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Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers

  • Received : 2010.07.05
  • Accepted : 2010.08.09
  • Published : 2010.09.01

Abstract

We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens.

Keywords

References

  1. Da Silverra, W. D., Ferreira, A., Lancellotti, M., Barbosa, I. A. G. C. D., Leite, D. S., De Castro, A. F. P. and Brocchi, M. 2002. Clonal relationships among avian Escherichia coli isolates determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Vet. Microbiol. 89:323-328. https://doi.org/10.1016/S0378-1135(02)00256-0
  2. Houf, G. R. K., Imberechts, H., Grijspeerdt, K., De Zutter L. and Heyndrickx, M. 2005. Comparison of five repetitivesequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates. J. Clin. Microbiol. 43:3615-3623. https://doi.org/10.1128/JCM.43.8.3615-3623.2005
  3. Matsuura, T., Shinohara, H., Inoue, Y., Azegami, K., Tsushima, Se., Tsukamoto, T. and Mizuno, A. 2007. Erwinia isolates from the bacterial shoot blight of pear in Japan are closely related to Erwinia pyrifoliae based on phylogenetic analyses of gyrB and rpoD genes. J. Gen. Plant Pathol. 73:53-58. https://doi.org/10.1007/s10327-006-0315-7
  4. Mouwen, D. J. M., Weijtens, M. J. B. M., Capita, R., Alonso-Calleja, C. and Prieto, M. 2005. Discrimination of enterobacteral repetitive intergenic consensus PCR types of Campylobacter coli and Campylobacter jejuni by Fourier transform infrared spectroscopy. Appl. Environ. Microbiol. 71:4318-4324. https://doi.org/10.1128/AEM.71.8.4318-4324.2005
  5. Rhim, S. L., Volksch, B., Gardan, L., Paulin, J. P., Langlotz, C., Kim, W. S. and Geider, K. 1999. Erwinia pyrifoliae, an Erwinia species different from Erwinia amylovora, causes a necrotic disease of Asian pear trees. Plant Pathol. 48:514-520. https://doi.org/10.1046/j.1365-3059.1999.00376.x
  6. Sharples, G. J. and Lloyd, R. G. 1990. ERIC an intergene sequence distributed in family enterobacteriacae, Nucleic Acid Res. 18:6503-6508. https://doi.org/10.1093/nar/18.22.6503
  7. Shrestha, R., Koo, J. H., Park, D. H., Hwang, I., Hur, J. H. and Lim, C. K. 2003. Erwinia pyrifoliae, a causal endemic pathogen of shoot blight of Asian pear tree in Korea. Plant Pathol. J. 19:294-300. https://doi.org/10.5423/PPJ.2003.19.6.294
  8. Shrestha, R., Lee, S. H., Kim, J. E., Wilson, C., Choi, S.-G., Park, D. H., Wang, M. H., Hur, J. H. and Lim, C. K. 2007. Diversity and detection of Korean Erwinia pyrifoliae strains as determined by plasmid profiling, phylogenetic analysis and PCR. Plant Pathol. 56:1023-1031. https://doi.org/10.1111/j.1365-3059.2007.01679.x
  9. Szczuka, E. and Kaznowski, A. 2004. Typing of clinical and enviromental Aeromonas sp. strains by random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR, and enterobacteral repetitive intergenic consensus sequence PCR. J. Clin. Microbiol. 42:220-228. https://doi.org/10.1128/JCM.42.1.220-228.2004
  10. Van der Zwet, T. and Keil, H. L. 1979. Fire blight-A bacterial disease of rosaceous plants (Washington, United States Department of Agriculture Handbook 510).
  11. Versalovic, J., Koeuth, T. and Lupski, J. R. 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19:6823-6831. https://doi.org/10.1093/nar/19.24.6823
  12. Wilson, K. 1989. Preparation of genomic DNA from bacteria. In: F. M. Ausubel, R. Brent, & R. E. Kinston (Eds.), Current Protocols in Molecular Biology, 1:2.4.1-2.4.5) John Wiley & Sons, New York, USA.
  13. Wong, H. C. and Lin, C. H. 2001. Evaluation of typing of Vibrio Parahaemolyticus by three PCR methods using specific primers. J. Clin. Microbiol. 39:4233-4240. https://doi.org/10.1128/JCM.39.12.4233-4240.2001