• Title/Summary/Keyword: Speed breeding

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Effect of Priming Treatment Using PEG on Seed Germination in Acer mono Max. (PEG를 이용한 priming 처리가 고로쇠나무 종자 발아에 미치는 영향)

  • Tak, Woo-Sik;Cho, Kyeong-Jin;Choi, Chung-Ho
    • Korean Journal of Plant Resources
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    • v.19 no.4
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    • pp.480-487
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    • 2006
  • This study was conducted to improve the germination percent and germination speed by seed priming using polyethylene glycol (PEG) 8,000 solution on Acer mono seed because it has low and irregular germination characteristics. Seed priming was carried out under 0, -0.25, -0.5, -1.0 and -2.0 MPa of water potential at $20^{\circ}C$ for 3 days. Primed seeds showed higher percent germination, germination speed, mean germination time (MGT) and germination performance index (GPI) than unprimed seeds. Especially, primed seed with -2.0 MPa of water potential had the highest germination properties. And primed seeds had a lower dormancy than the unprimed seeds. Relative growth rate and T/R ratio of seedling from primed seeds were measured to survey the effect of seed priming on the seedling vigor as well as seed germination. The seedlings from primed seeds at -2.0 MPa of water potential showed the highest relative growth rates of height and root collar diameter, however, the lowest value in T/R ratio. This study suggested that seeds priming at -2.0 MPa of water potential under $20^{\circ}C$ for 3 days enhanced not only the percent germination, germination speed, MGT and GPI but also the vigor and growth of seedlings in Acer mono.

Genetic Analysis Strategies for Improving Race Performance of Thoroughbred Racehorse and Jeju Horse (서러브레드 경주마와 제주마의 경주 능력 향상을 위한 유전체 분석 전략)

  • Baek, Kyung-Wan;Gim, Jeong-An;Park, Jung-Jun
    • Journal of Life Science
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    • v.28 no.1
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    • pp.130-139
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    • 2018
  • In ancient times, horse racing was done in ancient European countries in the form of wagon races or mountain races, and wagon racing was adopted as a regular event at the Greek Olympic Games. Thoroughbred horse has been bred since 17th century by intensive selective breeding for its speed, stamina, and racing ability. Then, in the 18th century, horse racing using the Thoroughbred species began to gain popularity among nobles. Since then, horse racing has developed into various forms in various countries and have developed into flat racing, steeplechasing, and harness racing. Thoroughbred racehorse has excellent racing abilities because of powerful selection breeding strategy for 300 years. It is necessary to maintain and maximize horses' ability to race, because horse industries produce enormous economic benefits through breeding, training, and horse racing. Next-generation sequencing (NGS) methods which process large amounts of genomic data have been developed recently. Based on the remarkable development of these genomic analytical techniques, it is now possible to easily carry out animal breeding strategies with superior traits. In order to select breeding racehorse with superior racing traits, the latest genomic analysis techniques have to be introduced. In this paper, we will review the current efforts to improve race performance for racehorses and to examine the research trends of genomic analysis. Finally, we suggest to utilize genomic analysis in Thoroughbred racehorse and Jeju horse, and propose a strategy for selective breeding for Jeju horse, which contributes job creation of Korea.

Development of a Continuous High-Speed Single-Kernel Brown Rice Sorting Machine Based on Rice Protein Content

  • Natsuga, Motoyasu;Nakamura, Akitoshi;Kawano, Sumio
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1616-1616
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    • 2001
  • To select kernels for breeding that have required constituent content from either naturally distributed samples or artificially mutated ones, it is necessary to process batch samples in a short time. The constituent content of single-kernel grains such as wheat and rice has been determined using conventional bench type NIR instruments; however, it takes a lot of time and effort. Shizuoka Seiki (Fukuroi-city, Japan) and NFRI (National Food Research Institute) of MAFF (Ministry of Agriculture, forestry and Fisheries of Japan) have jointly developed a continuous high-speed single-kernel brown rice sorting machine based on rice protein content. It consists of several sections such as a feeding mechanism, measuring unit, sorting mechanism and controlling PC. The feeding mechanism picks up single-kernel brown rice from the hopper (maximum of 5kg storage capacity) and sends it to the measuring unit. A spectrum of the brown rice is obtained in the measuring unit, which consists of a near-infrared array sensor. The brown rice is then sorted in the sorting mechanism based on its protein content estimated by the controlling PC. In the present study, measuring speed was approximately 500ms for the full spectrum range and overall sorting speed was approximately 2.8s for one kernel. Accuracy of estimation was approximately SEP=0.5% of dry matter protein content for nonglutinous rice.

