KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Changes of Chemical Components During Seed Development in Black Soybean (Glycine max L.)

  • Shim Sang In (Division of Plant Resources & Environment, Gyeongsang National University, Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kang Byeung Hoa (Division of Environmental Science & Ecological Engineering, Korea University)
  • Published : 2004.09.01
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Abstract

Changes in the level of metabolites in leaves and pods were examined with respect to the seed chemical composition in black soybean. There was no further increase in pod length after 42 days after flowering (DAF). Pod weight, however, persistently increase until 73 DAF, thereafter the weight was slightly lowered. The seed storage protein, however, increased drastically as the increasing rate of pod weight was lessened at 61 DAF. The accumulation of seed storage proteins was occurred conspicuously as the increasing rate of pod weight was slowed down. The chlorophyll content both in leaves and pods was drastically decreased after 50 DAF. The beginning of drastic reduction in chlorophyll content was occurred concomitantly with the reduction of soluble protein content in leaves. The sugar content in leaves showed similar tendency with chlorophyll and soluble protein content. The starch level in leaves, however, showed different changing pattern during seed development. The starch content in leaves was increased persistently until 66 DAF, thereafter the content was decreased drastically to about $55\%$ of maximal value at 66 DAF. Total phenolics content in leaves and the anthocyanins content in seeds were stable without noticeable increase until 66 DAF. The contents were increased dramatically after 66 DAF showing the synchronized pattern with the decrease in starch level in leaves. The levels of the selected metabolites in leaf and seed suggested that the accumulation of chemical components of black soybean seed is launched actively at 66 DAF. The profile of storage proteins was nearly completed at 61 DAF because there was no large difference in densitometric intensity among protein subunits after 61 DAF. In soybean, chemical maturation of seed begins around 61 to 66 DAF at which most metabolites in vegetative parts are decreased and remobilized into maturing seeds.

