• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.51 no.4
• /
• pp.310-317
• /
• 2006

This study was conducted to investigate the influence of drought stress during the pod developing and seed filling stage on source-sink relationships of soybean (Glycine max). Drought treatments were imposed by withholding water at the full-pod stage, 19 days after flowering, and then limited watering was relieved at 15 days after the initiation of drought treatment. Soybean seed yield was reduced by 39% mainly due to decreased pod number under drought stress, but the 100-seed weight was relatively less reduced. In spite of the 15-day drought during the full-pod stage, soybean produced good seeds showing similar l00-seed weight, protein, starch and soluble sugar content to those from the well-watered. Although drought during the full-pod stage caused source limitations; i.e. accelerated leaf senescence and reduced leaf soluble sugars, it did not cause limitations of other source characteristics such as SGR and leaf starch level. This is because the reduction in size of sinks, such as pod and seed abortions compensated for source limitations, resulting in balanced source-sink as expressed by LAR and the ratio of leaf area to seed dry weight. Drought stress during the pod developing and seed filling stage did not disrupt the source-sink balance

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.1
• /
• pp.25-30
• /
• 2003

This research was conducted to compare the dry matter production and the yield productivity among nine soybean cultivars by measuring the photosynthetic ability of the middle and lower leaves at the flowering and the seed development stages. The leaf greenness(SPAD value) were ranged as 32-42 at the flowering stage. Also, They were ranged as 25-40 and 38-51 at the fifth leaf and the seventh leaf, respectively. The photosynthetic ability at the flowering and the seed development stage showed significant differences among soybean cultivars, and the photosynthetic ability at the seed development stage showed higher difference among cultivars than the flowering stage. The variation of the photosynthetic ability at the flowering and the seed development stage also was significant among cultivars. The light saturation point at the flowering stage was about 1500 $\mu$mol $m^{-2}$ $s^{-1}$ PAR, and the seed development stage was about 1000 $\mu$mol $m^{-2}$ $s^{-1}$ PAR. The photosynthesis showed the high negative correlation with the leaf area and the positive correlation with the leaf area ratio. Also, photosynthesis at seed development stage showed positive correlation with grain yields but there was not significant between photosynthesis and yields at flowering stage..

• Korean journal of applied entomology
• /
• v.44 no.4 s.141
• /
• pp.299-306
• /
• 2005

Soybean seed injury was analyzed in the experiments that soybean pods were allowed to be sucked by adults of the bean bug, R. clavatus, and were picked with a specimen pin. While attack by the bean bug at podding stage of soybean caused the increase of empty pods and completely-undeveloped seeds, attack at full seed stage caused the increase of seeds wi distinct injury mark on seed-coat. The ratio of deformed seed was lower than those of injury-marked seed and undeveloped seed when attacked during all stages. In at full bloom stage hardly produced injury-marked seeds and deformed seeds. When the injured seeds were dyed with acid-fuchsine solution, stylet sheaths of R. clavatus formed on seed-coat were observed in 83% of undeveloped seeds formed under attack during podding stage and in 91% of injury-marked seeds formed during full seed stage. In pods injured with a specimen pin at full seed and full maturity stages, no healthy seeds could be obtained from the pods. And the more picked at full seed stage, the more deformed seeds were produced and the higher weight reduction of injury-marked seed occurred. However, pin-injury at full maturity stage didn't give rise to weight reduction of seeds.

• The Plant Pathology Journal
• /
• v.38 no.4
• /
• pp.313-322
• /
• 2022

Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. It has been well established during the last few decades that plant seeds harbor diverse microbes, some of which are vertically transmitted and important for plant health and productivity. In this study, we isolated culturable endophytic bacteria and fungi from soybean seeds and evaluated their antagonistic activities against common bacterial and fungal seed-borne pathogens of soybean. A total of 87 bacterial isolates and 66 fungal isolates were obtained. Sequencing of 16S rDNA and internal transcribed spacer amplicon showed that these isolates correspond to 30 and 15 different species of bacteria and fungi, respectively. Our antibacterial and antifungal activity assay showed that four fungal species and nine bacterial species have the potential to suppress the growth of at least one seed-borne pathogen tested in the study. Among them, Pseudomonas koreensis appears to have strong antagonistic activities across all the pathogens. Our collection of soybean seed endophytes would be a valuable resource not only for studying biology and ecology of seed endophytes but also for practical deployment of seed endophytes toward crop protection.

