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Present Situation of Diseases Occurred with Cultured Marine Fishes in Kamak Bay (가막만 가두리 양식자의 어류질병에 관한 연구)

  • 최상덕
    • Journal of Aquaculture
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    • v.10 no.1
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    • pp.9-15
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    • 1997
  • The pathogenic organisms occurred in cultured marine fishes in Kamak Bay were investigated from March to November in 1993. The samples were collected at 7 sampling stations once a month. Nine species of pathogenic organisms (Vibrio sp., Edwardsiella sp., Flexibacter sp., Streptococcus sp., Micrococcus sp., Caligus sp., Trichodina sp., Lymphocystis and Staphylococcus sp.) were identified as pathogenic organisms from four different species of fish (Sebastes schlegeli, Paralichthys olivaceus, lateolabrax japonicus and Pagrus major) collected in the study areas. Most of pathogenic organisms were found at over 20^{\circ}C$ of sea water temperature from June to October in 1993. On the test of drug sensitivity, Vibrio sp. (KS-9303) was sensitive to oxytetracycline and chloramphenicol ; Edwardsiella sp. (KP-9315) to oxytetracycline ; Flexibacter sp. (KP-9318) to oxytetracycline, chloramphenicol and oxolinic acid ; Streptococcus sp. (KP-9319) to erythromycin, chlorampheicol and oxytetracycline. However, all these 4 isolated bacteria were resistant to ampicilin, steptomycin, sulfamethoxazole and nitrofurazone.

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Agronomic Variation in Anther Derived Plants of Sweet Pepper (Capsicum annuum L.) Genotypes

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek-Jong;Kang, Won-Hee
    • Korean Journal of Plant Resources
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    • v.23 no.6
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    • pp.503-512
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    • 2010
  • Anther derived double haploids (DHs) from sweet pepper genotypes ('Special', 'Derby', 'Bossanova', 'Fiesta', 'Debora' and 'Minipaprika') were used to study the agronomic variation in 2006. Ninety-nine successful DHs regenerants (32 from 'Special', 25 from 'Derby', 23 from 'Bossanova', 10 from 'Fiesta', 6 from 'Debora' and 3 from 'Minipaprika') were transplanted at plastic house and studied on their agronomic characters. Variation in agronomic characters was observed within the DHs of each genotype. DHs obtained from 'Derby' and 'Fiesta' exhibited wide variation in fruit yield $plant^{-1}$ whereas averaged fruit yield $plant^{-1}$ was highest in 'Derby' (1608 g) and less variation was observed in DHs of 'Bossanova'. Based on the agronomic characters expressed in DHs population at this environment, SP55, SP56, SP60, and SP116 from 'Special', SP8, SP10, SP14, SP16, and SP34 from 'Derby', SP115, SP119, SP142, SP143, SP196, and SP199 from 'Bossanova', SP41, SP45, and SP114 from 'Fiesta', SP21 from 'Debora' and SP91 from 'Minipaprika' identified as elite inbred lines and these DH lines could be used for commercial hybrids production in sweet pepper. Genetic relationship among the selected inbred lines using molecular markers and their response to diseases are further recommended to study.

Natural parasitism of parasitoids on Phyllocnistis citrella (Lepidoptera: Gracillaridae) in Jeju citrus orchards in Korea (제주 감귤원에서 귤굴나방에 대한 포식 기생자 천적의 자연 기생율)

  • Hyun, Seung Young;Lim, Un Taek;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.58 no.4
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    • pp.299-303
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    • 2019
  • This study was conducted to investigate the species of native parasitoids attacking Phyllocnistis citrella and their parasitism in citrus orchards in Jeju, Korea. During the survey period from 2013 to 2014, seven parasitoids belonging to Chalcidoidea were found: majority of the parasitoids were Sympiesis striatipes with 73% and Quadrastichus sp. with 22% in Eulophidae. The others were Neochrysocharis sp., Pnigalio sp., Holcopelte sp., Ageniaspis sp. (Encyrtidae) and Trichomalopsis sp. (Pteromalidae). Average parasitism rate reached 10.7% in orchards with spraying insecticide, and 32.2% in environmental-friendly managed orchards.

