• The Korean Journal of Mycology
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• v.41 no.1
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• pp.52-55
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• 2013

Phoma glomerata (Corda) Wollenw. & Hochapfel is a pathogenic fungus causing spot diseases of plant leaves and fruits. This fungus is important in plant quarantine of seedlings and fruits in Korea. The aim of this study was to develop a sensitive and effective diagnostic method for P. glomerata detection in imported plants. The fungal species-specific PCR primers were designed based on the nucleotide sequences of the translation elongation factor 1 alpha gene and their specificity and sensitivity were tested. The designed primers named as PhoGlo-F and PhoGlo-R amplified specifically a 170 bp sized DNA band of the target gene from the genomic DNA of P. glomerata. No amplicon was produced from genomic DNAs of 16 other Phoma spp. and reference fungal species tested. Moreover, PhoGlo-F/PhoGlo-R primers successfully worked with real-time PCR technique. The detection limit of DNA content by conventional and real-time PCR were 10 pg and 1pg of the genomic DNA of P. glomerata, respectively. We believed that the developed makers would be very useful for P. glomerata detection.

• Research in Plant Disease
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• v.16 no.2
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• pp.125-128
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• 2010

Bacterial black spot disease of prune fruit (Prunus salicina cv. formosa) has outbroke around major prune production area, Gimcheon, Euiseong and Gunwi in Gyungbuk province and has caused severe economic loss. Integrons PCR primer was designed along with sample pre-incubation and nested PCR method to enhance detection sensitivity for early detection of bacteria in fields. Designed integrons PCR primer successfully detected Xanthomonas arboricola pv. pruni from field collected samples, fruit, leaf, branch and even in raindrop collected from prune orchard. Pre-incubation along with nested PCR enhanced sensitivity to detect X. arboricola pv. pruni from seemingly healthy looking, symptomless branches. Designed integrons PCR can be used in prune nursery fields and in plant quarantine practice for the detection of X. arboricola pv. pruni.

• Nuclear Engineering and Technology
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• v.39 no.5
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• pp.637-646
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• 2007

An UET (ultrasound excited thermography) has been used for several years for a remote non-destructive testing in the automotive and aircraft industry. It provides a thermo sonic image for a defect detection. A thermograhy is based On a propagation and a reflection of a thermal wave, which is launched from the surface into the inspected sample by an absorption of a modulated radiation. For an energy deposition to a sample, the UET uses an ultrasound excited vibration energy as an internal heat source. In this paper the applicability of the UET for a realtime defect detection is described. Measurements were performed on two kinds of pipes made from a copper and a CFRP material. In the interior of the CFRP pipe (70mm diameter), a groove (width - 6mm, depth - 2.7mm, and length - 70mm) was engraved by a milling. In the case of the copper pipe, a defect was made with a groove (width - 2mm, depth - 1mm, and length - 110 mm) by the same method. An ultrasonic vibration energy of a pulsed type is injected into the exterior side of the pipe. A hot spot, which is a small area around the defect was considerably heated up when compared to the other intact areas, was observed. A test On a damaged copper pipe produced a thermo sonic image, which was an excellent image contrast when compared to a CFRP pipe. Test on a CFRP pipe with a subsurface defect revealed a thermo sonic image at the groove position which was a relatively weak contrast.

• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2725-2730
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• 2013

Single C-reactive protein (CRP) molecules, which are non-specific acute phase markers and products of the innate immune system, were quantitatively detected on a gold-nanopatterned biochip using evanescent field-enhanced fluorescence imaging. The $4{\times}5$ gold-nanopatterned biochip (spot diameter of 500 nm) was fabricated by electron beam nanolithography. Unlabeled CRP molecules in human serum were identified with single-molecule sandwich immunoassay by detecting secondary fluorescence generated by total internal reflection fluorescence (TIRF) microscopy. With decreased standard CRP concentrations, relative fluorescence intensities reduced in the range of 33.3 zM-800 pM. To enhance fluorescence intensities in TIRF images, the distance between biochip surface and CRP molecules was optimally adjusted by considering the quenching effect of gold and the evanescent field intensity. As a result, TIRF only detected one single-CRP molecule on the biochip the first time.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.7
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• pp.1280-1286
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• 2001

