• 조명전기설비학회논문지
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• 제29권12호
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• pp.1-7
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• 2015

In this paper, we propose a second-order fiber flexible comb filter based on a polarization-diversity loop(PDL). The proposed filter consists of a polarization beam splitter, four half-wave plates(HWPs), and three high birefringence fiber(HBF) segments. In the previous Solc-type second-order filter based on the PDL, HBF segments were fusion-spliced with a fixed angle offset between their principal axes with each other. But, the proposed filter implemented by inserting two HWPs between three HBF segments has a great flexibility in adjusting relative angular difference between the principal axes of two adjacent HBF segments. Owing to this flexibility, second-order transmission spectra, which had a channel spacing of ~0.8nm, could be interleaved by controlling the orientation angles of four HWPs. The output transmission spectra of the proposed filter were theoretically analyzed and experimentally verified.

• 한국지진공학회논문집
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• 제20권6호
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• pp.351-360
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• 2016

In regions of low-to-moderate seismicity, various types of lap splices are used for longitudinal reinforcement of columns at the plastic hinge zones. The seismic performance of such lap spliced columns, such as strength, deformation capacity, and energy dissipation, is affected by material strengths, longitudinal re-bar size, confinement of hoops, lap splice location, and lap splice length. In the present study, cyclic loading tests were performed for columns using three types of lap splices (bottom offset bar splice, top offset bar splice, and splice without offset bend). Lap splice length($40d_b$ and $50d_b$) was also considered as test parameters. Ties with 90-degree end hooks were provided in the lap splice length. The test results showed that strength, deformation capacity, and energy dissipation of columns significantly differed depending on the details and the length of lap splices. The bottom offset bar splice showed high ductility and energy dissipation but low strength; on the other hand, the top offset bar splice and the splice without offset bend showed high strength but moderate ductility and energy dissipation.

• Structural Engineering and Mechanics
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• 제17권1호
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• pp.99-112
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• 2004

It is known that lap splices in the longitudinal reinforcement of reinforced concrete (RC) bridge columns are not desirable for seismic performance, but it is sometimes unavoidable. Lap splices were practically located in the potential plastic hinge region of most bridge columns that were constructed before the 1992 seismic design provisions of the Korea Bridge Design Specification. The objective of this research is to evaluate the seismic performance of reinforced concrete (RC) bridge piers with lap splicing of longitudinal reinforcement in the plastic hinge region, to develop an enhancement scheme for their seismic capacity by retrofitting with glassfiber sheets, and to assess a damage of bridge columns subjected to seismic loadings for the development of rational seismic design provisions in low or moderate seismicity region. Nine (9) test specimens with an aspect ratio of 4 were made with three confinement ratios and three types of lap splice. Quasi-static tests were conducted in a displacement-controlled way under three different axial loads. A significant reduction of displacement ductility was observed for test columns with lap splices of longitudinal reinforcements, whose displacement ductility could be greatly improved by externally wrapping with glassfiber sheets in the plastic hinge region. A damage of the limited ductile specimen was assessed to be relatively small.

• Biotechnology and Bioprocess Engineering:BBE
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• 제3권1호
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• pp.11-14
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• 1998

A toluene-oxidizing strain of Pseudomanas mendocina KR1 containing toluene-4-mono-oxygenase (TMO) completely degrades TCE with the addition of toluene as a co-substrate in aerobic condition. In order to construct in situ bioremediation system for TCE degradation without any growth-stimulating nutrients or toxic inducer such as toluene, we used the carbon-starvation promoter of Pseudomonas putida MK1 (Kim, Y. et al., J. bacteriol., 1995). Upon entry into the stationary phase due to the deprivation of nutrients, this promoter is strongly induced without further cell growth. The TMO gene cluster (4.5 kb) was spliced downstream of the carbon starvation promoter of Pseudomonas putida MK1, already cloned in pUC19. TMO under the carbon starvation promoter was not expressed in E. coli cells either in stationary phase or exponential phase. For TMO expression in Pseudomonas strains, tmo and carbon starvation promoter region were recloned into a modified broad-host range vector pMMB67HES which was made from pMMB67HE(8.9 kb) by deletion of tac promoter and lacIq (about 1.5 kb). Indigo was produced by TMO under the carbon starvation promoter in a Pseudomonas strain of post-exponential phase on M9 (0.2% glucose and 1mM indole) or LB. 18% of TCE was degraded in 14 hours after entering the stationary phase at the initial concentration of 6.6 ${\mu}$M in liquid phase.

• Molecules and Cells
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• 제21권1호
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• pp.21-28
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• 2006

The interferon-induced, double-stranded RNA (dsRNA)-dependent protein kinase PKR plays a key role in interferon-mediated host defense against viral infection, and is implicated in cellular transformation and apoptosis. We have isolated a cDNA of simian PKR encoding a product with 83% amino acid identity to the human homolog and showed that PKR expression is significantly attenuated in the Vero E6 African green monkey kidney cells devoid of type I interferon genes. A variant form of PKR lacking the exon 12 in the kinase domain is produced in these cells, presumably from an alternatively spliced transcript. Unlike wild type PKR, the variant protein named PKR-${\Delta}E12$ is incapable of auto-phosphorylation and phosphorylation of eIF2-${\alpha}$, indicating that the kinase sub-domains III and IV embedded in exon 12 are indispensable for catalytic function. PKR-${\Delta}E12$ had no dominant negative effect but was weakly phosphorylated in trans by wild type PKR.

