• Korean Journal of Medicinal Crop Science
• /
• v.24 no.1
• /
• pp.31-37
• /
• 2016

Background : We studied the anti-oxidant activity and anti-inflammatory effects of Spiraea fritschiana Schneid extract (SFSE). Methods and Results : The SFSE was prepared using methanol and was evaluated for its total phenol and flavonoid content, DPPH (1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity, reducing power, and effect on nitric oxide (NO) production, and cell viability by using real-time polymerase chain reaction (PCR). The total phenol content was $212.78{\mu}g{\cdot}galli$c acid equivalent (GAE)/mg and the total flavonoid content was $66.84{\mu}g{\cdot}quercetin$ equivalent (QE)/mg. The extract showed antioxidant activity (DPPH free-radical scavenging activity) with $RC_{50}$ value of $76.61{\mu}g/m{\ell}$. The reducing power of the extract was Abs 0.58 at $250{\mu}g/m{\ell}$. Cell viability was determined using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. To evaluate anti-inflammatory activity, we examined the inhibitory effects on lipopolysaccharide-(LPS)-induced NO production in RAW 264.7 cells. The NO inhibition rate was 90% at $200{\mu}g/m{\ell}$ SFSE. At the same concentration, the expression of pro-inflammatory genes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 also decreased. Conclusions : Our results suggest that SFSE is a novel resource for the development of foods and drugs that possess anti-oxidant and anti-inflammatory activity.

• Journal of Life Science
• /
• v.19 no.9
• /
• pp.1183-1189
• /
• 2009

85 individual Spiraea prunifolia for. simpliciflora (Rosaceae) were sampled to examine the genetic diversity and population structure of S. prunifolia for. simpliciflora populations. Inter-simple sequence repeats (ISSR) produced 65 polymorphic loci and identified 78 ISSR genotypes. Three multilocus genotypes were shared by more than one plant within a population. Total genetic diversity values ($H_T$) and inter-locus variation in the within-population genetic diversity ($H_S$) were 0.293 and 0.183, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.373. This indicated that about 37.3% of the total variation was among populations. ISSR markers are very effective in classifying natural population levels of S. prunifolia for. simpliciflora in Korea. In addition, insights into the relative gene diversity among and within populations of S. prunifolia for. simpliciflora would be useful in plant breeding and also for the development of strategies for ex situ conservation of plant genetic resources.

• Korean journal of applied entomology
• /
• v.34 no.2
• /
• pp.89-94
• /
• 1995

Resistance mechanism of organophosphorus insecticides (OPs) in the spiraea aphid, whose populations were originated from several apple orchards where various OPs were frequently sprayed, was investigated. For Wonju population to which insecticides were not sprayed, resistance ratios (BRs) for pirimicarb, phosphamidon, and demeton S-methyl re 49, 31, and 5, respectively. However, for Yesan population to which OPs were sprayed 5 times, RRs for pirimicarb, phosphamidn, and dementon were 830, 536, and 204, respectively. The esterase activities of Wonju and Yesean populations increased by 44.5 and 92.0% compare to that of Kwagju population. R/S a values (S clone Kwangju population; R clone: Iechon opulation) for acetylcholinesterase (AChE) inhibition (${I}_{50}$) by pirimicarb and phosphamidon were 299.2 and 186.0, respectively. Our results indicate that increase of esterase activity and reduction of AChE sensitivity seemed to contribute insecticide resistance of the spiraea aphid.

• The Korean Journal of Mycology
• /
• v.33 no.1
• /
• pp.35-37
• /
• 2005

Anthracnose symptoms on Spiraea prunifolia were observed around the residential area of Dongbumyon, Geoje City, Gyeongnam Province, Korea in 2002 and 2003. Typical symptoms of the disease were dark red spot on the leaves and black spot on the stem. The pathogen formed gray to dark gray colony on potato dextrose agar. Conidia were single celled, color, ovoid or oblong in shape and $6{\sim}12\;{\times}\;3{\sim}5\;{\mu}m$ in size. Seta were dark brown in color, cone-shaped in shape and $23{\sim}42\;{\times}\;2{\sim}4\;{\mu}m$ in size. The optimum temperature for growth of the isolate was about $25{\sim}30^{\circ}C$. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Colletotrichum gloeosporioides. This is the first report on the anthracnose of S. prunifolia caused by C. gloeosporioides in Korea.

