• Title/Summary/Keyword: Staphylococcus sp.

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Characterization of a Coagulase-Negative Staphylococcus sp. Isolated from Raw-Milk having Reduced Susceptibility to Vancomycin (원유시료에서 분리된 반코마이신 저항성 Coagulase-Negative Staphylococcus 균주의 특성)

  • 하남주;최성숙
    • YAKHAK HOEJI
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    • v.45 no.5
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    • pp.491-493
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    • 2001
  • Coagulase-negative Staphylococcus sp #39, isolated from raw-milk showed reduced susceptibility to vancomycin. The minimun inhibitory concentration for strain #39 was at 8$\mu\textrm{g}$ of vancomycin per ml. Transmitting electron microscopy displayed that this strain had a 2.5-3.5 times thicker cell wall than a vancomucin sensitive strain of Staphylococcus sp. The strain #39 also had an increased cell volume. These data indicate that the reduced susceptibility may be due to the thickness of the cell wall of the test strain.

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Sphingobacterium sp. SW-09 Effectively Degrades Phenanthrene, a Polycyclic Aromatic Hydrocarbon, in a Soil Microcosm (Sphingobacterium sp. SW-09에 의한 토양환경에서의 다환 방향족탄화수소인 페난스렌의 분해)

  • Son, Seung-Woo;Chang, Hey-Won;Kim, Sung-Kuk;Chang, Jong-Soo
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1511-1517
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    • 2011
  • We isolated a potent phenanthrene-degrading bacterium from oil-contaminated soils of Suzhou, China, and assessed the potential use of these bacteria for bioremediation of soils contaminated by polycyclic aromatic hydrocarbons (PAHs) in a microcosm. Based on 16S rDNA sequencing, we identified this bacteria as Sphigobacterium sp. SW-09. By PCR amplification, we also identified catechol 2,3-dioxygenase genes (nahH genes) mediating PAH degradation. Staphylococcus sp. KW-07, which has been identified in our previous study, showed potential for use in bioremediation of oil-contaminated soils. In this experiment, we compared the rate of phenanthrene-degradation between Staphylococcus sp. KW-07 and Sphingobacterium sp. SW-09 in a microcosm condition. Newly isolated Sphingobacterium sp. SW-09 showed a higher phenanthrene-degradation rate than that of Staphylococcus sp. KW-07 in soil microcosms. Together, our results suggest that the Sphingobacterim sp. SW-09 strain isolated from the Suzhou area may also be useful in bioremediation of PAH-contaminated soils.

The Bacterial Contamination in Glasses for Vision Correction (시력 교정용 안경의 세균 오염)

  • Kim, Heung-Soo;Hwang, Seock-Yeon;Yun, Chi-Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.1
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    • pp.67-73
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    • 2013
  • Purpose: Recently, bacterial contamination of equipment and accessories required for vision correction has become a main causal factor in ophthalmic diseases. Thus, We investigated on both the actual condition of bacterial contamination from glasses of vision correction. Methods: Investigation of microorganisms was carried out with a group of 145 glasses wearers, composed of 36 elementary school students, 37 middle school students, 38 high school students, 10 college students, and 32 aged men. Results: Seventeen species of bacteria are detected from glasses of vision correction: B. cereus, B. licheniformis, Bacillus sp., CNS, Enterococcus sp., Escherichia coli, Proteus sp., Pseudomonas sp., Serretia sp., Streptococcus sp., Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus hemolyticus,, Acinetobacter sp., Enterobacter cloacae, GNR, and Pseudomonas aeruginosa. Among 17 species of bacteria, there are some potential causative agents for keratitis, corneal ulcer, Acute dacryocystitis, Orbital cellulitis, Periphlebitis retinae, Marginal blepharitis, and Acute conjunctivitis. Enterobacter cloacae, Pseudomonas aeruginosa and Staphylococcus epidermidis cause keratitis. Pseudomonas sp., and Staphylococcus aureus cause corneal ulcer. Staphylococcus aureus causes acute dacryocystitis, orbital cellulitis, periphlebitis retinae, marginal belpharitis. Streptococcus hemolyticus causes acute conjunctivitis. Conclusions: In summation, it is verified that hazardous, opportunistic and infectious microorganisms exist in glasses for vision correction. Ophthalmic diseases are predicted. Therefore, supplementary research on the development of a cleaning solution to cleanse the infection and of an effective method to remove microorganisms is required.

