• Title/Summary/Keyword: Spectral Characteristic

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Effect of Molecular Weights of Polyethyleneimine on the Polyplex Formation with Calf Thymus DNA

  • Cho, Tae-Sub;Jungho Han;Kim, Seog K.;Lee, Jae-Cheol
    • Macromolecular Research
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    • v.12 no.3
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    • pp.276-281
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    • 2004
  • In this study we investigated the spectral properties, including electric absorption, circular and linear dichroism (CD and LD), and fluorescence emission, of DNA in a DNA-branched polyethyleneimine (BPEI) polyplex at various polymer molecular weights (M$\_$w/) and BPEI-amine-to-DNA-phosphate ratios (N/P ratios). All BPEIs exhibited a common N/P dependence in their absorption and CD spectra. At N/P ratios < 1.0, we observed some hyperchromism in the absorption spectrum, red-shifts in CD bands, and decreases in LD intensity and fluorescence intensity of intercalated ethidium. At intermediate N/P ratios, complete collapse of all spectra occurred. As the N/P ratio increased further, the polyplex dissolved in water. From its characteristic CD spectrum obtained under these conditions, we conclude that the DNA exists in a B-like form. The fluorescence and LD intensities never recovered even at high N/P ratios- which indicates that the dissolved polyplex possesses positive charges and the DNA in the polyplex is condensed despite its B-form CD spectrum. The N/P range in which the absorption and CD signals collapsed was wider when the BPEIs M$\_$w/ decreased. In the case where the BPEIs M$\_$w/ was 0.8 k, recovery of the absorption and CD spectral properties at a high N/P ratio was never achieved, which suggests that the molecular weight of the polymer plays an important role in its dissolution at a high N/P ratio.

A Study on the Measurement of Spectral Characteristics of Semiconductor Light Sources driven by Very Short Pulse Currents (짧은 펄스로 구동되는 반도체 발광소자의 파장측정에 관한 연구)

  • 김경식;김재창;조호성;홍창희
    • Korean Journal of Optics and Photonics
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    • v.1 no.2
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    • pp.198-203
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    • 1990
  • In this paper, a system has been proposed for the measurement of the spectral characteristic of semiconductor light sources driven by very short pulse currents. This system has been constituted a monochrometer of 600 groovedmm grating and of 275 mm focal length, X-Y recorder, scanning motor which enables the system to get the analog data, and amplifier coupled with peak detector. Especially, peak detector was used to convert the short pulse signal to continuous one. In order to verify the resolution with slit width, several slits were made by the hands. By using this system, the spectra of commercial LEDs, AlGaAdGaAs LD, and InGaAsPIInP BH-LD which were driven with pulse current (duty cy$e = 0.01) were measured. From these measurements, it has been shown that the proposed system has about 1 A1$\AA$ resolution and 10$\mu$W sensitivity.

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HMM-Based Bandwidth Extension Using Baum-Welch Re-Estimation Algorithm (Baum-Welch 학습법을 이용한 HMM 기반 대역폭 확장법)

  • Song, Geun-Bae;Kim, Austin
    • The Journal of the Acoustical Society of Korea
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    • v.26 no.6
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    • pp.259-268
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    • 2007
  • This paper contributes to an improvement of the statistical bandwidth extension(BWE) system based on Hidden Markov Model(HMM). First, the existing HMM training method for BWE, which is suggested originally by Jax, is analyzed in comparison with the general Baum-Welch training method. Next, based on this analysis, a new HMM-based BWE method is suggested which adopts the Baum-Welch re-estimation algorithm instead of the Jax's to train HMM model. Conclusionally speaking, the Baum-Welch re-estimation algorithm is a generalized form of the Jax's training method. It is flexible and adaptive in modeling the statistical characteristic of training data. Therefore, it generates a better model to the training data, which results in an enhanced BWE system. According to experimental results, the new method performs much better than the Jax's BWE systemin all cases. Under the given test conditions, the RMS log spectral distortion(LSD) scores were improved ranged from 0.31dB to 0.8dB, and 0.52dB in average.

