• 대한안전경영과학회지
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• 제12권4호
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• pp.81-86
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• 2010

Despite the importance of cycling postures during cycling performances, there has been a very little research investigating cycling postures and pedaling rate for particularly concerning domestic cyclists. The aim of this study was to analyze correlations and effects between cycling postures and pedaling rate in track cycling. Twelve male racing cyclists (six racing and university cyclists) participated in this research. For this study, seven infrared cameras (Qualisys ProReflex MCU-240s) were used for collecting data and these were processed via QTM (Qualisys Tracker Manager) software. It appeared that pedaling rate had correlations with regard to a shoulder angle (R=-.601) and displacement between shoulder joints(R= -.637), but a knee (R=-.601) and ankle angle (R=.667). Moreover, two multiple regression equations of pedaling rate for cycling postures were significant and R2 of the first order equation y (pedaling rate) = 0.039x (knee angle) - 1.068 was less than the second order equation y = 0.006x2 - 1.287x + 69.674. In conclusion, cycling postures affected the pedaling rate. Further study should be researched on postures in relation to air resistance in a wind tunnel.

• 환경생물
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• 제22권4호
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• pp.487-493
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• 2004

To investigate the antifungal activity related protein in pesticidal bacteria, a bacterial strain LTD was isolated from soil collected at Gimje in Jeonbuk province, Korea, and identified as Bacillus subtilis LTD based on a API50 CHB kit and 168 rDNA sequencing. It has an antifungal activity against 9 plant pathogenic fungi in a paper disc assay. The antifungal activity- deficient mutant, B. subtilis mLTD was induced at a 5 kGy dose of $^{60}Co$ gamma radiation. Using the two-dimensional electrophoresis and the matrix assisted laser desorption ionization time-of-flight mass spectrometry, the comparison analysis of proteins between the wild and mutant were performed. A major intracellular serine proteinase IspA (MW: 32.5 kDa), a NAD (P) H dehydrogenase (MW: 20.0 kDa), and a stage II sporulation protein AA, SpoIIAA (MW: 14.3kDa) were detected only in the B. subtilis LTD. These results suggested that the functions of these proteins found only in the B. subtilis LTD could. be closely related to the antifungal activity against plant pathogenic fungi.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권5호
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• pp.432-443
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• 2005

Microarray technology has contributed Significantly to the understanding of bacterial genetics and transcriptional regulation. One neglected aspect of this technology has been optimization of microarray-generated signals and quality of generated information. Full genome microarrays were developed for Clostridium acetobutylicum through spotting of PCR products that were designed with minimal homology with all other genes within the genome. Using statistical analyses it is demonstrated that Signal quality is significantly improved by increasing the hybridization volume. possibly increasing the effective number of transcripts available to bind to a given spot, while changes in labeled probe amounts were found to be less sensitive to improving signal quality. In addition to Q-RT-PCR, array validation was tested by examining the transcriptional program of a mutant (M5) strain lacking the pSOL1 178-gene megaplasmid relative to the wildtype (WT) strain. Under optimal conditions, it is demonstrated that the fraction of false positive genes is 1% when considering differentially expressed genes and 7% when considering all genes with signal above background. To enhance genomic-scale understanding of organismal physiology, using data from these microarrays we estimated that $40{\sim}55%$ of the C. acetobutylicum genome is expressed at any time during batch culture, similar to estimates made for Bacillus subtilis.

• 한국수산과학회지
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• 제9권1호
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• pp.35-42
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• 1976

1974년부터 1975년 말까지 부안만 대합에 기생하는 흡충류 유충의 발육, 기생율, 조직변화 및 사육실험을 통하여 대합의 폐사원인을 밝혔다. 1. 대합의 생식소, 중장선, 새엽에 기생하는 유모 cercaria인 Cercaria pectinata Huet와 그 sporocyst를 검출하였다. 2. 기생충의 수와 량이 급히 증가하므로 흡충류에 기생된 대합은 기능장해를 받아 폐사된다. 3. 부안산 대합 2,639개체에 대한 대합에 기생되는 흡충의 평균 기생율은 $13.2\%$였는데, 월별로 보면 7월이 가장 높았으며$(29.6\%)$, 3월이 가장 낮았다$(2.0\%)$. 한편 각장에 관계없이 기생율은 거의 같았다. 4. 흡충류에 기생된 대합의 생식소는 거세되고, 유충의 증식으로 중장선은 압박을 받으며 새판내에 기생되므로 혈액순환장해를 일으킨다. 5. 수조 내에서 사육한 기생패는 35일만에 전부 폐사했다.

