• Coupled systems mechanics
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• 제2권1호
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• pp.23-51
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• 2013

An analytical algorithm for the estimation of the resistance forces exerted on the dipper of a cable shovel and the specific energy consumed in the cutting-loading process is presented. Forces due to payload and to cutting of geomaterials under given initial conditions, cutting trajectory of the bucket, bucket's design, and geomaterial properties are analytically computed. The excavation process has been modeled by means of a kinematical shovel model, as well as of dynamic payload and cutting resistance models. For the calculation of the cutting forces, a logsandwich passive failure mechanism of the geomaterial is considered, as has been found by considering that a slip surface propagates like a mixed mode crack. Subsequently, the Upper-Bound theorem of Limit Analysis Theory is applied for the approximate calculation of the maximum reacting forces exerted on the dipper of the cable shovel. This algorithm has been implemented into an Excel$^{TM}$ spreadsheet to facilitate user-friendly, "transparent" calculations and built-in data analysis techniques. Its use is demonstrated with a realistic application of a medium-sized shovel. It was found, among others, that the specific energy of cutting exhibits a size effect, such that it decreases as the (-1)-power of the cutting depth for the considered example application.

• ETRI Journal
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• 제33권4호
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• pp.600-610
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• 2011

This paper presents a novel approach to face detection by localizing faces as the goal-specific saliencies in a scene, using the framework of selective visual attention of a human with a particular goal in mind. The proposed approach aims at achieving human-like robustness as well as efficiency in face detection under large scene variations. The key is to establish how the specific knowledge relevant to the goal interacts with the bottom-up process of external visual stimuli for saliency detection. We propose a direct incorporation of the goal-related knowledge into the specification and/or modification of the internal process of a general bottom-up saliency detection framework. More specifically, prior knowledge of the human face, such as its size, skin color, and shape, is directly set to the window size and color signature for computing the center of difference, as well as to modify the importance weight, as a means of transforming into a goal-specific saliency detection. The experimental evaluation shows that the proposed method reaches a detection rate of 93.4% with a false positive rate of 7.1%, indicating the robustness against a wide variation of scale and rotation.

• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.460-464
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• 2013

Single-chain variable fragments of antibodies (scFv) specific to 2,4-dinitrotoluene (DNT) were isolated from a phage library displaying synthetic human scFv fragments with 6 diversified complementary determining regions (CDRs). A DNT derivative that contained an extended amine group was synthesized and conjugated to the NHS-group that was linked to magnetic beads. Phages specific to the immobilized DNT derivatives were isolated from the library after 4 rounds of sequential binding and elution processes. The displayed scFv fragments from the isolated phages showed consensus CDR sequences. One DNT-specific scFv was expressed in E. coli and purified using Ni-affinity chromatography. The purified DNT-specific scFv binds specifically to the immobilized DNT-derivative with $K_D$ value of $6.0{\times}10^{-7}$ M. The scFv and DNT interaction was not disrupted by the addition of 4-nitrotoluene or benzoic acid. These data demonstrate that the screened scFv from the phage displayed library could be used for selective and sensitive detection of explosives such as TNT.

• 한국컴퓨터그래픽스학회논문지
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• 제17권1호
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• pp.17-24
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• 2011

컴퓨터 그래픽스에서 입자 기반의 유체와 강체 모델과의 상호작용을 정확히 시뮬레이션 하는 것은 중요한 문제이다. 일반적으로 이러한 상호작용은 시간에 대해 연속적이지 않은 환경에서 시뮬레이션 되어왔다. 이로 인해 상호작용을 시뮬레이션하는 데 있어서 많은 오차가 있었다. 본 논문에서는 이러한 불연속적인 환경에서 발생하는 오차를 해결하는 방법을 제안한다. 강체 모델의 거리함수장인 음함수가 공간에 따라 연속적으로 증가하는 특성을 이용하여 입자 충돌을 예측하는 예측 기반 충돌 처리 기법을 제안한다. 유체입자와 강체 모델이 충돌할 때 정확한 충돌시점과 충돌 지점을 계산한다. 이를 통하여 유체와 강체가 실제 환경인 연속적인 환경에서와 같이 상호작용하도록 시뮬레이션 하였다.

• BMB Reports
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• 제34권5호
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• pp.446-451
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• 2001

The interaction of cytochrome c with binary phospholipid mixtures was investigated by solid-state $^2H$- and $^{31}P$-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphatidylcholine (PC), and cardioliph (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PC/PG, PE/CL and PE/PG liposomes. In contrast, the splittings of CL decreased on binging of protein to the PC/CL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PC/CL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the $^{31}P$-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PC/CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PC/CL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.

• 한국게임학회 논문지
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• 제16권1호
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• pp.43-50
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• 2016

본 연구의 목적은 특정 게임성 향상을 목표로 제작된 모바일 퍼즐게임을 기존 상용 게임들과 비교해 해당 게임성이 어느 정도의 경쟁력을 가지고 있는지에 대한 평가를 시도하는 것이다. 상용화에 앞서 모바일게임이 목표로 하고 있는 게임성에 대한 평가가 이루어진다면 이후에 진행되는 마케팅, 서비스와 같은 사업절차들이 보다 효율적으로 시행될 수 있을 것이다. 평가 대상 게임은 match 3 방식의 모바일 퍼즐게임이며 캔디크러쉬사가와 포코팡을 비교대상 게임으로 설정하여 비교분석하였다. 평가를 위해 선정한 게임성 평가지표는 몰입감, 도전감, 지속적사용의도이며 각 평가지표에 대한 중요도를 AHP를 통해 산출하였다. 이후 세 가지 게임을 쌍대비교방식으로 평가하고 평가지표의 중요도를 적용하는 방식으로 종합적 게임성에 대한 비교평가를 시도하였다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 추계학술대회 논문집(제1권)
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• pp.181-186
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• 2006