• Natural Product Sciences
• /
• v.27 no.1
• /
• pp.36-44
• /
• 2021

The essential oils from the aerial parts (leaves and flowers) of Spiraea hypericifolia L. (Rosaceae), collected in Northern Kazakhstan, were obtained by distillation in two dispersion media (distilled water and 15% NaCl solution). The chemical composition of the essential oils was evaluated by GC-MS for the first time. The yield of the essential oil was 0.04% (in fresh growth conditions) and 0.02% (in dry growth conditions) respectively regardless of which dispersion media (H2O or 15% NaCl solution) was used at the isolation of essential oil. The main compounds were aliphatic hydrocarbons (alkanes) (40.6-53.2%), aldehydes (8.4-17.4%), diterpenoids (9.1-16.7%) and ketones (6.2-8.7%). Content of monoterpenoids was depended on dispersion media (2.2-3.6% where H2O was dispersion media and 8.4-8.5% where 15% NaCl solution was dispersion media). n-Heneicosane (17.4-34.1%) and n-tricosane (14.3-19.5%) were the main constituents of the essential oil of S. hypericifolia. There were many insects from different classes in S. hypericifolia at flowering. Important components such as α-methylene-γ-butyrolactone (0.8-2.8%), benzyl cyanide (0.7-1.1%), β-damascenone (1.2-2.9%), (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (1.8-2.7%), β-ionone (0.5-1.8%) and others were detected in small amounts.

• Journal of Environmental Science International
• /
• v.20 no.11
• /
• pp.1417-1423
• /
• 2011

For evaluating the effect of various organic fertilizer ratios on the Spiraea${\times}$bumalda 'Gold Mound' growth, a container green wall system experiment was conducted in a greenhouse at Konkuk university. The experimental planting grounds were prepared with different organic fertilizer ratios ($A_1L_0$, $A_8L_1$, $A_4L_1$ $A_2L_1$ and $A_1L_1$) and with drought tolerance and an ornamental value Spiraea${\times}$bumalda 'Gold Mound' was planted. The change in soil moisture contents, plant height, number of branches, number of dead leafs, number of leaf, number of shoots, length of node, length of leaf, width of leaf, root-collar caliper, chlorophyll contents and survival rate were investigated from April to Jun 2010. 1. The result of soil moisture contents was analyzed with weight unit in the container green wall system during the dry summer season. The soil moisture contents were significantly enhanced in the container green wall system in increasing order as the amount of fertilizer level increased $A_1L_1$ > $A_2L_1$ > $A_4L_1$ > $A_8L_1$ > $A_1L_0$. 2. Compared to the control treatment (amended soil with 100% + organic fertilizer 0%) application, the highest plant growth was observed in the treatment of $A_2L_1$(amended soil with 67% + organic fertilizer 13%) application. However, the differences between the organic fertilizer ratio treatments of $A_1L_1$, $A_4L_1$, $A_8L_1$, and the $A_1L_0$ organic fertilizer application were mostly not significant. 3. The survival rate increased with the increasing application of organic fertilizer, but in the control treatment (amended soil with 100% + organic fertilizer 0%) application all the plants died. Experimental results from the presented study clearly demonstrated that the organic fertilizer improved the survival rate more than the Spiraea${\times}$bumalda 'Gold Mound' growth at different levels of organic fertilizers. This strain can be utilized as a plant growth application in living wall systems during the dry summer season. Therefore, Spiraea${\times}$bumalda 'Gold Mound' is expected to be a highly valuable shrub for the green wall system if it should be considered in integration with stormwater retention or as a soil conditioner for increasing soil water contents in planting ground.

• Korean Journal of Environment and Ecology
• /
• v.27 no.3
• /
• pp.357-368
• /
• 2013

This study was carried out to classify and describe the vegetation of algific talus in Korea by methods of the ZM school of Phytosociology. A part of slope or a hollow at the foot of talus in which cool air exhausts from underground through gaps of accumulated rock debris is called algific talus. From the sixty three stands obtained, the following two community and their four subcommunity were recognized: A. Quercus variabilis - Cystopteris fragilis com. B. Quercus mongolica - Betula schmidtii com. B-1. Quercus serrata - Urtica angustifolia subcom. B-2. Sorbus commixta - Spiraea chamaedryfolia subcom. B-3. Zabelia biflora - Astilboides tabularis subcom. B-4. Typical subcom. A. Quercus variabilis - Cystopteris fragilis com.. As a result of DCCA ordination, Quercus variabilis - Cystopteris fragilis com. has Quercus variabilis, Ligustrum var. obtusifolium, Zelkova serrata, Viburnum carlesii etc. as differential species, which showed the higher correlation with area of high Warmth index (WI) and precipitation than Quercus mongolica - Betula schmidtii com. has Quercus mongolica, Sorbaria var. sorbifolia, Magnolia sieboldii, Acer pseudosieboldianum, Rosa acicularis etc. as differential species. Sorbus commixta - Spiraea chamaedryfolia subcom. has Spiraea chamaedryfolia, Sorbus commixta, Rosa koreana, Vaccinium var. hirtum, Acer tegmentosum, Dryopteris expansa, Abies holophylla etc. as differential species. and Typical subcom in Quercus mongolica - Betula schmidtii com. were distributed in higher altitude and precipitation area than others. Zabelia biflora - Astilboides tabularis subcom. has Spiraea chinensis, Zabelia biflora, Astilboides tabularis, Gymnocarpium jessoense, Saussurea mongolica, Larix kaempferi etc. as differential species, which was distributed in area with high amounts of sunlight exposure by human activities.

