• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.123-131
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• 2005

Lipid and protein contents in whole soybean seeds have negative correlation (r=-0.693**), however, these components in seed coat showed positive correlation (r=0.746**). Fatty acids in whole soybean seeds were higher in the order of $C_{18:2}>C_{18:1}>C_{16:0}>C_{18:3}>C_{18:0}$, while those of seed coat were higher in the order of $C_{18:3}>C_{18:2}>C_{18:0}>C_{16:0}>C_{18:1}$. The average content of total amino acid in twenty Korean soybean varieties was 38,938.7 mg/100 g, while that of seed coat was 4,418.4 mg/100g. Glutamic acid showed the highest composition rate $(16.4\%)$ in whole soybean seeds, while glycine was the highest in seed coat and their composition rate was $23.8\%$. The surface of shiny-lustre seed coats was smooth and their pore size was observed smaller than dull-lustre ones. Significant quadratic regression was observed among seed coat lightness, seed coat thickness, protein, lipid, unsaturated fatty acid and crude fiber. Fucose, rhamnose, glucose, mannose, galactose, arabinose and xylose were detected as a neutral mono-saccharides in the seed coats. The arabinose and xylose showed significant correlation with seed coat lightness. The unsaturated fatty acid was significantly correlated with seed coat lightness (r=0.726**). Water absorption rate was low in the thick seed coat varieties, but the rate was high in the shiny seed coat varieties. From the obtained results, it was considered that the thinner and brighter seed coat varieties were much favorable to increase the water absorption rate than thicker and darker seed coat ones.

• Journal of Life Science
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• v.7 no.4
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• pp.355-357
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• 1997

Seed size is a important yield components in soybean (Glycine max L.). The seed size frequency distributions in the mating between two G. max parents possessing quite different seed size exhibited a continuous distribution in the F$_{2}$ and F$_{3}$ generations. A progeny seed size equal to that of either parent was not observed in either generation. The population mean seed size in each generation was less than the mid-parent, with the distribution of lines skewed toward the small seeded parent.

• Preventive Nutrition and Food Science
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• v.8 no.4
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• pp.336-342
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• 2003

The effects of cultivation methods (cultivation of curled-shaped type, M-1; conventional cultivation, M-2; growing after treatment with a growth regulator, M-3; cultivation by the combination of M-1 and treatment with oak charcoal, M-4) on the quality characteristics of soybean sprouts were studied by the measurement of growth characteristics. This study also investigated the changes in shelf-life stability of the new soybean sprouts (NSB) using M-4, which was cultivated with oak charcoal and treated with antimicrobial Citrus sunki seed extract. Among the soybean sprouts grown for six days at the high temperature and humidity environment (90$\pm$5% RH, 25$\pm$1$^{\circ}C$), M-1 revealed no significant difference in terms of quality, such as the harvest yield, the rot rate and the growth characteristics when compared with M-2. M-3 showed no significant difference in growth characteristics, of hardness, and sensory evaluation scores when compared with the soybean sprouts grown by conventional methods. NSB had a low number of total microorganisms and had a better appearance after five days of storage than did the control group (M-2). These findings demonstrate that chemical-free and clean soybean sprouts can be grown by combining oak charcoal and antimicrobial Citrus sunki seed extract, thereby meeting the consumer demand for safe, chemical free sprouts.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.spc
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• pp.78-83
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• 2008

Soybean [Glycine max (L.)] is a major source of protein for human and animal feed. Inter- and intra-genotype variation of soybean protein has been investigated by soybean researchers. However, limited sample amount of soybean single seed there is no report that investigated intra-plant variation of soybean protein within soybean plant. Recently a non-destructive NIR (near-infrared reflectance) spectroscopy using single seed grain to analyze seed protein was developed. The objectives of this study were to understand variation of seed protein content within plant and to determine the amount of minimum sample size which can represent protein content for a soybean plant. Frequency distribution of protein content within plant showed normal distribution. There was an intra-cultivar variation for protein content in soybean cultivar Seonnogkong. Difference of protein content among single plants of Seonnokong was recognized at 5% level. Seeds in lower position on plant stem tended to accumulate more protein than in higher position. There was significant difference for protein content between sample size 5 seeds and sample size of more than 5 seeds (10, 20, 30, 40, and 50 seeds) at a soybean plant with 57 seeds however no difference was recognized among sample size (5, 10, 20, and 30 seeds) at a soybean plant with 33 seeds. Around 20% seeds of soybean from single plant needed to determine the protein content to represent protein content of single soybean plant. This study is the first one to report evidence of intra-plant variation for proteincontent which detected by non-destructive NIR spectroscopy using single seed grain in soybean.