In this Study, we try to suggest a system that recognize worker's regular motion more effectively First, based on frame difference that separates still background from movable object to video that make a film of worker's motion. The next, with edge detection, estimating the center of motion could recognize continuous motion. By action cognition system that design in this research films worker's action using fixed CCTV to supplement problem of action awareness system that is applied in existent industry spot, various mountings to get action information minimized. Also, shorten session that need in awareness enforcing action awareness through image subtraction and edge detection between frame to reduce time necessary to draw worker's body part special quality, expense designed inexpensive action cognition system as being efficient.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.16 no.3
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• pp.123-128
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• 2016

In manufacturing processing of a secondary battery, the visual inspection system is studied and developed to check the surface defects of the electrode plates. It consists of two parts, one is the hardware control and the other software implementation. The former is made up to the system configuration and the design of the optical system, the illuminations and the controllers. The latter is the detection algorithms of the surface defects. This system achieves the quality improvement of the electrode process and the price competitiveness. By using the proposed defects detection algorithms this system demonstrates the high reliability of spot, line, manhole, extraneous substance, scratch, and crater defect of a electrode plate surface.

• The Plant Pathology Journal
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• v.34 no.4
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• pp.327-334
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• 2018

Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease management. Based on the polyketide synthase gene (PKS) of Cochliobolus carbonum and the nonribosomal peptide synthetase gene (NRPS) of Cochliobolus heterostrophus, primer pairs were designed for PCR to simultaneously detect the two fungal pathogens and were specific and sensitive enough to be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds.

• 전자공학회논문지 IE
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• v.48 no.1
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• pp.16-24
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• 2011

In this paper, we developed a auto sorting system for T type welding nut using a vision inspector. We used edge and thread detection with histogram of image which is captured by machine vision camera. We also used a binary morphology operation for a detection of spot. As a result we performed numeric inspection of 0.1mm accuracy. This is impossible in old sorting system and inspector with naked eye. Also, we reduced the manufacturing unit cost to 25% and improved a production efficiency to 330%.

• Annual Conference of KIPS
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• 2013.11a
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• pp.1709-1712
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• 2013

교통 신호등은 운전자 혹은 보행자들의 뚜렷한 시인성 확보를 위해 가능한 주위 배경과 구분되는 색상, 모양, 질감 등으로 구성하여 설치되어 있는 특징을 가지고 있다. 결국 기존 교통 신호등 검출 연구들에서는 대부분 교통 신호등의 색상과 모양을 기반으로 한 검출 연구가 주류를 이루고 있는 것이 사실이다. 하지만, 외부 날씨, 복잡한 시내, 다른 물체와의 겹침 등의 문제로 인해 색상 및 모양 기반의 교통 신호등, motion blur, 검출 오류가 증가 되고 있다. 따라서 본 연구에서는 입력 영상에서 색상정보를 배제하고 motion blur나 밝기 변화에 덜 민감하고 먼 거리에서도 뛰어난 시인성을 가진 spot light 검출을 통해 입력 영상에서 가장 밝은 교통표지판 후보 영역들을 검출한다. 그리고 교통 신호등의 특징인 가능한 원형을 유지하고 있으며 원형 외부 색상과 내부 색상이 현저하게 두드러지는 영역을 maximally stable extremal regions (MSER) 알고리즘을 사용하여 입력 영상에서 후보 영역을 선택한다. 마지막으로, 검출된 영역들에서 교통 신호등 영역을 검출하기 위해 템플릿 매칭 방법을 적용한다. 제안한 방법을 도로 상에서 실험한 결과, 평균 94% 이상의 검출율을 제시하였고, 특히 야간 시간대에 검출율이 비교적 높게 제시되었다.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.9
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• pp.113-127
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• 2012

Face detection methods using image processing have been proposed in many different ways. Generally, the most widely used method for face detection is an Adaboost that is proposed by Viola and Jones. This method uses Haar-like feature for image learning, and the detection performance depends on the learned images. It is well performed to detect face images within a certain distance range, but if the image is far away from the camera, face images become so small that may not detect them with the pre-learned Haar-like feature of the face image. In this paper, we propose the far distance face detection method that combine the Aadaboost of Viola-Jones with a saliency map and user's attention information. Saliency Map is used to select the candidate face images in the input image, face images are finally detected among the candidated regions using the Adaboost with Haar-like feature learned in advance. And the user's eye-tracking information is used to select the interest regions. When a subject is so far away from the camera that it is difficult to detect the face image, we expand the small eye gaze spot region using linear interpolation method and reuse that as input image and can increase the face image detection performance. We confirmed the proposed model has better results than the conventional Adaboost in terms of face image detection performance and computational time.