본 논문에서는 Freak Wave의 생성원인에 대하여 간략히 논의하였으며, 이 중 파랑-조류 비선형 상호작용에 대한 이론 및 수치적 해석기법의 역사와 장단점 등을 기술한다. 본 연구에서는 파랑-조류 상호작용에 대한 수치모델링 및 해석 기법을 개발하고 있다. 개발 중인 수치해석 기법은 공간적으로 불균일한 조류와 파랑의 비선형 상호작용을 해석하기 위하여 Navier-Stokes 방정식을 이용하여 유동현상을 모델링하였으며, 이산화를 위하여 스펙트랄요소법(Spectral Element Method; SEM)을 이용하였다. 또한 자유표면의 운동을 효과적으로 기술하기 위하여 ALE(Arbitrary Lagrangian-Eulerian)기법을 사용하였다. 본 연구의 유동 모델과 수치해석기법의 과정과 특성, 그리고 장점 등에 대하여 논의하였으며, 초기적인 수치해석 결과를 제시하였다. 이를 바탕으로 개발된 수치해석기법의 정확성 및 수렴성을 확인할 수 있다.

• 한국프랜차이즈경영연구
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• 제11권2호
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• pp.23-39
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• 2020

Purpose: Social Network Sites(SNS) have been grown up as a public communication channel consumer frequently participate in. Most of food service brands are utilizing social media for advertising recently. As a result of SNS marketing, food service brands anticipate positive outputs from SNS engagement and consumer brand engagement so that we need to verify structural relationship among SNS characteristics, SNS engagement and consumer brand engagement. Research design, data, and methodology: This study identifies that SNS characteristics have effects on SNS engagement and examines relationship between SNS engagement and behavioral engagement. We conceptualize SNS characteristics as information quality, hedonic level and interaction. Furthermore, SNS engagement is composed of SNS participation, positive word of mouth(WOM). In order to verify the purposes of this research, research model and hypotheses were developed. All constructs were measured with multiple items developed and tested in the previous studies. Sample data were collected from 433 online survey panels and analyzed by using SmartPLS 3.2.9. Result: The findings of this research are as follows. First, information quality is positively related with SNS participation. Hedonic level and interaction have impacts on SNS participation and positive WOM respectively. Second, SNS participation has positive effects on positive WOM. Third, both SNS participation and WOM influence behavioral engagement respectively. Conclusions: The implications demonstrate that SNS characteristics such as information quality and hedonic level and interaction exert effects for consumer to participate in SNS brand page. Meanwhile, hedonic level and interaction influence on positive WOM but information quality doesn't. SNS participation and positive WOM affect consumer to engage in specific brand behaviorally as well. Therefore, food service brand marketer is required to manage SNS information quality and hedonic level and interaction among members to encourage SNS participation and positive WOM. As SNS participation and positive WOM increases behavioral engagement of consumer, marketer needs to incentivize SNS participation and look after situation of positive WOM and respond swiftly.

• BMB Reports
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• 제50권8호
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• pp.391-392
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• 2017

Overexpression of mammalian 2-Cys peroxiredoxin (Prx) enzymes is observed in most cancer tissues. Nevertheless, their specific roles in colorectal cancer (CRC) progression has yet to be fully elucidated. Here, a novel molecular mechanism by which PrxII/Tankyrase (TNKS) interaction mediates survival of adenomatous polyposis coli (APC)-mutant CRC cells was explored. In mice with an inactivating APC mutation, a model of spontaneous intestinal tumorigenesis, deletion of PrxII reduced intestinal adenomatous polyposis and thereby increased survival. In APC-mutant human CRC cells, PrxII depletion hindered PARP-dependent Axin1 degradation through TNKS inactivation. $H_2O_2-sensitive$ Cys residues in the zinc-binding domain of TNKS1 was found to be crucial for PARsylation activity. Mechanistically, direct binding of PrxII to ARC4/5 domains of TNKS conferred vital redox protection against oxidative inactivation. As a proof-of-concept experiment, a chemical compound targeting PrxII inhibited the growth of tumors xenografted with APC-mutation-positive CRC cells. Collectively, the results provide evidence revealing a novel redox mechanism for regulating TNKS activity such that physical interaction between PrxII and TNKS promoted survival of APC-mutant colorectal cancer cells by PrxII-dependent antioxidant shielding.

• BMB Reports
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• 제53권2호
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• pp.112-117
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• 2020

A recent study suggested that methylation of ubiquitin-like with PHD and RING finger domain 1 (UHRF1) is regulated by SET7 and lysine-specific histone demethylase 1A (LSD1) and is essential for homologous recombination (HR). The study demonstrated that SET7-mediated methylation of UHRF1 promotes polyubiquitination of proliferating cell nuclear antigen (PCNA), inducing HR. However, studies on mediators that interact with and recruit UHRF1 to damaged lesions are needed to elucidate the mechanism of UHRF1 methylation-induced HR. Here, we identified that poly [ADP-ribose] polymerase 1 (PARP1) interacts with damage-induced methylated UHRF1 specifically and mediates UHRF1 to induce HR progression. Furthermore, cooperation of UHRF1-PARP1 is essential for cell viability, suggesting the importance of the interaction of UHRF1-PARP1 for damage tolerance in response to damage. Our data revealed that PARP1 mediates the HR mechanism, which is regulated by UHRF1 methylation. The data also indicated the significant role of PARP1 as a mediator of UHRF1 methylation-correlated HR pathway.