• The Journal of the Korea Contents Association
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• v.19 no.6
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• pp.1-9
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• 2019

The purpose of this study is to examine the system of the Jeokbyeok-ga's version using the Computer analysis technique of bibliographies and to examine the achievements of the Jeokbyeok-ga's version studies. First, in order to provide basic data for analysis, a raw corpus was constructed for 46 species of Jeokbyeok-ga. Through this, the common narrative units of the Jeokbyeok-ga were identified as 5 layers, and thus 146 individual paragraphs could be extracted. Based on the encoded corpus, we tried to measure the similarity and the distance between the two. Next, we applied the Multidimensional scaling method, Hierarchical cluster analysis and Cladistic analysis method of the system to confirm the distribution of versions group and it was possible to visually grasp the distance between versions and the system of the work. As a result of analyzing Computer analysis technique of bibliographies, it was found that version's group of the Jeokbyeok-ga was divided into a Wanpan(完板) series and Changbon(唱本) series. Also, it was possible to examine the influence relationship between the Pansori's traditions and transmission.

• Journal of Microbiology and Biotechnology
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• v.29 no.8
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• pp.1266-1272
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• 2019

A bacterial strain, labeled $UR11^T$, was isolated from green alga Ulva pertusa collected from Jeju Island, Korea. $UR11^T$ was identified as a gram-negative, rod-shaped, motile by gliding and aerobic bacterial strain with yellow colonies on R2A plates. The strain $UR11^T$ grew over at a temperature range of $10^{\circ}C$ to $30^{\circ}C$ (optimally at $25^{\circ}C$), a pH range of 6.0-11 (optimally at pH 7.0) and a Nacl range of 0.5-5% Nacl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain $UR11^T$ was a member of the genus Flavobacterium. Strain $UR11^T$ shared close similarity with F. jejuensis $EC11^T$ (98.0%) F. jumunjinense $HME7102^T$ (96.1%), F. haoranii $LQY-7^T$ (95.3%), F. dongtanense $LW30^T$ (95.1%), and F. ahnfeltiae 10Alg $130^T$(94.9%). The major fatty acids (>5%) were $iso-C_{15:0}$ (33.9%), $iso-C_{15:1}$ G (12.4%), $iso-C_{17:0}$ 3-OH (9.0%), $isoC_{16:0}$ (7.0%) and $iso-C_{15:0}$ 3-OH (6.3%). The major polar lipids were phosphatidylethanolamine, seven unknown aminolipids, two unknown aminopolarlipids and two unknown lipids. DNA-DNA hybridization value was 58% at strain $UR11^T$ with F. jejuensis $EC11^T$. Based on phenotypic, chemotaxonomic and phylogenetic evidence, strain $UR11^T$ represents a novel species of the genus Flavobacterium, for which the name Flavobacterium jocheonensis sp. nov. is proposed. The type strain is Flavobacterium jocheonensis is $UR11^T$ (=KCTC $52377^T$ =JCM $31512^T$).

• Journal of Microbiology and Biotechnology
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• v.29 no.9
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• pp.1391-1400
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• 2019

Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.

• Journal of the Korean Applied Science and Technology
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• v.36 no.1
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• pp.258-268
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• 2019

The extracts of AS contain in alloxanthin, halocynthiaxanthin, astaxanthin and 13 kinds of carotenoids. The aim of the study was to assess the anti-oxidant activity and cell viability of AS. The anti-oxidant activity was determined by using DPPH radical inhibition activity and superoxide dismutase (SOD)-like activity. The results of cell viability assay showed that the extracts from AS were cytotoxic at concentrations above $5.0mg/m{\ell}$. This study was designed to examine inflammation induced by LPS, protection effect by UVB and the toxicity of Ascidian shell extract(ASE) as a functional cosmetic ingredient. Evaluation of embryo toxicity resulted in embryo coagulation and mortality when treated at 5.0, 10.0, $20.0mg/m{\ell}$. At the lowest concentration of $1.0mg/m{\ell}$, hatchability resulted in 100.0 % rate. The results of arrhythmia measurement in larvae showed similarity to the evaluation of embryo toxicity. This result demonstrated that toxicity is present at concentrations greater than $5.0mg/m{\ell}$. The protective effect of ASE on LPS and UVB-induced in the zebrafish was investigated. Intracellular reactive oxygen species(ROS) generated by the exposure of zebrafish to LPS, UVB-radiation were significantly decreased after treatment with ASE at $0.1mg/m{\ell}$. As a result, ASE similarly reduced UVB-induced ROS generation and cell death in live zebrafsih. Therefore, it is suggested that ASE has anti-Inflammatory effects and can possibly be used as a functional substance for skin protection in the future.

