• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.551-560
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• 2007

We investigated physiological damages and biochemical alleviation of five species of genus Acer under ozone fumigation in order to assess their tolerant ability against ozone toxicity. At the end of 150 ppb $O_3$ fumigation, photosynthetic characteristics were measured, and chlorophyll contents, malondialdehyde (MDA) and antioxidative enzyme activities were analyzed in the leaves of five maple trees (Acer buergerianum, A. ginnala, A. mono, A. palmatum, and A. palmatum var. sanguineum). The reduction of chlorophyll (chl) a in ozone-exposed plants was 16.8% (A. buergerianum) to 26.7% (A. ginnala) of control plants. For the content of chi b, A. ginnala and A. palmatum var. sanguineum represented the high reduction of 26.3% and 23.6%, respectively. The highest reduction on the chi a:b ratio was observed in the leaves of A. palmatum. The reduction of net photosynthesis in five species varied from 2.4% to 37.6%. Among five species, A. ginnala showed remarkable reduction (37.6%) for net photosynthesis in comparison with control. Carboxylation efficiency differed significantly (P < 0.05) among species and between control and ozone treatment. The reduction of carboxylation efficiency was the highest in the leaves of A. ginnala (44.7%). A. palmatum var. sanguineum showed the highest increase (41.7%) for MDA content. The highest increase of superoxide dismutase (SOD) activity represented in A. palmatum (26.1%) and the increase of ascorbate peroxidase (APX) activity ranged from 16.5% (A. ginnala) to 49.1% (A. palmatum var. sanguineum). A. mono showed the highest increase (376.6%) of glutathione reductase (GR) activity under ozone fumigation and A. buergerianum also represented high increase (42.3%) of GR activity. Catalse (CAT) activity increased in the leaves of A. ginnala, A. palmatun and A. palmatum var. sanguineum under ozone exposure, whereas A. buergerianum and A. mono decreased in comparison with control plants. In conclusion, physiological markers such as chlorophyll content and photosynthesis that responded sensitively to $O_3$ in maple trees were considered as the very important indicators in order to evaluate the tolerance against $O_3$ stress, and parameters were closely related with each other. Among anti oxidative enzymes, SOD and APX might be contributed to alleviate to $O_3$ toxicity through the increase of activity in all maple trees. Therefore, these compounds can be used as a biochemical maker to assess the stress tolerance to $O_3$.

• Journal of Korean Society for Atmospheric Environment
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• v.17 no.5
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• pp.395-405
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• 2001

To make an assessment of the compatibility between DOAS and conventional point monitoring system (MCSAM-2: MS2), we investigated the concentrations of three criteria pollutants which include S $O_2$, N $O_2$, and $O_3$from a national monitoring station in Seoul during the periods of June 1999~August 2000. The average concentration values for the whole study period derived from hourly concentration data sets of those three species indicated that the mean differences between the two methods can be approximated as 18%. When the bias structure of two systems was evaluated through the computation of percent difference(PD) between the two such as ( $C_{DOAS}$- $C_{conventional}$ $C_{DOAS}$*100, differences between the two systems appeared to be quite systematic among different compounds. While the mode of bias peaked at 0~20% or 20~40% in terms of PD values, the cause of such positive bias mainly arised from generally enhanced concentration values of DOAS system. The structure of bias among different species was further assessed through linear regression analysis. Results of the analysis indicated that the dominant portions of differences observed from two monitoring systems can be accounted for by the systematic differences in their spanning and zeroing systems. S $O_2$(MS2)=0.6385 S $O_2$(DOAS)+2.0985($r^2$=0.7894) N $O_2$(MS2)=0.6548 N $O_2$(DOAS)+7.437($r^2$=0.7687) $O_3$(MS2)=1.0359 $O_3$(DOAS)-7.7885($r^2$=0.7944) The findings of slope values at around 0.64~0.65 from two species suggest that DOAS should respond more sensitively in upper bound concentration range. The offset values apart from zero indicate that more deliberate comparison needs to be made between these monitoring systems. However, based on the existence of strong correlations from at least 8,000 data points for each species of comparison, we were able to conclude that the compatibility of two monitoring systems is highly significant. With the improvement of calibration techniques for the DOAS system. its applicability for routine monitoring of airborne pollutant species is expected to be quite extendable.

