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http://dx.doi.org/10.5423/RPD.2010.16.2.125

PCR Primer Developed for Diagnosis of Xanthomonas arboricola pv. pruni in Prune  

Ryu, Young-Hyun (Institute for Natural Products Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Lee, Joong-Hwan (Dept. of Plant Environment Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Kwon, Tae-Young (Dept. of Plant Environment Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Kim, Seung-Han (Bonghwa Alpine Medicinal Plant Experiment Station, Gyeongsangbuk-do Agricultural Research & Extension Services)
Kim, Dong-Geun (Institute for Natural Products Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Publication Information
Research in Plant Disease / v.16, no.2, 2010 , pp. 125-128 More about this Journal
Abstract
Bacterial black spot disease of prune fruit (Prunus salicina cv. formosa) has outbroke around major prune production area, Gimcheon, Euiseong and Gunwi in Gyungbuk province and has caused severe economic loss. Integrons PCR primer was designed along with sample pre-incubation and nested PCR method to enhance detection sensitivity for early detection of bacteria in fields. Designed integrons PCR primer successfully detected Xanthomonas arboricola pv. pruni from field collected samples, fruit, leaf, branch and even in raindrop collected from prune orchard. Pre-incubation along with nested PCR enhanced sensitivity to detect X. arboricola pv. pruni from seemingly healthy looking, symptomless branches. Designed integrons PCR can be used in prune nursery fields and in plant quarantine practice for the detection of X. arboricola pv. pruni.
Keywords
Integrons; Nested PCR; PCR detection; Prunus; Xanthomonas arboricola pv. pruni;
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