• Title/Summary/Keyword: Squeezing

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Determination of Ceramide-Induced Apoptotic Cell Death in Mouse Granulosa Cell Cultured In Vitro (생쥐 난소 과립세포의 체외배양중 세라마이드에 의한 자연세포사의 측정)

  • Kim, Jong-Hoon;Kim, Gyoung-Mi;Yoon, Yong-Dal
    • Development and Reproduction
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    • v.2 no.1
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    • pp.1-8
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    • 1998
  • In mammalian ovary, major portion(>99%) of ovarian follicles undergo atresia. Recent studies have shown that this phenomenon is mediated via GC apoptosis. Ceramide, a product of sphingomyelin hydrolysis, has been proposed as a novel lipid second messenger with specific roles in mediating antiproliferative responses including apoptosis and cell cycle arrest. In the present study, we have examined the effect of ceramide on apoptotic cell death of GC in vitro. GCs were harvested by squeezing the antral follicles from the immature mice (3-4 weeks) and cultured in MEM medium with 10% fetal bovine serum. The cells were treated with various concentrations of ceramide (0 to 50 \mu M)and cultured up to 24 h.Cell death was determined by MTT cell viability assay and apoptosis was examined by acridine orange staining, in situ 3'-end labeling(TUNEL), and flow cytometry. Ceramid treatment induced apoptotic cell death of GC in a time- and a dose-dependent manner. Results of flow cytometric analysis showed that creamide-induced cell death was mostly confined to the $G_{0}$/$G_{1}$ cells. these results provide an evidence for ceramide as a lipid second messenger of apoptosis in mouse GC.

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Antioxidant capacity and nutritive components from biji sub-micron suspension by ultra-high pressure homogenization process (초고압균질 가공에 의해 제조된 초미세 비지 현탁액의 영양성분 및 항산화성)

  • Lee, Seon-Ho
    • Food Science and Preservation
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    • v.22 no.5
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    • pp.714-720
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    • 2015
  • Biji is a tofu by-product made by squeezing the liquid out of the dregs left over from making tofu. The objectives of this research were to break the biji into sub-micron-scale particles and to prepare a biji sub-micron suspension having increased quality characteristics such as antioxidant capacity, polyphenol, dietary fiber, and soluble protein contents, in comparison with untreated biji. Disruption of biji by an ultra-high-pressure homogenization (UHPH) process was used to prepare the sub-micron suspension of biji. UHPH can be used to prepare emulsions or suspensions with extremely small particle sizes. The effect of the UHPH process on biji was studied at 1,000, 1,500, and 2,000 bar. The extraction yield increased up to 1.64 times by breaking the biji to form a sub-micron suspension. The soluble dietary fiber, protein, and free amino acid contents were increased by the UHPH process (p<0.05), in comparison with those of untreated biji. Furthermore, the total sugar, free sugar, and total polyphenol contents of biji were augmented by the UHPH process. Antioxidant activity (ABTS) after exposure to UHPH (1,000-2,000 bar) was well retained (p<0.05). The extraction efficiencies and nutritive components were enhanced by increasing the pressure in the UHPH process.

A Case of Thigh Stump Pain with Unidentified Complaints (다양한 증상을 호소한 대퇴 절단지통 1예)

  • Cha, Young-Deog;Kim, Il-Ho;Kim, Yu-Jae;Kim, Chun-Sook;Ahn, Ki-Rhang;Park, Wook;Kim, Sung-Yell
    • The Korean Journal of Pain
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    • v.7 no.1
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    • pp.100-105
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    • 1994
  • This is a study of decrease in both stump pain and unidentified complaints after removal of neuroma on an amputated left thigh. The patient was a 44 year old woman who received an operation after a motorcycle accident 20th of March, 1991. She started a rehabilitation program in early June of the same year. How ever the patient complained of a squeezing pain on the amputated area. This symptom became more severe after the removal of the nails in September. The pain was perceived as a mental problem and the patient was released from the previous hospital. The pain continued and on the 9th of March, 1992, the patient was introduced to our pain clinic. The patient complained about the cold sensation and pressure pain of the amputated area at the beginning. Later she also expressed various unidentified complaints. No improvement resulted after conducting an epidural block and a lumbar sympathetic ganglion block. MMPI test showed psychological instability. Local injection showed some positive effects, which led to considerations concerning the possibility of neuroma. After confirming the existence of neuroma through CT and MRI, neuromectomy was performed. After the removal of neuroma, the unidentified complaints as well as the stump pain decreased.