A Study on SNR Estimation of Continuous Speech Signal (연속음성신호의 SNR 추정기법에 관한 연구)

  • Song, Young-Hwan;Park, Hyung-Woo;Bae, Myung-Jin
    • The Journal of the Acoustical Society of Korea
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    • v.28 no.4
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    • pp.383-391
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    • 2009
  • In speech signal processing, speech signal corrupted by noise should be enhanced to improve quality. Usually noise estimation methods need flexibility for variable environment. Noise profile is renewed on silence region to avoid effects of speech properties. So we have to preprocess finding voice region before noise estimation. However, if received signal does not have silence region, we cannot apply that method. In this paper, we proposed SNR estimation method for continuous speech signal. The waveform which is stationary region of voiced speech is very correlated by pitch period. So we can estimate the SNR by correlation of near waveform after dividing a frame for each pitch. For unvoiced speech signal, vocal track characteristic is reflected by noise, so we can estimate SNR by using spectral distance between spectrum of received signal and estimated vocal track. Lastly, energy of speech signal is mostly distributed on voiced region, so we can estimate SNR by the ratio of voiced region energy to unvoiced.

Field observation of sediment suspension in the surf zone (쇄파대의 저질부유에 관한 현지관측)

  • Shin, Seung-Ho;Kuriyama, Yoshiaki
    • Journal of Navigation and Port Research
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    • v.27 no.4
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    • pp.455-463
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    • 2003
  • Time series of suspended sediment concentration, surface elevation and velocity were measured and analysed to investigate the role of waves and the predominance of infra-gravity wave component for sediment suspension phenomena in the surf zone. For the investigation in detail, we adopted the cross spectral analysis method between suspended sediment concentration and the characteristic values of wave, and ensemble average analysis method about long-period wave component, which is dominant to sediment suspension in the measurement point. The obtained results are summarized as follows: 1)The relationship between suspended sediment concentration and the characteristic values of wave is stronger for the long-period standing wave components(about 60s and 30s where the nodal point of the first mode and the anti-nodal point of the second mode are located at the measurement point, respectively) than the long wave components(about 100s), which have the most energetic power, 2) and also, it is cleared that suspended sediment concentration is increased in the case of the phase, the velocity components of the first mode long-period standing wave(60sec) were accelerated toward on-shore direction, that is, the water surface in offshore side is higher than on-shore side.

Speech Enhancement Based on Modified IMCRA Using Spectral Minima Tracking with Weighted Subband Selection (서브밴드 가중치를 적용한 스펙트럼 최소값 추적을 이용하는 수정된 IMCRA 기반의 음성 향상 기법)

  • Park, Yun-Sik;Park, Gyu-Seok;Lee, Sang-Min
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.49 no.3
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    • pp.89-97
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    • 2012
  • In this paper, we propose a novel approach to noise power estimation for speech enhancement in noisy environments. The method based on IMCRA (improved minima controlled recursive averaging) which is widely used in speech enhancement utilizes a rough VAD (voice activity detection) algorithm which excludes speech components during speech periods in order to improves the performance of the noise power estimation by reducing the speech distortion caused by the conventional algorithm based on the minimum power spectrum derived from the noisy speech. However, since the VAD algorithm is not sufficient to distinguish speech from noise at non-stationary noise and low SNRs (signal-to-noise ratios), the speech distortion resulted from the minimum tracking during speech periods still remained. In the proposed method, minimum power estimate obtained by IMCRA is modified by SMT (spectral minima tracking) to reduce the speech distortion derived from the bias of the estimated minimum power. In addition, in order to effectively estimate minimum power by considering the distribution characteristic of the speech and noise spectrum, the presented method combines the minimum estimates provided by IMCRA and SMT depending on the weighting factor based on the subband. Performance of the proposed algorithm is evaluated by subjective and objective quality tests under various environments and better results compared with the conventional method are obtained.

Differences of EEG and Sleep Structure in Pediatric Sleep Apnea and Controls (소아 수면무호흡증 환아와 정상 소아에서 수면구조와 뇌파 양상 차이)

  • Ahn, Young-Min;Shin, Hong-Beom;Kim, Eui-Joong
    • Sleep Medicine and Psychophysiology
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    • v.15 no.2
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    • pp.71-76
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    • 2008
  • Introduction: In this study, we compared sleep structure, EEG characteristic of pediatric obstructive sleep apnea (OSA) and normal controls which were matched in sex and age. Methods: Fifteen children (male:female=4:11) who complained snoring and were suspected to have sleep apnea and their age and sex matched normal controls (male:female=5:10) have been done nocturnal polysomnography (NPSG). Sleep parameters, sleep apnea variables and relative spectral components of EEG from NPSG have been compared between both groups. Results: Pediatric OSA group were distinguished from normal controls in terms of apnea index, respiratory disturbance index and nadir of oxyhemoglobulin desaturation. Pediatric OSA group showed increased percent of sleep stage 1, decreased rapid eye movement sleep percent and increased delta power in O1 EEG channel. However other sleep parameters and spectral powers were not different between two groups. Conclusion: In pediatric OSA group, sleep structure parameter disruption may be not prominent as the previous studies for adult OSA group because of including mild OSA data in diagnostic criteria. In addition, EEG changes might not be distinct due to low arousal index compared to adult OSA patients. We can observe general characteristics and particularity of pediatric OSA through this study.