• Fisheries and Aquatic Sciences
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• v.26 no.7
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• pp.437-446
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• 2023

Cyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumption purposes. Among the viruses that infect cyprinids, cyprinid herpesvirus-2 (CyHV-2), koi herpesvirus (KHV, also known as cyprinid herpesvirus-3), and carp edema virus (CEV) are of particular concern as they cause substantial economic losses to the aquaculture industry. In this study, we investigated these viruses in both of domestic and imported cyprinids. Our results revealed that CyHV-2 was only detected in imported goldfish from Thailand. To further investigate the genetic characteristics of them, the marker A region was analyzed. Despite belonging to the same cluster with isolates from China, France, Poland, and Israel, CyHV-2 detected in this study showed distinct differences in their repetitive sequence sizes. Furthermore, two different forms of KHV/CEV coinfection were identified from domestic koi carp, both of which exhibited typical symptoms. Phylogenetic analysis showed that one KHV isolate (ScKc-2105-K) was of the Asian type and closely related to isolates from Japan, Indonesia, Belgium, Taiwan, and China. Two CEV isolates (ScKc-2105-CE and GhKc-2207-CE) be- longed to the IIa type and showed high similarity with isolates from the USA, France, and Korea. Notably, koi carp injected with cultured KHV (ScKc-2105-K) showed 78.0% cumulative mortality within 14 days post-injection (dpi). Our findings support the importance of regular surveillance of viral diseases in cyprinids.

• Korean Journal of Veterinary Research
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• v.64 no.1
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• pp.7.1-7.6
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• 2024

Bird species, particularly poultry and other bird types, including domestic pigeons, are susceptible to fowl pox, a contagious viral disease. The main goal of this study was to validate clinical avipoxvirus diagnoses using molecular analytical methods. The essential components of the investigation were the clinical signs, visible abnormalities, histological changes, and polymerase chain reaction analysis. Twenty out of 120 pigeons had clinical symptoms, which included yellowish crust or nodules near the feet, eyes, and beak. An erosive epidermal lesion and an epidermal acanthotic papular lesion with basal vacuolation were maculopapular evidence associated with significant epidermal hyperkeratosis, as confirmed by histological analysis. In addition, the results showed keratinocyte necrosis beneath the hyperkeratotic epidermal layer, together with superficial and deep dermal perivascular lymphocytic infiltration. In addition, the P4b core protein gene underwent phylogenetic analysis. The sequence analysis results indicated a high degree of similarity across the local strains, with just minor variations observed. Five sample sequences were selected and submitted to the NCBI database. These sequences were identified as OR187728, OR187729, OR187730, OR187731, and OR187732. All the various strains in this research may be classified under clade A of the chicken pox virus phylogenetic classification. This study presents the first description and characterization of pox virus infections in domestic pigeons inside the Basrah governorate.

• Journal of Korean Society of Forest Science
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• v.74 no.1
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• pp.37-46
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• 1986

To study the changes of soil and vegetational composition of Pinus densiflora community by campsite impact of its users, located in Kumo Mountain Provincial Park, the South part of Korea, three sites were selected according to the density of users. The heavy-use sites in this study has changed in the following ways. (1) The concentration of total nitrogen, available phosphate and Ca ion have decreased greatly. (2) From 68 to 73 percent of the undergrowth vegetation has been removed from the $2.000m^2$ camp area, leaving a sparse vegetation cover quite dissimilar in composition to nonuse campsite. (3) The soil hardness was $38.9kg/cm^2$ in heavyuse site and $1.76kg/cm^2$ in nonuse site. (4) The tolerant trees to campsite impact is Acer palmatum, Stephanandra incisa, Rhus trichocarpa, Rubus crataegifolitus and Parthenocissus tricuspidata. (5) Species diversity decreased to a degree at the heavy-use site. Similarity index was 25.38% between the heavy-use and nonuse site.