Ten new species of families Suberitidae and Polymastiidae (Demospongia: Heteroscleromorpha) from Korea

  • Kim, Young A;Sim, Chung Ja
    • Journal of Species Research
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    • v.10 no.2
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    • pp.168-183
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    • 2021
  • Ten new species of two families (Suberitidae and Polymastidae) are described from Korea. Six new species in Suberitidae: Plicatellopsis ramosa n. sp., Pseudosuberites anheungensis n. sp. P. youngilensis n. sp., Suberites hwasunensis n. sp., S. chujaensis n. sp. and S. rugosa n. sp. are compared with other similar reported species. All new species differ from previously reported species in morphology, texture, color, skeletal structure, and spicule type. Four new species in Polymastidae: Polymastia fistula n. sp., P. sasuensis n. sp., P. viridis n. sp., and Weberella haengwonensis n. sp. are different from previously reported species in the growth form of papillaes and fistules, color and spicule types. For example, Weberella haengwonensis is similar to Weberella verrucosa in shape, but differs based on surface papillaes and spicule sizes.

Interleukin-8 and MCP(Monocyte Chemoattractant Protein)-1 expression by the Human Dental Pulps in cultures stimulated with Substance P (사람치수에서 Interleukin-8과 Monocyte chernoattractant protein-1의 분비에 대한 Substance P의 효과에 관한 연구)

  • Shin, Han-Ju;Park, Sang-Hyuk;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.30 no.3
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    • pp.193-203
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    • 2005
  • The induction of the IL-8 and MCP-1 by the stimulation of Substance P and TNF-${\alpha}$ (IL-8 agonist) and the specificity for SP using Spantide (SP antagonist) in the dental pulp tissues was measured quantitatively. In addition, the secretion of the IL-8 in the human dental pulp tissue 36 hrs after the stimulation of SP was observed after the stimulation of SP qualitatively. According to this study the results were as follows: 1. There was the significant IL-8 induction at 36 h after SP (10$^{-4}$M) stimulation of the pulp tissue comparing with the unstimulated dental pulp tissues (p < 0.05) . IL-8 irnmunostaining was weakly detected along the periphery of the pulp tissue after Mock stimulation and IL-8 immunostaining was detected around the fibroblast in the pulp tissue 36h After SP (10$^{-4}$M) stimulation, 2. The secretion of MCP-1 from the dental pulp tissues comparing with Mock stimulation was induced at 36 hrs after TNF-$\alpha$ (40 ng/ml) stimulation, but no induction with SP(10$^{-4}$M) TNF-${\alpha}$ (40 ng/ml) did not induce the IL-8 secretion from the pulp tissue, weak IL-8 imrnunostaining was detected along the periphery of the pulp tissue 3. Spantide (10$^{-5}$M) inhibited IL-8 induction from the pulp tissues 36 h after SP (10$^{-4}$M) stimulation These results suggest that SP significantly induces IL-8 recruiting neutrophils in localized human dental pulp tissue MCP-1 appears to be less involved in the early establishment of pulpal inflammation in response to irritation such as mechanical insult of dentin. SP may have positive relation with the inflammation of the human dental pulp tissues.

Synthesis and Characterization of Interfacial Properties of Sorbitan Laurate Surfactant (Sorbitan Laurate 계면활성제 합성 및 계면 특성에 관한 연구)

  • Lee, Seul;Kim, ByeongJo;Lee, JongGi;Lim, JongChoo
    • Applied Chemistry for Engineering
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    • v.22 no.1
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    • pp.37-44
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    • 2011
  • The critical micelle concentration (CMC) of sorbitan laurate SP 20 surfactant in this paper was near $7.216{\times}10^{-4}mol/L$ and the surface tension at CMC was about 26.0 mN/m, which showed higher CMC and lower surface tension than those of octylphenol ethoxylate octylphenol ethoxylate (OPE) 10 surfactant. Dynamic surface tension measurement using a maximum bubble pressure tensiometer showed that the adsorption rate at the interface between air and surfactant solution was found to be slower with SP 20 surfactant, presumably due to a low mobility of SP 20 surfactant monomer. The contact angle of SP 20 surfactant solution was observed to decrease with an increase in surfactant concentration and showed a larger value than that of OPE 10 surfactant solution. Half-life time for foams generated with 1 wt% surfactant solution was also larger with SP 20 surfactant, which indicated higher foam stability with SP 20 surfactant. Dynamic behavior study reveals that the solubilization of n-decane oil was much lower with SP 20, which is in good agreement with experimental results of foam stability, contact angle and CMC. Dynamic interfacial tension measurement by a spinning drop tensiometer shows that interfacial tensions at equilibrium condition in both systems were almost the same but the time required to reach equilibrium was longer with SP 20.

