• The Plant Pathology Journal
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• v.16 no.5
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• pp.252-256
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• 2000

The 16S-23S rRNA intergenic spacer regions (ISRs) were sequenced and analyzed to design specific primer for identification of Pectobacterium chrysanthemi. Two types ISRs, large and small ISRs, were identified from three strains (ATCC 11663, KACC 10163 and KACC 10165) of P. chrysanthemi and Pectobacterium carotovorum subsp. carotovorum ATCC 15713.Large ISRs contained transfer RNA-Ile(tRNA$^{Ile}$)and tRNA$^{Ala}$, and small ISRs contained tRNA$^{Glu}$. Size of the small ISRs of P. chrysanthemi ranged on 354-356 bp, while it was 451 bp in small ISR of P. carotovorum subsp. carotovorum ATCC 15713. From hypervariable region of small ISRs, species-specific primer for P. chrysanthemi with 20 bp length (CHPG) was designed from hypervariable region of small ISRs, which was used as forward promer to detect P. chrysanthemi strains with R23-1R produced PCR product of about 260bp size (CHSF) only from P. chrysanthemi strains, not from other Pectobacterium spp. and Erwinia spp. Direct PCR from bacterial cell without extracting DNA successfully amplified a specific fragment, CHSF, from P. chrysanthemi ATCC 11663. The limit of PCR detection was 1${\pm}10^2$ cfu/ml.

• Korean Journal of Weed Science
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• v.15 no.1
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• pp.1-12
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• 1995

These researches have been conducted to obtain the basic information of the effects of paraquat on plant species and to screen the plant species showing specific responses to paraquat. Paraquat resistance related to ecotype and overwintering capacity. Perennial and biennial species showed higher resistance than annual species. In a family, most species showed higher resistance were overwintering species. Lamiaceae, Brassicaceae, and Caryophyllaceae were tolerant to paraquat, whereas Poaceae and Asteraceae were sensitive. Especially Mosla dianthera of Lamiaceae, Hemistepta lyrata and Aster pilosus of Asteraceae, and Paspalum thunbergii of Poaceae showed higher tolerance than others. The response patterns of plant species on germination stage were different to those on vegetative stage. Germination of Amarathus lividus, Arabis glabra, and Bidens frondosa was not inhibited by paraquat. But their seedling growth were highly inhibited.

• Journal of Environmental Science International
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• v.30 no.2
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• pp.119-133
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• 2021

The purpose of this study was to provide basic information for rational management plans for sustainable conservation and utilization by comprehensive analyzing the flora distributed in Yongchu valley, Changwon-si. The flora of Yongchu valley were summarized as 319 taxa including 93 families, 212 genera, 285 species, 3 subspecies, 6 forms and 25 varieties. The endemic plants were 5 taxa such as Chrysosplenium pilosum var. fulvum, Impatiens koreana, Lespedeza maritima, Thalictrum acteifolium and Weigela subsessilis. The rare plants were 11 taxa including I. koreana, Prunus yedoensis and Magnolia kobus. The specific plants by floristic region were 38 taxa including I. koreana, Jeffersonia dubia., M. kobus and P. yedoensis which are class V. The naturalized plants were 11 taxa including Solanum americanum, Aster pilosus, Crassocephalum crepidioides and Cerastium glomeratum and so forth and invasive alien plants among these plants was 1 taxa of A. pilosus. The target plants adaptable to climate change were 8 taxa including Corydalis turtschaninovii of northern plant, I. koreana of endemic plant, and Meliosma myriantha of southern plant.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.3
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• pp.13-33
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• 2014

The purpose of this study is to offer the raw data for establishing the management and restoration plan by objective surveying and analysing the flora distributed in Seoak District, Gyeongju National Park, Korea. The flora summarized as 411 taxa including 92 families, 285 genera, 363 species, 2 subspecies, 41 varieties and 5 forms. The rare plants designated by Korea Forest Service were 2 taxa including Exochorda serratifolia and Potentilla discolor. The Korean endemic plants were 3 taxa including Philadelphus schrenkii, Lespedeza maritima and Weigela subsessilis. The specific plants by floristic region were 16 taxa including Asplenium sarelii, Pyrrosia petiolosa, Vitex negundo var. incisa and so forth. The target plants adaptable to climate change were 2 taxa including Lespedeza maritima and Carpesium macrocephalum, and the plants with approval for delivering oversea were 4 taxa including Exochorda serratifolia, Glycine soja, Lespedeza maritima and Weigela subsessilis. The naturalized plants were 51 taxa including Phytolacca americana, Viola papilionacea, Lamium purpureum and so forth. The invasive alien plants were 3 taxa including Ambrosia artemisiifolia, Aster pilosus and Lactuca scariola. NI(Naturalized Index) was 12.4% of all 411 taxa of surveyed flora in this study and UI(Urbanized Index) was 15.9% of all 321 taxa of naturalized plants in Korea. Potentilla discolor in rare plant, Philadelphus schrenkii in endemic plant and Dictamnus dasycarpus in specific plant were established the conservation plan. Whereas, invasive alien plants such as Ambrosia artemisiifolia, Aster pilosus and Lactuca scariola should be removed as soon as possible. The damaged sites in Seoak District were divided between wildfire area and farmland. To restore a forest fire site, we will have to apply a natural renewal and community planting. In case of farmland, we will have to do ecological planting using native species and constrcut a forest wetland.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.367-374
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• 2014

Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such "orphan" genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection.

