016

Eccentrically braced frames (EBFs) represent an attractive lateral load resisting steel system to be used in areas of high seismicity. In order to assess the likely damage for a given intensity of ground shaking, fragility functions can be used to identify the probability of exceeding a certain damage limit-state, given a certain response of a structure. This paper focuses on developing a set of fragility functions for EBF structures, considering that damage can be directly linked to the interstorey drift demand at each storey. This is done by performing a Monte Carlo Simulation of an analytical expression for the drift capacity of an EBF, where each term of the expression relies on either experimental testing results or mechanics-based reasoning. The analysis provides a set of fragility functions that can be used for three damage limit-states: concrete slab repair, damage requiring heat straightening of the link and damage requiring link replacement. Depending on the level of detail known about the EBF structure, in terms of its link section size, link length and storey number within a structure, the resulting fragility function can be refined and its associated dispersion reduced. This is done by using an analytical expression to estimate the median value of interstorey drift, which can be used in conjunction with an informed assumption of dispersion, or alternatively by using a MATLAB based tool that calculates the median and dispersion for each damage limit-state for a given set of user specified inputs about the EBF. However, a set of general fragility functions is also provided to enable quick assessment of the seismic performance of EBF structures at a regional scale.

• Journal of Advanced Navigation Technology
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• v.8 no.2
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• pp.190-196
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• 2004

The development of a new hardware structure which can implement the viterbi algorithm efficiently is required for applications such as a software radio because the viterbi algorithm, which is an error correction code function for the second and the third generation of mobile communication, needs a lot of arithmetic operations. The length of K in the viterbi algorithm different from each standard, for examples, K=7 in case of IS-95 standard and GSM standard, and K=9 in case of WCDMA and CDMA2000. In this paper, we propose a new hardware structure of an adaptive viterbi decoder which can decode the constraint length in K=3~9 and the data rate in 1/2 ~ 1/3. Prototyping results targeted to Altera Cyclon EPIC20F400C8, shows that the proposed hardware structure needs maximum 19,276 logic elements and power dissipation of 222.6 mW.

• Journal of the Korea institute for structural maintenance and inspection
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• v.18 no.2
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• pp.64-73
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• 2014

This study proposes a load identification for the safety monitoring of the steel structure based on measured strain data. Instead of parameterizing the stiffness of structure in the existing system identification researches, the loads on a structure and a matrix (the unit strain matrix) defined by the relationship between strain and load on structure are parameterized in this study. The error function is defined by the difference between measured strain and strain estimated by parameters. In order to minimize this error function, the genetic algorithm which is one of the optimization algorithm is applied and the parameters are found. The loads on the structure can be identified through the founded parameters and measured strain data. When the loads are changed, the unmeasured strains are estimated based on founded parameters and measured strains on changed state of structure. To verify the load identification algorithm in this paper, the static experimental test for 3 dimensional steel frame structure was implemented and the loads were exactly identified through the measured strain data. In case of loading changes, the unmeasured strains which are monitoring targets on the structure were estimated in acceptable error range (0.17~3.13%). It is expected that the identification method in this study is applied to the safety monitoring of steel structures more practically.