Drug Resistance Profiles of Salmonella sp. and Shigella sp. Isolated from Diarrheal Patients in Pusan, Korea (부산지역의 설사환자로부터 분리한 Salmonella sp. 및 Shigella sp.의 약제내성 유형에 관한 연구)

  • 차인호;김용환;빈재훈;김경숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.6
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    • pp.927-932
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    • 1994
  • A total of 34 Salmonella sp. an d25 Shigella sp. were isolated from 311 patients with diarrhea. The isolation rates of Salmonella sp. ad Shigella sp. were 10.9% and 8%, respectively. The serogroups of 34 Salmonella sp. were in order of group D(50%), group B(38.25), group E(8.8%) and group C 92, 9%0. the serogroups of 25 Shigella sp. were group D(96%) and group B(4%). Seasonal distribution of isolated Salmonella sp. and Shigella sp. were shown the most high at July, 17.65% and 64%, respectively. Age group distribution of isolated Salmonella sp. and Shigella sp. were shown the most high at twenties and thirties (23.5%), and teens(76%), respectively. The Salmonella isolates were resistant in order of prevalence use of streptomycin(SM) (100%), erythromycin (EM) and movobiocin (NB)(90.6%), penicillin G(PG) (65.6%) and cephalexin (CPX)(46.9%). the isolates of Shigella sp. were resistant in order of prevalence use of EM (95.8%), NB(91.7%), SM(87.5%). Eighteen kinds of resistant patterns of Salmonella ioslates were detected. The multiple resistance patterns of Shigella isolates were mostly SM, EM, NB type (79.2%). The minimum inhibitory concentration of Salmonella sp. and Shigella sp. and Shigella sp. isolated from patients with diarrhea were tabulated.

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Variations of Species Composition of Early Feeding Organisms Through Nature Mixed Culture (자연혼합배양에 의한 초기 먹이생물의 종 조성 변화)

  • Jung, Min-Min;Oh, Bong-Sae;Ku, Hag-Dong;Lee, Chang-Hoon;Yang, Moon-Ho;Moon, Tae-Seok;Kim, Sam-Yeon;Kim, Hyeung-Sin
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.15 no.1
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    • pp.1-7
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    • 2010
  • We were successfully reared young marine ornamental larva fish in a unique process of microalgae blooming culture tank. The marine fish larva was grown and survived in this method. Generally, we called this method as natural mixed culture. Observed planktonic microalgae were 34 species with 19 diatoms (Detonula pumila, Nitzschia sp., Fragilaria oceanica, Chaetoceros curvisetus, Stephanodiscus sp., Chaetoceros decipies, Chaetoceros sp., Thalassiosira rotula, Eucampia zodiacus, Diploneis splendica, Nitzschia longissima, Surirella cuneata, Asterionella glacialis, Nitzschia spp., Chaetoceros debile, Thalassionema nitzschioides, Nitzschia closterium, Skeletonema costatum and Licmophora sp.), 14 flagellates (Euglena, sp., Gonyaulax sp., Pyramimonas sp., Protoperidinium sp., Eutreptia sp., Parapedinella sp., unidentified micrc-flagellate, Gyrodinium sp., Scrippsiell trochoidea, Gymnodinium sanguineum, Chrysochromulina sp., Gymnodinium sp., Prorocentrum triestinum and Micromonas sp.) and 1 ciliate (Mesodinium rubrum) in this culture tank. Dominant microalgae were Chrysochromulina sp. during the larval rearing periods. Blooming condition maintained continuously and stably from 10 to 60 days in this microcosm.

