[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.12925/jkocs.2019.36.1.258

The Evaluation on the Effectiveness as a Cosmetic Material of Ascidian shell Extract Using Zebrafish model

Park, Sin-Ho (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)
Kim, Bo-Ae (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)
Yang, Jae-Chan (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)

Publication Information

Journal of the Korean Applied Science and Technology / v.36, no.1, 2019 , pp. 258-268 More about this Journal

Abstract

The extracts of AS contain in alloxanthin, halocynthiaxanthin, astaxanthin and 13 kinds of carotenoids. The aim of the study was to assess the anti-oxidant activity and cell viability of AS. The anti-oxidant activity was determined by using DPPH radical inhibition activity and superoxide dismutase (SOD)-like activity. The results of cell viability assay showed that the extracts from AS were cytotoxic at concentrations above $5.0mg/m{\ell}$ . This study was designed to examine inflammation induced by LPS, protection effect by UVB and the toxicity of Ascidian shell extract(ASE) as a functional cosmetic ingredient. Evaluation of embryo toxicity resulted in embryo coagulation and mortality when treated at 5.0, 10.0, $20.0mg/m{\ell}$ . At the lowest concentration of $1.0mg/m{\ell}$ , hatchability resulted in 100.0 % rate. The results of arrhythmia measurement in larvae showed similarity to the evaluation of embryo toxicity. This result demonstrated that toxicity is present at concentrations greater than $5.0mg/m{\ell}$ . The protective effect of ASE on LPS and UVB-induced in the zebrafish was investigated. Intracellular reactive oxygen species(ROS) generated by the exposure of zebrafish to LPS, UVB-radiation were significantly decreased after treatment with ASE at $0.1mg/m{\ell}$ . As a result, ASE similarly reduced UVB-induced ROS generation and cell death in live zebrafsih. Therefore, it is suggested that ASE has anti-Inflammatory effects and can possibly be used as a functional substance for skin protection in the future.

Keywords

Ascidian shell; Zebrafish; Anti-Inflammation; Ultraviolet B; Toxicity; Antioxidant;

Citations & Related Records

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