• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.335-339
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• 2003

Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora 'Shuho-no-chikara' by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days'early flowering in field.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.379-394
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• 2011

This study was carried out to classify the folk plants in southern region of Gangwon-do. According to the survey results from 1,103 survey sheets of 83 residents at 32 places of 9 counties and cities from March to October, 2010, the folk plants in the southern region of Gangwon-do consisted of a total 311 taxa; 92 families, 222 genera, 274 species, 2 subspecies, 29 varieties, and 6 form. The use by its usage was: 227 taxa; Edible, 174 taxa; Medicinal, 14 taxa; Ornamental, 5 taxa; Oil, 5 taxa; Spice, 4 taxa; Dye, 1 taxa; Fiber, 1 taxa; Aroma, 1 taxa; Fuel, 32 taxa; Others, respectively, so the edible use is the highest. The most useful part was the leaf, followed by root and fruit. The consistency comparison between the scientific name and the local name were the highest in the 50's and the lowest in 80's.

• Plant Resources
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• v.6 no.1
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• pp.11-15
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• 2003

To determine as optimal sowing time of forage rape in spring time in southern areas of Korea, forage rape cv. Velox, the highest yielding variety among introduced varieties of forage rape, was grown under five different sowing times. Yield components such as plant length, number of branches and number of leaves etc. were higher at the sowing time of Mar. 15 and Mar. 25. The plants sown at Mar. 15 and Mar. 25 also showed highest fresh and dry matter yield. When plants were grown under later sowing time, they showed higher values in content of crude protein and lower values in contents of crude fiber such as Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose and lignin. There was no relationship between variation of In Vitro Dry matter Digestibility (IVDMD) and sowing time. The plants sown at Mar. 15 and Mar. 25 showed highest digestible dry matter yields.

• Journal of Plant Biotechnology
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• v.36 no.3
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• pp.224-229
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• 2009

Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.

• Journal of Microbiology and Biotechnology
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• v.7 no.2
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• pp.138-143
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• 1997

We have designed and constructed a gene encoding novel high essential amino acid encoding protein(HEAAE). The resultant DNA fragment was tested for in vitro and in vivo expression and then cloned into plant expression vector pBI121, under the control of the cauliflower mosaic virus 35S promoter. Agrobacterium tumefaciens, strain LBA4404, was subsequently transformed with this new construct and Nicotiana tabacum var. Xanthi transgenic plants were obtained. DNA analysis by Southern procedure confirmed the presence of the multi-copy number of genes in the transformed plants. Analysis of RNA and protein synthesized in these transgenic plants demonstrated the stable expression of this gene.

• Plant Resources
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• v.3 no.2
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• pp.152-162
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• 2000

This study was performed to investigate the resources flora of Mt. ManRoi(611.7m; 37$^{\circ}$51'N, 127$^{\circ}$ 20'E), during April 1995 to September 1998. The vascular plants, which were collected from the mountain were composed of all 382 taxa including cultivated species, and classified into 1 phylum, 4 classes, 34 orders, 87 families, 254 genera, 323 species, 1 subspecies,55 varieties and 4 forms. There were not rare and endangered species, while 7 taxa of Korean endemic plants, that is, Tricyitis dilatata, Clematis trichotoma, Deutzia coreana, Spiraea prunifolia var, simpliciflora, Forsythia koreana, Weigela subsessilis and Patrinia sanicalaefalia were distributed in this mountain. The vegetation of this investigated area is regarded as the boundary of the temperate middle part and temperate southern part in floral zone of the Korean Peninsula.

• Plant Resources
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• v.7 no.3
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• pp.170-173
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• 2004

To determine optimal plant density of forage rye in southern areas of Korea, forage rye cv. Paldanghomil, the highest yielding variety among experimental varieties of forage rye, was grown under five different plant density. Yield components such as plant height, and number of leaves were highest at the broad casting. Plants sown at broadcasting also showed highest fresh and dry matter yield. When plants were grown of the broad casting, they showed higher values in content of crude protein and lower values in contents of crude fiber such as neutral detergent fiber(NDF), acid detergent fiber(ADF), cellulose and lignin. There was no relationship between variation of in vitro dry matter digestibility(IVDMD) and plant density. Plants sown at the broad casting showed highest digestible dry matter yields.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.180-185
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• 2015

Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.75-80
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• 2009

In order to develop transgenic sweetpotato plants [Ipomoea batatas (L.) Lam. cv. Yulmi] with enhanced tolerance to oxidative stress, we constructed transformation vectors expressing 2-Cys peroxiredoxin (Prx) gene under the control of the stress-inducible SWPA2 or enhanced 35S promoter (named as SP or EP). Transgenic sweetpotato plants were attempted to generate from embryogenic calli using an Agrobacterium-mediated transformation system. Embryogenic calli gave rise to somatic embryos and then converted into plantlets on MS medium containing 100 mg/L kanamycin. Transgenic plants were regenerated in the same medium. Southern blot analysis confirmed that the Prx gene was inserted into the genome of the plants. To further study we selected the transgenic plant lines with enhanced tolerance against methyl viologen (MV). When sweetpotato leaf discs were subjected to methyl MV at $20{\mu}M$, transgenic plants showed about 40% higher tolerance than non-transgenic or empty vector-transformed plants.

• Korean Journal of Plant Resources
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• v.6 no.1
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• pp.33-44
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• 1993

The medicinal plants used traditional medicines are classified into two groups. The first group is formulary medicine which have been recorded on authorized books, for example, kampo drug in Japan. The second group is the fork medicines. These medicinal plants production is 165 billion yen(about 1.5 billion dollar) and which rate to to total pharmaceutical production is about 3% in 1990. The number of important medicinal plants treated in the market is 124 herbs from the total 364 herbs for kampo durgs(210 preparations kampo durgs) and other propritetary drugs (agents for common cold, laxatives, gastrointestional and so on). The production of the Ginseng and Bupleurum Root are increased about ten times in last twelve years(1976 or 1988). The 80% of these medicinal plants are imported from China, Korea and Southern Asian countries. Then the Pharmaceutical Affairs Bureau of Ministry of Health and Welfare published "The Guideline of Medicinal Plant for Cultivation" for a quality control and a stabilization supply of 80 herbs. The first part(1992) of the guideline reported 5 herbs; Coptis Rhizome, Rehmannia Root, Bupleur, Root, Japanese Angelica Root and Rhubarb, The next part will be published following 5herbs; Cnidium Rhizome, Safflower, Coix seed and Fruiet, Platicodon Root and Zedoary.