KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antibacterial Activities of Edible Plant Extracts against Strawberry Spoiling bacteria Staphylococcus sp.

식용식물의 유기용매 추출물로부터 딸기부패균 Staphylococcus sp.에 대한 항균활성 검정

  • Published : 2000.06.01
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Abstract

Antibacterial activities of edible plant extracts were investigated to develop natural antimicrobial agents protecting horticultural products from spoiling-microorganisms during their storage. Crude extracts of Artemisa capillaris Allium tuberosum Ailanthus altissima Zanthoxylum pieperitum Pinus densiflora Morus alba lxeris dentata and Allium sativum showed remarkable antibacterial activities against Escherichia coli K 12 and Bacillus subtilis KCTC 1028 After solvent extraction of the crude extracts with n-hexane ethyl acetate chloroform and water in sequence each fractions was re-examined for the antbacterial activities. As results the ethyl acetate fractions of A. capillaris Aaltissima, P. densiflora and I. dentata and all fractions of Z. piperitum and A. sativium showed relatively strong antibacterial activities against E. coli and B. subtilis and the ethyl acetate fraction of A. altissima was the strongest(6mm and 7mm respectively) against two strawberry-spoiling bacteria isolated and identified at our laboratory as Staphylococcus sp. TG-101 and Staphylococcus sp. TG-102.

원예산물의 저온저장중 부패방지를 위한 천연항균제 개발의 일환으로 식용 가능한 식물을 채집하여 E. coli K 12, B. subtilis K KCTC 1028 및 딸기부패균에 대한 항균활성을 조사하였다. 사철 쑥, 가죽나무 잎, 초피 잎, 적송 잎, 뽕 잎, 씀바귀, 마늘의 즙액은 E. coli와 B. subtilis에 대하여 높은 항균활성을 보였다. 이들 식물체 즙액으로부터 n-hexane, ethyl ace떠te, chloroform 및 물로 용매분획한 분획물로 항균성을 재검색한 결과, 사철쑥, 가죽나무 잎, 적송 잎, 씀바귀의 ethyl acetate 분획과 초피 잎과 마늘의 모 든 분획에서 비교적 높은 향균활성을 보였으며 가죽나무 잎의 ethyl acetate 분획 이 가장 높은 항균활성(6 nun)을 보였다. 한편 딸기의 저온저장중인 과육으로부터 두 종의 부패 세균을 분리하 여 각각 Staphylococcus sp. TG-IOI과 Staphylococcus sp. TG-I02 로 동정하였다. 이들 균주에 대한 항균성을 조사한 결과, 가죽나 무 잎의 ethyl acetate 분획에서 두 균주에 대하여 가장 높은 저 해효과(각각 6 mm, 7 nun)를 보였다.

Keywords

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