• Korean Journal of Ichthyology
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• v.13 no.2
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• pp.93-99
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• 2001

Skin mucous cells in the three regions of the body in Pseudobagrus fulvidaraco and Leiocassius nitidus were investigated using three histochemical methods (PAS, AB-PAS and HID). In the two species, components of mucous cell were not distinguished in each region, but presented a differences in position and type, size, and count in all regions. Mucous cells of P. fulvidraco were located in two layers, a superficial epithelial cell and the space within alarm substance cells (ASCs). Mucous cells of L. nitidus were situated only in the superficial epithelial cell layer. The size of mucous cells in the ASC layer were larger than those of the superficial epithelial cells for all measured values: diameter, length, width, and area. In the superficial epithelial cell layer, the average number of mucous cells within the three regions did not differ significantly between species by unpaired T-test, but the average number of those within two cell layers of P. fulvidraco had a similarity at each region by paired T-test. The dismilarity in dorsal and ventral regions in average number of ASCs between P. fulvidraco and L. niditus was confirmed by unpaired T-test.

• Korean Journal of Environment and Ecology
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• v.22 no.4
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• pp.453-460
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• 2008

This study conducted an analysis of plant species composition and vegetation at sea cliffs of the uninhabited islets off Ganghwa-gun by applying discontinuous classification method & continuous ordination method of species composition table by correlation. The analysis results are as follows: First, the plant showing the highest appearance frequency was Parthenocissus tricuspidata Planch.(58.3%), followed by Carpinus coreana Nak., Viburnum carlesii Hemsl. and Kalopanax septemlobus Koidz.(37.5%,respectively). As for a plant community, it was found that it consisted of Quercus mongolica community, Tilia mandshurica community, Carpinus coreana community, Sorbus alnifolia community and Pinus thunbergii community; as for the community distribution classified by individual islet, it was revealed that Quercus mongolica community appeared at Sosongdo and Bunjido; Tilia mandshurica community at Sosongdo and Daesongdo; Carpinus coreana community appeared at Woodo, Suribong and Bido; Sorbus alnifolia community at Susido, Sosongdo and Daesongdo, and Pinus thunbergii community at Seokdo and Woodo, respectively. The plant similarity classified by uninhabited islet was found to be the highest at Sosongdo and Daesongdo. This research judged the reason why the typical grassland community of coastal cliffs did not join these islets to be environmental conditions of rock landform where there exists almost no surface soil at most sea cliffs; thus, more detailed study on this was required.

• Journal of Korean Society of Forest Science
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• v.105 no.3
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• pp.351-359
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• 2016

This study was conducted to analyze the effect of climate change on the tree-ring growth of Pinus koraiensis in Korea. Annual tree-ring growth data of P. koraiensis collected by the $5^{th}$ National Forest Inventory were first organized to analyze yearly growth patterns of the species. When tree-ring growth data were analyzed through cluster analysis based on similarity of climatic conditions, five clusters were identified. Yearly growing degree days and standard precipitation index based on daily mean temperature and precipitation data from 1951 to 2010 were calculated by cluster. Using the information, yearly temperature effect index(TEI) and precipitation effect index(PEI) by cluster were estimated to analyze the effect of climatic conditions on the growth of the species. Tree-ring growth estimation equations by cluster were developed by using the product of yearly TEI and PEI as independent variable. The tree-ring growth estimation equations were applied to the climate change scenarios of RCP 4.5 and RCP 8.5 for predicting the changes in tree-ring growth by cluster of P. koraiensis from 2011 to 2100. The results of this study are expected to provide valuable information necessary for estimating local growth characteristics of P. koraiensis and for predicting changes in tree-ring growth patterns caused by climate change.