• Bulletin of the Korean Chemical Society
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• v.33 no.11
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• pp.3651-3656
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• 2012

The size-segregated atmospheric aerosols have been collected at 1100 m site of Mt. Halla in Jeju, a background area in Korea, using 8-stage cascade impact air sampler during Asian dust and non-Asian dust storm periods. Their ionic and elemental species were analyzed, in order to examine the pollution characteristics and composition change between Asian dust and non-Asian dust periods. The major ionic species such as nss-$SO_4{^{2-}}$, $NH_4{^+}$, and $K^+$ were predominantly distributed in the fine particles (below $2.1{\mu}m$ diameter), and besides the $NO_3{^-}$ was distributed more in coarse particle fraction than fine particle. On the other hand, the typical soil and marine species i.e., nss-$Ca^{2+}$, $Na^+$, $Cl^-$, and $Mg^{2+}$, were mostly existed in the coarse particles (over $2.1{\mu}m$ diameter). As well in the elemental analysis of aerosols, the major soil-originated Al, Fe, Ca, and others showed prominently high concentrations in the coarse particle fraction, whereas the anthropogenic S and Pb were relatively high in the fine particle fraction. From the comparison of aerosol compositions between Asian dust and non-Asian dust periods, the concentrations of the soil-originated species such as nss-$Ca^{2+}$, Al, Ca, Fe, Ti, Mn, Ba, Sr have increased as 2.7-4.2 times during the Asian dust periods. Meanwhile the concentrations of nss-$SO_4{^{2-}}$ and $NO_3{^-}$ have increased as 1.4 and 2.0 times, and on the contrary $NH_4{^+}$ concentrations have a little bit decreased during the Asian dust periods. Especially the concentrations of both soil-originated ionic and elemental species increased noticeably in the coarse particle mode during the dust storm periods.

• The Korea Journal of Herbology
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• v.27 no.6
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• pp.37-42
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• 2012

Objectives : Angelica Gigantis Radix is prescribed as the root of different Angelica species on the pharmacopoeia in Korea, Japan and China. Chemical components and their biological activities were also different according to their species. A study for the development of simple method to compare Angelica roots was needed. In order to classify them, the methods such as DNA sequencing analysis and taste sensor were applied to three Angelica species like Angelica gigas, Angelica acutiloba and Angelica sinensis. Methods : PCR amplification of intergenic transcribed spacer (ITS) region was performed using ITS1 and ITS4 primer from nine Angelica roots, and then nucleotide sequence was determined. Taste pattern of samples were measured using the taste-sensing system SA402B equipped with a sensing unit, which consists of artificial lipid membrane sensor probes of anionic bitterness, astringency, saltiness, umami, and cationic bitterness (C00, AE1, CT0, AAE, and AN0, respectively). Results : As a result of comparing the similarity of the ITS region sequences, A. sinensis was discriminated from the others (A. gigas and A. acutiloba). Equally this genetic result, A. gigas and A. acutiloba showed similar taste pattern as compared to A. sinensis. Sourness, bitterness, aftertaste of bitterness, astringency, and aftertaste of astringency of A. sinensis were significantly high as compared with A. gigas and A. acutiloba. In contrast, richness was significantly low. Conclusions : These taste pattern can be used as a way of comparison of Angelica species and this technic could be applied to establish a taste pattern marker for standardization of herbs in various purposes.