• 디지털융복합연구
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• 제17권8호
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• pp.197-204
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• 2019

본 연구의 목적은 스마트폰 사용자들의 의사결정에 영향을 미치는 요소들을 탐구하는 것이었다. 본 연구는 친밀감, 개인연결성, 사용 및 의존성, 친숙함 및 전문성, 효능 및 효과, 자아정체성, 제어 등을 독립 변수로 설정하고 의사 결정을 종속 변수로 설정한다. 본 연구에서는 스마트폰 사용자 200명을 설문지를 통해 수집 분석하였으며, 변수간 영향을 파악하기 위해 Smartpls 3.0을 사용하였다. 스마트폰 사용자의 심리적 강도에 영향을 준 6가지 요인 중 5가지가 의사결정에 영향을 미친 것으로 분석됐다. 또한 조절변수로서의 사용 기간 등이 심리적 영향과의 관련성도 살펴보았다. 본 연구 분석 결과는 이전에 스마트폰의 심리적 영향에 대한 요인들에 연구가 미진한 가운에 스마트폰 기기의 발전 형태의 소비자 행동 기반이 될 수 있고, 향후 본 연구를 바탕으로 스마트폰 시스템 개발에 도움이 될 것으로 기대한다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제34권6호
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• pp.449-454
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• 2012

Purpose: Intravenous sedation with midazolam is common in contemporary dentistry. That is effective for anxious patients, but additional analgesic agent needs to be used, because midazolam alone doesn't have an analgesic effect. This study was performed to select an analgesic agent between an opioid agent, and nonsteroidal anti-inflammatory drugs as adjunctives in intravenous sedation with midazolam. Methods: The subjects were 60 patients who visited the Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University, between August 2009 and February 2010. Conscious sedation was performed on 20 patients of 3 groups (control group, ketorolac group, and fentanyl group), who were divided randomly. The analgesic agent was administrated preoperatively. For sedation, vital signs were recorded. After sedation and operation, subjective questionnaires of the patient and operator were implemented. Results: All of the $SPO_2$, blood pressure, and heart rates stayed within the normal range for sedation. The sedation depth and analgesic effect of the ketorolac group and fentanyl group were similar. In the case of sedation depth, 12 patients in the ketorolac group and 14 patients in the fentanyl group had no memory of surgery. In the case of analgesic effect, the visual analogue scale of pain scored 2~3 in 13 patients in the ketorolac group, and 0~2 in 12 patients in the fentanyl group. The satisfaction of patients and doctors was also similar. Conclusion: Considering the management and complication of an opioid agent, non-steroidal anti-inflammatory drugs is more effective than an opioid agent.

• 대한전자공학회논문지TC
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• 제42권12호
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• pp.127-134
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• 2005

VoIP에서 패킷의 손실은 음성의 품질에 영향을 주는 가장 중요한 요인이다. 따라서 수신된 정보로부터 손실된 패킷을 복구하는 것은 중요하다. 따라서 본 논문은 VoIP에서 가장 많이 사용되는 CELP 부호화기를 위한 수신측 기반의 손실 패킷 복구방법을 제안한다. 제안하는 WSOLA(Waveform Shift OverLab Add)기반의 BS-PLC (Both Side Packet Loss Concealment) 방법은 패킷 손실이 발생하였을 경우 미래 패킷을 이용할 수 있는 경우와 그렇지 않을 경우로 나누고, LP(Linear prediction) 파라미터와 여기 신호를 복구한다. 미래 패킷을 이용할 수 없는 경우에는 과거에 전송된 정상 패킷만을 가지고 복원을 하며, 미래 패킷을 이용할 수 있을 경우에는 과거의 정상 패킷과 미래의 정상 패킷을 동시에 이용하여 손실된 패킷을 복구한다. 연속 패킷 손실 환경은 Gilbert 모델로 설정하였고, 제안한 알고리즘을 VoIP에서 가장 많이 사용되는 CELP 음성부호화기인 G.729에 적용하여 성능을 비교한다. 성능 비교를 위해 손실율을 변화시키면서 SNR(Signal to Noise)와 MOS(Mean Opinion Score)측정하였고, 제안한 방법을 G.729의 패킷 손실 은닉 방법과 비교하였다. 실험 결과, 평균 손실률이 $20\%$에서도 SNR은 2dB, MOS값은 0.3정도로의 음질 개선을 보였다.

