• Korean Journal of Environment and Ecology
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• v.15 no.3
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• pp.224-229
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• 2001

The vascular plants in Chilsan islet were summarized as 84 taxi; 37 families, 74 genus, 71 species, 13 varieties. Evidence from the species distribution such as Cudrania tricuspidata and Mallotus japonicus, this islet belongs to the Southern province in phytogeographical point of view. The dominant species in the studied areas are in the follows; Both Carex boottiana and Artemisia carpillaris are in the Islet I, Carex boottiana for the Island II, Both Carex boottiuna and Mallotus japonicus fur the Island III. Clerodendron trichotomum for the Island IV. Both Miscanthus sinensis and Imperate cylindrie var. koenigii for the Island Ⅵ, respectively.

• Korean Journal of Environment and Ecology
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• v.16 no.4
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• pp.359-386
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• 2003

The vascular plants in Baekdudaegan of the South Korea were recorded of 1,477 taxa; 126 families, 641 genus, 1,248 species, 3 subspecies, 204 varieties, 22 forma. From the floristic point of view, Jirisan, Backunsan and Duckyusan District in the Baekdudaegan belongs to the southern province in Korea, appeared to Cephalotaxus koreana, Lindera obtusiloba, Lindera erythrocarpa, Lindera glauca, Izex macropoda, Meliosma myriantha, Melisoma oldhami and Stewartia koreana. But on the top regions of these mountains more than 1,000m above the sea level were contained many alpine plants. And on the top regions and ridgeline parts of Duckyusan and Jirisan were the middle province, owing to Betula costata, Betula ermani, Ulmus laciniata, Isopyrum raddeanum, Hylomecon vemale, Viola diamantica, Vaccinium koreanum, Heloniopsis orientalis, Disporum ovale, Symplocarpus renifolius and Veronica arvensis. These results were epresented that plant species of the middle province moved to the South along the ridgeline of Baekdudaegan, on the other hand plants of southern province had biological niche of low ground according to go toward the North.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.285-292
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• 1997

This study was conducted to obtain the transgenic Brnssica napus plants with tobacco Apase gene using the binary vector system of Agrobacteriurn fumefociens. The results obtained were summarized as follows: A repressible acid phosphatase gene of Saccharon~yces cerevisiae, pho105 was used for screening of tobacco Apase cDNA. In order to identify Apase gene in tobacco genome, Southern blot analysis was pcrformed and the Apase gcnc may be present as a single copy, or at most two or three copies, in tobacco genome. To isolate the tobacco Apase gene, tobacco cDNA library was constructed using purifed mRNA from -Pi treated tobacco root and the plaque forming unit of the library was 2.8 x $10^5$ pfu/m${\ell}$, therefore the library might cover all expressed mRNAs. Using pho5 as a probe. tobacco Apase cDNA was cloned, and restriction mapping and Southern blot analysis of cDNA insert were revealed that the 3.6 kb cDNA contained tobacco acid phosphatase cDNA. Plasmid pGA695 -tcAPl was constructed by subcloning tobacco Apase cDNA into the Hind site of pGA695 with 35s promoter which can be expressed constitutively in plants. The Brassica napus cotyledonary petioles were cocultivated with the ,4 grobacteriunz and transferred to the selection medium. The transformed and regenerated plants were transplanted to soil medium. Southern blot analysis was done on the transformed plants, and it was confirmed that a foregin gene was stably integrated into the genonies of B. nnpus plants.

• Journal of Plant Biology
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• v.17 no.2
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• pp.84-86
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• 1974

This paper is proposed to describe a new species and two formae offlowering plants from central and southern Korea. The taxa of new plants are as follows: Miscanthus chejuensis Y. Lee, sp. nov. Rhododendron schlippenbachii Maxim. form. albiflorum Y. Lee, form. nov. Scilla scilloides (Lindl.) Druce form. albida Y. Lee, form. nov.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.198-202
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• 2013

To produce transgenic cucumber expressing Nit gene coffering abiotic resistance, the cotyledonary-node explants of cucumber (cv. Eunsung) were inoculated with A. tumefaciens transformed with pPZP211 or pCAMBIA2300 carrying Nit gene, that has cis-acting element involved in resistance to various abiotic environmental stresses. After co-cultivation, the procedures of selection, shoot initiation, shoot elongation, and plant regeneration were followed by cotyledonary-node transformation method (CTM, Jang et al. 2011). The putative transgenic plants were selected when shoots were grown to a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with 100 mg/L paromomycin as a selectable agent. The confirmation of transgenic cucumber was based on the genomic PCR, Southern blot analysis, RT-PCR, and Northern blot analysis. A 105 shoots (4.12%) selected from the selection mediums were obtained from 2,547 explants inoculated. Of them, putative transgenic plants were only confirmed with 45 plants (1.77%) by genomic PCR analysis. Transgenic plants showed that the Nit genes integrated into each genome of 39 plants (1.53%) by Southern blot analysis, and the expression of gene integrated into cucumber genome was only confirmed at 6 plants (0.24%) by RT-PCR and Northern blot analysis. These results lead us to speculate that the genes were successfully integrated and expressed in each genome of transgenic cucumber.