Keywords

References

  1. Arnon, D I 1949 Copper enzyme in isolated chloroplasts polyphenol oxidase in Beta vulgaris Plant Physiol 24 1-15 https://doi.org/10.1104/pp.24.1.1
  2. Bradford, M M 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utihzing in principle of protein-dye binding Anal Biochem 72. 248-254
  3. Cai, T and K C Chang. 1999 Processing effect on soybean storage proteins and their relationship with tofu quality J. Agric Food Chem. 47 729-727
  4. Choi, Y B., K. S KIm, K H. Lee, and H S. Sohn 1995. Development soy oligosaccharides. Korea Soybean Digest 12 68-74
  5. Ciha, A J. and W A Brun. 1978 Effect of pod removal on nonstructural carbohydrate levels in soybeans. Agron. J 69 . 965-969 https://doi.org/10.2134/agronj1977.00021962006900060016x
  6. Dreccer, M. F., M van Oijen, A H C. M Schapendonk, C S. Pot, and R Rabbinge 2000 Dynamics of vertical leaf nitrogen distribution in a vegetative wheat canopy. Impact on canopy photosynthesis, Annals Bot. 86 821-831 https://doi.org/10.1006/anbo.2000.1244
  7. Egli, D. B ,J. E Leggett, and A Cheniae 1980. Carbohydrate levels in soybean leaves during reproductive growth Crop Sci 20 . 468-473 https://doi.org/10.2135/cropsci1980.0011183X002000040012x
  8. Giaquinta, R. T ,B Quebedeaux, N L. Salder, and V R Francesch 1984 Assimilate partitioning in soybean leaves during seed filling World Soybean Research Conference II pp. 729-738
  9. Goldbohm R. A ,M G L. Hertog, H. A M Brants, G van Poppel, and P A van den Brandt 1998. Intake of flavonoids and cancer risk: a prospective cohort study CoOST 916 Bioactive Plant Cell Wall Components in nutition and Health: Polyphenols in Food. p 166
  10. HilI, J. E and R W. Breidenbach. 1974 Proteins of soybean seed I. Isolation and characterization of the major components Plant Physiol. 53 742-746 https://doi.org/10.1104/pp.53.5.742
  11. Holt, S 1997 Soya The health food of the next millennium, Korea Soybean Digest. 14. 77-90
  12. Kang, L J , Y. Matsumura, and T. Mori 1991 Characterization of texture and mechanical properties of heat-induced soy protein gels. J Amer. Oil Chem Soc. 68 339-345 https://doi.org/10.1007/BF02657690
  13. Kim,J, H., Y. Yoshiki, and K. Okubo. 1994 Relationships among various types in saponin content of soybean seeds. Korea Soybean Digest 11 .55-61
  14. Kim, J. S 1996 Current research trends on bioactrve function of soybean Korea Soybean Digest. 13 : 17-24
  15. Kim, S. L ,H T Yun, J K. Moon, K Y.Park, Y.H Lee, and Y H Ryu. 2004 Variations in seed storage protein among different colored soybean varieties Korean J. Crop Sci 49 : 141-147
  16. Kim, Y H 2003 Biological activities of soyasaponins and their genetic and environmental variations in soybean. Korean. J. Crop Sci 48 . 49-57
  17. Kolde, T 1997. Antitumor effect of anthocyanin fractions extracted from red soybean and red beans in vitro and in vivo Cancer Biotherapy and Radiophamaceuticals 12.277-280
  18. Kyoko, D 1998 Soybean food industry of the 21st century Korea Soybean Digest 15. 94-105
  19. Laemmli, U K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680-685 https://doi.org/10.1038/227680a0
  20. Latimore, M., J Giddens, and D A. Ashley. 1977 Effect of ammomum and nitrate nitrogen upon photosynthate supply and nitrogen fixation by soybeans Crop Sci. 17 399-404 https://doi.org/10.2135/cropsci1977.0011183X001700030015x
  21. Milroy, S P. and M. P Bange 2003. Nitrogen and light responses of cotton photosynthesis and implications for crop growth. Crop Sci. 43.904-913 https://doi.org/10.2135/cropsci2003.0904
  22. Mondal, M H., W A Brun, and M. L Brenner. 1978 Effect of sink removal on photosynthesis and senescence in leaves of soybeans (Glycine max L) plants. Plant Physiol 61 : 349-397
  23. Murphy, P. A and A P. Resurreccion, 1984 Varietal and environmental differences in soybean glycinin and $\beta$-conglycinin content. J. Agric Food Chem. 32 . 911-915 https://doi.org/10.1021/jf00124a052
  24. Oakenfull, D 1981. Saponins m food Food. Chem 7: 19-40 https://doi.org/10.1016/0308-8146(81)90019-4
  25. Pate, J. S., P. J Sharkey, C A Atkins 1977 Nutrition of a developing legume fruit: Functional economy in terms of carbon, nitrogen, water Plant Physiol 59 506-510 https://doi.org/10.1104/pp.59.3.506
  26. Pool-Zobel, B L., A Bub, N. Schrer, and G Rechlrmmer. 1999. Anthocyanins are potent antioxidants in model systems but do not reduce endogenous oxidative DNA damage in human colon cells Eur J. Nutr 38. 227-234 https://doi.org/10.1007/s003940050065
  27. Rossato, L , P Laine, and A. Ourry. 2001 Nitrogen storage and remobilization in Brassica napus L during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns J. Exp Bot 52: 1655-1663 https://doi.org/10.1093/jexbot/52.361.1655
  28. Saleem A, E Loponen, K Pihlaja, and E Oksanen 2001 Effects of long-term open-field ozone exposure on leaf phenolics of European silver birch (Betula pendula Roth). J Chem Ecol. 27(5) 1049-1062 https://doi.org/10.1023/A:1010351406931
  29. Seigelman, H W and S B. Hendricks 1958 Photocontrol of alcohol, aldehyde and anthocyanin production in apple skin. Plant Physiol. 33 409-413 https://doi.org/10.1104/pp.33.6.409
  30. Seong, R C. and J H Park 1993 Effects of leaf and pod removal on assimilate translocation in soybean plant Korean J. Crop Sci 38: 377-382
  31. Staswick, P. E. 1994. Storage proteins of vegetative plant tissues Annu Rev Plant Physiol Plant Mol BioI. 45 . 303-322 https://doi.org/10.1146/annurev.pp.45.060194.001511
  32. Taira, H 1972 Influence of Iocation on the chemical composition of soybean seeds Jpn J. Crop Sci 41 . 235-243 https://doi.org/10.1626/jcs.41.235
  33. Wang, H., G Cao, and R L Prior 1997. Oxygen radical absorbing capacity of anthocyanin, J Agric Food Chem. 45 304-30 https://doi.org/10.1021/jf960421t
  34. Waterman, P. G and S Mole. 1994. Analysis of Plant Metabolites, Methods in Ecology Series, Blackwell Scientific Publications, Oxford
  35. Wrnkel-Shirley, B. 2002. Biosynthesis of flavonoids and effect of stress. Curr Opm, Plant BioI 5 218-223
  36. Yoshida, S., D A. Forno, F H Cook, and K A Gomez. 1976 Laboratory manual for physiological studies of rice IRRI pp 46-49