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Solid-Phase Extraction of Curcuminoid from Turmeric Using Physical Process Method  

Lee, Kwang-Jin (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Yang, Hye-Jin (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Jeong, Sang-Won (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Ma, Jin-Yeul (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.3, 2012 , pp. 250-256 More about this Journal
Abstract
In order to extract the curcuminoid such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric (Curcuma longa), solvent extraction methods (dipping and ultrasonic extraction method) and solid-phase extraction (SPE) were used. RP-HPLC (reverse-phase high-performance liquid chromatography) and TLC (thin-layer chromatography) were used for identification and analysis the three curcuminoid. From the experimental results, it is evident that the percentage of curcuminoid extracted from turmeric by ultrasonic extraction method was higher than dipping method. The percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanolic composition. Moreover, the total peak area of three curcuminoid was above 92% in RP-HPLC using solid-phase extraction. These results will form a database for investigating the constituents of natural products and the resources of pharmaceutical, nutrition, and cosmetic products.
Keywords
Turmeric; Curcuminoid; Solid-Phase Extraction; Ultrasonic wave;
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