• Korean journal of applied entomology
• /
• v.32 no.3
• /
• pp.259-264
• /
• 1993

The experiment was carried out to investigate Insecticide susceptibility of the spiraea aphid (Aphis citricola) in apple orchards In Korea, using dipping method. Although insecticIde susceptibility vaned with local and seasonal populations, the susceptibility to demeLon S-rnethyl and phosphamidons was different from that Lo the other insecticides. The LCso values were 10 ppm for deltamethrin and chloropyrifos, 103 ~ 629 ppm for demeton S-methy1, acephate, phosphamidon, monocrotophos and vamldothion, 12,200 ppm for EPN, and 1,745 ppm for pirimicarb. 'When Insecticide susceptibility was compared between the S-clone selected from a population on the host plant spiraea and the R-clone selected from a population on the apple tree, the resistance ratio as expressed by RIS ratio of $LC_{50}$ was 78 for phosphamidon and 546 for pirimicarb. Esterase zymogram determined by the agar gel electrophoresIs revealed a significant difference between the clones. The activity of the E2, E5, E6 and E7 of the R-clone was higher than that of the S-clone, It is suggesLed that the Increased esterase activity may be involved in the mechanism of insecticide resistance in the spiraea aphid, although the involvement of other factor(s) may not be ruled out.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.15 no.2
• /
• pp.103-115
• /
• 2012

To investigate the attributes of seed germination of the plants for greening of river bank slope, low-temperature treatment, chemical treatment, plant hormones treatment, and stratification were conducted using the seeds of 6 herbs growing naturally around river banks, such as Setaria glauca and faberii, Imperata cylindrica var. koenigii, Miscanthus sinensis, Pennisetum alopecuroides, Lespedeza cuneata and the seeds of 3 shrubs, such as Lespedeza bicolor., Rosa multiflora, Spiraea prunifolia for. simpliciflora. The findings are as follows. In case of herbs, Setaria glauca and faberii. have stone seeds. In the treatment with $H_2SO_4$, they were germinated through dormancy breaking, even though there were some differences depending on immersion time, and the germination rate of Setaria faberii was a bit higher than that of Setaria glauca. The germination rate of Imperata cylindrica var. koenigii was low in the treatment with $GA_3$, NAA, and the plant hormone, but the seed germination rate has improved and the germination rate of Miscanthus sinensis and Pennisetum alopecuroides showed the improved seed germination rate in low temperature treatment. In case of Lespedeza cuneata and bicolor, the leguminous plants, the treatment with $H_2SO_4$ was most effective for the improvement of seed germination and the decrease in germination time. Only Spiraea prunifolia for. simpliciflora, the rose among 3 woody plants showed the seed germination rate above 50% without any additional treatment. Thus, it was thought to be appropriate for greening. Rosa multiflora showed the attribute of germination in a dark condition at low temperature through stratification, despite its low germination rate. Based on the results as above, with respect to the greening using directly collected seeds, Miscanthus sinensis, Pennisetum alopecuroides, and Spiraea prunifolia for. simpliciflora are suitable for greening, because they do not need any additional treatment, and Lespedeza cuneata and bicolor are also suitable for greening because it is possible to improve the germination rate and reduce the germination time through the treatment on the seeds before sowing.

• Korean Journal of Plant Taxonomy
• /
• v.45 no.4
• /
• pp.332-352
• /
• 2015

The tribe Neillieae, a small group of about 18 species in the Rosaceae, comprises three taxonomically difficult genera, Neillia, Physocarpus, and Stephanandra. The tribe, characterized by lobed leaves with persistent or deciduous stipules and ovoid, shiny seeds with copious endosperm, is strongly supported as a monophyletic group by a variety of lines of molecular evidence. Due to the high amount of morphological variation across the three genera and the species in tribe Neillieae, conflicting classification schemes and numerous species have been proposed over the past three centuries. However, no comprehensive systematic study of the group, including all species across their geographic ranges, has ever been undertaken. As part of a taxonomic revision of tribe Neillieae, a revision of Physocarpus based on the morphological examination of herbarium specimens, including types, and field observation is presented. Artificial keys, comprehensive nomenclatural treatments, descriptions, distribution maps, and lists of specimens examined are provided. Six species in Physocarpus are recognized. A lectotype is here designated for the following species: Opulaster pubescens, Opulaster ramaleyi, Spiraea opulifolia var. parvifolia, Spiraea opulifolia var. tomentella, Physocarpus michiganensis, and Physocarpus missouriensis.