• Journal of Ginseng Research
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• 제43권1호
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• pp.77-85
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• 2019

Background: Glomus intraradices is a species of arbuscular mycorrhizal fungi that, as an obligate endomycorrhiza, can form mutually beneficial associations with plants. Panax ginseng is a popular traditional Chinese medicine; however, problems associated with ginseng planting, such as pesticide residues, reduce the ginseng quality. Methods: In this experiment, we studied the effect of inoculating G. intraradices on several physiological properties and microbial communities of ginseng. UV-Visible Spectrum method was used to detect physical properties. Denaturing gradient gel electrophoresis method was used to analyze microbial communities. Results: The results indicated that inoculation with G. intraradices can improve the colonization rate of lateral ginseng roots, increase the levels of monomeric and total ginsenosides, and improve root activity as well as polyphenol oxidase and catalase activities. We also studied the bacterial and fungal communities in ginseng rhizospheric soil. In our study, G. intraradices inoculation improved the abundance and Shannon diversity of bacteria, whereas fungi showed a reciprocal effect. Furthermore, we found that G. intraradices inoculation might increase some beneficial bacterial species and decreased pathogenic fungi in rhizospheric soil of ginseng. Conclusion: Our results showed that G. intraradices can benefit ginseng planting which may have some instructive and practical significance for planting ginseng in farmland.

• 식물분류학회지
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• 제50권4호
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• pp.385-394
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• 2020

To elucidate the molecular phylogeny of Neottia japonica, which is a terrestrial orchid distributed in East Asia, the internal transcribed spacer (ITS) of nuclear DNA and the matK of chloroplast DNA were used. A total 22 species of 69 accessions for ITS and 21 species of 114 accessions for matK phylogeny were analyzed with the maximum parsimony and Bayesian methods. In addition, we sought to establish a correlation between the distribution, morphology of the auricles and genetic association of N. japonica with phylogenetic data. The phylogenetic results suggest that N. japonica is monophyletic and a sister to N. suzukii in terms of the ITS phylogeny, while it is paraphyletic with N. suzukii in terms of the matK phylogeny. N. japonica and N. suzukii show similar morphologies of the lip and column, they both flower in April, and they are both distributed sympatrically in Taiwan. Therefore, it appears to be clear that N. japonica and N. suzukii are close taxa within Neottia, although there is incongruence between the nrDNA and cpDNA phylogenies of N. japonica. The incongruence between the two datasets may have various causes, meaning that further studies are needed to confirm the evolutionary process of N. japonica. The phylogenetic status of N. kiusiana, which was not included in previous studies, was as a sister to N. nidus-avis. Meanwhile, the ITS and matK phylogenies are unsuitable for identifying genetic associations with the characteristic of auricles. The phylogenetic topologies of Korean, Taiwanese and mainland Chinese individuals suggest that the populations of N. japonica in Korea originated from China's mainland and island areas. The characterization of regional gene differences could provide useful preliminary data for future studies.

• Genomics & Informatics
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• 제19권3호
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• pp.33.1-33.10
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• 2021

Eucalyptus is one of the major plantation species with wide variety of industrial uses. Polymorphic and informative simple sequence repeats (SSRs) have broad range of applications in genetic analysis. In this study, two individuals of Eucalyptus tereticornis (ET217 and ET86), one individual each from E. camaldulensis (EC17) and E. grandis (EG9) were subjected to whole genome resequencing. Low coverage (10×) genome sequencing was used to find polymorphic SSRs between the individuals. Average number of SSR loci identified was 95,513 and the density of SSRs per Mb was from 157.39 in EG9 to 155.08 in EC17. Among all the SSRs detected, the most abundant repeat motifs were di-nucleotide (59.6%-62.5%), followed by tri- (23.7%-27.2%), tetra- (5.2%-5.6%), penta- (5.0%-5.3%), and hexa-nucleotide (2.7%-2.9%). The predominant SSR motif units were AG/CT and AAG/TTC. Computational genome analysis predicted the SSR length variations between the individuals and identified the gene functions of SSR containing sequences. Selected subset of polymorphic markers was validated in a full-sib family of eucalypts. Additionally, genome-wide characterization of single nucleotide polymorphisms, InDels and transcriptional regulators were carried out. These variations will find their utility in genome-wide association studies as well as understanding of molecular mechanisms involved in key economic traits. The genomic resources generated in this study would provide an impetus to integrate genomics in marker-trait associations and breeding of tropical eucalypts.