Ecological Studies on the Causative Agents of Food Poisoning from Food Animals - 1. Patterns on the Outbreaks and Antimicrobial Susceptibility of Causative Agents Isolated from Bovine Mastiffs in a Rural Area (축산유래 식중독 세균에 관한 생태학적 연구 제1보 : 일부 농촌지역 젖소 유방염의 발생양상 및 그 원인균이 항균요법제에 관한 감수성)

  • 정희곤
    • Journal of Environmental Health Sciences
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    • v.20 no.2
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    • pp.90-98
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    • 1994
  • Introduction : Bovine mastitis is an economically and a hygienically important disease of dairy cows. Many factors predispose to bovine mastitis and an understanding of these is essential for systems of effective mastiris control to be formulated. The presence of non-pathogenic bacteria on body surfaces can protect against invasion by more pathogenic organisms. Bacteria of low pathogenicity (minor pathogens) are frequently isolated from the healthy bovine udder and may play an important role in protecting the udder from infection with pathogenic bacteria. The treatment of bovine mastitis is important for choosing adequate antimicrobias, and it take the base on the result of susceptibility to antimicrobias. Therefore, the current of numbers feeding dairy cattle were increasing and prevalence rate of bovine mastiris was occurred in 1.5~57.3%.(abbreviation)

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Antibacterial Activities of Edible Plant Extracts against Strawberry Spoiling bacteria Staphylococcus sp. (식용식물의 유기용매 추출물로부터 딸기부패균 Staphylococcus sp.에 대한 항균활성 검정)

  • 하철규;이동규;강선철
    • KSBB Journal
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    • v.15 no.3
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    • pp.226-231
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    • 2000
  • Antibacterial activities of edible plant extracts were investigated to develop natural antimicrobial agents protecting horticultural products from spoiling-microorganisms during their storage. Crude extracts of Artemisa capillaris Allium tuberosum Ailanthus altissima Zanthoxylum pieperitum Pinus densiflora Morus alba lxeris dentata and Allium sativum showed remarkable antibacterial activities against Escherichia coli K 12 and Bacillus subtilis KCTC 1028 After solvent extraction of the crude extracts with n-hexane ethyl acetate chloroform and water in sequence each fractions was re-examined for the antbacterial activities. As results the ethyl acetate fractions of A. capillaris Aaltissima, P. densiflora and I. dentata and all fractions of Z. piperitum and A. sativium showed relatively strong antibacterial activities against E. coli and B. subtilis and the ethyl acetate fraction of A. altissima was the strongest(6mm and 7mm respectively) against two strawberry-spoiling bacteria isolated and identified at our laboratory as Staphylococcus sp. TG-101 and Staphylococcus sp. TG-102.

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Comparison of fatty acid composition of Staphylococcus sp isolated from bovine mastitis milk (유방염 감염 우유에서 분리된 Staphylococcus sp의 지방산 조성 비교)

  • Kim, Soon-Tae;Kim, Sin;Kim, Sang-Young;Son, Jae-Kweon
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.37-45
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    • 1997
  • The result of API staph-ident system was compared with cellular fatty acid composition for the identification of Staphylococcus species isolated from cattle. Isolated strains from cattle were correctly identified to S aureus, S intermedius, S hyicus, S simulans, S saprophyticus, S epidemis, S sciuri and S xylosus by API staph-ident system. The correlation between bacterial cellular fatty acid profile and Staphylococcus species isolated to API STAPH-IDENT system were. In conclusion, the result presented indicate that Staphylococci can be indentified to the species level by the cellular fatty acid profiles. Moreover, computerized fatty acid profile correlative anaylsis can be applied for determining identify of Staphylococcus species.