• Korean Journal of Plant Resources
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• v.12 no.3
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• pp.240-252
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• 1999

Floristic composition and phytosociological studies of Mt. Chongok and Mt. Tuta were investigated, and that was compared with the previously published report in 1993. Vascular plants were composed of 100 families, 358 genera, 573 species, 95 varieties, and 18 formae, totaling 686 taxa. The vegetation was relatively well conserved based on Pteridophyta calculation (Pte-Q), 1.13. Compared with the vascular plants of the southern and northern slope area, the vascular plants of the southern slope area were composed of 87 families, 287 genera, 419 species, 73 varieties, and 11 formae, totaling 503 taxa, and those of the northern slope area consisted of 94 families, 293 genera, 427 species, 73 varieties, and 12 formae, totaling 512 taxa, respectively. Also, compared with the taxa in each side, both sides were composed of 332 species in common, southern sides, 172 species and northern sides, 182 species, respectively. The number of species of 11 families belonged to the higher level among total families taxa was composed of 328 species(47.8%). Among them, Compositae and Rosaceae were included much more species than remnant families. Korean endemic species were composed 16 families, 24 genera, 20 species, 8 varieties and 2 formae, totaling 30 species(4.4%). Compared with the Korean endemic taxa in each side, both sides were composed of 14 species in common, southern sides, 11 species and northern sides, 5 species, respectively. A naturalized plants were 20 species, correspond to 9.2% of totaling 218 species appeared in South Korea. Among them,12 species were appeared commonly in both sides, southern sides, 16 species and northern sides, 16 species, respectively. Life form spectra was H-D1-R5-e type and, useful resources plants are as follows; edible source(42.4%), medicinal source(31.5%), ornamental source(15.6%) and pasture source (13.3%) in the total region. The forest vegetation of the southern slope was classified into 1 order, 1 alliances and 5 communities; Rhododendro-Quercetalia mongolicae, Lindero-Quercion mongolicae, Quercus mongolica Typical community, Populus davidiana-Quercus mongolica community, Pinus koraiensis-Taxus cuspidata community, Pinus densiflora-Carex humilis var. nana community, Betula costata-Betula ermanii community. It is considered that the slight difference of the flora and vegetation in the northern and southern slope is mostly due to the topographical and climatic difference. Even closer investigation is required for the more accurate comparison in this area.

• Journal of Korean Society of Forest Science
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• v.26 no.1
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• pp.31-41
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• 1975

This work was carried out from March 1963 to December 1974 to investigate exotic woody plants in the southern part of Korea. The author enumerated 263 kinds of woody plants; 203 species, 40 varieties, and 17 forms which belong to 136 genera and 63 families. Among 263 sorts of woody plants 99 were introduced from Japan, 87 from China, 32 from the North America, 17 from Europe, 13 from Australia, 8 from India and 8 from central part of Asia, etc. On the use of those trees of today 151 have been used for an ornamental source, 49 for timber, 22 for edible, 16 for medicinal and 22 for the others.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.101-106
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• 2002

This study was conducted to obtain the transformed tobacco (Nicotiana tubacum) plants with cytosolic ascorbate peroxidase gene(ApxSC7) using Agrobacterium tumefaciens LBA4404. A cDNA encoding the cytosolic ascorbate peroxidase of strawberry, ApxSC7, was introduced into tobacco plants via Agrobacterium-mediated gene transfer system. The expression vector, pIG-AP8, harboring ApxSC7 gene was used for production of transgenic tobacco plants. A large number of transgenic plants were regenerated on a medium containing hygromycin. Integration of ApxSC7 gene was confirmed by PCR and Southern blot analyses with genomic DNA. Northern blot analyses revealed that the pIGap8 gene was constitutively expressed.

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.188-194
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• 1998

This experiment was conducted to introduce phosphinothricin acetyl -transferase(PAT) gene, resistant to basta and non-selective herbidide, into tobacco(Nicotiana tabacum cv.BY4). For shoot formation,tobacco leaf disks were placed on the MS medium supplemented with 2.0mg/L BA and 0.1mg/L NAA. In this medium condition, tobacco leaf disces were cocultivated with A. tumefaciens MP90 containing NPT IIand PAT resistant to kanamycin and Basta, respectively. Shoots were obtained in the medium containing antibiotics, and those were transferred to rooting medium supplemented with 0.1mg/L NAA and antibiotics. The plants obtaining roots were transplanted into soil. Phenotype of transgenic tobacco plant was mostly as normal plant. However, about 5% was abnormal plant, which did not set seeds. PCR analysis and southern blot were performed to determine transformation. As the results, it was confirmed that PAT gene was stably integrated into tobacco genome.When herbicide, basta, was sprayed to the plants confirmed by PCR, the transgenic plants showed normal growth, whereas normal plants died. Therefore, the result of this experiment show that tobacco transformation for the resistance to basta, non-selective herbicide, was successful because PAT gene was stably integrated into tobacco.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.657-662
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• 2008

To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, the recombinant DgHSP17.2 gene was introduced into orchardgrass plants using the Agrobacterium-mediated transformation method and expressed constitutively under the control of the CaMV 35S promoter. The results of genomic DNA PCR and Southern analysis showed a DNA band and hybridization signal on agarose gel and X-ray film in transgenic orchardgrass plants harboring the recombinant DgHSP17.2 gene, but a DNA band and hybridization signal were not observed in the wild type and empty vector control plants. The same result was also obtained in RT-PCR and Southern blot analysis, and these transgenic orchardgrass plants did not show any morphological aberration both in the culture bottle and soil mixture. When leaf discs cut from transgenic orchardgrass plants with recombinant DgHsp17.2 gene were exposed to lethal temperature (heat treatment at $60^{\circ}C$ for 50 min), 60-80% of the leaf discs showed only damage symptoms, but non-transgenic leaf discs showed a lethal condition. These results indicate that the DgHsp17.2 gene may act as a protector from heat stress in plants.