• Title/Summary/Keyword: Sporophore

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Effects of Some Environmental Factors on the Mycelial Growth and Mushroom Yield of Agaricus bisporus (양송이 균사(菌絲) 생장(生長)과 자실체(子實體) 수량(收量)에 미치는 몇가지 환경요인(環境要園)의 영향(影響))

  • Cha, Dong-Yeul;Park, Jong-Seong;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.9 no.1
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    • pp.7-12
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    • 1981
  • Attempts were made to investigate the effects of the temperature and carbon dioxide concentration in the growing room and soil moisture after casing on the mycelial growth, sporophore formation and mushroom yield of Agaricus bisporus (Lange) Sing. The growing room temperature influenced the mycelial growth in the casing layer after casing and the sporophore formation of Agaricus bisporus. The mycelial growth was the rapidest at $30^{\circ}C$ and gradually decreased with the temperature going down, while the sporophore formation and mushroom yield were the best at $25^{\circ}C$. The other factor which affected the mycelial growth and sporophore formation was the moisture content of casing soil. The mycelial growth was the best at 70 percent moisture, and the sporophore formation and mushroom yields were the highest at 60 percent moisture. The concentration of carbon dioxide in the growing room after casing had an important effect upon the mycelial growth in the casing layer and the sporophore formation. When the concentration of carbon dioxide was 0.16 percent, the mycelial growth and the sporophore formation were not inhibited. At 0.5 to 2.0 percent $CO_{2}$ the myceilal growth and the sporophore formation were severely decreased. The sporophore size of the mushroom was the maximum when the room temperature during the vegetative mycelium growth was $20^{\circ}C$ and the moisture content of casing soil was 70 percent.

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Nutritional Physiology and improvement of substrate of Lentinus edodes (표고 버섯(Lentinus edodes)의 영양생리 및 기질개발)

  • Park, Won-Mok;Song, Chi-Hyeun;Hyun, Jae-Wook
    • The Korean Journal of Mycology
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    • v.20 no.1
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    • pp.77-82
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    • 1992
  • Researches were carried out to find the optimal conditions of carbon sources, nitrogen sources and pH for the maximum production of sporophore of Lentinus edodes. Dextrin, aspartic acid and pH 4.0 were the best conditions for yield of sporophore by using replacement culture technique. The production of sporophore was stimulated by addition of 0.8% triacylglycerol in NS medium. Coffee waste was chosen for the best substrate among the poplar, oak, white aspen saw dust and coffee waste. Increased growth of mycelim and yield of sporophore was obsewed by adding tannin up to 0.1% as substrate.

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Application of Super Water Absorbent for Edible Mushroom Production (식용버섯 재배시 초고흡수성 폴리머의 효과)

  • Kim, Myung-Kon;Yoon, Sook;Mun, Sung-Pil;Kim, Hyung-Moo;Chang, Tae-Bok;Hong, Jae-Sik
    • The Korean Journal of Mycology
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    • v.28 no.1
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    • pp.16-21
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    • 2000
  • Super water absorbent (CPAM-AS-hyd-1) was prepared by polymerization of acrylamide and allyl sulfonate salt with N,N'-methylene-bis-acrylamide as crosslinking agent, followed by alkaline hydrolysis and the effect on mycelial growth and sporophore production of edible mushrooms in the artificial cultivation was examined. The mycelial growth of edible mushrooms did not depend on the addition of super water absorbent upto 200 g of hydrated polymer gel per 100 cc medium. The proper hydrated polymer gel concentration for sporophore production of Pleurotus sajor-caju and Hericium erinaceus were 200 g and $200{\sim}250\;g$ per 100 cc medium, respectively. The proper hydrated polymer gel and puffed rice hull concentration for sporophore production of Flammulina velutipes was 200 g per 100 mm medium and 10% (v/v), respectively.