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Stability of Phycocyanin and Spectral Characteristic of Phycobilins from Spirulina platensis (Spirulina platensis가 생산하는 phycobilins의 spectral 특성 및 phycocyanin 색소의 안정성)

  • JOO Dong Sik;CHO Soon Yeong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.5
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    • pp.482-488
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    • 2000
  • The stability of c-phycocyanin and spectral property of phycobilins obtained from Spirulina platensis cultured by helical tubular photobioreactor were determined. The c-phycocyanin with maximal absorption of 622 nm and allophycocyanin with maximal absorption of 652 nm fractions were isolated from phycobilins by Sephadex G-100 gel chromatoBraphy, The yield of partially purified c-phycocyanin was about $1.5{\%}$ to dried biomass. The stability of c-phycocyanin in the range of $pH 4{\~}9$ was high but c-phvcocvanin was unstable over pH 10. The c-phycocyanin was stable at temperatures below $40^{\circ}C$, and at light intensity below 15000 lux. And metal ions were not affect the stability of c-phycocyanin.

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Characteristic of the Regression Lines for EMG Median Frequency Data Based on the Period of Regression Analysis During Fatiguing Isotonic Exercise (등장성 운동 시 회귀분석기간에 따른 근전도 중앙주파수 회귀직선의 특징)

  • Kim, Yu-Mi;Cho, Sang-Hyun;Lee, Young-Hee
    • Physical Therapy Korea
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    • v.8 no.3
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    • pp.63-76
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    • 2001
  • Many studies have shown that the initial median frequency (MDF) and slope correlate with the muscle fiber composition. This study tested the hypothesis that the initial MDF and slope are fixed, regardless of the interval at which data are collected. MDF data using moving fast Fourier transformation of EMG signals, following local fatigue induced by isotonic exercise, were obtained. An inverse FFT was used to eliminate noise, and characteristic decreasing regression lines were obtained. The regression analysis was done in three different periods, the first one third, first half, and full period, looking at variance in the initial MDF, slope, and fatigue index. Data from surface EMG signals during fatiguing isotonic exercise of the biceps brachii and vastus lateralis in 20 normal subjects were collected. The loads tested were 30% and 60% maximum voluntary contraction (MVC) in the biceps brachii and 40% and 80% MVC in the vastus lateralis. The rate was 25 flexions per minute. There were no significant differences in the initial MDF or slope during the early or full periods of the regression, but there was a significant difference in the fatigue index. Therefore, to observe the change in the initial MDF and slope of the MDF regression line during isotonic exercise, this study suggest that only the early interval need to be observed.

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Extended Slip-Weakening Model and Inference of Rupture Velocity (Slip-Weakening 모델의 확장과 단층 파열속도의 추정)

  • Choi, Hang;Yoon, Byung-Ick
    • Journal of the Earthquake Engineering Society of Korea
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    • v.24 no.5
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    • pp.219-232
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    • 2020
  • The slip-weakening model developed by Ohnaka and Yamashita is extended over the breakdown zone by equating the scaling relationships for the breakdown zone and the whole rupture area. For the extension, the study uses the relationship between rupture velocity and radiation efficiency, which was derived in the theory of linear elastic fracture mechanics, and the definition of fmax given in the specific barrier model proposed by Papageorgiou and Aki. The results clearly show that the extended scaling relationship is governed by the ratio of rupture velocity to S wave velocity, and the velocity ratio can be determined by the ratio of characteristic frequencies of a Fourier amplitude spectrum, which are corner frequency, fc, and source-controlled cut-off frequency, fmax, or vice versa. The derived relationship is tested by using the characteristic frequencies extracted from previous studies of more than 130 shallow crustal events (focal depth less than 25 km, MW 3.0~7.5) that occurred in Japan. Under the assumption of a dynamic similarity, the rupture velocity estimated from fmax/fc and the modified integral timescale give quite similar scale-dependence of the rupture area to that given by Kanamori and Anderson. Also, the results for large earthquakes show good agreement to the values from a kinematic inversion in previous studies. The test results also indicate the unavailability of the spectral self-similarity proposed by Aki because of the scale-dependent rupture velocity and the rupture velocity-dependent fmax/fc; however, the results do support the local similarity asserted by Ohnaka. It is also remarkable that the relationship between the rupture velocity and fmax/fc is quite similar to Kolmogorov's hypothesis on a similarity in the theory of isotropic turbulence.