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Development of Algorithm in Analysis of Single Trait Animal Model for Genetic Evaluation of Hanwoo (단형질 개체모형을 이용한 한우 육종가 추정프로그램 개발)

  • Koo, Yangmo;Kim, Jungil;Song, Chieun;Lee, Kihwan;Shin, Jaeyoung;Jang, Hyungi;Choi, Taejeong;Kim, Sidong;Park, Byoungho;Cho, Kwanghyun;Lee, Seungsoo;Choy, Yunho;Kim, Byeongwoo;Lee, Junggyu;Song, Hoon
    • Journal of Animal Science and Technology
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    • v.55 no.5
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    • pp.359-365
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    • 2013
  • Estimate breeding value can be used as single trait animal model was developed directly using the Fortran language program. The program is based on data computed by using the indirect method repeatedly. The program develops a common algorithm and imprves efficiency. Algorithm efficiency was compared between the two programs. Estimated using the solution is easy to farm and brand the service, pedigree data base was associated with the development of an improved system. The existing program that uses the single trait animal model and the comparative analysis of efficiency is weak because the estimation of the solution and the conventional algorithm programmed through regular formulation involve many repetition; therefore, the newly developed algorithm was conducted to improve speed by reducing the repetition. Single trait animal model was used to analyze Gauss-Seidel iteration method, and the aforesaid two algorithms were compared thorough the mixed model equation which is used the most commonly in estimating the current breeding value by applying the procedures such as the preparation of information necessary for modelling, removal of duplicative data, verifying the parent information of based population in the pedigree data, and assigning sequential numbers, etc. The existing conventional algorithm is the method for reading and recording the data by utilizing the successive repetitive sentences, while new algorithm is the method for directly generating the left hand side for estimation based on effect. Two programs were developed to ensure the accurate evaluation. BLUPF90 and MTDFREML were compared using the estimated solution. In relation to the pearson and spearman correlation, the estimated breeding value correlation coefficients were highest among all traits over 99.5%. Depending on the breeding value of the high correlation in Model I and Model II, accurate evaluation can be found. The number of iteration to convergence was 2,568 in Model I and 1,038 in Model II. The speed of solving was 256.008 seconds in Model I and 235.729 seconds in Model II. Model II had a speed of approximately 10% more than Model I. Therefore, it is considered to be much more effective to analyze large data through the improved algorithm than the existing method. If the corresponding program is systemized and utilized for the consulting of farm and industrial services, it would make contribution to the early selection of individual, shorten the generation, and cultivation of superior groups, and help develop the Hanwoo industry further through the improvement of breeding value based enhancement, ultimately paving the way for the country to evolve into an advanced livestock country.

System for Improvement of Soybean Using Gene Editing Technology

  • Ji Hyun Bae;Gyu Tae Park;Soo-kwon Park;Yu-na Kim;Dool-Yi Kim;Hyeon Jung Kang;Jung Kyung Moon;Mi-Suk Seo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.234-234
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    • 2022
  • Gene-editing is currently one of the most popular technologies in recent years. Development of the new crop using the gene editing have advantage of improved accuracy and efficiency compared with conventional breeding. Soybean (Glycine max L.) is one of the most important crops worldwide used as food and forage. We tried to establish a system for breeding improvement of soybean through gene-editing technology. For the gene-editing system of soybean, i) selection of efficiency gRNA of targeted gene, ii) efficient genetic transformation of the selected gRNA, iii) selection of trans-clean mutant is essential. First of all, we investigated the selection conditions of gRNA with high editing efficiency of targeted gene using isolated protoplast of soybean. Furthermore, we performed the Agrobacterium-mediated genetic transformation of various soybean cultivars. We identified the tissue culture ability in 23 soybean cultivars for genetic transformation of soybean. The six cultivars with high tissue culture ability were selected and confirmed the transgenic plants in four cultivars. Finally, we established a speed-breeding system as a powerful tool for the fast selection of trans-clean mutants from transgenic plants. Our laboratory will provide the valuable system for improvement of soybean by the gene-editing technology.

