Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Identification and genetic characterization of bacterial isolates causing brown blotch on cultivated mushrooms in Korea

  • Chan-Jung Lee (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hye-Sung Park (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Seong-Yeon Jo (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Gi-Hong An (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Ja-Yun Kim (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kang-Hyo Lee (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • Received : 2024.05.07
  • Accepted : 2024.06.24
  • Published : 2024.06.30
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Abstract

Fluorescent bacteria were isolated from sporocarps that browned into various mushrooms during survey at places of the production in Korea. We examined the pathogenicity, biodiversity, and genetic characteristics of the 19 strains identified as Pseudomonas tolaasii by sequence analysis of 16S rRNA and White Line Assay. The results emphasize the importance of rpoB gene system, fatty acid profiles, specific and sensitive PCR assays, and lipopeptide detection for the identification of P. tolaasii. As a result of these various analyses, 17 strains (CHM03~CHM19) were identified as P. tolaasii. The phylogenetic analysis based on the 16S rRNA gene showed that all strains were clustered closest to P. tolaasii lineage, two strains (CHM01, CHM02) were not identified as P. tolaasii and have completely different genetic characteristics as a result of fatty acids profile, specific and sensitive PCR, lipopetide detection, rpoB sequence and REP-PCR analysis. Pathogenicity tests showed 17 strains produce severe brown discolouration symptoms to button mushrooms and watersoaking of sporophore tissue within three days after inoculation. But two strains did not produce discolouration symptoms. Therefore, these two strains will be further investigated for correct species identification by different biological and molecular characteristics.

Keywords

Acknowledgement

This study was supported by research grant from the Rural Development Administration(RDA) of Korea.

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