The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Diaporthe phaseolorum var. caulivora, a Causal Agent for Both Stem Canker and Seed Decay on Soybean

  • Sun, Su-li (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Van, Kyu-Jung (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Moon-Young (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Min, Kyung-Hun (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University) ;
  • Lee, Yin-Won (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University) ;
  • Lee, Suk-Ha (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Received : 2011.10.17
  • Accepted : 2011.11.30
  • Published : 2012.03.01
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Abstract

Northern stem canker caused by $Diaporthe$ $phaseolorum$ var. $caulivora$ ($Dpc$) has become a serious disease in soybean. The objectives of this study were to survey the existence of $Dpc$ on soybean in Korea, and to examine the potential pathogenicity of $Dpc$ in seed decay. One such isolate, SSLP-4, isolated from a field-grown plant of the Korean soybean cultivar Danbaekkong, was identified as $Dpc$, based on its morphological and molecular characteristics by sequences of internal transcribed spacer (ITS), translation elongation factor (TEF) 1-${\alpha}$ and ${\beta}$-tubulin regions, as well as pathogenic analyses. Moreover, morphological and molecular analyses revealed that isolate SSLP-4 was nearly identical to $Dpc$ strains from the United States. Pathogenicity tests on hypocotyls of soybean seedlings and detached leaves resulted in typical symptoms of soybean northern stem canker and inoculation on plants at R5-R7 stage caused seed decay. All results suggest that the $Dpc$ strain SSLP-4 can cause both stem canker and seed decay on soybean. Thus, the SSLP-4 isolate has the potential to contribute greatly to understanding of host plant resistance mechanisms, both at vegetative and reproductive growth stages in soybean.

Keywords

References

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