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A Study on Development of Wrinkle Evaluation Software and Verification of Skin Wrinkle Improvement of Cog Suture (주름 평가 소프트웨어 개발과 Cog형 봉합사의 피부 주름 개선 검증에 관한 연구)

  • Jeong, Jin-Hyoung;Lee, Sang-Sik
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.12 no.4
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    • pp.336-342
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    • 2019
  • With the entry of an aging society, the average life span of accreditation has been extended. Therefore, interest in the appearance of men and women in modern society has increased. It is the wrinkles of the face that can judge the most outwardly. People tend to have various kinds of treatments to have a clean, wrinkle-free and resilient healthy skin regardless of sex. There is a lot of practice of lifting procedures in one of the procedures. A suture using a melting thread is a method of lifting by squeezing it into the skin as a non-incision type centering on a region where the thread can be fixed in the skin by injecting it into the subcutaneous fat layer. To evaluate the lifting efficacy of Cog - type suture for the improvement of skin wrinkles, preclinical experiments were conducted. We developed a wrinkle evaluation program using Labview. Data from preclinical experiments were used at 8 weeks after suturing. The average wrinkle depth was 415.6 mm in the control group. At 8 weeks, the depth of wrinkles was deepened to 888.3mm due to the aging process of the control group. On the other hand, the depth of the wrinkles before surgery was 640.3 mm in the suture group. It was confirmed that the depth of wrinkles decreased to 566.5mm at 8th week after the suture operation.

The politics of shadow education market expansion in Korea: Focused on mobilization capabilities and strategies of suppliers (한국 사교육 정책의 작동 메커니즘에 대한 정치적 분석: 공급자의 동원능력과 시장전략을 중심으로)

  • Hwang, Gyu-Seong
    • 한국사회정책
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    • v.20 no.2
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    • pp.233-260
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    • 2013
  • Despite various policies have been implemented to curb shadow education in Korea, it has continued to grow in recent two decades. This study investigates the expansion mechanism of shadow education focused on mobilization capabilities and market strategies of the suppliers. The success and failure of policy toward shadow education depends on how effectively it could block off the way by which the suppliers as the most important actors in politics of shadow education market mobilize consumers' anxieties. But shadow education policies have failed in two points. First, they have lacked honest intention to stop its proliferation. The Constitutional Court Decision Against Anti-Out-Of-School Classes Legislation of 2000 widened the windows of opportunity for the suppliers, and 5.31 educational reform of 1995 was neutral to their mobilization capabilities, though seemingly designed to control shadow education. This policy orientation, which reflected neoliberal Gesinnungsethik defective of Verantwortungsethik, stimulated shadow education to expand in that suppliers' mobilization capabilities were reinforced or remained intact. Second, shadow education suppliers have succeeded in mobilizing the desire and anxiety of potential consumers. To cope with government's policy including improving the qualities of public education, realignment of college entrance systems, and meeting the shadow education needs, they have developed various market strategies such as management of existing demands, creation of responsive demands, and squeezing out new demands. They have succeeded in nullifying policies by employing or mixing strategies with effect. Policy decisions in the future need to be made with reference to Verantwortungsethik, and be more cautious to socio-political contexts of Korea, to mobilization capabilities and market strategies of the suppliers in particular.

Folding Analysis of Paper Structure and Estimation of Optimal Collision Conditions for Reversal (종이구조물의 접기해석과 반전을 위한 최적충돌조건의 산정)

  • Gye-Hee Lee
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.36 no.4
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    • pp.213-220
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    • 2023
  • This paper presents a model simulating the folding process and collision dynamics of "ddakji", a traditional Korean game played using paper tiles (which are also referred to as ddakji). The model uses two A4 sheets as the base materials for ddakji. The folding process involves a series of boundary conditions that transform the wing part of the paper structure into a twisted configuration. A rigid plate boundary condition is also adopted for squeezing, establishing the shape and stress state of the game-ready ddakji through dynamic relaxation analysis. The gaming process analysis involves a forced displacement of the striking ddakji to a predetermined collision position. Collision analysis then follows at a given speed, with the objective of overturning the struck ddakji--a winning condition. A genetic algorithm-based optimization analysis identifies the optimal collision conditions that result in the overturning of the struck ddakji. For efficiency, the collision analysis is divided into two stages, with the second stage carried out only if the first stage predicts a possible overturn. The fitness function for the genetic algorithm during the first stage is the direction cosine of the struck ddakji, whereas in the second stage, it is the inverse of the speed, thus targeting the lowest overall collision speed. Consequently, this analysis provides optimal collision conditions for various compression thicknesses.

