한국수정란이식학회:학술대회논문집 (Proceedings of the Korean Society of Embryo Transfer Conference)
- 한국수정란이식학회 2007년도 춘계학술세미나 및 워크숍
- /
- Pages.39-50
- /
- 2007
FISH 기법을 이용한 소 수정란의 성감별과 산자 생산
The Production of Sex Determined Cattle by Embryonic Sexing Using Fluorescence In Situ Hybridization Technique
-
Sohn, S.H.
(Department of Animal Science & Biotechnology, Jinju National University) ;
-
Park, H.
(Department of Animal Science & Biotechnology, Jinju National University)
- 발행 : 2007.05.18
초록
본 연구에서는 소의 체외 수정란에 대해 Y 염색체 특이 DNA 표지를 이용하여 FISH 기법으로서 수정란의 성감별을 수행하고 이를 이식하여 산전 성감별 개체 생산을 시도하였다. 본 연구에 이용된 Y-염색체 특이 DNA probe는 bovine male specific DNA로 알려진 btDYZ-1의 385bp 단편으로 제작하였고, Dig-PCR 방법으로 labeling하였다. 수정란의 성감별은 IVF로서 생산된
Sexing from bovine embryos fertilized in vitro implicates a possibility of the sex controlled cattle production. This study was carried out to produce the sex determined cattle through the embryonic sexing by fluorescence in situ hybridization (FISH) technique. FISH was achieved in in vitro fertilized bovine embryos using a bovine Y-specific DNA probe constructed from the btDYZ-1 sequence. Using this probe, a male-specific signal was detected on 100% of Y-chromosome bearing metaphase specimens. The analyzable rate of embryonic sexing by FISH technique was about 93% (365/393) regardless of embryonic stages. As tested single blastomere by FISH and then karyotype with their biopsied embryos, the accuracy of sex determination with FISH was 97.6%. We tried the embryo transfer with sex determined embryos on 15 cattle. Among them, the 5 cattle delivered calf with expected sex last year.