• The Korean Journal of Ecology
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• 제27권6호
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• pp.325-333
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• 2004

울산지역의 조류 다양성을 위한 자연경관 구성을 파악하기 위해서 2002년 5월부터 11월까지 센서스를 하였다. 서식지 변수를 1)산림의 형태와 범위, 2)산림이나 농작물로 분류되지 않는 잔류지, 3)경작지 변수의 3가지 주된 범주에 연관시켜 평가하였다. 산림이나 서식지 간의 식생 차이에 대한 군집의 분석은 춘($79.06\%$)${\cdot}$추($57.77\%$)계 모두 산림지역의 군집 구성이 가장 높게 나타났으며, 잔류지는 군집구성($17.98{\sim}23.16\%$)이 거의 비슷하게 나타났고, 경작지는 춘계($2.94\%$)보다는 추계($18.47\%$)에 군집 구성이 보다 높게 나타났다. 196개 지점의 요인 분석에서, 춘계에서 첫 번째 축(component I, $54.8\%$)은 산림지역의 변수인 침염수림과 활엽수림에 강하게 연관되어 있으며, 종과 개체는 첫 번째 축을 따라 좋아하는 활엽수림, 침염수림 및 혼유림으로 분명히 나누어져 있다. 두번째 축(component II, $19.8\%$)은 경작지에 영향을 받는 것으로 나타났다. 추계에서 요인 I($34.8\%$)과 요인II($23.6\%$)는 경작지 주변의 활엽수, 혼유림, 과수원, 저수지, Island에 거의 비슷한 수준으로 연관되어 있으며 종은 좋아하는 소서식지 별로 분명히 나누어져 있다. 53종 1,700여 개체가 기록되었으며, 춘${\cdot}$추계 모두 직박구리, 붉은머리오목눈이, 박새, 참새, 까치 5종이 전체 개체수의 $60\%$ 이상을 차지하였다. 춘${\cdot}$추계 모두 붉은머리오목눈이, 참새, 직박구리, 박새가 중요 종으로 나타났으며, 이들 4개 지역의 종다양성 지수와 종수의 기대치 등을 분석하였는데, 종의 풍부성이란 의미에서 볼 때 춘계에는 원효산($E[S_{59}]=19$)이 가장 높게 나타났으며, 문수산($E[S_{59}]=17$)이 가장 낮게 나타났다. 추계는 거남산($E[S_{63}]=16$)이 가장 높게 나타났으며, 문수산($E[S_{63}]=12$)이 가장 낮게 나타났다. 종의 유${\cdot}$무와 개체수를 비교하여 양 방향 유사도를 분석한 결과 춘계에는 문수산-원효산[0.62]이 비슷한 종과 개체수를 가지고 있었으며, 추계에는 문수산-정족산[0.53]의 조류군집이 매우 유사하게 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1371-1379
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• 2015

Undoubtedly livestock is one of the major contributors to the economy of any country. The economic value of livestock includes meat, dairy products, fiber, fertilizer etc. Understanding and identifying the associations of quantitative trait loci (QTL) with the economically important traits is believed to substantially benefit the livestock industry. The past two decades have seen a flurry of interest in mapping the QTL associated with traits of economic importance on the genome. With the availability of single nucleotide polymorphism chip of various densities it is possible to identify regions, QTL and genes on the genome that explain the association and its effect on the phenotype under consideration. Remarkable advancement has been seen in genome wide association studies (GWAS) since its inception till the present day. In this review we describe the progress and challenges of GWAS in various livestock species.

• 한국작물학회지
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• 제48권
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• pp.41-48
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• 2003

The number of natural products obtained from plants has now reached over 100,000 and new chemical compounds are being discovered ever year. Medicinal and Aromatic plants and their extracts have been used for centuries to relieve pain, aid healing, kill bacteria and insects are important as the antifungal and anti-herbivore agents with further compounds being involved in the symbiotic associations. Although their functions in plants have not been fully established, it is Known that some substances have growth regulatory properties while others are involved in pollination and seed dispersal. The complex nature of these chemicals are usually produced in various types of secretory structures which is an important character of a plant family and also influenced and controlled by genetic and ecological factors. Detailed anatomical description of these structures ave relevant to the market value of the plants, the verification of authenticity of a given species and for the detection of substitution or adulteration. Volatile oils are used for their therapeutic action for flavoring of lemon, in perfumery of rose or as starting materials for the synthesis of other compounds of turpentine. For therapeutic purposes they are administered as inhalations of eucalyptus oil, peppermint oil, as gargles and mouthwashes of thymol and transdermally many essential oils including those of lavender, etc. With these current trend for using volatile components in essential oil will be increasing in the future in Korea and in the world as well.

