• Archives of Pharmacal Research
• /
• v.25 no.6
• /
• pp.801-806
• /
• 2002

Cyclicpeptides are important targets in peptide synthesis because of their interesting biological properties. Constraining highly flexible linear peptides by cyclization is one of the mostly widely used approaches to define the bioactive conformation of peptides. Cyclic peptides often have increased receptor affinity and metabolic stability over their linear counterparts. We carried out virtual screening experiment via docking in order to understand the interaction between HLE-Human Leukocyte Elastase and ligand peptide and to identify the sequence that can be a target in various ligand peptides. We made cyclic peptides as a target base on Metlle-Phe sequence having affinity for ligand and receptor active site docking. There are three ways to cyclize certain sequences of amino acids such as Met-lie-Phe-Gly-Ile. First is head-to-tail cyclization method, linking between N-terminal and C-terminal. Second method utilizes amino acid side chain such as thiol functional group in Cys, making a thioether bond. The last one includes an application of resin-substituted amino acids in solid phase reaction. Among the three methods, solid phase reaction showed the greatest yield. Macrocyclization of Fmoc-Met-Ile-Phe-Gly-Ile-OBn after cleavage of Fmoc protection in solution phase was carried out to give macrocyclic compound 5 in about 7% yield. In the contrast with solution phase reaction, solid phase reaction for macrocyclization of Met-Ile-Phe-Gly-Ile-Asp-Tentagel in normal concentrated condition gave macrocyclic compound 7 in more than 35% yield.

• Journal of the Korean Ceramic Society
• /
• v.49 no.3
• /
• pp.253-259
• /
• 2012

In this study, crystal structural changes and dielectric properties of $(1-x)Pb(Yb_{1/2}Nb_{1/2})O_3-xPbTiO_3$ ((1-x)PYN-xPT) solid solutions were measured and analyzed with respect to the $PbTiO_3$ (PT) contents ($0.40{\leq}x{\leq}0.60$). From X-ray diffraction (XRD) measurements, (1-x)PYN-xPT solid solutions showed changes of the crystal structure from pseudocubic ($0.40{\leq}x{\leq}0.44$) to tetragonal ($0.52{\leq}x{\leq}0.60$) on increasing PT contents and exhibited the coexistence of pseudocubic and tetragonal phase near the morphotropic phase boundary (MPB) composition ($0.46{\leq}x{\leq}0.50$). The dielectric constant showed a maximum value at x = 0.46 and the maximum values in (1-x)PYN-xPT decreased with higher PT contents. The phase transition temperatures of (1-x)PYN-xPT solid solutions increased over the whole composition ranges tested ($0.40{\leq}x{\leq}0.60$).

• 한국연소학회:학술대회논문집
• /
• 2003.05a
• /
• pp.119-127
• /
• 2003

In this study we propose an unsteady I-dimensional model of bed combustion with multiple solid phases, which confers a phase on each solid material. This model can be applied to a variety of bed combustion cases of various configurations and ignition methods. It contains fuel combustion, gaseous reaction, heat transfers between each phase, and geometric changes of the solid particles. An iron ore sintering pot is selected for verifying the model validity and simulation results are compared with the limited experimental data set of various coke contents and air supply rates. They predict the experimental results well and show applicabilities to the various system of the fuel bed with various solid materials.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 2003.10a
• /
• pp.261-264
• /
• 2003

Semi-solid processing is now becoming of great interest for the production of various parts by pressure die casting. Many advantages are associated with this forming process at the condition that the forming operation is performed under appropriate conditions. The thixoforming process, which needs a suspension of a globular, non-dendritic solid phase in the liquid phase, is characterized by three major steps. The first step is casting of billets with a microstructure suited for thixoforming. The second step is reheating of slugs cut from these billets. The third step is injection of the semi-solid slugs into a die. In this paper, the horizontal reheating machine to obtain the optimal conditions suitable for semiu-solid die casting process was used and applied to extrusion material. It is estimated the possibility of application on semi-solid die casting with extrusion material through various results.