• The Plant Pathology Journal
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• v.26 no.3
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• pp.267-270
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• 2010

We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.113-124
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• 2007

The vascular plants of the studied area in Keum River lower stream were listed 238 taxa: 74 families, 179 genera, 211 species, 24 varieties and 3 forms. In this, Woody plant were 60 taxa (25.2%), Herb plants were 178 taxa (74.8%) of all 238 taxa vascular plants. Pteridophyta were 4 families, 4 genera, 4 taxa, Gymnospermae were 2 families, 2 genera, 3 taxa and Angiospermae were 68 families, 173 genera, 231 taxa. Based on the list of Korean endemic plant, 2 taxa were recorded; Populus tomentiglandulosa, Forsythia koreana. Specific plant species by floral region were total 7 taxa; Salix siuzevii in class III, 6 taxa (Ainus hirsute, Quercus variabilis, Mallotus japonicus, Plantago camtschatica, Actinostemma lobatum, Sagittaria aginashi) in class I. The naturalized plants in this site were 14 families, 35 genera, 40 species, 2 varieties, 42 taxa and naturalization rate was 17.6% of all 238 taxa vascular plants. Wild plants disturbing ecosystem like Ambrosia artemisiifolia var. etatior have been increasing. So, it needs continuing control and conservation measures on the plant ecosystem.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.113.2-114
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• 2003

Five pepper-infecting potyviruses, Pepper mottle virus (PepMoV), Chilli veinal mottle virus (CVMV), Pepper veinal mottle virus (PVMV), Pepper severe mosaic virus (PSMV) and Tobacco each virus (TEV), are known filamentous virus and can be infected pepper crops systemically. To understand pathology and genome information of the five viruses on pepper plants, host reactions and sequences were compared to the 5 viruses. Five potyviruses were inoculated onto some typical cultivars of hot peppers and compared their symptoms, and virus accumulations. A set of degenerate primers for potyviruses were applied to 5 viruses and RT-PCR was performed. RT-PCR products containing partial nuclear inclusion b and coat protein (CP) genes were cloned. Then, oligo dT primer and species-specific primer were redesigned to amplify the C-terminal part of CP and 3' noncoding regions of each viruses. Sequences of the viruses were analyzed and compared to serological relationships among the viruses. The data can be useful for screening of potyviruses in pepper plants and pathogen-derived transgenic pepper plant development.

• Journal of Plant Biotechnology
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• v.50
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• pp.45-55
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• 2023

The diploid Solanum cardiophyllum, a wild tuberbearing species from Mexico is one of the relatives to potato, S. tuberosum. It has been identified as a source of resistance to crucial pathogens and insects such as Phytophthora infestans, Potato virus Y, Colorado potato beetle, etc. and is widely used for potato breeding. However, the sexual hybridization between S. cardiophyllum and S. tuberosum is limited due to their incompatibility. Therefore, somatic hybridization can introduce beneficial traits from this wild species into the potato. After somatic hybridization, selecting fusion products using molecular markers is essential. In the current study, the chloroplast genome of S. cardiophyllum was sequenced by next-generation sequencing technology and compared with those of other Solanum species to develop S. cardiophyllum-specific markers. The total length of the S. cardiophyllum chloroplast genome was 155,570 bp and its size, gene content, order and orientation were similar to those of the other Solanum species. Phylogenic analysis with 32 other Solanaceae species revealed that S. cardiophyllum was expectedly grouped with other Solanum species and most closely located with S. bulbocastanum. Through detailed comparisons of the chloroplast genome sequences of eight Solanum species, we identified 13 SNPs specific to S. cardiophyllum. Further, four SNP-specific PCR markers were developed for discriminating S. cardiophyllum from other Solanum species. The results obtained in this study would help to explore the evolutionary aspects of Solanum species and accelerate breeding using S. cardiophyllum.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.4
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• pp.18-29
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• 2010

The vascular plants in ecological forest site on the Gujaebong (Mt.), Hadong were listed 338 taxa; 85 families, 219 genera, 290 species, 1 subspecies, 40 varieties and 7 forms. Divided into 338 taxa; woody plants were 121 taxa (35.8%) and herbaceous plants were 217 taxa (64.2%). Based on the legal protection species, endangered wild plants II were recorded; Aconitum austro-koreense, Corylopsis gotoana var. coreana and rare plants (IUCN) by the Korea Forest Service and Korea National Arboretum 5 taxa were recorded; Botrychium virginianum, Aconitum austro-koreense, Coreanomecon hylomecoides, Corylopsis gotoana var. coreana, Viola albida. Based on the list of Korean endemic plants, 13 taxa were recorded; Cephalotaxus koreana, Thalictrum actaefolium, Vicia nipponica, Indigofera koreana, Stewartia koreana, Weigela subsessilis, etc. Based on the list of approved for delivering overseas of plants, 5 taxa were recorded; Coreanomecon hylomecoides, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha, Carex okamotoi. Specific plant species by floral region were total 21 taxa (6.2% of all 338 taxa of vascular plants); Aconitum austro-koreense, Corylopsis gotoana var. coreana in class V, Cimicifuga heracleifolia, Coreanomecon hylomecoides in class IV, 4 taxa (Stewartia koreana, Phacellanthus tubiflorus, Lonicera subhispida, etc.) in class III, Potentilla dickinsii, Viola orientalis in class II, 11 taxa (Aconitum jaluense, Angelica anomala, Erythronium japonicum, etc.) in class I. The naturalized plants in the surveyed sites were 8 families, 15 genera, 15 taxa (Phytolacca americana, Crassocephalum crepidioides, Lolium perenne, etc.) and naturalization rate was 4.4% of all 338 taxa of vascular plants.