• 한국환경보건학회지
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• 제35권5호
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• pp.343-354
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• 2009

Since Barker found associations between low birth weight and several chronic diseases later in life, the hypothesis of fetal origins of adult disease (aka, Barker Hypothesis) and epigenetics have been emerging as a new paradigm for geneenvironment interaction of chronic disease. Epigenetics is the study of heritable changes in gene silencing that occur without any change in DNA sequence. Gene expression can be regulated by several epigenetic mechanisms, including DNA methylation and histone modifications, which may be associated with chronic conditions, such as cancers, cardiovascular disease, and type-2 diabetes. One carbon metabolism which involves the transfer of a methyl group catalyzed by DNA methyltransferase is an important mechanism by which DNA methylation occurs in promoter regions and/or repetitive elements of the genome. Environmental factors may induce epigenetic modification through production of reactive oxygen species, alteration of methyltransferase activity, and/or interference with methyl donors. In this review, we introduce recent studies of epigenetic modification and environmental factors, such as heavy metals, environmental hormones, air pollution, diet and psychosocial stress. We also discuss epigenetic perspectives of early life environmental exposure and late life disease occurrence.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.170-176
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• 2012

Genetic diversities, population genetic structures and demographic histories of the thread-sail filefish Stephanolepis cirrhifer were investigated by nucleotide sequencing of 336 base pairs of the mitochondrial DNA (mtDNA) control region in 111 individuals collected from six populations in Korean coastal waters. A total of 70 haplotypes were defined by 58 variable nucleotide sites. The neighbor-joining tree of the 70 haplotypes was shallow and did not provide evidence of geographical associations. Expansion of S. cirrhifer populations began approximate 51,000 to 102,000 years before present, correlating with the period of sea level rise since the late Pleistocene glacial maximum. High levels of haplotype diversities ($0.974{\pm}0.029$ to $1.000{\pm}0.076$) and nucleotide diversities (0.014 to 0.019), and low levels of genetic differentiation among populations inferred from pairwise population FST values (-0.007 to 0.107), support an expansion of the S. cirrhifer population. Hierarchical analysis of molecular variance (AMOVA) revealed weak but significant genetic structures among three groups ($F_{CT}$ = 0.028, p<0.05), and no genetic variation within groups (0.53%; $F_{SC}$ = 0.005, p = 0.23). These results may help establish appropriate fishery management strategies for stocks of S. cirrhifer and related species.

• 한국환경과학회지
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• 제20권6호
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• pp.701-710
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• 2011

The purpose of this study is to investigate the vegetation structure and ecological restoration of disturbed forest due to artificial plant. To this end, 12 plots were set up and surveyed. The result analyzed considering mean importance percentage(M.I.P) showed that the types were divided into three groups which are artificial planted forest type(three plots), natural forest-artificial planted forest type(four plots), natural forest type(five plots). Dominant proportion of artificial planted species were as follows: artificial planted forest type was over 60%, natural forest-artificial planted forest types were 14~49%. The range of Shannon's index of all associations was from 0.7131 to 0.7771(natural forest-artificial planted forest > natural forest > artificial planted forest). Also we suggested restoration method of vegetation for ecological value as follow: Control of density considering step and Remove of Pinus koraiensis seedlings of understory layer and shurb layer.

• Journal of Preventive Medicine and Public Health
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• 제47권5호
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• pp.245-252
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• 2014

Arsenic is a unique element with distinct physical characteristics and toxicity whose importance in public health is well recognized. The toxicity of arsenic varies across its different forms. While the carcinogenicity of arsenic has been confirmed, the mechanisms behind the diseases occurring after acute or chronic exposure to arsenic are not well understood. Inorganic arsenic has been confirmed as a human carcinogen that can induce skin, lung, and bladder cancer. There are also reports of its significant association to liver, prostate, and bladder cancer. Recent studies have also suggested a relationship with diabetes, neurological effects, cardiac disorders, and reproductive organs, but further studies are required to confirm these associations. The majority of research to date has examined cancer incidence after a high exposure to high concentrations of arsenic. However, numerous studies have reported various health effects caused by chronic exposure to low concentrations of arsenic. An assessment of the health effects to arsenic exposure has never been performed in the South Korean population; thus, objective estimates of exposure levels are needed. Data should be collected on the biological exposure level for the total arsenic concentration, and individual arsenic concentration by species. In South Korea, we believe that biological exposure assessment should be the first step, followed by regular health effect assessments.