A study for Beating Filter Press Dewatering Technology (열(熱) 필터프레스 기술(技術)을 통한 슬러지 탈수율(脫水率) 향상(向上)을 위한 연구(硏究))

  • Lee, Jung-Eun;Kim, Dong-Su
    • Resources Recycling
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    • v.15 no.3 s.71
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    • pp.38-45
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    • 2006
  • The thermal filter press dewatering(TFPD) technology to improve the dewaterability through increasing the inner vapor pressure, lowering the filtration viscosity and forming the porosity easily within cake as applying the heat at the sludge layer was developed in this study. The hot water with temperature of $95^{\circ}C$ and pressure of $1.2kg_f/cm^2$ was supplied to the heating plate equipped between filter plates with plate size of $470{\times}470mm$ and material of polypropylene. Sludge was dewaterd by supplying pressure of $5kg_f/cm^2$ and then by squeezing pressure of $15kg_f/cm^2$. As a results of estimating the characteristics of thermal dewatering to consider the initial water content and organic content to be influenced by a period of water shortage and rainwater, the dewatered cake water content was about 35 wt% and dewatering velocity was $4DSkg/m^2{\cdot}hr$ under the rainwater period, and the dewatered cake water content was about 50 wt% and dewatering velocity was $1.5DSkg/m^2{\cdot}hr$ in the case of sludge of water shortage season. These results was superior to the mechanical dewatering performance with water content of 70wt% and dewatering velocity of $0.9DSkg/m^2{\cdot}hr$. On the base of the results of TFPD, energy consumpted to deal with DS(Dry Solid) of 1kg was estimated by 300 kJ. It was analyzed that the energy consumption of TFPD was decreased about one third with comparison to the dryer system. Dewatering velocity of this technology was faster than the one of mechanical dewatering equipment and it was easier to product low water content cake. Therefore, this technology was recognized that dewaterability was predominant because of the fast of dewatering velocity and production of low water content cake, and also this known as economical efficiency was excellent because of low energy consumption in comparison with dryer.

The Liver Protecting Effect of Pomegranate (Punica granatum) Seed Oil in Mice Treated with $CCl_4$ (사염화탄소를 처리한 생쥐에서 석류종자기름의 간보호 효과)

  • Kim, Dong-Heui;Deung, Young-Kun;Lee, Young-Mi;Yoon, Yang-Suk;Kwon, Ki-Rok;Park, Dae-Bok;Park, Seung-Kyu;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.36 no.3
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    • pp.173-182
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    • 2006
  • Recently, the pomegranate seed oil (PSO) has been reported to have various efforts including anti-cancer effect. In this study, we examined the liver-protecting effect of the PSO on the hepatotoxicity induced by $CCl_4$ using the BALB/c mice. The PSO was made from dried seeds of black pomegranate (Punica grantum) by heating and squeezing. The expreimental animals were divided into 3 groups; control group treated with olive oil only, experimental group 1 treated with $CCl_4$ only, and experimental group 2 treated with PSO and $CCl_4$. 24 hours after injection of $CCl_4$ into the peritoneal cavity, we collected the blood samples to measure the level of serological factors; aspartate aminotransferase(AST), alanine aminotransferase (ALT), total protein, albumin, total bilirubin, direct bilirubin and alkaline phosphatase. Simultaneously we observed the histological change of liver under the light and electron microscope. As the result, AST and ALT showed $88.7{\pm}14.9IU/L\;and\;22.0{\pm}3.12IU/L$ in the control group, $1963.7{\pm}1212.9IU/L\;and\;4495.4{\pm}2803.6IU/L$ in the experimental group 1, and $432.2{\pm}260.1IU/L\;and\;692.3{\pm}433.1IU/L$ in the experimental group 2. The experimental group 2 showed significant difference as compared with experimental group 1 (P<0.005). In histological study, the experimental group 2 was recovered than experimental group 1 which had abnormal mitochondria, increase of lysosomes, and severe necrosis at the central vein zones. These results indicated that the PSO had the liver protecting effect. However, The further study on the relationship between ingredients of pomegranate seed and liver protecting effect is in need.

The Production of Sex Determined Cattle by Embryonic Sexing Using Fluorescence In Situ Hybridization Technique (FISH 기법을 이용한 소 수정란의 성감별과 산자 생산)

  • Sohn, S.H.;Park, H.
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2007.05a
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    • pp.39-50
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    • 2007
  • Sexing from bovine embryos fertilized in vitro implicates a possibility of the sex controlled cattle production. This study was carried out to produce the sex determined cattle through the embryonic sexing by fluorescence in situ hybridization (FISH) technique. FISH was achieved in in vitro fertilized bovine embryos using a bovine Y-specific DNA probe constructed from the btDYZ-1 sequence. Using this probe, a male-specific signal was detected on 100% of Y-chromosome bearing metaphase specimens. The analyzable rate of embryonic sexing by FISH technique was about 93% (365/393) regardless of embryonic stages. As tested single blastomere by FISH and then karyotype with their biopsied embryos, the accuracy of sex determination with FISH was 97.6%. We tried the embryo transfer with sex determined embryos on 15 cattle. Among them, the 5 cattle delivered calf with expected sex last year.