• The Korea Journal of Herbology
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• v.30 no.3
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• pp.41-47
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• 2015

Objectives : Due to the morphological similarity of the pericarp and description of multi-species in National Pharmacopoeia of Korea and China, the Zanthoxylum Pericarpium is difficult to authenticate adulterant in species levels. Therefore, we introduced the sequence analysis of DNA barcode and identification of single nucleotide polymorphism(SNP) to establish a reliable tool for the distinction of Zanthoxylum Pericarpium from its adulterants. Methods : To analyze DNA barcode region, genomic DNA was extracted from twenty-four specimens of authentic Zanthoxylum species and inauthentic adulterant and the individual internal transcribed spacer regions (rDNA-ITS and ITS2) of nuclear ribosomal RNA gene were amplified using ITS1, ITS2-S2F, and ITS4 primer. For identification of species-specific sequences, a comparative analysis was performed using entire DNA barcode sequences. Results : In comparison of four Zanthoxylum ITS2 sequences, we identified 16, 4, 6, and 4 distinct species-specific nucleotides enough to distinguish Z. schinifolium, Z. bungeanum, Z. piperitum, and Z. simulans, respectively. The sequence differences were available genetic marker to discriminate four species. Futhermore, phylogenetic relationship revealed a clear classification between different Zanthoxylum species showing 4 different clusters. These results indicated that comparative analysis of ITS2 DNA barcode was an useful genetic marker to authenticate Zanthoxylum Pericarpium in species levels. Conclusions : The marker nucleotides, enough to distinguish Z. schinifolium, Z. piperitum, Z. bungeanum, and Z. simulans, were obtained at 30 SNP marker nucleotides from ITS2 sequences. These differences could be used to authenticate official Zanthoxylum Pericarpium from its adulterants as well as discriminating each four species.

• Journal of Mushroom
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• v.11 no.2
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• pp.111-115
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• 2013

This study wascarried out to compare the medicinal effects of various Ganoderma species mycelial extracts. Among 6 Ganoderma species mycelial extracts by using 100% MeOH, G. species ASI-7150 showed the highest antioxidant effect. In nitric oxide (NO) production and ${\beta}$-hexosaminidase release inhibition assay, the treatment of G. lucidum ATCC64251 (Taiwan) mycelia extracts most effectively inhibited NO production in LPS-stimulated RAW264.7 cells and ${\beta}$-hexosaminidase release. In addition, the treatment of all 6 Ganoderma species mycelial extracts were not affect on RAW264.7 cell viability. Although this preliminary research has thrown up many questions in need of further investigation, it will serve as a base for further studies of medicinal effects of various Ganoderma species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.6
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• pp.399-412
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• 2005

This study investigated the composition and the distribution of the benthic polychaeta community in the semi-enclosed Yoja Bay on the southern coast of Korea and was designed to deduce temporal changes in the community with the comparison of the previous studies. In YoJa Bay, benthic polychaetous community structure was investigated on the base of the samples from 87 stations. The surface sediment was mainly composed of fine-grained sediment, mixed clayey silt and silty clay. The total benthic macrofaunal density was $387 indiv./m^2$, and the density was higher in the middle bay, and in the mouth of bay than in the inner bay. scapharca subcrenata (Bivalvia) and amphipods (Crustacea) were important species in the inner and the middle of the bar, respectively. Benthic polychaetes were comprised of 72 species with a mean density of $149 indiv./m^2$. Their distributions showed higher densities in the mouth of bay than in the rest of bay. The dominant species over 1.0 percentages were composed of the 20 species, and they occupied $84.5\%$ of the total density. The most dominant species was Sternaspis scutata ($13.6\%$), fallowed fly Heteromastus filiformis ($9.8\%$), Polynoidae indet. ($8.3\%$), Sigambra tentacuzata ($7.1\%$) and others. The cluster analysis revealed that the study area was divided into three station groups. At the station group YI located in the inner bay, the most dominant species was Heteromastus filiformis . At the station group YII in the middle bay, the dominant species was Sternaspis scutata. And at the station group YIII in the mouth of bay, the dominant species was Paraiacydonia paradoxa. Whereas Sternaspis scutata and Mediomastus sp. were the dominant species in the past study, Heteromastus filiformis and Tharyx sp. appeared newly to be the dominant species, even if their low densities that in the past. These facts mean that Yoja Bay be maintained yet as little polluted area compared to other bays on the coast of Korea, but needed some caution of marine environmental management.