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Development of Substrate and Cultural Method for the Cultivation of Pleurotus sajor-caju (느타리 버섯(Pleurotus sajor-caju) 재배를 위한 기질 및 재배방법의 개발)

  • Hong, B.S.;Kim, S.J.;Song, C.H.;Hwang, S.Y.;Yang, H.C.
    • The Korean Journal of Mycology
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    • v.20 no.4
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    • pp.354-359
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    • 1992
  • The effect of the addition of various vegetable oils on the mycelial growth was studied. Most vegetable oils were proved to be stimulative for the mycelial growth, and the best mycelial growth (12 mg/ml) was obtained with the addition of cotton seed oil. Several agricultural wastes i.e., rice straw, peanut hull, sawdust, rice hull, cocoa hull, coffee waste and beer waste were empolyed as substrates for sporophore production of p. sajor-caju. The biological efficiency(BE) for sporophore productions of rice straw and peanut hull were 36.4% and 32.6%, respectively. The highest yield of sporophore was obtained from the mixture of rice straw (50%) and beer waste (50%)(BE 109.6%) followed by peanut hull (50%) and beer waste (50%)(BE; 74.5%).

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An investigation of insect pest on the Mushroom (Lentinus edode, Pleurotus ostreatus) in south region of Korea.) (한국남부 표고버섯 및 느타리버섯 재배지에 분포된 해충상에 관한 연구)

  • 김규진;황창연
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.45-51
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    • 1996
  • As the pest of the Letinus edoeds, five insect species and six animal species are investigated injuring the fresh mushroom. Of these, insect and animals, Achorutes armatus, Scaphidium amurense and Philomycus confusa caused severe damage to mushroom fruitbody, and a greate deal of damage was done to mushroom lamella by mites (Rhizoglyphis sp., and Histiostoma sp.). As the pest of Lentinus edodes bed logs, two insect species from immature bed logs, eight insects and one isopod species from matured bed logs were investigated. Of those, Moechotypa diphysis was found to be the most injurious species from both immature and matured bed logs. As the pest of Pleurotus ostreatus, six insects and the two mite species were investigated from sporophore. Of those, Lycoriella sp. and Mycophilla sp. are considered to be very serious. while two insects and five mite species were investigated from the mushroom bed. Of those, one insect (Lycoriella sp.) and two mites (Histiostoma feroniarum and Throglyphus bankslogior) found to be abundant and caused serious damage.

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Studies on the Chemical Control of Mycogone perniciosa Magn in Cultivation of Mushroom Agaricus bisporus(Lang) Sing (양송이 마이코곤병(病)의 방제약제(防除藥劑)에 관한 연구(硏究))

  • Jhune, Chang-Sung;Kim, Gwang-Po;Cha, Dong-Yeul
    • The Korean Journal of Mycology
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    • v.19 no.1
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    • pp.85-87
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    • 1991
  • Prochloraz of fungicide was applied on Mycogone perniciosa causing wet bubble in cultivated mushroom, Agaricus bisporus. In vitro, Prochloraz was an excellent fungicide on two strains of Mycogone, tolerant and non-tolerant to Benomyl, respectively. At the low dosage, Prochloraz more inhibited mycelial growth of mushrooms than Benomyl. At the higher dosage, Benomyl more inhibited the mycelial growth than prochloraz. The higher yield of sporophore of the mushroom with low inferction rate was abtained from several trial of Prochloraz. Prochloroz was concluded to be effective fungicide on Mycogone perniciosa on Agaricus cultivation.

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An Improved Compost Using Cotton Waste and Fermented Sawdust Substrate for Cultivation of Oyster Mushroom

  • Oh, Se-Jong;Park, Jeong-Sik;Shin, Pyung-Gyun;Yoo, Young-Bok;Jhune, Chang-Sung
    • Mycobiology
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    • v.32 no.3
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    • pp.115-118
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    • 2004
  • A composting technique was assessed to enhance efficiency in oyster mushroom substrate. Poplar sawdusts and by-product of winter mushroom added by 10% of rice bran were composted outdoors at $20^{\circ}C$ to $28^{\circ}C$ for 12 days. The duration of fermentation was shortened 3 days in fermented sawdust plot. The yield obtained from waste cotton substrate with fermented poplar sawdust was 742 g, whereas the yield from control 663 g. In addition, the substrate with by-product of winter mushroom was the most effective to composting. It was useful to apply the fermented sawdust and by-product to waste cotton for compost and sporophore production of oyster mushroom.

