• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.3
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• pp.258-261
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• 2006

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

• Steel and Composite Structures
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• v.7 no.1
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• pp.71-85
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• 2007

This study suggests modular composite profile beams, where the prefab concept is applied to existing composite profile beams. The prefab concept produces a beam of desired size having two types of profile: side module and bottom module. Module section will improve construction efforts because it offers several benefits : reduction of deflections due to creep and shrinkage, which might be found in existing composite profile beams; increase in span/depth ratio; and free prefabrication of any required beams. Based on the established analysis theory of composite profile beams, an analysis theory of modular composite profile beams was suggested, and analysis values were compared with experimental ones. The behavior of individual modules with increase of load was measured with a strain gauge, and the shear connection ratio between modules was analyzed by using the measured values. As a result of experiment, it was found that theoretical flexural strength on condition of full connection was 57%-80% by connection of modules for each specimen, and it is expected that flexural strength will approximate the theoretical levels through further module improvement.

• Journal of the Korean Ceramic Society
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• v.25 no.3
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• pp.284-292
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• 1988

Alumina-zirconia composite powders for the purpose of improving fracture toughness and thermal shock resistance of alumina were prepared by the emulsion-kerosene drying method. The average particle size of composite powders was less then 1 $\mu\textrm{m}$ and their shapes were spherical. It was shown that the average particle size of composite powders decreased with the concentration of metal-salt in solution and the amount of span 80 added when preparing emulsions. The structure of all zirconia in composite powders heat-treated at 1200$^{\circ}C$ was a tetragonal form at room temperature. This result implied that fine zirconia particles were homogeneously dispersed in the alumina matrix.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.07a
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• pp.315-318
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• 2002

우리나라 원예시설면적은 '00년 말 현재 52,189ha이며, 이중 단동형 비닐하우스 면적은 채소가 34,173ha로 대부분을 차지하고 있다. 단동형 비닐하우스에 사용하는 골재는 주로 ø25mm를 95%이상 사용하고 있으며, 서까래 간격은 지역별로 다르나 대부분 80cm 이상으로 설치되어 있다. 이와 같은 골재와 서까래간격은 구조적으로 매우 취약하여 자연재해에 대비하기 위해서는 자연재해시 사전ㆍ사후대책이 필수적이며, 근본적으로는 비닐하우스 구조적 안전성이 확보되어져야 한다. (중략)

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1997.10a
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• pp.95-102
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• 1997

Jointless bridge is a new construction method applicable to bridge of short length. In the jointless bridge expansion of superstructure due to thermal effect was absorbed in the flexible pile-type abutment in stead of expansion joint in the conventional bridges. By removing expansion joint, it retards deterioration and extends life time of bridge. In this paper, jointless bridge of steel box girder type was studied through finite element analysis. Stress variations of superstructure and pile due to thermal effect was studied for the two span continuous integral bridge of 80m length and the results of analysis was presented.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.05b
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• pp.77-80
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• 2005

Lightweight concrete is known for its advantage of reducing the self-weight of the structures, reducing the areas of sectional members as well as making the construction convenient. Thus the construction cost can be saved when applied to structures such as long-span bridge and high rise buildings. However, the lightweight concrete requires specific mix design method that is quite different from the typical concrete, since using the typical mix method would give rise the material segregation as well as lower the strength by the reduced weight of the aggregate. In order to avoid such problems, it is recommended to apply the mix design method of self-compacting concrete for the lightweight concrete. Experimental tests were performed as such compressive strength, dry shrinkage and carbonation of high strength lightweight self-compacting concrete.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.10 no.16
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• pp.75-80
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• 1987

This paper is deals with the preventive replacement for the equipment which fails only when the total amount of damage reaches a prespecified failure level. Most of replacement model use time as their decision variable, but it is not appropriate for the cases in which failures dependent on their cumulative damage levels. In this paper, a new type preventive replacement model is introduced in which an equipment is replaced before failure when the cumulative damage reaches a certain level or replaced on failure, whichever occures first. The optimal replacement damage levels which minimize total expected cost are obtained by the Dynamic programming Method when the number of use of the equipment is finite. A numerical example is also presented. The optimal preventive replacement policy when the equipment will be used for a finite time span is also discussed.