• Computers and Concrete
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• 제16권2호
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• pp.245-260
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• 2015

In this study, nominal moment-axial load interaction diagrams, moment-curvature relationships, and ductility of rectangular hybrid beam-column concrete sections are analyzed using the modified Hognestad concrete model. The hybrid columns are primarily reinforced with steel bars with additional Glass Fiber Reinforced Polymer (GFRP) control bars. Parameters investigated include amount, pattern, location, and material properties of concrete, steel, and GFRP. The study was implemented using a user defined comprehensive $MATLAB^{(R)}$ simulation model to find an efficient hybrid section design maximizing strength and ductility. Generating lower bond stresses than steel bars at the concrete interface, auxiliary GFRP bars minimize damage in the concrete core of beam-column sections. Their usage prevents excessive yielding of the core longitudinal bars during frequent moderate cyclic deformations, which leads to significant damage in the foundations of bridges or beam-column spliced sections where repair is difficult and expensive. Analytical results from this study shows that hybrid steel-GFRP composite concrete sections where GFRP is used as auxiliary bars show adequate ductility with a significant increase in strength. Results also compare different design parameters reaching a number of design recommendations for the proposed hybrid section.

• BMB Reports
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• 제54권7호
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• pp.335-343
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• 2021

Cancer stem cells (CSCs) are a subpopulation of cancer that can self-renew and differentiate into large tumor masses. Evidence accumulated to date shows that CSCs affect tumor proliferation, recurrence, and resistance to chemotherapy. Recent studies have shown that, like stem cells, CSCs maintain cells with self-renewal capacity by means of asymmetric division and promote cell proliferation by means of symmetric division. This cell division is regulated by fate determinants, such as the NUMB protein, which recently has also been confirmed as a tumor suppressor. Loss of NUMB expression leads to uncontrolled proliferation and amplification of the CSC pool, which promotes the Notch signaling pathway and reduces the expression of the p53 protein. NUMB genes are alternatively spliced to produce six functionally distinct isoforms. An interesting recent discovery is that the protein NUMB isoform produced by alternative splicing of NUMB plays an important role in promoting carcinogenesis. In this review, we summarize the known functions of NUMB and NUMB isoforms related to the proliferation and generation of CSCs.

• BMB Reports
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• 제55권12호
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• pp.639-644
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• 2022

Serine-arginine-rich splicing factors (SRSFs) are members of RNA processing proteins in the serine-arginine-rich (SR) family that could regulate the alternative splicing of the human immunodeficiency virus-1 (HIV-1). Whether SRSF9 has any effect on HIV-1 regulation requires elucidation. Here, we report for the first time the effects and mechanisms of SRSF9 on HIV-1 regulation. The overexpression of SRSF9 inhibits viral production and infectivity in both HEK293T and MT-4 cells. Deletion analysis of SRSF9 determined that the RNA regulation motif domain of SRSF9 is important for anti-HIV-1 effects. Furthermore, overexpression of SRSF9 increases multiple spliced forms of viral mRNA, such as Vpr mRNA. These data suggest that SRSF9 overexpression inhibits HIV-1 production by inducing the imbalanced HIV-1 mRNA splicing that could be exploited further for a novel HIV-1 therapeutic molecule.

• 한국광학회지
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• 제8권4호
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• pp.333-339
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• 1997

클래딩 지름이 다른 광섬유 브래그 격자를 용융 접착하여 스트레인과 온도를 분리하여 측정할 수 있는 센서를 구성하였다. 굵기차에 의해서 스트레인에 대한 각 브래그 파장 변화량은 서로 다르며, 동일한 모재(perform)에서 생산된 광섬유를 사용하였으므로 온도에 대한 파장 변화량은 같았다. 두 브래그 파장의 변화량을 측정하고 잘 정의된 행렬함수에 대입하여, 가해진 스트레인과 온도의 양을 분리하여, 계산할 수 있었다. 0-1500 .mu.strain, 20-100.deg. C 범위의 스트레인과 온도 변화를 가하면서 제작된 센서의 특성을 관측하였고, 브래그 파장 변화를 측정하여 계산한 결과, 온도계와 마이크로미터 값에 비하여 10% 이내의 측정오차를 얻을 수 있었다. 스트레인의 측정 정일도를 높이기 위해서 일정한 광경로차를 갖는 마하젠더 간섭계를 이용하여 두 격자의 상대 파장변화를 간섭 신호의 크기변화로 변환하는 새로운 방법을 제안하였으며, 시스템을 구축하여 실험한 결과로 온도에 무관하게 분광분석기에 비항 80배 이상 향상된 스트레인 측정 정밀도를 얻을 수 있었다.

• 말소리와 음성과학
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• 제2권3호
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• pp.115-124
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• 2010

The current study investigated the role of acoustic correlates of domain-initial strengthening in lexical segmentation of a non-native language. In a series of cross-modal identity-priming experiments, native Korean listeners heard English auditory stimuli and made lexical decision to visual targets (i.e., written words). The auditory stimuli contained critical two word sequences which created temporal lexical ambiguity (e.g., 'mill#company', with the competitor 'milk'). There was either an IP boundary or a word boundary between the two words in the critical sequences. The initial CV of the second word (e.g., [$k_{\Lambda}$] in 'company') was spliced from another token of the sequence in IP- or Wd-initial positions. The prime words were postboundary words (e.g., company) in Experiment 1, and preboundary words (e.g., mill) in Experiment 2. In both experiments, Korean listeners showed priming effects only in IP contexts, indicating that they can make use of IP boundary cues of English in lexical segmentation of English. The acoustic correlates of domain-initial strengthening were also exploited by Korean listeners, but significant effects were found only for the segmentation of postboundary words. The results therefore indicate that L2 listeners can make use of prosodically driven phonetic detail in lexical segmentation of L2, as long as the direction of those cues are similar in their L1 and L2. The exact use of the cues by Korean listeners was, however, different from that found with native English listeners in Cho, McQueen, and Cox (2007). The differential use of the prosodically driven phonetic cues by the native and non-native listeners are thus discussed.