• The Pure and Applied Mathematics
• /
• v.19 no.2
• /
• pp.147-169
• /
• 2012

We introduce a Heaviside-function formulation of the interaction between incompressible two-phase fluid and a non-deformable solid. Fluid and solid interact in two ways : fluid satises the Dirichlet boundary condition imposed by the velocity field of solid, and solid is accelerated by the surface traction exerted by fluid. The two-way couplings are formulated by the Heaviside function to the interface between solid and fluid. The cumbersome treatment of interface is taken care of by the Heaviside function, and the interaction is discretized in a simple manner. The discretization results in a stable and accurate projection method.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 1997.03a
• /
• pp.159-164
• /
• 1997

The optimal conditions were investigated in order to manufacture the light automotive body parts using the semi-solid forging process by the finite element nalysis. Considering about macro-segregation cause to difference of relative velocity between solid phase and liquid phase, solidificational phenomenon cause to heat transfer from die and export of the latent heat, so solid fraction updating algorithm can be proposed. The rigid thermo-viscoplastic finite element analysis was carried out according to die temperature with proposed algorithm, so availability of forming part were understood. The finite element program can be used to the analysis of semi solid forging process.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.2
• /
• pp.271-279
• /
• 2004

The aroma component of Hallabong peel has been characterized by GC-MS with two different extraction techniques: solid-phase trapping solvent extraction (SPTE) and headspace solid-phase microextraction (HSSPME). Aroma components emitted from Hallabong peel were compared with those of other citrus varieties: lemon, orange and grapefruit by SPTE and GC-MS. d-Limonene (96.98%) in Hallabong was the main component, and relatively higher peaks of cis- ${\beta}$-ocimene, valencene and -farnesene were observed. Other volatile aromas, such as sabinene, isothujol and ${\delta}$-elemene were observed as small peaks. Also, principal components analysis was employed to distinguish citrus aromas based on their chromatographic data. For HSSPME, the fiber efficiency was evaluated by comparing the partition coefficient ($K_{gs}$Kgs) between the HS gaseous phase and HS-SPME fiber coating, and the relative concentration factors (CF) of the five characteristic compounds of the four citrus varieties. 50/30 ${\mu}$m DVB/CAR/PDMS fiber was verified as the best choice among the four fibers evaluated for all the samples.

• Journal of Environmental Science International
• /
• v.24 no.7
• /
• pp.841-849
• /
• 2015

The solid phase extractant SAN-D2EHPA/TBP containing two extractants of Di-(2-ethylhexyl)phosphoric acid (D2EHPA) and Tri-butyl-phosphate (TBP) was prepared by immobilizing two exractants D2EHPA and TBP in styrene acrylonitrile copolymer (SAN). The prepared SAN-D2EHPA/TBP was characterized by using fourier transform infrared spectrometer (FTIR) and scanning electron microscopy (SEM). The solid phase extractant SAN-D2EHPA/TBP was tested for the removal of Cu(II) from aqueous solution. Experiments were carried out as a function of the pH and Cu(II) concentration in the aqueous phase. The equilibrium time was 180 min and equilibrium experiment data obeyed the pseudo-second-order kinetic model. The Langmuir isotherm model represented the experiment data as well. The maximum removal capacity of Cu(II) calculated from Langmuir isotherm model was 3.1 mg/g.

• Korean Journal of Pharmacognosy
• /
• v.43 no.3
• /
• pp.250-256
• /
• 2012

In order to extract the curcuminoid such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric (Curcuma longa), solvent extraction methods (dipping and ultrasonic extraction method) and solid-phase extraction (SPE) were used. RP-HPLC (reverse-phase high-performance liquid chromatography) and TLC (thin-layer chromatography) were used for identification and analysis the three curcuminoid. From the experimental results, it is evident that the percentage of curcuminoid extracted from turmeric by ultrasonic extraction method was higher than dipping method. The percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanolic composition. Moreover, the total peak area of three curcuminoid was above 92% in RP-HPLC using solid-phase extraction. These results will form a database for investigating the constituents of natural products and the resources of pharmaceutical, nutrition, and cosmetic products.

• KSBB Journal
• /
• v.10 no.3
• /
• pp.335-342
• /
• 1995

Solid and liquid phase peroxidases were extracted from Chinese cabbage roots by using commercial juicer in order to use peroxidases from agricultural waste for industrial applications. Since peroxidases are distributed into 66% in liquid (juice) and 34% in solid phase (pulp), enzymes from both phases were applied to investigate the enzymatic removal of phenol from waste water. After contacting 150 ppm Phenol solution with liquid phase enzyme (1,800 unit/$\ell$) for 3 hours in a batch stirred reactor, 96% of phenol could be removed through polymerization and precipitation. Also, phenol could be removed from initial 120ppm to 5ppm by applying solid phase enzyme in an air lift reactor ($600 unit/\ell$). Almost equivalent efficiencies of phenol removal were observed between two systems, even though only one third of the enzymes in batch stirred reactor was applied in airlift reactor. The possible reason for this phenomenon is because peroxidases exist as immobilized forms in solid phase.