• Korean journal of applied entomology
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• v.54 no.3
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• pp.233-245
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• 2015

The Baekdudaegan Mountain Range is home to a wide range of organisms inhabiting the Korean Peninsula. This study monitored butterfly communities of Guryongryeong (Myeonggye-ri) and Gojingkyeong (Seobyeok-ri) from April to September in 2013 to 2014, and the results were compared with previous data to determine changes in their distribution. We found 1,098 butterflies of 65 different species in Myeonggye-ri, and 1,161 butterflies of 63 different species in Seobyeok-ri. Comparing the two regions, 13 species appeared only in Seobyeok-ri and 15 species appeared only in Myeonggae-ri. When species were ranked according to number of individuals, the top species three species were as follows: Artogeia melete (127, 11.6%), A. napi (100, 9.1%), and Papilio maackii (90, 8.2%) in Myeonggye-ri; and Libythea celtis (146, 12.6%), A. napi (134, 11.5%), and Polygonia c-aureum (69, 5.9%) in Seobyeok-ri. The number of species with fewer than three individuals each was 24 in Myeonggye-ri and 23 in Seobyeok-ri, accounting for a high percentage among the total number of species. The similarities between the two regions were relatively high, and many of the butterflies are on the Red List of Korea. Compared with previous distribution data, there were no significant changes. This consistency indicates that the butterflies of the Baekdudaegan Mountain Range have been conserved well, and therefore the continuity in the Baekdudaegan Mountain Range is necessary to keep in the big picture.

• Korean Journal of Ecology and Environment
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• v.54 no.2
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• pp.96-101
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• 2021

In this study, we wanted to obtain the information of the ecological impact of alien species on native fish communities. The Ichthyofauna and fish community in Joongchosan Reservoir and Bukchosan Reservoir in Gunsan-si Korea were investigated over the period from March to October 2020. During the survey period, a total of 1,895 individuals representing eight species, five families, and four orders were caught in the Joongchosan Reservoir. In the Bukchosan Reservoir, a total of 171 individuals were caught, representing seven species, five families, and three orders. The dominant species and subdominant species of Joongchosan Reservoir were Pseudorasvora parva (661 individuals, RA: 34.7%), and Rhodeus ocellatus (660 individuals, RA: 34.7%). Conversely, Micropterus salmoides (77 individuals, RA: 45.0%), and Carassius auratus (60 individuals, RA: 35.1%), were the two most common species in the Bukchosan Reservoir. The community indices showed a dominance of 0.697, diversity of 1.483, evenness of 0.713, and species richness of 0.928 in the Joongchosan Reservoir. In comparison, the community indices in the Bukchosan Reservoir showed a dominance of 0.801, diversity of 1.304, evenness of 0.670, and species richness of 1.167. This study confirms that the number of native fishes and the associated community diversity have decreased due to the influence of Micropterus salmoides. This highlights the need for removal of alien species from reservoirs, while simultaneously preventing future introductions.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.714-723
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• 2020

This study was conducted to investigate the community stability and similarity of benthic macroinvertebrates in the Giran stream between August and September 2018, and compare results to those reported by Lee (2004). As relates to the total number of species in each taxon in 2018, 45 species were additionally discovered compared to the 2003 study; the number of EPT taxa increased by 14 species and OCH taxa increased by 18 species. The diversity and richness indexes increased while the dominance index tended to decrease. According to analysis of functional feeding groups, 11 more Gathering-collector species were found, making it the highest functional feeding group with 24 species. According to analysis of functional habitat groups, 15 more clinger species were found than in the past, making it the highest functional habitat group with 41 species. A community stability comparison showed that species belonging to 'Stability Group I' had the highest stability rate at 57.1% in 2003 and 61.5% in 2018. According to the biological water quality assessment, in 2018, the average water quality level at each survey site was 'Ia' and 'Very Good' in terms of environmental conditions. As a result of the similarity analysis between the survey points for the species that appeared, two large groups of similarities were classified (similarity group 1: 2003 sites, similarity group 2: 2018 sites).