• Korean Journal of Breeding Science
• /
• v.43 no.4
• /
• pp.311-317
• /
• 2011

This experiment was conducted to give basic information to sprout-soybean breeding and automated sprout production by investigating the effect of seed size on the sprout growth and texture. Twenty cultivars and lines including large and medium soybean, small interspecific cultivars and extra-small wild soybean lines were used. Seeds were cultured for 4 days using small sprout-culturing kits. Hypocotyl length of large Hwanggeumkong, small Pungsannamulkong and Soyoung were longer and wild soybean lines were shorter than other cultivars. Fresh sprout weight and growth rate per unit dry seed weight of wild soybean lines ware increased dramatically and more than other cultivars between 48 to 72 hours after culture. Not only the increasing rate but sprout yield ratio to used dry seed weight showed the negative relationship pattern with seed size. The hardness of hypocotyl in Jangyeubkong, mastication in Taekwang, and cutting force in Eunhakong were the highest as 3,505 g, 1,650 g, and 133 g respectively, and texture values of these traits in wild soybean YWS516 were the lowest. Cutting force of soybean hypocotyl showed the positive relationship pattern with seed size. Breaking force of hypocotyl in large Jangyeobkong was the highest as 83.5g and wild soybean lines were the lowest showing the same pattern as other textural characters.

• Microbiology and Biotechnology Letters
• /
• v.11 no.3
• /
• pp.241-248
• /
• 1983

Lupin seed was used to make Meju, the fermentation starter for Korean soybean sauce and paste in substitution for soybean and the fermentation characteristics were compared with those of soybean. Mejus were prepared by in-oculating Asp. oryzae on the cooked whole beans. The dried Mejus were used for making fermented bean sauce and paste by mixing with brine and subsequent ripening for 4 weeks. In general the protease activity and amylase activity-during ripening were higher in lupin seed Meju than those of soybean Meju. The increase in protease activity correlated to the increase in $\alpha$-amino nitrogen content of the fermented paste and sauce. The development of dark-brown color of the sauce during ripening faster with lupin seed Meju compared to soybean Meju. In sensory evaluation the flavor score of lupin seed sauce and paste was slightly lower than that of soybean products but the overall quality of fermented lupin seed sauce was acceptable.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.3
• /
• pp.359-363
• /
• 2014

Soybean seed is a good source of plant protein in human consumables such as baby formula and protein concentrate. The seeds contain an abundance of storage proteins, namely ${\beta}$-conglycin and glycinin that account for ~ 70-80% of the total seed protein content. Proteome profiling has been proved to be an efficient way that can help us to investigate the seed storage proteins. In the present study, the seeds were removed from the pods and the cotylendonary tissues were separated from the testa for proteome analysis in order to investigate the seed storage proteins. A systematic proteome profiling was conducted through one-dimensional gel electrophoresis followed by MALDI-TOF-TOF mass spectrometry in the seeds (cotyledonary tissue) of soybean genotypes. Two dimensional gels stained with CBB, a total of 10 proteins were identified and analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. A total of ten proteins such as glycinin Gy4 precursor, glycinin G3 precursor, glycinin G1 precursor, glycinin chain A2B1a precursor, glycinin chain A2B1a precursor were identified in our investigation. However, the glycinin subunit may be considered to play important roles in soybean breeding and biochemical characterization. In addition, the improved technique will be useful to dissect the genetic control of glycinin expression in soybean.