• Journal of Life Science
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• v.7 no.4
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• pp.358-360
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• 1997

Soybean [Glycine max (L.) Merr.] seed size is a important yield component and is a primary consideration in the development of cultivars for specialty markets. Our objective was to examine the consistency of QTLs for seed size across generations. A 68-plant F$_{2} segregation population derived from a mating between Marcury (small seed) and PI 467.468 (large seed) was evaluated with RAPD markers. In the F$_{2} plant generation (i.e. F$_{3} seed), three markers, OPL09a, OPM)7a, and OPAC12 were significantly (P<0.01) associated with seed size QTLs. In the F$_{2} ; F$_{3} generation (i.e., F$_{4} seed), four markers, OPA092, OPG19, OPL09b, and OPP11 were significantly (P<0.01) associated with seed size QTLs. Just two markers, OPL09a, and OPL09b were significantly (P<0.05) associated with seed size QTLs in both generations. The consistency of QTLs across generations indicates that marker-assisted selection for seed size is possible in a soybean breeding program.

• Journal of the Korean Dietetic Association
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• v.26 no.1
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• pp.1-13
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• 2020

This study was undertaken to optimize preparation of sweet rice muffins using lotus (Nelumbo nucifera Gaertn) seed powder. The experimental conditions used in the preparation included lotus seed powder (X₁) and soybean oil (X₂). The muffin formulation was optimized using rheology. Lightness (P<0.01) displayed a quadratic model pattern, whereas yellowness (P<0.01) and redness (P<0.05) was presented a linear model. Texture properties significantly differed in cohesiveness (P<0.05). Evaluating the sensory characteristics of muffins, except for flavor and texture, the remaining properties of color (P<0.01), taste, overall quality, and appearance (P<0.05) were found to be significantly different. The overall quality was also affected by the proportion of lotus seed powder, rather than the amount of soybean oil. The optimal ratio for palatability of muffins was determined to be 47.80 g lotus seed powder and 95.28 g soybean oil. Taken together, our results indicate that sweet rice muffins prepared using lotus seed powder are sufficiently competitive in terms of function and quality.

• The Plant Pathology Journal
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• v.28 no.1
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• pp.55-59
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• 2012

Northern stem canker caused by $Diaporthe$ $phaseolorum$ var. $caulivora$ ($Dpc$) has become a serious disease in soybean. The objectives of this study were to survey the existence of $Dpc$ on soybean in Korea, and to examine the potential pathogenicity of $Dpc$ in seed decay. One such isolate, SSLP-4, isolated from a field-grown plant of the Korean soybean cultivar Danbaekkong, was identified as $Dpc$, based on its morphological and molecular characteristics by sequences of internal transcribed spacer (ITS), translation elongation factor (TEF) 1-${\alpha}$ and ${\beta}$-tubulin regions, as well as pathogenic analyses. Moreover, morphological and molecular analyses revealed that isolate SSLP-4 was nearly identical to $Dpc$ strains from the United States. Pathogenicity tests on hypocotyls of soybean seedlings and detached leaves resulted in typical symptoms of soybean northern stem canker and inoculation on plants at R5-R7 stage caused seed decay. All results suggest that the $Dpc$ strain SSLP-4 can cause both stem canker and seed decay on soybean. Thus, the SSLP-4 isolate has the potential to contribute greatly to understanding of host plant resistance mechanisms, both at vegetative and reproductive growth stages in soybean.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.4
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• pp.331-336
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• 2004