• Journal of Pharmaceutical Investigation
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• v.21 no.4
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• pp.253-262
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• 1991

Microencapsulation of sodium ascorbate with cellulose acetate phthalate(CAP) by coacervation/ phase separation method were carried out. Various factors affecting microencapsulation, i.e., surfactant concentration. CAP concentration, stirring speed and treatment of spermaceti as a sealing agent were studied. Dissolution rate. particle size distribution, surface feature and stability test were investigated. CAP microcapsules prepared using 0.5% span 80 as a surfactant showed smooth and round surfaces. The release of sodium ascorbate was retarded by microencapsulation with CAP and by sealant treatment with spermaceti. When triturated with sodium bicarbonate, CAP microcapsules were more stable than unencapsulated sodium ascorbate under various RH conditions at $37^{\circ}C$.

• Journal of the Optical Society of Korea
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• v.12 no.4
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• pp.226-231
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• 2008

40 Gbps WDM Systems using super-Gaussian RZ pulses have been studied by numerical simulation to optimize their performance. The assumption of standard single mode fiber is valid when existing WDM systems are required to upgrade their performance to 40Gbps. It is shown that the standard single mode fiber can transmit optical signals over 720 km (Q > 10) by optimizing optical and electrical filter characteristics at the receiver and by compensation of dispersion. However, it is also shown that ${\pm}0.3%$ dispersion compensation tolerance per span (80 km) could prohibit transmitting over 320km (Q > 10). In addition, a duty cycle of less than 0.4 degrades system performance significantly.

• Restorative Dentistry and Endodontics
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• v.27 no.6
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• pp.600-611
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• 2002

본 연구의 목적은 클로로필린이 치주인대 세포 생활력에 미치는 영향을 평가하는데 있다. 치주인대 세포는 건강한 사람에서 발치한 소구치의 치주인대 조직으로부터 채취하여 배양하였다. 비교 기준을 설정하기 위하여, HBSS 내에서 $37^{\ circ}C$로 보관한 치주인대 세포수의 50%가 생존하는데 소요되는 시간을 MTT 분석법에 의해 측정하였다. 그 결과는 6시간이었다. HBSS에 클로로필린 10, 100, 500 nM이 각각 첨가된 3군의 실험 보존액과 F-medium, ViaSpan, Likorol 등 모두 6군의 실험 보존액을 96-well plate에 접종한 후, 치주인대 세포를 동일한 수로 분주하였다. 이를 6시간동안 보관한 후, 각 실험군 보존액에서의 세포 생활력을 MTT 분석법으로 측정하였다 또한, HBSS와 F-medium 및 클로로필린 500 nM이 첨가된 HBSS군의 세포들에 대해 유식세포 계측을 시행하여 각각의 세포주기를 분석하였다. 실험 결과, 클로로필린 500 nM이 첨가된 HBSS에서 보관한 세포들이 가장 높은 생활력을 나타내었으며, 클로로필린이 첨가되지 않은 HBSS에 비해서 유의하게 양호한 세포 생활력 유지 능력을 보였다. 클로로필린으로 처치한 세포들은 클로로필린의 농도가 커짐에 따라 세포 생활력이 증가하는 양상을 나타내었다. 유식세포 계측 결과, HBSS와 F-medium 및 클로로필린 500 nM이 첨가된 HBSS에서 보관한 세포의 77~80%가 G0-G1 단계의 세포 주기로 측정되어, 대부분의 세포들이 안정된 세포 대사 상태를 보이는 것으로 나타났다. 결론적으로, 클로로필린 처치는 치주인대 세포의 생활력 유지에 도움이 되는 것으로 사료된다.