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http://dx.doi.org/10.5352/JLS.2011.21.11.1511

Sphingobacterium sp. SW-09 Effectively Degrades Phenanthrene, a Polycyclic Aromatic Hydrocarbon, in a Soil Microcosm  

Son, Seung-Woo (Suzhou Singapore International School)
Chang, Hey-Won (Dongducheon Forgein Language High School)
Kim, Sung-Kuk (Department of Life Science, College of Natural Science, Daejin University)
Chang, Jong-Soo (Department of Life Science, College of Natural Science, Daejin University)
Publication Information
Journal of Life Science / v.21, no.11, 2011 , pp. 1511-1517 More about this Journal
Abstract
We isolated a potent phenanthrene-degrading bacterium from oil-contaminated soils of Suzhou, China, and assessed the potential use of these bacteria for bioremediation of soils contaminated by polycyclic aromatic hydrocarbons (PAHs) in a microcosm. Based on 16S rDNA sequencing, we identified this bacteria as Sphigobacterium sp. SW-09. By PCR amplification, we also identified catechol 2,3-dioxygenase genes (nahH genes) mediating PAH degradation. Staphylococcus sp. KW-07, which has been identified in our previous study, showed potential for use in bioremediation of oil-contaminated soils. In this experiment, we compared the rate of phenanthrene-degradation between Staphylococcus sp. KW-07 and Sphingobacterium sp. SW-09 in a microcosm condition. Newly isolated Sphingobacterium sp. SW-09 showed a higher phenanthrene-degradation rate than that of Staphylococcus sp. KW-07 in soil microcosms. Together, our results suggest that the Sphingobacterim sp. SW-09 strain isolated from the Suzhou area may also be useful in bioremediation of PAH-contaminated soils.
Keywords
Polycyclic aromatic hydrocarbon (PAH); bioremediation; phenanthrene; Sphingobacterum sp. SW-09; Staphylococcus sp. KW-07;
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