Changes in the level of metabolites in leaves and pods were examined with respect to the seed chemical composition in black soybean. There was no further increase in pod length after 42 days after flowering (DAF). Pod weight, however, persistently increase until 73 DAF, thereafter the weight was slightly lowered. The seed storage protein, however, increased drastically as the increasing rate of pod weight was lessened at 61 DAF. The accumulation of seed storage proteins was occurred conspicuously as the increasing rate of pod weight was slowed down. The chlorophyll content both in leaves and pods was drastically decreased after 50 DAF. The beginning of drastic reduction in chlorophyll content was occurred concomitantly with the reduction of soluble protein content in leaves. The sugar content in leaves showed similar tendency with chlorophyll and soluble protein content. The starch level in leaves, however, showed different changing pattern during seed development. The starch content in leaves was increased persistently until 66 DAF, thereafter the content was decreased drastically to about $55\%$ of maximal value at 66 DAF. Total phenolics content in leaves and the anthocyanins content in seeds were stable without noticeable increase until 66 DAF. The contents were increased dramatically after 66 DAF showing the synchronized pattern with the decrease in starch level in leaves. The levels of the selected metabolites in leaf and seed suggested that the accumulation of chemical components of black soybean seed is launched actively at 66 DAF. The profile of storage proteins was nearly completed at 61 DAF because there was no large difference in densitometric intensity among protein subunits after 61 DAF. In soybean, chemical maturation of seed begins around 61 to 66 DAF at which most metabolites in vegetative parts are decreased and remobilized into maturing seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.141-146
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• 1998

Production of medicinal soybean [Glycine max (L.) Merrill], characterized with black seed, white stripe at hilum border, yellow cotyledon and small seed, is increasing with increasing consumption. The objective of this study was to investigate the chemical composition of medicinal soybean seed and to provide basic information, for the characterization of these soybeans among genetic resources. Forty-four lines of medicinal soybeans collected from Korea and two control cultivars, 'Hwangkeumkong' (Yellow seed coat) and 'Geomjeongkong l' (Black seed coat) were planted at the Research Farm of the College of Natural Resources, Korea University, located at Namyangju City on May 25, 1996. Seeds of these lines were harvested at full maturity and analyzed for protein, oil, sugar, starch and mineral contents. Mean protein and oil content of the medicinal line seeds were 42.6 and 16.1%, respectively, and those of the control cultivars were in the middle range for protein and oil content. However, sugar and starch content of the medicinal line seeds appeared to be in the lower range of the distributions compared to the control cultivars and were 10.0 and 1.68%, respectively. Mean P, K, Ca, and Mg contents of the seeds of medicinal soybean lines were 15.9, 21.5, 3.11, and 2.81 mg/g, respectively, indicating that these lines had higher P, K, and Mg and lower Ca contents when compared to the control cultivars. Mean Na and Fe contents were 671 and 224 mg/kg, respectively, showing lower Na and similar Fe contents. The observed results provided that chemical compositions of medicinal soybean were, on average, different from those of the general soybean cultivars.

• Journal of Biosystems Engineering
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• v.42 no.3
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• pp.227-233
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• 2017

Purpose: Morphological properties of soybean roots are important indicators of the vigor of the seed, which determines the survival rate of the seedlings grown. The current vigor test for soybean seeds is manual measurement with the human eye. This study describes an application of a machine vision technique for rapid measurement of soybean seed vigor to replace the time-consuming and labor-intensive conventional method. Methods: A CCD camera was used to obtain color images of seeds during germination. Image processing techniques were used to obtain root segmentation. The various morphological parameters, such as primary root length, total root length, total surface area, average diameter, and branching points of roots were calculated from a root skeleton image using a customized pixel-based image processing algorithm. Results: The measurement accuracy of the machine vision system ranged from 92.6% to 98.8%, with accuracies of 96.2% for primary root length and 96.4% for total root length, compared to manual measurement. The correlation coefficient for each measurement was 0.999 with a standard error of prediction of 1.16 mm for primary root length and 0.97 mm for total root length. Conclusions: The developed machine vision system showed good performance for the morphological measurement of soybean roots. This image analysis algorithm, combined with a simple color camera, can be used as an alternative to the conventional seed vigor test method.