Identification and genetic characterization of bacterial isolates causing brown blotch on cultivated mushrooms in Korea

  • Chan-Jung Lee;Hye-Sung Park;Seong-Yeon Jo;Gi-Hong An;Ja-Yun Kim;Kang-Hyo Lee
    • Journal of Mushroom
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    • v.22 no.2
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    • pp.37-47
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    • 2024
  • Fluorescent bacteria were isolated from sporocarps that browned into various mushrooms during survey at places of the production in Korea. We examined the pathogenicity, biodiversity, and genetic characteristics of the 19 strains identified as Pseudomonas tolaasii by sequence analysis of 16S rRNA and White Line Assay. The results emphasize the importance of rpoB gene system, fatty acid profiles, specific and sensitive PCR assays, and lipopeptide detection for the identification of P. tolaasii. As a result of these various analyses, 17 strains (CHM03~CHM19) were identified as P. tolaasii. The phylogenetic analysis based on the 16S rRNA gene showed that all strains were clustered closest to P. tolaasii lineage, two strains (CHM01, CHM02) were not identified as P. tolaasii and have completely different genetic characteristics as a result of fatty acids profile, specific and sensitive PCR, lipopetide detection, rpoB sequence and REP-PCR analysis. Pathogenicity tests showed 17 strains produce severe brown discolouration symptoms to button mushrooms and watersoaking of sporophore tissue within three days after inoculation. But two strains did not produce discolouration symptoms. Therefore, these two strains will be further investigated for correct species identification by different biological and molecular characteristics.

Physiological Characteristics of Actinomycetes Isolated in the Upland Soils (밭 토양에서 분리한 방선균의 생리적 특성)

  • Park, Kyoung-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.4
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    • pp.394-400
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    • 1992
  • In the isolated 280 Actinomycetes strains, 12 groups of Streptomyces were 87.2% and 3groups of Non-Streptomyces were 12.8%, respectively. Streptomyces with sporophore of the spiral chain form reached to 80% of all the Streptomyces isolates. Surface morphology of spores have been determined with the electron microscope ; two groups have a spiny surface, 10 groups have a smooth surface. Isolated Actinomyceles groups were indentified as Streptomycetes groups and Non-Streptomyces groups. Actinomycetes isolates were selected as the strains having predominant antibacterial activities against the microorganisms among the 15 groups which has antibacterial activities. Selected Actinomycetes isolates showed high antibiotic sensitivity of S-9 strain(8.46 r/ml), S-6 strain(6.23 r/ml), S-2 strain(7.24 r/ml) against Pseudomonas aeruginosa (ATCC 27857), Escherichia coli (ATCC 25922) and Staphylococcus aureus(ATCC 6538).

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Sceptridium atrovirens and S. microphyllum (Ophioglossaceae): First report in Korea and its phylogenetic position based on morphology (숲고사리삼과 애기고사리삼(고사리삼과): 한반도 미기록 식물의 형태로 본 계통적 위치)

  • Lee, Chang Shook;Kim, Yoo Sung
    • Korean Journal of Plant Taxonomy
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    • v.42 no.1
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    • pp.68-75
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    • 2012
  • Two unrecorded species (Sceptridium atrovirens and S. microphyllum), previously known only in Japan, were found in a forest in Dosun-dong, Seogwipo-si, Jeju-do for the first time in Korea. S. atrovirens was distinguished from other related species of the genus Sceptridium in Korea by having the thickest rhizome and root, irregularly crenulate or roughly serrate, and a shorter sporophore blade. The local name was newly given as 'Sup-go-sa-ri-sam' for the species, denoting the habitat. S. microphyllum was distinguished by having the smallest plant height, compact sterile blades, and partly lateritious tropophyll in winter. The local name was newly given as 'Aegi-go-sa-ri-sam' for species, meaning of a small size. To reveal the interspecific relationships within the genus Sceptridium in Korea, cladistic analysis was performed for 27 morphological characteristics. The morphological characteristics and illustrations of the two taxa, as well as photographs at the habitat, are provided with a taxonomic key to the species of Sceptridium in Korea.