Anatomy of Spleen Meridian Muscle in human (족태음비경근(足太陰脾經筋)의 해부학적(解剖學的) 고찰(考察))

  • Park Kyoung-Sik
    • Korean Journal of Acupuncture
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    • v.20 no.4
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    • pp.65-75
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    • 2003
  • This study was carried to identify the component of Spleen Meridian Muscle in human, dividing into outer, middle, and inner part. Lower extremity and trunk were opened widely to demonstrate muscles, nerve, blood vessels and the others, displaying the inner structure of Spleen Meridian Muscle. We obtained the results as follows; 1. Spleen Meridian Muscle is composed of the muscle, nerve and blood vessels. 2. In human anatomy, it is present the difference between a term of nerve or blood vessels which control the muscle of Meridian Muscle and those which pass near by Meridian Muscle. 3. The inner composition of meridian muscle in human arm is as follows ; 1) Muscle; ext. hallucis longus tend., flex. hallucis longus tend.(Sp-1), abd. hallucis tend., flex. hallucis brevis tend., flex. hallucis longus tend.(Sp-2, 3), ant. tibial m. tend., abd. hallucis, flex. hallucis longus tend.(Sp-4), flex. retinaculum, ant. tibiotalar lig.(Sp-5), flex. digitorum longus m., tibialis post. m.(Sp-6), soleus m., flex. digitorum longus m., tibialis post. m.(Sp-7, 8), gastrocnemius m., soleus m.(Sp-9), vastus medialis m.(Sp-10), sartorius m., vastus medialis m., add. longus m.(Sp-11), inguinal lig., iliopsoas m.(Sp-12), ext. abdominal oblique m. aponeurosis, int. abd. ob. m., transversus abd. m.(Sp-13, 14, 15, 16), ant. serratus m., intercostalis m.(Sp-17), pectoralis major m., pectoralis minor m., intercostalis m.(Sp-18, 19, 20), ant. serratus m., intercostalis m.(Sp-21) 2) Nerve; deep peroneal n. br.(Sp-1), med. plantar br. of post. tibial n.(Sp-2, 3, 4), saphenous n., deep peroneal n. br.(Sp-5), sural cutan. n., tibial. n.(Sp-6, 7, 8), tibial. n.(Sp-9), saphenous br. of femoral n.(Sp-10, 11), femoral n.(Sp-12), subcostal n. cut. br., iliohypogastric n., genitofemoral. n.(Sp-13), 11th. intercostal n. and its cut. br.(Sp-14), 10th. intercostal n. and its cut. br.(Sp-15), long thoracic n. br., 8th. intercostal n. and its cut. br.(Sp-16), long thoracic n. br., 5th. intercostal n. and its cut. br.(Sp-17), long thoracic n. br., 4th. intercostal n. and its cut. br.(Sp-18), long thoracic n. br., 3th. intercostal n. and its cut. br.(Sp-19), long thoracic n. br., 2th. intercostal n. and its cut. br.(Sp-20), long thoracic n. br., 6th. intercostal n. and its cut. br.(Sp-21) 3) Blood vessels; digital a. br. of dorsalis pedis a., post. tibial a. br.(Sp-1), med. plantar br. of post. tibial a.(Sp-2, 3, 4), saphenous vein, Ant. Med. malleolar a.(Sp-5), small saphenous v. br., post. tibial a.(Sp-6, 7), small saphenous v. br., post. tibial a., peroneal a.(Sp-8), post. tibial a.(Sp-9), long saphenose v. br., saphenous br. of femoral a.(Sp-10), deep femoral a. br.(Sp-11), femoral a.(Sp-12), supf. thoracoepigastric v., musculophrenic a.(Sp-16), thoracoepigastric v., lat. thoracic a. and v., 5th epigastric v., deep circumflex iliac a.(Sp-13, 14), supf. epigastric v., subcostal a., lumbar a.(Sp-15), intercostal a. v.(Sp-17), lat. thoracic a. and v., 4th intercostal a. v.(Sp-18), lat. thoracic a. and v., 3th intercostal a. v., axillary v. br.(Sp-19), lat. thoracic a. and v., 2th intercostal a. v., axillary v. br.(Sp-20), thoracoepigastric v., subscapular a. br., 6th intercostal a. v.(Sp-21)

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Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10

  • Thi Thu Huong Luong;Supattra Poeaim ;Narumon Tangthirasunun
    • Mycobiology
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    • v.51 no.4
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    • pp.239-245
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    • 2023
  • Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the 𝛽-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 ℃ while reaching 61% activity at 65 ℃. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.