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Comparison of Growth Rate and Cold Tolerance with Basic Species, Commercial Lines, and Breeding Lines of Zoysiagrass (한국잔디류 기본종, 상업종 및 육종계통들의 생육속도 및 내한성 비교)

  • Choi, Joon-Soo;Yang, Geun-Mo
    • Asian Journal of Turfgrass Science
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    • v.19 no.2
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    • pp.131-140
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    • 2005
  • This study was conducted to compare cold tolerances and growth rates of 5 basic species, commercial lines, and breeding lines of zoysiagrass. Total 41 zoysiagrasses were tested at the northern part (Jeokseong-myeon) and the middle part (Cheonan) of South Korea. Cold tolerance (survival rate during winter), growth rate, green-up speed, and density data were collected visually. Four types of zoysiagrasses (Z. matrella, Z. tenuifolia, USm, and S4M2) with genetic characteristics of Z. matrella died during winter cold, while the others survived. Midium leaf type zoysiagrasses, such as Anyang1, Samdeock1, Anyang2, SJ21, and Pyeongdong, with genetic characteristics of both Z. sinica and Z. macrostachya showed vigorous growth rate one year after planting at the northern part (Jeokseong-myeon). Twenty nine ($70.7\%$) zoysiagrasses showed vigorous growth rate, but the other twelve ($29.3\%$) zoysiagrasses died by cold and drought condition at the middle (Cheonan) part of S. Korea. Anyang2, AJ9-7, Samdeock1, Samdeock2, Samdeock3, and Zenith-C showed vigorous growth rate even though cold and drought environmental condition were artificially provided at these region. But, SJ2-19, NSm, 88Mey, 88Mey-7S, 88Mey-9S, SJ2l-10, ASm, and DBm which did not die at the northern part, showed serious injury at the middle part of S. korea. From this results, we can conclude that drought is as important factor as low temperature for zoysiagrasses during winter.

Study on the Suitable Fermentation Period of Berry Sawdust for the Development of Protaetia brevitarsis Larva (흰점박이꽃무지 유충의 발육에 적합한 베리류톱밥 발효기간에 관한 연구)

  • Ju-Rak Lim;Sang-sik Lee;Eun-Jin Lee;Woong Kim;Chang-hak Choi
    • Korean journal of applied entomology
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    • v.62 no.3
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    • pp.183-191
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    • 2023
  • After examining the temperature change in berry sawdust during the fermentation period and the developmental characteristics of Protaetia brevitarsis larvae according to the fermentation period, it was found that the fermentation speed was faster than with oak sawdust and a fermentation period of 50-70 days was the most suitable for larval growth. During the fermentation period, the temperature change in sawdust was quickly fermented as berry sawdust passed a high temperature of more than 60℃ in the early stages of fermentation compared to fermentation speed of oak sawdust, and stabilized within 60 days. In individual breeding, the total growth period of P. brevitarsis larvae by fermentation period was the longest at 104.8 days after fermentation for 30 days, which was a significant difference. There was no difference in the growth period between 50 and 90 days of fermentation. Oak sawdust tended to be the same as berry sawdust, but the larval development period was approximately 30 days. The developmental period by larval stage differed greatly between berries and oak sawdust. The weight of larvae according to the fermentation period of sawdust was the lowest after 90 days of fermentation, with a long fermentation period for both berry and oak sawdust, and there was no difference in the 30-70 days fermentation periods. The survival rate was more than 95% in individual breeding, and there was no difference among the fermentation periods. However, in group breeding, the survival rate was lower than that of individual breeding and was lowest at 90 days of fermentation. In group breeding, the ratio of larvae weighing ≥ 2.5 g, which is the standard for shipment, was 85% after 30-70 days of fermentation of berry sawdust, but was lower at 70% after 90 days of fermentation, which was a significant difference. The survival rate with oak sawdust showed the same trend, which was approximately 10% lower than that with sawdust. In addition, the larvae from the berry sawdust weighed > 2.5 g in 40-60 days, while it took 75-105 days with oak-fermented sawdust.