• 감성과학
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• 제13권1호
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• pp.41-46
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• 2010

본 연구는 93% 고농도 산소의 세 가지 공급량(1L/min, 3L/min, 5L/min)에 대해 20대 및 60대의 혈중산소 포화도의 차이를 규명하고자 하였다. 20대 남자 10명($25.0{\pm}1.8$세)과 여자 10명($23.7{\pm}1.9$세), 60대 남자 10명($68.0{\pm}2.6$세)과 여자 10명($65.5{\pm}3.1$세)의 피험자를 대상으로 실험을 수행하였다. 1L/min, 3L/min, 그리고 5L/min의 유량 변화가 가능하면서 순도 93%의 산소 농도를 일정하게 유지 할 수 있는 산소 공급 장치(OXUS Co.)를 사용하였다. Pre-hyperoxia(5분), 93%의 Hyperoxia(10분), Post-hyperoxia(10분)의 세 단계의 순서로 실험이 진행되었고, 전 단계에서 혈중 산소 포화도가 측정되었다. 유량이 증가할수록 혈중 산소 포화도는 증가하였고, 60대보다 20대의 혈중 산소 포화도가 더 높았다. 안정 상태(Pre-/Post-hyperoxia)에 비해 고농도 산소를 공급한 Hyperoxia 구간에서 혈중 산소 포화도가 증가하였다. 그러나 고농도 산소 공급으로 20대에 비해 60대의 혈중 산소 포화도의 증가량이 더 컸다.

• Interdisciplinary Bio Central
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• 제1권1호
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• pp.3.1-3.6
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• 2009

Introduction: GTPases known as translation factor play a vital role as ribosomal subunit assembly chaperone. The bacterial Obg proteins ($Spo{\underline{0B}}$-associated ${\underline{G}}TP$-binding protein) belong to the subfamily of P-loop GTPase proteins and now it is considered as one of the new target for antibacterial drug. The majority of bacterial Obgs have been commonly found to be associated with ribosome, implying that these proteins may play a fundamental role in ribosome assembly or maturation. In addition, one of the experimental evidences suggested that Bacillus subtilis Obg (BsObg) protein binds to the L13 ribosomal protein (BsL13) which is known to be one of the early assembly proteins of the 50S ribosomal subunit in Escherichia coli. In order to investigate binding mode between the BsObg and the BsL13, protein-protein docking simulation was carried out after generating 3D structure of the BsL13 structure using homology modeling method. Materials and Methods: Homology model structure of BsL13 was generated using the EcL13 crystal structure as a template. Protein-protein docking of BsObg protein with ribosomal protein BsL13 was performed by DOT, a macro-molecular docking software, in order to predict a reasonable binding mode. The solvated energy minimization calculation of the docked conformation was carried out to refine the structure. Results and Discussion: The possible binding conformation of BsL13 along with activated Obg fold in BsObg was predicted by computational docking study. The final structure is obtained from the solvated energy minimization. From the analysis, three important H-bond interactions between the Obg fold and the L13 were detected: Obg:Tyr27-L13:Glu32, Obg:Asn76-L13:Glu139, and Obg:Ala136-L13:Glu142. The interaction between the BsObg and BsL13 structures were also analyzed by electrostatic potential calculations to examine the interface surfaces. From the results, the key residues for hydrogen bonding and hydrophobic interaction between the two proteins were predicted. Conclusion and Prospects: In this study, we have focused on the binding mode of the BsObg protein with the ribosomal BsL13 protein. The interaction between the activated Obg and target protein was investigated with protein-protein docking calculations. The binding pattern can be further used as a base for structure-based drug design to find a novel antibacterial drug.