• Journal of Plant Biotechnology
• /
• v.45 no.4
• /
• pp.328-332
• /
• 2018

Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrate, isoflavone and other various nutrients for humans and animals. However, there are anti-nutritional factors in the raw mature soybeans. Kunitz trypsin inhibitor (KTI) protein and stachyose are the main anti-nutritional factors in soybean seed. The ${\alpha}^{\prime}$-subunit of ${\beta}$-conglycinin protein exhibit poor nutritional and food processing properties. The genetic removal of the KTI and ${\alpha}^{\prime}$-subunit proteins will improve the nutritional value of the soybean seed. The objective of this research was to develop a new soybean strain with KTI and ${\alpha}^{\prime}$-subunit protein free ($titicgy_1cgy_1$ genotype) and proper agronomic traits. A breeding population was developed from the cross of the Bl-1 and 15G1 parents. A total of 168 $F_2$ seeds from the cross of the BL-1 and 15G1 parents were obtained. The segregation ratios of 9: 3: 3: 1 ($104Ti\_Cgy_{1\_}:\;30Ti\_cgy_1cgy_1:\;21cgy_1cgy_1Ti\_:\;13titicgy_1cgy_1$) between the Ti and $Cgy_1$ genes in the $F_2$ seeds were observed (${\chi}^2=5.12$, P=0.5-0.10). Two $F_4$ plant strains with proper agronomical traits and $titicgy_1cgy_1$ genotype (free of both KTI and ${\alpha}^{\prime}$-subunit protein) were selected and harvested. 2 strains (S1 and S2) had yellow seed coats and hilum. The plant height of the S1 strain was 65 centimeters. The 100-seed weight was 29.2 g. The plant height of the S2 strain was 66 centimeters and 100-seed weight was 26.2 g. The two strains selected in this research will be used to improve the new cultivar that will be free of the KTI and ${\alpha}^{\prime}$-subunit proteins.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.1
• /
• pp.44-51
• /
• 1995

This investigation was conducted to determine the effect of the optimum planting date and place for good seed production on growth variation, grain yields, different of variety, infection of seed by fungi, and seed germination and vigor after room storage. Early maturity Korean soybean variety, Keunolkong, and introduced vegetable soybean variety, Fukura-edamame, were planted at field of YAES. and high cool land of Sajapyong in Milyang（altitude, 850m） on May 15 （early）, June 15 （mid.）, and July 15 （late） in 1991. The emergence and vegetative period gradually increased in the early planting date. The grain yield, seed weight, pod number, healthy grain yield also increased when Keunolkong was planted on early date. The healthy seed rate, Keunolkong increased in the early planting date, whereas that of Fukura also increased in the late planting date. Infection ratio of grain to phomopsis seed decay （Phomopsis spp. ' Diaporthe phaseolorum） in Fukura steadily increased in the early planting date. Infection ratio of grain to purple seed stain （Cercospora kikuchii） generally increased in the mid and late planting date. The seed germination and seedling vigor after room storage from five to six months gradually increased in the late planting date. And seed germination of Fukura rapidly decreased in the early planting date. Therefore, the optimum planting date for good seed production in early maturity vegetable soybean was June 15 in terms of harvesting time avoid a high temprature and humidity.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.15-15
• /
• 2021

Seed coat color and seed weight are among the key agronomical traits that determine the nutritional quality of soybean seeds. This study aimed to evaluate the contents of total protein, total oil and five prominent fatty acids in seeds of 49 soybean varieties recently cultivated in Korea, and assess the influences of seed coat color and seed weight on each. Total protein and total oil contents were in the ranges of 36.28-44.19% and 13.45-19.20%, respectively. Likewise, individual fatty acid contents were in the ranges of 9.90-12.55, 2.45-4.00, 14.97-38.74, 43.22-60.26, and 5.37-12.33% for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. Our results found significant variations of protein, oil and fatty acid contents between the soybean varieties. Moreover, both seed coat color and seed weight significantly affected total oil and fatty acid contents. Total protein content, however, was not significantly affected by any factor. Among colored soybeans, pale-yellow soybeans were characterized by a high level of oleic acid (30.70%) and low levels of stearic (2.72%), linoleic (49.30%) and linolenic (6.44%) acids, each being significantly different from the rest of colored soybeans (p < 0.05). On the other hand, small soybeans were characterized by high levels of all individual fatty acids except oleic acid. The level of oleic acid was significantly high in large seeds. Cluster analysis grouped the soybeans into two classes with notable content differences. Principal component analysis also revealed fatty acids as the prime factors for the variability observed among the soybean varieties. As expected, total oil and total protein contents showed a negative association with each other (r = -0.714, p < 0.0001). Besides, oleic acid and linoleic acid showed a tradeoff relationship (r = -0.936, p < 0.0001) which was reflected with respect to both seed coat color and seed weight. In general, the results of this study shade light on the significance of seed coat color and seed weight to distinguish soybeans in terms of protein, oil and fatty acid contents. Moreover, the soybean varieties with distinct characteristics and nutritional contents identified in this study could be important genetic resources for consumption and cultivar development.