Migration Pattern of the Oriental Honey Buzzard Pernis ptilorhynchus at Hongdo Island in Korea (전남 홍도를 통과하는 벌매(Pernis ptilorhynchus)의 이동 양상)

  • Choi, Young-Bok;Chae, Hee-Young;Kim, Sung-Hyun
    • Korean Journal of Environment and Ecology
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    • v.23 no.1
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    • pp.50-55
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    • 2009
  • To understand the status of the migrating Oriental Honey Buzzard(Pernis ptilorhynchus) in Korea, we carried out a preliminary study on the number and local movements of the Honey Buzzard from September 2005 to October 2007 at Hongdo Island, Jeonnam Province, Korea. A total of 702 Honey Buzzards in 2005, 404 individuals in 2006, and 659 individuals in 2007 were recorded during the fall migration season, but during the spring migration season only two and eight birds were observed in 2006 and 2007 respectively. The migrating Honey Buzzard showed peak numbers from 20 September to 5 October at the study site. According to observation of arrival time of538 Honey Buzzard in October 2007, a total of 2l6 birds(40.l %) were passing over Hongdo Island between 7:00 AM to 8:00 AM, rising skyward on columns of warm air. This suggests the use of thermal currents and avoidance of nocturnal movement for crossing water. They preferred moderate winds(<5 m/s in speed) from northwest, but the migrating number apparently decreased in case of strong winds (${\geqq}5 m/s$). This suggests that wind speed and direction are important environmental factors for migration of the Oriental Honey Buzzard. At present, it is unclear which breeding population(from north-eastern China or Japan) passes over Hongdo Island. Moreover, it is difficult to prove migration route and to understand migratory behaviors of the Oriental Honey Buzzard in Korea. To prove the breeding sites and routes of the migrants over the study site, we need more detailed and further studies such as satellite tracking researches.

Proliferation of Gladiolus 'Topaz' Callus by Liquid Shaking Culture (액체진탕배양에 의한 글라디올러스 'Topaz' 캘러스의 증식)

  • 최정두;김규원
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.157-161
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    • 1999
  • This study was performed to enhance the proliferation rate of Gladiolus 'Topaz' callus. The callus was induced from the cermet tissue explants on MS solid medium with 10 mg/L 2,4-D. In the case of liquid shaking culture, proliferation of the callus was effective in MS medium with 0.05 mg/L 2,4-D at 2$0^{\circ}C$ under 16 hours daylength and in a 100 mL Erlenmeyer flask containing 20 mL of the liquid medium and at 75 rpm in rotation speed of the horizontal shaking culture. Furthermore the callus was also able to be subcultured in the same liquid medium.

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Research on Development of Side Scan Sonar using multi-beam Sensors (멀티빔 센서를 이용한 사이드 스캔 소나 개발에 관한 연구)

  • 장유신;계중읍;구융서;박승수;김지한;이만형
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.696-699
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    • 2004
  • A side scan sonar system uses the towfish installed sonars, It is an equipment that search images of the bottom surface of the sea in real time. It is a typical equipment that is related to a sea investigation such as a geological survey, seabed communication cable and power line cable placing repair investigation, fish breeding ground investigation, sea purification, relic and mineral investigation, and mine and submarine search. It used to fined objects and investigate on the seabed surface. But, recently, it is used to sea purification and geological survey that require information of the correct surface of the seabed. So, it needs various filtering technique and image processing techniques development to acquire high resolution image. therefore, this research develops a side scan sonar using multi-beam sensors that supply various information with the fast scan speed and correct high resolution that is not a simple underwater investigation equipment.

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