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Streptomyces sp. DG-2 with Anti-MRSA (Methicillin Resistant Staphylococcus aureus) Activity (항 MRSA (Methicillin Resistant Staphylococcus aureus) 활성을 나타내는 Streptomyces sp. DG-2)

  • Jeong, Seong-Yun
    • Journal of Marine Bioscience and Biotechnology
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    • v.9 no.2
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    • pp.49-57
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    • 2017
  • We isolated marine bacterium, isolate DG-2 which produces the antibiotics against MRSA (methicillin-resistant Staphylococcus aureus). This isolate DG-2 was examined by its morphological, biochemical properties, and 16S rRNA sequencing analysis. And then, isolate DG-2 was identified to the genus Streptomyces. Therefore, this isolate was designated as Streptomyces sp. DG-2. Streptomyces sp. DG-2 grew relatively well at $25^{\circ}C$, pH 7.0, and NaCl 1.0%. For the pre-purification of the bioactive compounds, DG-2 was fermented in 30 L PPES-II medium, and the culture filtrates of DG-2 was extracted by ethyl acetate. The ethyl acetate extract of DG-2 showed the significant anti-MRSA and antibacterial activities.

Population Density Changes of Bacteria Causing Soybean Sprout Rot on Soybean Pods (콩 꼬투리에서 서식하는 세균 및 콩나물 부패균의 밀도 변화)

  • 이은정;한광섭;심명용;최재을
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.41-45
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    • 1999
  • Bacterial population densities on soybean pods from Chungnam province ranges 105~106 CFU/$\textrm{cm}^2$, whereas those of bacteria causing sprout rot ranged 0~103 CFU/$\textrm{cm}^2$. Erwinia chrysanthemi, Xanthomonas campestris pv. glycines, Staphylococcus sp., and Micrococcus sp. were identified as pathogenic bacteria causing soybean sprout rot. The population density of X. campestris pv. glycines was higher than those of other bacteria.

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Population Density Changes of Bacteria and Soybean Sprout Rotting Bacteria on Soybean Leaves (콩 잎에 서식하는 세균 및 콩나물 부패균의 밀도 변화)

  • 최재을;이은정;신철우
    • Korean Journal of Plant Resources
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    • v.12 no.2
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    • pp.152-160
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    • 1999
  • Bacterial population density on soybean leaves was $10^2~10^5CFU/cm^2$. Bacterial population density was increased by progress of plant growth stage. Population density of soybean sprout rotting bacteria on soybean leaves was $0~10^3CFU/cm^2$. Population density of soybean sprouts rotting bacteria was related to cultivating area, but not related to plant growth stage. Cultivar and population density of soybean sprout rotting bacteria were less corelated, and varied by plant growth stages and plant parts. Erwina cypripedii, E. carotovora subsp. carotovora, Xanthomonas campestris pv. glycines, Staphylococcus sp., and Micrococcus sp. were identified as pathogenic bacteria causing soybean sprout rot. In generally population density of E. cypripedii, E. carotovora subsp. carotovora, Micrococcus sp., and X. campestris pv. glycines were high.

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Isolation and Characterization of Acid Protease Produced by Staphylococcus sp. CB2-3 from Digestive Organ of Harmonia axyridis (무당벌레 소화기관으로부터 산성 단백질 분해효소를 생산하는 Staphylococcus sp. CB2-3의 분리 및 특성)

  • Kim, Se-Jong;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.47 no.3
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    • pp.255-262
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    • 2011
  • Six protein-degrading bacteria were isolated from digestive organ of Harmonia axyridis. These isolates were categorized as Staphylococcus sciuri subsp. sciuri (3 strains), Bacillus subtilis (1 strain), and Bacillus thuringiensis (2 strains) by 16S rRNA gene sequence analysis. The Staphylococcus sp. CB2-3 was selected as a protease-producing bacterium which showed the highest protease activity of 58.5 U/ml at the pH 5.0 medium. The optimal pH and temperature of protease activity were pH 5.0 and $40^{\circ}C$, respectively. This acid protease had a relatively high stability of 80% between $30-50^{\circ}C$ at broad temperature range. The opimal medium compositions of carbon, nitrogen and mineral source for cell growth and protease activity were investigated. When sorbitol (0.5%) was used as carbon source, enzyme activity was increased about 2 times than that of the basal medium. When skim milk (0.5%) was used as nitrogen source, activity was increased about 2.5 times than that of the control. Cell growth and enzyme activity were increased by mineral source such as KCl, $K_2HPO_4$, $FeSO_4$, but was completely inhibited by divalent ions such as $Co^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Cu^{2+}$.