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A Study on the Blood-Letting Therapy in Elementary Questions (("황제내경소문(黃帝內經素問)" 중(中) 사혈(瀉血)에 관한 연구(硏究))

  • Lee, Jun-Geun
    • Journal of the Korean Institute of Oriental Medical Informatics
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    • v.14 no.1
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    • pp.19-42
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    • 2008
  • Blood-Letting Therapy is a rational and ecological medical treatment by which we can heal most of the diseases by removing the static blood which precipitates in the blood vessel and blocks the flowing of blood. And the static blood is the generic term for the injurious, bad, dead and precipitated blood which is blocked the capillary vessel. The Yellow Emperor's Canon of Internal Medicine says that "the patient is treated with drugs internally and stone acupuncture externally. "In the old texts, the blood-letting therapy is mentioned as blood-letting, network vessel pricking, bloodletting, pricking, and arousing pulses etc and it is noted down as the method of network vessel pricking in 'On the Application of Needles' of Spiritual Pivot. Nine-pricking therapy, twelve-pricking therapy and five-pricking therapy are recorded in the methods of network vessel pricking and among them, the method of squeezing blood after pricking the affected part is explained as the network vessel pricking. There are four methods of network vessel pricking, pricking, picking, cluster needling and scatter-pricking and they are fluidly applied to the various symptoms of diseases. In 'On Discriminative Treating for Patients of Different Regions' in Elementary Questions, Ki-baek emphasizes "most of the local people, there are black in skin and loose in striate, and their diseases are mostly of carbuncle kind. It is suitable to treat the disease with stone therapy to prick with stone, so the stone therapy is transmitted from the east. "And in 'On the Corresponding Relation Between the Eum and Yang of Man and All Things' in Elementary Questions, when the Emperor asked Ki-Back, he answered "sthenia means the sthenia of evil, and deficiency means the deficiency of healthy energy. When the blood is sthenic, the evil should be discharged by pricking when out letting the blood; deficiency of vital energy is the asthenia of channels and network vessels, so the energy should drain from the channel which is not deficient, to replenish. "And in this case we can use the methods of 'Breaking out the static bloods', 'driving out the static bloods', blood-letting'. With this we can infer that the blood-letting therapy is made use of the important medical treatments from the ancient times. Especially in referring to the principles of treatment in The Yellow Emperor's Canon of Internal Medicine, it mostly alluded to acupuncture therapies and only eleven times to medicinal treatments. This is to verify that the blood-letting therapy formed the foundation of the medical art. In Dong's Therapy of Acupuncture-Moxibustion and Bloodletting, Dong Kyeong-Chang gave emphasis on the points that there must be extravasated bloods without exception in the serious illnesses which is old, unnatural, accompanying acute pains and so we can revive our body‘s sprit by circulating 'gi' and static blood piled up in the network vessel, regulating the weakness and strength, and controling the disharmony of the internal organs. The blood-letting therapy has effect on the orifice in emergency, such as fore draining, freeing network vessels, harmonizing gi and blood, relieving pain, dispersing swelling and concretion, sedation, resolving toxin as well as strengthening the heart, relieving itching. So it has distinguished effect on all kinds of medical treatment to the modern people. But by the change of social customs and the confucianism of confucius - it is widely spread on the period of North and South Dynasties, 'Wi' and 'Jin' in china and the period of the Three States in korea - The blood-letting therapy which was regarded as the most important medicinal treatment withered rapidly. And Confucius accentuated the importance of our body and all its members, loyalty and filial piety and banned any damage of our body under no circumstances. As a result of it, the therapy of blood-letting had a rapid decrease and barely kept itself in existence in both countries. What is worse, at the period of Japanese colonial rule of korea and our nation's founding of early stage, it has been withered by the high-handed policy to change Oriental Medicine into modern medical science. So the therapy of blood-letting barely kept itself in existence in some Buddhist temples. Another case, it has handed down as a old-fashioned quick fix in folk remedies. But all kinds of the contamination of heavy metals and the misuses of antibiotics are widely spread nowadays, which increased diseases of adult people and incurable diseases as modern society unavoidably made its way into a highly industrial society. To make us more miserable, the western medical science - the antibiotics and surgical operation medical science - already reveals itself into a limit. The necessity of a new medical science which can give a security to the patients who are suffering from the diseases of adult people and the incurable diseases is especially come into the force nowadays. In view of the results after bibliographically studying on the blood-letting Therapy in Elementary Questions of the Yellow Emperor's Canon of Internal Medicine, the blood-letting therapy has acted for the important Oriental medicinal science and has been clarified the prominent effects on the diseases of adult people and the incurable diseases. So it is regarded as an appropriate thing that we lay out a determined theory of the blood-letting therapy and of course prevent the unwanted side effects from inappropriate medicinal treatments, and make full use of clinic by elevating the curative value and that we win back our self-respect of medical treatment which is dominated from the western medical science and ultimately contribute to national medical welfare.

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